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Flp-In™-CHO Cell Line (Invitrogen™)

Flp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. Targeted integration of a Flp-In™ expression vector ensures high-level expression of your gene of interest. There are six Flp-In™ Cell Lines available for the generation of isogenic stable cell lines and a Flp-In™ T-REx™ Cell Line for the generation of tetracycline-regulated stable cell lines. Flp-In™-CV-1, Flp-In™-293, Flp-In™-BHK, Flp-In™-Jurkat, and Flp-In™-3T3 Cell Lines were created by transfecting the parent cell lines with pFRT/lacZeo and selecting for stable Zeocin™- resistant clones. The Flp-In™-CHO Cell Line was created by transfecting CHO cells with pFRT/lacZeo2 and selecting for Zeocin™-resistant clones. The Flp-In™ T-REx™-293 Cell Line contains pFRT/lacZeo and pcDNA™6/TR (from the T-REx™ System) stably integrated. Co-transfection of the Flp-In™ Cell Lines with a Flp-In™ expression vector and the Flp recombinase vector, pOG44, results in targeted integration of the expression vector to the same locus in every cell, ensuring homogeneous levels of gene expression.

Choosing your Flp-In™ Vector/Cell Line Combination
The Flp-In™-CV-1, Flp-In™-293, Flp-In™-CHO, and Flp-In™-Jurkat Cell Lines (Figure 1) work well with Flp-In™ vectors that express a gene from the CMV promoter (pcDNA™5/FRT, pcDNA5/FRT/V5-His-TOPO®, and pSecTag/FRT/V5-His-TOPO®). Flp-In™-BHK and Flp-In™-3T3 cells tend to down regulate the CMV promoter. Therefore, it is recommended that the Flp-In™ vectors containing the EF-1α promoter (pEF5/FRT/V5-DEST™ and pEF5/FRT/V5-D-TOPO®) be used with these cell lines.

293FT Cell Line (Invitrogen™)

The 293FT Cell line is ideal for generating high-titer lentivirus using the ViraPower™ Lentiviral Expression System. The 293FT Cell Line is a fast-growing, highly transfectable clonal isolate derived from human embryonal kidney cells transformed with the SV40 large T antigen. When a ViraPower™ expression vector and the ViraPower™ Lentiviral Packaging Mix are co-transfected into 293FT cells, high levels of the viral RNA and the gag⁄pol and rev proteins required for packaging are produced.

Advantages
• Fast growing
• Highly transfectable
• Produces high viral titers
• Allows very high levels of protein

Key Features
• Presence of the SV40 large T antigen allows very high levels of protein to be expressed from vectors containing the SV40 origin. Higher, in fact, than other 293 cells or the T antigen-transformed COS cells classically used.

Kit includes
• 293FT Cell Line provided as a frozen stock in 1 ml of 90% complete media and 10% DMSO

Flp-In™-BHK Cell Line (Invitrogen™)

Flp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. Targeted integration of a Flp-In™ expression vector ensures high-level expression of your gene of interest. There are six Flp-In™ Cell Lines available for the generation of isogenic stable cell lines and a Flp-In™ T-REx™ Cell Line for the generation of tetracycline-regulated stable cell lines. Flp-In™-CV-1, Flp-In™-293, Flp-In™-BHK, Flp-In™-Jurkat, and Flp-In™-3T3 Cell Lines were created by transfecting the parent cell lines with pFRT/lacZeo and selecting for stable Zeocin™- resistant clones. The Flp-In™-CHO Cell Line was created by transfecting CHO cells with pFRT/lacZeo2 and selecting for Zeocin™-resistant clones. The Flp-In™ T-REx™-293 Cell Line contains pFRT/lacZeo and pcDNA™6/TR (from the T-REx™ System) stably integrated. Co-transfection of the Flp-In™ Cell Lines with a Flp-In™ expression vector and the Flp recombinase vector, pOG44, results in targeted integration of the expression vector to the same locus in every cell, ensuring homogeneous levels of gene expression.

Choosing your Flp-In™ Vector/Cell Line Combination
The Flp-In™-CV-1, Flp-In™-293, Flp-In™-CHO, and Flp-In™-Jurkat Cell Lines (Figure 1) work well with Flp-In™ vectors that express a gene from the CMV promoter (pcDNA™5/FRT, pcDNA5/FRT/V5-His-TOPO®, and pSecTag/FRT/V5-His-TOPO®). Flp-In™-BHK and Flp-In™-3T3 cells tend to down regulate the CMV promoter. Therefore, it is recommended that the Flp-In™ vectors containing the EF-1α promoter (pEF5/FRT/V5-DEST™ and pEF5/FRT/V5-D-TOPO®) be used with these cell lines.

Human OCT1 SLC Transporter Cells (Gibco™)

TRANSiPORT Human OCT1 SLC Transporter Cells are HEK293 cells that transiently overexpress the OCT1 solute carrier (SLC) transport protein. Solute carriers are a super-family of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes in and out of cells. The OCT1 SLC transporter protein is found in hepatocytes.

• Assess potential for transporter-mediated drug metabolism
• Easy to use format
• Obtain high quality results with a large signal-to-noise ratio

Choice of measurement system
Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.

Assay reliability
Mock HEK293 cells (Cat. No. GM1001) that do not overexpress the SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of transport due to the presence of endogenous transporters or non-specific binding.

Rapid results
The convenient product format enables data generation in 2 days from thawing of the cells to final results.

Related products
GM1002 Human OATP1B1 Transporter Cells
GM1003 Human OAT1 Transporter Cells
GM1004 Human OAT3 Transporter Cells
GM1005 Human OCT2 Transporter Cells
GM1006 Human OATP1B3 Transporter Cells
GM1013 Human NTCP Transporter Cells
GM1014 Human MATE1 Transporter Cells
GM1015 Human MATE2K Transporter Cells
GM1001 Mock Cells

KM71H, Pichia pastoris Yeast Strain (Invitrogen™)

Several Pichia pastoris strains are available to allow you to optimize expression and recovery of your protein of interest. Table 1 provides information to help you choose the appropriate strain.

Mock SLC Transporter Cells (Gibco™)

These cells are intended for use as a negative control in experiments utilizing TRANSiPORT solute carrier (SLC) transporter-overexpressing HEK293 cells. These mock cells do not overexpress the SLC transport protein and so can be used to determine levels of endogenous transport activity as well as non-specific binding of compounds being tested.

Related products
GM1002 Human OATP1B1 Transporter Cells
GM1003 Human OAT1 Transporter Cells
GM1004 Human OAT3 Transporter Cells
GM1005 Human OCT2 Transporter Cells
GM1006 Human OATP1B3 Transporter Cells
GM1008 Human OCT1 Transporter Cells
GM1013 Human NTCP Transporter Cells
GM1014 Human MATE1 Transporter Cells
GM1015 Human MATE2K Transporter Cells

T-REx™-CHO Cell Line (Invitrogen™)

T-REx™ Cell Lines stably express the tetracycline repressor protein (Table 1). They save significant time and effort when using the T-REx™ System. The T-REx™ Cell Lines are functionally tested by transient transfection with the positive control vector pcDNA™4⁄TO⁄lacZ. T-REx™ Cell Lines exhibit extremely low basal expression levels in the repressed state and high expression upon induction with tetracycline (Figure 1).

CTS™ Viral Production Cells (Gibco™)

CTS Viral Production Cells are derived from the human HEK293 cell line and are a core component of the CTS LV-MAX lentiviral production system to produce maximal lentiviral titers. The cells allow high density, serum-free suspension culture with minimal cell clumping in CTS LV-MAX Production Medium. They are specifically validated for lentiviral vector production for the purpose of cell and gene therapy applications, but may be suitable for the production of other viral vectors where the absence of SV40 large T antigen in HEK293 cells is desired. These cGMP-banked cells have gone through a documented validation process to ensure that they can be transfected with multiple plasmids and that viable viral particles can be produced.

Features of the CTS Viral Production Cells include:
• Fully-documented cGMP-banked cell line (HEK293F-derived) with detailed cell line lineage history
• Extensive quality testing based on the US FDA and European Medicines Agency (EMA) guidances for therapeutic use
• Flexible licensing options without royalties

Contact us (outlicensing@thermofisher.com) for more information and access.

Flp-In™-3T3 Cell Line (Invitrogen™)

Flp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. Targeted integration of a Flp-In™ expression vector ensures high-level expression of your gene of interest. There are six Flp-In™ Cell Lines available for the generation of isogenic stable cell lines and a Flp-In™ T-REx™ Cell Line for the generation of tetracycline-regulated stable cell lines. Flp-In™-CV-1, Flp-In™-293, Flp-In™-BHK, Flp-In™-Jurkat, and Flp-In™-3T3 Cell Lines were created by transfecting the parent cell lines with pFRT/lacZeo and selecting for stable Zeocin™- resistant clones. The Flp-In™-CHO Cell Line was created by transfecting CHO cells with pFRT/lacZeo2 and selecting for Zeocin™-resistant clones. The Flp-In™ T-REx™-293 Cell Line contains pFRT/lacZeo and pcDNA™6/TR (from the T-REx™ System) stably integrated. Co-transfection of the Flp-In™ Cell Lines with a Flp-In™ expression vector and the Flp recombinase vector, pOG44, results in targeted integration of the expression vector to the same locus in every cell, ensuring homogeneous levels of gene expression.

Choosing your Flp-In™ Vector/Cell Line Combination
The Flp-In™-CV-1, Flp-In™-293, Flp-In™-CHO, and Flp-In™-Jurkat Cell Lines (Figure 1) work well with Flp-In™ vectors that express a gene from the CMV promoter (pcDNA™5/FRT, pcDNA5/FRT/V5-His-TOPO®, and pSecTag/FRT/V5-His-TOPO®). Flp-In™-BHK and Flp-In™-3T3 cells tend to down regulate the CMV promoter. Therefore, it is recommended that the Flp-In™ vectors containing the EF-1α promoter (pEF5/FRT/V5-DEST™ and pEF5/FRT/V5-D-TOPO®) be used with these cell lines.

Human NTCP SLC Transporter Cells (Gibco™)

TRANSiPORT Human NTCP SLC Transporter Cells are HEK293 cells that transiently overexpress the NTCP solute carrier (SLC) transport protein. Solute carriers are a super-family of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes in and out of cells. The NTCP SLC transporter protein is found in hepatocytes.

• Assess potential for transporter-mediated drug metabolism
• Easy to use format
• Obtain high quality results with a large signal-to-noise ratio

Choice of measurement system
Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.

Assay reliability
Mock HEK293 cells (Cat. No. GM1001) that do not overexpress the SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of transport due to the presence of endogenous transporters or non-specific binding.

Rapid results
The convenient product format enables data generation in 2 days from thawing of the cells to final results.

Related products
GM1002 Human OATP1B1 Transporter Cells
GM1003 Human OAT1 Transporter Cells
GM1004 Human OAT3 Transporter Cells
GM1005 Human OCT2 Transporter Cells
GM1006 Human OATP1B3 Transporter Cells
GM1008 Human OCT1 Transporter Cells
GM1014 Human MATE1 Transporter Cells
GM1015 Human MATE2K Transporter Cells
GM1001 Mock Cells

SMD1168H, Pichia pastoris Yeast Strain (Invitrogen™)

Several Pichia pastoris strains are available to allow you to optimize expression and recovery of your protein of interest. Table 1 provides information to help you choose the appropriate strain.

Human OCT2 SLC Transporter Cells (Gibco™)

TRANSiPORT Human OCT2 SLC Transporter Cells are HEK293 cells that transiently overexpress the OCT2 solute carrier (SLC) transport protein. Solute carriers are a super-family of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes in and out of cells. The OCT2 SLC transporter protein is found in kidney proximal tubule cells.

• Assess potential for transporter-mediated drug metabolism
• Easy to use format
• Obtain high quality results with a large signal-to-noise ratio

Choice of measurement system
Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.

Assay reliability
Mock HEK293 cells (Cat. No. GM1001) that do not overexpress the SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of transport due to the presence of endogenous transporters or non-specific binding.

Rapid results
The convenient product format enables data generation in 2 days from thawing of the cells to final results.

Related products
GM1002 Human OATP1B1 Transporter Cells
GM1003 Human OAT1 Transporter Cells
GM1004 Human OAT3 Transporter Cells
GM1006 Human OATP1B3 Transporter Cells
GM1008 Human OCT1 Transporter Cells
GM1013 Human NTCP Transporter Cells
GM1014 Human MATE1 Transporter Cells
GM1015 Human MATE2K Transporter Cells
GM1001 Mock Cells

Human OATP1B1 SLC Transporter Cells (Gibco™)

TRANSiPORT Human OATP1B1 SLC Transporter Cells are HEK293 cells that transiently overexpress the OATP1B1 solute carrier (SLC) transport protein. Solute carriers are a super-family of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes in and out of cells. The OATP1B1 SLC transporter protein is found in hepatocytes.

• Assess potential for transporter-mediated drug metabolism
• Easy to use format
• Obtain high quality results with a large signal-to-noise ratio

Choice of measurement system
Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.

Assay reliability
Mock HEK293 cells (Cat. No. GM1001) that do not overexpress the SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of transport due to the presence of endogenous transporters or non-specific binding.

Rapid results
The convenient product format enables data generation in 2 days from thawing of the cells to final results.

Related products
GM1003 Human OAT1 Transporter Cells
GM1004 Human OAT3 Transporter Cells
GM1005 Human OCT2 Transporter Cells
GM1006 Human OATP1B3 Transporter Cells
GM1008 Human OCT1 Transporter Cells
GM1013 Human NTCP Transporter Cells
GM1014 Human MATE1 Transporter Cells
GM1015 Human MATE2K Transporter Cells
GM1001 Mock Cells

Sf21 Cells in Grace's (Gibco™)

Gibco® Sf21 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. The cells originated at the USDA Insect Pathology Laboratory, where they were derived from the pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. Gibco® Sf21 cells (frozen in Grace's Insect Medium) feature:
• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Quality and performance testing

Recombinant protein expression from a variety of expression systems
High levels of protein expression in Sf21 cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.

Good growth in adherent or suspension culture
Protocols for adherent or suspension growth in Gibco® Supplemented Grace's Insect Medium are available in the product manual. Cultures can easily be transferred between the two conditions for ease of workflow.

Quality and performance testing
Each lot of Gibco® Sf21 cells is tested for cell growth and viability post-recovery from cryopreservation.

Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.

T-REx™ Jurkat Cell Line (Invitrogen™)

T-REx™ Cell Lines stably express the tetracycline repressor protein (Table 1). They save significant time and effort when using the T-REx™ System. The T-REx™ Cell Lines are functionally tested by transient transfection with the positive control vector pcDNA™4⁄TO⁄lacZ. T-REx™ Cell Lines exhibit extremely low basal expression levels in the repressed state and high expression upon induction with tetracycline (Figure 1).