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Rat (Sprague-Dawley) Cryopreserved Hepatocytes, Plateable Male Gibco™

These plateable cryopreserved male rat (Sprague-Dawley) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, proper cell morphology, and metabolic function increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Viability ≥ 75%
• Attachment efficiency
• Monolayer formation and integrity
• Confluency >80% on Day 5
• Phase I enzyme activities

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Microvascular Endothelial Cells, neonatal dermis (HMVECnd) Gibco™

Primary human microvascular endothelial cells isolated from neonatal dermis, cryopreserved at the end of the tertiary culture.
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 16 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected
• Positive for von Willebrand factor (vWf)
• Positive for CD31
• Positive for CD36
• Negative for a-actin
• Positive for diI-Ac-LDL uptake

Human Plateable Hepatocytes, Uptake Qualified Gibco™

Cryopreserved human hepatocytes for use in transporter-mediated uptake applications.

Cells per vial: 4-8 million

Prototypical hepatic uptake transporter substrates taurocholate and estradiol-17β-glucuronide have been assessed to qualify the transport function of hepatic NTCP (SLC10A1) and OATP1B14/3 (SLCO1B1/3), respectively. The transport of each substrate (radiolabeled) was monitored by scintillation analysis and active transport values (37°C - 4°C) determined by linear regression.

Get the Results You Need with GIBCO® Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro/in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:

• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Link

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes

Human Plateable Hepatocytes, Metabolism Qualified Gibco™

These cryopreserved human hepatocytes are suitable for use in plated metabolism applications.

Cells per vial: 4-8 million

Prototypical cytochrome P450 substrates midazolam, tolbutamide, and dextromethorphan were used to assess the enzymatic function of CYP3A4⁄5, CYP2C9 and CYP2D6 respectively. The disappearance of parent was monitored by LC-MS⁄MS analysis and intrinsic clearance (CLint) values determined by linear regression.

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, proper cell morphology, and metabolic activity increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Viability ≥ 70%
• Attachment efficiency
• Monolayer formation and integrity
• Confluency >70% on Day 5
• Phase I enzyme activities
• Relevant CYP450 polymorphisms

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Skeletal Myoblasts (HSkM) Gibco™

Primary Normal Human Skeletal Myoblasts (HSkM) are designed to undergo highly efficient differentiation following plating of cryopreserved cells.

The cells are:
• Performance tested—guaranteed to differentiate ≥ 50% following 48 hours of incubation
• Guaranteed to be ≥ 70% viable
• Tested for mycoplasma, bacteria, yeast or other fungi, hepatitis B, hepatitis C, and HIV-1 viruses

Each vial contain sufficient number of cells to fully seed a single multi-well dish (ranging in format from 6-well to 384-well).

Rat (Sprague-Dawley) Cryopreserved Hepatocytes, Plateable Female Gibco™

These plateable cryopreserved female rat (Sprague-Dawley) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

HEP10, Pooled Human Cryopreserved Hepatocytes Gibco™

Get the convenience, affordability, and power of a pooled population of hepatocytes in a single vial. HEP10™ Pooled Human Hepatocytes are a representative population of 10 different donor hepatocytes that have been isolated using our novel proprietary CellStream Isolation Technology™, helping to deliver consistent, predictable results in your drug metabolism studies and other biological assays.

• Pure, highly viable hepatocytes isolated using our CellStream Isolation Technology™
• 10 donor hepatocytes, 5 male and 5 female in each vial
• Verified enzymatic and pathway activity

Each lot is tested for:
• Viability ≥ 75%
• Phase I and II enzyme activities
• Transporter suspension uptake

CellStream™ Isolation Technology
HEP10™ Pooled Human Hepatocytes are made using our novel proprietary CellStream Isolation Technology™. This process eliminates the disadvantages of density gradient fractionation prior to cryopreservation of the pool. Instead, we gently isolate and purify the pre-screened, high-viability, and metabolically-active hepatocytes from other cells and debris. The result is a remarkably pure pooled hepatocyte preparation with high viability for use in drug metabolism studies and other biological assays.

10 Donors in One Vial
Each vial of HEP10™ Pooled Human Hepatocytes contains hepatocytes from ten individual donors, 5 female and 5 male donors. Custom HEP10 lots can also be prepared from our broad array of high-quality, single-donor cells. Large lots are also available for extensive studies.

Verified Activity
Each pool is characterized for CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1, CYP3A4, CYP3A4⁄5, FMO activity, and NTCP, OATP1B3, and OATP transporter pathways.

Ready for Your Drug Metabolism Experiments
HEP10™ Pooled Human Hepatocytes are useful in a wide variety of applications, including intrinsic clearance, transport, toxicology, biochemistry and cell biology studies.,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Plateable Hepatocytes, 5-Donor Gibco™

These '5-Donor' Human Plateable Hepatocytes are a pooled population of primary hepatocytes produced by combining cells from 5 individual donors in a single vial. Pooling donors helps to minimize lot-to-lot variation and improves predictability of drug metabolism studies and other biological assays.

• Pure, highly viable, plateable hepatocytes
• Lot-specific characterization data
• Thawing and plating process compatible with existing protocols

Each lot is tested for:
• Attachment efficiency
• Monolayer formation and integrity
• Confluency >80% on Day 2
• Phase I enzyme activities
• Levels of CYP1A2, CYP2B6, and CYP3A4 induction (mRNA)
• Monolayer formation in medium with and without HepExtend (A2737501) supplement

Consistent cell attachment
High-quality plateable primary hepatocytes with high viabilities, proper cell morphology, and metabolic activity increase the ability to draw in vitro/in vivo correlations and make sound decisions regarding a compound’s fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

5 donors in a single vial
The cells are provided in a cryopreserved format similar to single donor plateable hepatocytes and have been shown to have approximately equal representation of each donor when plated.

Verified activity
Every lot of pooled plateable hepatocytes is tested for Phase I enzyme activity, cell viability, plating efficiency, and morphology.

Rat (Sprague-Dawley) Cryopreserved Hepatocytes, Male Gibco™

These cryopreserved male rat (Sprague-Dawley) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Dermal Fibroblasts, adult (HDFa) Gibco™

Primary Human Dermal Fibroblasts, adult (HDFa) are isolated from adult skin and cryopreserved at the end of the primary culture.

The cells are:
• Performance tested—at least 12 population doublings when cultured in Medium 106 supplemented with Low Serum Growth Supplement (LSGS)
• Guaranteed to be ≥ 70% viable
• Tested for mycoplasma, bacteria, yeast or other fungi, hepatitis B, hepatitis C, and HIV-1 viruses

Each vial contains over 0.5 x106 viable cells that have been cryopreserved at the end of the primary culture stage in a medium containing DMSO.

Human Suspension Hepatocytes, Metabolism Qualified, Male, (4-8 x 106 cells) Gibco™

These cryopreserved human male hepatocytes are suitable for use in suspension metabolism applications.

Cells per vial: 4-8 million

The commonly tested Phase I (P450) and Phase II (UGT, SULT) drug metabolizing enzymes were analyzed for metabolic activity in each lot. Metabolite formation was measured by standard biochemical assays using LC⁄MS⁄MS or HPLC.

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Cryopreserved Hepatocytes, Transporter Certified Gibco™

These plateable, cryopreserved human primary hepatocytes have been certified by Qualyst Transporter Solutions (QTS) for use with their patented B-CLEAR™ methodology for measurement of biliary efflux. QTS-certified hepatocytes not only maintain basic metabolism and morphology, but also express functioning uptake and efflux transporters.

Qualyst Transporter Solutions is the exclusive provider of hepatic drug transporter products and contract research services that utilize B-CLEAR technology. Their comprehensive hepatic cell-certification process includes the following analyses:

• A multi-point evaluation throughout the plating, overlay, and culture periods
• A quantitative evaluation of uptake and efflux transporter function
• Evaluation of total uptake, intracellular concentration, hepatic efflux, and overall clearance of each substrate
• Demonstration of functional inhibition of key transporters

Maintain basic function
Certification by this method helps to identify primary cells that have basic metabolism and morphology suitable for comprehensive transporter analysis.

Enhanced hepatic model
The B-CLEAR model is an exclusive hepatic model that combines both uptake and efflux transporters with hepatic metabolism.

Predict drug-drug interactions
Data from the B-CLEAR model can be used in a variety of ways, including in the prediction of clinically relevant drug interactions and in support of mechanisms of transporter interactions and hepatotoxicity.

Rat (Wistar) Cryopreserved Hepatocytes, Suspension Male Gibco™

These cryopreserved male rat (Wistar) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4–8 million

Get the Results You Need with GIBCO® Hepatocytes
High quality hepatocytes with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitroin vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure that you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:

• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes.

Learn about our full line of hepatocytes.

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Epidermal Keratinocytes, neonatal, Animal Product-Free (HEKn-APF) Gibco™

Primary human epidermal keratinoctyes from neonatal foreskin. Isolated, grown and cryopreserved at the end of the primary culture in an animal product-free environment.

• Viability at least 70%
• ≥500,000 viable cells⁄vial
• Capable of at least 30 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected

Human Epidermal Keratinocytes, adult (HEKa) Gibco™

Primary Normal Human Epidermal Keratinocytes, adult (HEKa) are isolated from adult skin and cryopreserved at the end of the primary culture.

The cells are:
• Performance tested—at least 25 population doublings when cultured in EpiLife Medium supplemented with HKGS (for HEKa) or Supplement S7 (for HEKa-AOF)
• Guaranteed to be ≥ 70% viable
• Tested for mycoplasma, bacteria, yeast or other fungi, hepatitis B, hepatitis C, and HIV-1 viruses

Each vial contains over 0.5 x106 viable cells that have been cryopreserved at the end of the primary culture stage in a medium containing 10% DMSO.
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