Shop All Primary Cells

Human Pulmonary Artery Smooth Muscle Cells (HPASMC), Proliferating Culture, Prepared in Medium 231 and SMGS (Gibco™)

Quaternary cultures established from cryopreserved cells, grown to approximately 50% confluence in T-25 flasks.

Monkey (cynomolgus) Cryopreserved Hepatocytes, Plateable Male (Gibco™)

These plateable cryopreserved male Cynomolgus Monkey hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Pulmonary Artery Smooth Muscle Cells (HPASMC) (Gibco™)

Primary human pulmonary artery smooth muscle cells cryopreserved at the end of the tertiary culture.
• Viability: at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 16 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected
• Negative for von Willebrand factor (vWf)
• Positive for α-actin

Rat (Wistar) Cryopreserved Hepatocytes, Suspension Male (Gibco™)

These cryopreserved male rat (Wistar) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4–8 million

Get the Results You Need with GIBCO® Hepatocytes
High quality hepatocytes with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitroin vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure that you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:

• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes.

Learn about our full line of hepatocytes.

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Monkey (cynomolgus) Cryopreserved Hepatocytes, Male (Gibco™)

These cryopreserved male Cynomolgus Monkey hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

DR4 Mouse Embryonic Fibroblasts, irradiated (Gibco™)

Irradiated Gibco DR4 Mouse Embryonic Fibroblasts (MEFs) are multidrug-resistant feeder cells that support the culture of healthy undifferentiated human and mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. No. GSC-6204G, these MEF cells are isolated from DR4 x CF1 hybrid mice, mitotically arrested by irradiation, and tested to help ensure safety and performance. For other variants of drug-resistant MEFs, see CF6-Neo Mouse Embryonic Fibroblasts, Irradiated or CF6-Neo Mouse Embryonic Fibroblasts, MitC-Treated. You may also choose from our selection of non-drug-resistant MEFs to suit your application.

• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Perform drug selection with MEFs that are resistant to neomycin/geneticin, puromycin, hygromycin, and 6-thioguanine

Help save time with ready-to-use MEFs
Irradiated DR4 MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, as well as expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.

Culture with meticulously tested feeder cells
Each lot of DR4 MEFs is tested for bacteria, fungi, mycoplasma, and various mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ESCs to help ensure robust and consistent performance. These MEFs have successfully been used with ESC/iPSC lines from other species, but should be tested for compatibility if used for such an application.

Perform drug selection with multidrug-resistant cells
DR4 MEFs express transgenes that make them resistant to four different drugs: geneticin (G418), puromycin, hygromycin, and 6-thioguanine. Each lot is tested for tolerance to various concentrations of these drugs and the threshold range for survival is reported in the certificate of analysis to aid in designing experiments. Use these feeder cells to support undifferentiated ESC or iPSC culture while performing drug selection.

Mouse (CD-1) Cryopreserved Hepatocytes, Plateable Male (Gibco™)

These plateable cryopreserved male mouse (CD-1) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO® Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitroin vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure that you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Microvascular Endothelial Cells, neonatal dermis (HMVECnd), Proliferating Culture, Prepared in Medium 131 and MVGS (Gibco™)

Quaternary cultures established from cryopreserved cells, grown to approximately 50% confluence in T-25 flasks.

Primary Mouse Cortical Neurons (Gibco™)

Gibco Primary Mouse Cortical Neurons are isolated and cryopreserved from C57BL/6 embryonic day-17 mice. A flexible, ready-to-use, and quality alternative to fresh neurons, each vial contains 1 million cells per mL. These neurons consistently yield post-thaw viability in the range of 50-80%, have high neuronal purity, and are validated for functionality.

• High viability: post-thaw viability of 50% or greater
• High purity: greater than 98% neuronal phenotype
• Functionally validated: tested for long-term survival, neurotransmitter response, and transduction

Rat (Wistar) Cryopreserved Hepatocytes, Suspension Female

These cryopreserved female rat (Wistar) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4–8 million

Get the Results You Need with GIBCO® Hepatocytes
High quality hepatocytes with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitroin vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure that you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:

• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes.

Learn about our full line of hepatocytes.

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Mouse (CD-1) Cryopreserved Hepatocytes, Plateable Female (Gibco™)

These plateable cryopreserved female mouse (CD-1) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Rat (Sprague-Dawley) Cryopreserved Hepatocytes, Male (Gibco™)

These cryopreserved male rat (Sprague-Dawley) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Epidermal Keratinocytes, neonatal, Animal Product-Free (HEKn-APF) (Gibco™)

Primary human epidermal keratinoctyes from neonatal foreskin. Isolated, grown and cryopreserved at the end of the primary culture in an animal product-free environment.

• Viability at least 70%
• ≥500,000 viable cells⁄vial
• Capable of at least 30 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected

Human Suspension Hepatocytes, Transporter Qualified, (4-8 x 106 cells) (Gibco™)

These cryopreserved human hepatocytes are suitable for use in suspension metabolism applications.

Cells per vial: 4-8 million

Taurocholate, digoxin, and estradiol 17β glucuronide (E2-17G), substrates of the uptake and efflux transporters NTCP and BSEP, OATP1B3 and MDR1, OATPs and MRP2, respectively, are included in analyses

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Microvascular Endothelial Cells, neonatal dermis (HMVECnd) (Gibco™)

Primary human microvascular endothelial cells isolated from neonatal dermis, cryopreserved at the end of the tertiary culture.
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 16 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected
• Positive for von Willebrand factor (vWf)
• Positive for CD31
• Positive for CD36
• Negative for a-actin
• Positive for diI-Ac-LDL uptake