Shop All Primary Cells

CF6-Neo Mouse Embryonic Fibroblasts, MitC-treated Gibco™

Mitomycin C (MitC)-treated Gibco CF6-Neo Mouse Embryonic Fibroblasts (MEFs) are neomycin-resistant feeder cells that support healthy undifferentiated human and mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) in culture. Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. No. GSC-6005M, these MEF cells are isolated from CF1 x C57BL/6 hybrid mice, mitotically arrested by MitC-treatment, and tested to help ensure performance and minimize the risk of contamination. For neomycin-resistant MEFs that are inactivated by irradiation, see CF6-Neo Mouse Embryonic Fibroblasts, irradiated. You may also choose from our broad selection of other MEFs to suit your application.

• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Perform research involving drug selection using neomycin-resistant cells

Help save time with ready-to-use MEFs
MitC-treated CF6-Neo MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, and expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.

Culture with meticulously tested feeder cells
Each lot of CF6-Neo MEFs is tested for bacteria, fungi, mycoplasma, and various mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ES cells to help ensure robust and consistent performance in your research. These MEFs have successfully been used with ESC/iPSC lines from other species but should be tested for compatibility if used for such an application.

Perform research involving drug selection using neomycin-resistant cells
CF6-Neo MEFs express neoR from a pPGKneobPa transgene, making them highly resistant to neomycin and geneticin (G418). Each lot is tested for tolerance to various G418 concentrations, and the threshold range for survival is reported in the CoA to aid in designing experiments. Use these feeder cells to support undifferentiated ESC or iPSC culture while performing G418 selection.

Human Plateable Hepatocytes, Induction Qualified Gibco™

These plateable cryopreserved human hepatocytes are inducible and suitable for use in experiments monitoring in vitro enzyme induction.

Cells per vial: 4-8 million

The potentials of known clinically relevant enzyme inducers represented by 3-methylcholanthrene (3-MC, a prototypical CYP1A enzyme inducer, 3 µM), phenobarbital (PB, a prototypical CYP2B inducer, 1000 µM) and rifampicin (RIF, a prototypical CYP3A enzyme inducer, 10 µM) have been assessed to verify that each lot is inducible.

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro/in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes

Activated Human Myofibroblastic Hepatic Stellate Cells (MF-HSCs) Gibco™

Gibco Activated Human Myofibroblastic Hepatic Stellate Cells (MF-HSCs) are produced from isolated pure quiescent human stellate cells (q- HSCs). The cells are obtained from a single donor and provided in a cryopreserved format identical to single-donor plateable human hepatocytes.

Each vial contains a minimum of one million viable cells at passage 5, sufficient for at least four 96-well plates in 2D culture.

Simplified protocol
Activated MF-HSCs are suitable for building hepatic co-culture with primary hepatocytes to study in vitro disease models such as hepatic fibrosis. Cryopreserved cells can be thawed and plated at your convenience in as little as 30 minutes. Try it on your own, or request a demo with an experienced scientist.

Stringent quality control
Each lot is tested for:
• Viability >75%
• Attachment efficiency
• Proper morphology
• Purity based on α-smooth muscle actin (α-SMA)
• Activation in response to Transforming Growth Factor-β1 (TGF-β1)

Mouse (CD-1) Cryopreserved Hepatocytes, Plateable Male Gibco™

These plateable cryopreserved male mouse (CD-1) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO® Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitroin vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure that you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Epidermal Melanocytes, neonatal, darkly pigmented donor, (HEMn-DP), Proliferating Culture, Prepared in Medium 254 and HMGS Gibco™

Tertiary cultures established from cryopreserved cells, grown to approximately 50% confluence in T-25 flasks.

Human Astrocytes Gibco™

Gibco Human Astrocytes are normal human cells derived from human brain tissue. When used together with Gibco Astrocyte Medium, these astrocytes are highly mature and differentiated GFAP positive cells with the typical star-like morphology.

Gibco Human Astrocytes are cryopreserved at Passage 1. Each vial contains >1 million viable cells post-thaw.

B6-Puro Mouse Embryonic Fibroblasts, irradiated Gibco™

Irradiated Gibco B6-Puro Mouse Embryonic Fibroblasts (MEFs) are puromycin-resistant feeder cells that support the culture of healthy undifferentiated human and mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. No. GSC-6220G, these MEF cells are isolated from C57BL/6J-Tg(pPwL88puro)2EMS/J mice, mitotically arrested by irradiation, and tested to help ensure safety and performance. For other variants of drug-resistant MEFs, see CF6-Neo Mouse Embryonic Fibroblasts, Irradiated or DR4 Mouse Embryonic Fibroblasts, Irradiated. You may also choose from our selection of non-drug-resistant MEFs to suit your application.

• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Perform drug selection with puromycin-resistant cells

Help save time with ready-to-use MEFs
Irradiated B6-Puro MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, as well as expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.

Culture with meticulously tested feeder cells
Each lot of B6-Puro MEFs is tested for bacteria, fungi, mycoplasma, and various mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ES cells to ensure robust and consistent performance. These MEFs have successfully been used with ESC/iPSC lines from other species, but should be tested for compatibility if used for such an application.

Perform drug selection with puromycin-resistant cells
B6-Puro MEFs express puromycin N-acetyltransferase (pac) from a pPWL88puro transgene, making them highly resistant to puromycin. Each lot is tested for tolerance to various puromycin concentrations, and the threshold range for survival is reported in the certificate of analysis to aid in designing experiments. Use these feeder cells to support undifferentiated ESC or iPSC culture while performing puromycin selection.

Human Plateable Hepatocytes, Metabolism Qualified Gibco™

These cryopreserved human hepatocytes are suitable for use in plated metabolism applications.

Cells per vial: 4-8 million

Prototypical cytochrome P450 substrates midazolam, tolbutamide, and dextromethorphan were used to assess the enzymatic function of CYP3A4⁄5, CYP2C9 and CYP2D6 respectively. The disappearance of parent was monitored by LC-MS⁄MS analysis and intrinsic clearance (CLint) values determined by linear regression.

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Rat (Sprague-Dawley) Cryopreserved Hepatocytes, Plateable Male Gibco™

These plateable cryopreserved male rat (Sprague-Dawley) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Primary Rat Cortex Neurons, Sprague Dawley Gibco™

Gibco Primary Rat Cortex Neurons, Sprague Dawley, are isolated and cryopreserved from Sprague Dawley embryonic day-18 rats. A flexible, ready-to-use, and quality alternative to fresh neurons, each vial contains one million cells per mL. These neurons consistently yield post-thaw viability in the range of 50–80%, have high neuronal purity, and are validated for functionality.

High viability: post-thaw viability of 50% or greater
High purity: greater than 90% neuronal phenotype
Functionally validated: tested for spontaneous synchronous calcium flux and synaptic release activity

Human Suspension Hepatocytes, Metabolism Qualified, Female, (4-8 x 106 cells) Gibco™

These cryopreserved human female hepatocytes are suitable for use in suspension metabolism applications.

Cells per vial: 4–8 million

The commonly tested Phase I (P450) and Phase II (UGT, SULT) drug metabolizing enzymes were analyzed for metabolic activity in each lot. Metabolite formation was measured by standard biochemical assays using LC/MS/MS or HPLC.

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitroin vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

C57BL/6 Mouse Embryonic Fibroblasts, irradiated Gibco™

Irradiated Gibco C57BL/6 Mouse Embryonic Fibroblasts (MEFs) are feeder cells that support the culture of healthy undifferentiated embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), especially mouse ESCs/iPSCs. Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. Nos. GSC-6002G and GSC-6202G, these MEF cells are isolated from inbred C57BL/6 mice, mitotically arrested by irradiation, and tested to help ensure performance and minimize the risk of contamination. For our most widely used feeder cells for culturing pluripotent stem cells, see CF1 Mouse Embryonic Fibroblasts, Irradiated. You may also choose from our selection of drug-resistant MEFs to suit your application.

• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Provide optimum support for mouse ESCs and iPSCs

Help save time with ready-to-use MEFs
Irradiated C57BL/6 MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, and expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.

Culture with meticulously tested feeder cells
Each lot of irradiated C57BL/6 MEFs is tested for bacteria, fungi, mycoplasma, and mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ESCs to help ensure robust and consistent performance. Culture your ESCs and iPSCs with confidence, knowing that you can rely on these high quality feeder cells.

Provide optimum support for mouse ESCs and iPSCs
While C57BL/6 MEFs can also support human ESCs and iPSCs, these feeder cells work particularly well for culturing mouse ESCs and iPSCs, showing better results compared to CF1 and ICR strain MEFs.

Mouse (CD-1) Cryopreserved Hepatocytes, Plateable Female Gibco™

These plateable cryopreserved female mouse (CD-1) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Rat (Wistar) Cryopreserved Hepatocytes, Plateable Male Gibco™

These plateable cryopreserved male rat (Wistar) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4–8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:

• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Astrocytes Kit Gibco™

The Gibco Human Astrocytes Kit contains normal human cells derived from human brain tissue in addition to Gibco Astrocyte Medium. When cultured with Gibco Astrocyte Medium, these astrocytes are highly mature and differentiated GFAP positive cells with the typical star-like morphology.

Gibco Human Astrocytes are cryopreserved at Passage 1. Each vial contains >1 million viable cells post-thaw.
Results per page
    spinner