Shop All Mammalian Cell Lines

Flp-In™-293 Cell Line (Invitrogen™)

Flp-In™ 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. Targeted integration of a Flp-In™ expression vector ensures high-level expression of your gene of interest. Flp-In™-293 cell line was created by transfecting the parent cell lines with pFRT⁄lacZeo and selecting for stable Zeocin™- resistant clones.

Flp-In™-293 cells work well with Flp-In™ vectors that express a gene from the CMV promoter (e.g., pcDNA™5⁄FRT, pcDNA5⁄FRT⁄V5-His-TOPO®, and pSecTag⁄FRT⁄V5-His-TOPO

Flp-In™-CV-1 Cell Line (Invitrogen™)

Flp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. Targeted integration of a Flp-In™ expression vector ensures high-level expression of your gene of interest. There are six Flp-In™ Cell Lines available for the generation of isogenic stable cell lines and a Flp-In™ T-REx™ Cell Line for the generation of tetracycline-regulated stable cell lines. Flp-In™-CV-1, Flp-In™-293, Flp-In™-BHK, Flp-In™-Jurkat, and Flp-In™-3T3 Cell Lines were created by transfecting the parent cell lines with pFRT/lacZeo and selecting for stable Zeocin™- resistant clones. The Flp-In™-CHO Cell Line was created by transfecting CHO cells with pFRT/lacZeo2 and selecting for Zeocin™-resistant clones. The Flp-In™ T-REx™-293 Cell Line contains pFRT/lacZeo and pcDNA™6/TR (from the T-REx™ System) stably integrated. Co-transfection of the Flp-In™ Cell Lines with a Flp-In™ expression vector and the Flp recombinase vector, pOG44, results in targeted integration of the expression vector to the same locus in every cell, ensuring homogeneous levels of gene expression.

Choosing your Flp-In™ Vector/Cell Line Combination
The Flp-In™-CV-1, Flp-In™-293, Flp-In™-CHO, and Flp-In™-Jurkat Cell Lines (Figure 1) work well with Flp-In™ vectors that express a gene from the CMV promoter (pcDNA™5/FRT, pcDNA5/FRT/V5-His-TOPO®, and pSecTag/FRT/V5-His-TOPO®). Flp-In™-BHK and Flp-In™-3T3 cells tend to down regulate the CMV promoter. Therefore, it is recommended that the Flp-In™ vectors containing the EF-1α promoter (pEF5/FRT/V5-DEST™ and pEF5/FRT/V5-D-TOPO®) be used with these cell lines.

Flp-In™ T-REx™ 293 Cell Line (Invitrogen™)

Flp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that ensure homogenous expression of your protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. Targeted integration of a Flp-In™ expression vector ensures high-level expression of your gene of interest. The Flp-In™ T-REx™-293 Cell Line contains pFRT⁄lacZeo and pcDNA™6⁄TR (from the T-REx™ System) stably integrated. Co-transfection of the Flp-In™ Cell Lines with a Flp-In™ expression vector and the Flp recombinase vector, pOG44, results in targeted integration of the expression vector to the same locus in every cell, ensuring homogeneous levels of gene expression.

The Flp-In™-293 work well with Flp-In™ vectors that express a gene from the CMV promoter (e.g., pcDNA™5⁄FRT, pcDNA5⁄FRT⁄V5-His-TOPO®, and pSecTag⁄FRT⁄V5-His-TOPO®).

Flp-In™-CHO Cell Line (Invitrogen™)

Flp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. Targeted integration of a Flp-In™ expression vector ensures high-level expression of your gene of interest. There are six Flp-In™ Cell Lines available for the generation of isogenic stable cell lines and a Flp-In™ T-REx™ Cell Line for the generation of tetracycline-regulated stable cell lines. Flp-In™-CV-1, Flp-In™-293, Flp-In™-BHK, Flp-In™-Jurkat, and Flp-In™-3T3 Cell Lines were created by transfecting the parent cell lines with pFRT/lacZeo and selecting for stable Zeocin™- resistant clones. The Flp-In™-CHO Cell Line was created by transfecting CHO cells with pFRT/lacZeo2 and selecting for Zeocin™-resistant clones. The Flp-In™ T-REx™-293 Cell Line contains pFRT/lacZeo and pcDNA™6/TR (from the T-REx™ System) stably integrated. Co-transfection of the Flp-In™ Cell Lines with a Flp-In™ expression vector and the Flp recombinase vector, pOG44, results in targeted integration of the expression vector to the same locus in every cell, ensuring homogeneous levels of gene expression.

Choosing your Flp-In™ Vector/Cell Line Combination
The Flp-In™-CV-1, Flp-In™-293, Flp-In™-CHO, and Flp-In™-Jurkat Cell Lines (Figure 1) work well with Flp-In™ vectors that express a gene from the CMV promoter (pcDNA™5/FRT, pcDNA5/FRT/V5-His-TOPO®, and pSecTag/FRT/V5-His-TOPO®). Flp-In™-BHK and Flp-In™-3T3 cells tend to down regulate the CMV promoter. Therefore, it is recommended that the Flp-In™ vectors containing the EF-1α promoter (pEF5/FRT/V5-DEST™ and pEF5/FRT/V5-D-TOPO®) be used with these cell lines.

HepaRG™ Cells, Cryopreserved (Gibco™)

The HepaRG™ cell line is an immortalized hepatic cell line that retains many characteristics of primary human hepatocytes. HepaRG™ cells are terminally differentiated and provided in a convenient cryopreserved format. For scientists who need reproducible metabolism data, HepaRG™ cells are an in vitro tool that provides reproducible results in a metabolically complete and scalable system.
• Obtain biologically relevant results from a metabolically complete system
• Assess the drug-drug interaction potential of your compound
• Experience reproducible results from a single population of cells

Biologically Relevant
HepaRG™ cells exhibit many characteristics of primary human hepatocytes including morphology, expression of key metabolic enzymes, expression of nuclear receptors, and drug transporters. Unlike HepG2 and Fa2N-4 cells, HepaRG™ cells have high P450 activity and complete expression of all nuclear receptors.

Predict Metabolism-based Drug-drug Interaction
HepaRG™ cells respond to prototypical P450 inducers and inhibitors to the same extent as primary hepatocytes, allowing HepaRG™ cells to be used to evaluate potential drug-drug interactions.

Infinitely Reproducible
HepaRG™ cells exhibit many characteristics of primary human hepatocytes and are essentially a single donor. These features allow users to obtain physiological relevant results for metabolism-based drug-drug interactions without the concern of donor variability and limited lot sizes that come with relying on donor tissue. (Note: The cells we provide are terminally differentiated).

HepaRG™ is a trademark of BioPredic International.

Human MATE2K SLC Transporter Cells (Gibco™)

TRANSiPORT Human MATE2K SLC Transporter Cells are HEK293 cells that transiently overexpress the MATE2K solute carrier (SLC) transport protein. Solute carriers are a super-family of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes in and out of cells. The MATE2K SLC transporter protein is found in kidney proximal tubule cells.

• Assess potential for transporter-mediated drug metabolism
• Easy to use format
• Obtain high quality results with a large signal-to-noise ratio

Choice of measurement system
Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.

Assay reliability
Mock HEK293 cells (Cat. No. GM1001) that do not overexpress the SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of transport due to the presence of endogenous transporters or non-specific binding.

Rapid results
The convenient product format enables data generation in 2 days from thawing of the cells to final results.

Related products
GM1002 Human OATP1B1 Transporter Cells
GM1003 Human OAT1 Transporter Cells
GM1004 Human OAT3 Transporter Cells
GM1005 Human OCT2 Transporter Cells
GM1006 Human OATP1B3 Transporter Cells
GM1008 Human OCT1 Transporter Cells
GM1013 Human NTCP Transporter Cells
GM1014 Human MATE1 Transporter Cells
GM1001 Mock Cells

FreeStyle™ 293-F Cells (Gibco™)

The FreeStyle™ 293-F cell line is derived from the 293 cell line and is intended for use with the FreeStyle™ MAX 293 Expression System or FreeStyle™ 293 Expression System. FreeStyle™ 293-F cells are adapted to suspension culture in FreeStyle™ 293 Expression Medium. Frozen cells are supplied in and may be thawed directly into FreeStyle™ 293 Expression Medium. The FreeStyle™ 293-F cell line is supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in 90% FreeStyle™ 293 Expression Medium and 10% DMSO. Characteristics of FreeStyle™ 293-F cells:

• Prepared from low-passage Master Cell Bank cultures derived from parental 293-F cells that were re-cloned by limiting dilution. The 293 clonal derived cultures are maintained in serum-free conditions for only 30 to 35 total passages.
• Adapted to high-density, serum-free, suspension growth and may be maintained in FreeStyle™ 293 Expression Medium.
• Demonstrate high transfection efficiencies with FreeStyle™ MAX reagent.
• Suspension cultures may be transfected in FreeStyle™ 293 Expression Medium without the need to change media.
• Permits transfection of cells at large volumes.

Flp-In™-BHK Cell Line (Invitrogen™)

Flp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. Targeted integration of a Flp-In™ expression vector ensures high-level expression of your gene of interest. There are six Flp-In™ Cell Lines available for the generation of isogenic stable cell lines and a Flp-In™ T-REx™ Cell Line for the generation of tetracycline-regulated stable cell lines. Flp-In™-CV-1, Flp-In™-293, Flp-In™-BHK, Flp-In™-Jurkat, and Flp-In™-3T3 Cell Lines were created by transfecting the parent cell lines with pFRT/lacZeo and selecting for stable Zeocin™- resistant clones. The Flp-In™-CHO Cell Line was created by transfecting CHO cells with pFRT/lacZeo2 and selecting for Zeocin™-resistant clones. The Flp-In™ T-REx™-293 Cell Line contains pFRT/lacZeo and pcDNA™6/TR (from the T-REx™ System) stably integrated. Co-transfection of the Flp-In™ Cell Lines with a Flp-In™ expression vector and the Flp recombinase vector, pOG44, results in targeted integration of the expression vector to the same locus in every cell, ensuring homogeneous levels of gene expression.

Choosing your Flp-In™ Vector/Cell Line Combination
The Flp-In™-CV-1, Flp-In™-293, Flp-In™-CHO, and Flp-In™-Jurkat Cell Lines (Figure 1) work well with Flp-In™ vectors that express a gene from the CMV promoter (pcDNA™5/FRT, pcDNA5/FRT/V5-His-TOPO®, and pSecTag/FRT/V5-His-TOPO®). Flp-In™-BHK and Flp-In™-3T3 cells tend to down regulate the CMV promoter. Therefore, it is recommended that the Flp-In™ vectors containing the EF-1α promoter (pEF5/FRT/V5-DEST™ and pEF5/FRT/V5-D-TOPO®) be used with these cell lines.

FreeStyle™ CHO-S Cells (Gibco™)

FreeStyle™ CHO-S Cells are part of the FreeStyle™ MAX Expression System, which is a breakthrough technology for rapid and high-yield mammalian protein production. This cell line is adapted to high density, serum-freesuspension culture in FreeStyle™ CHO Expression Medium and iscapable of producing high levels of recombinant protein.

Human OATP1B1 SLC Transporter Cells (Gibco™)

TRANSiPORT Human OATP1B1 SLC Transporter Cells are HEK293 cells that transiently overexpress the OATP1B1 solute carrier (SLC) transport protein. Solute carriers are a super-family of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes in and out of cells. The OATP1B1 SLC transporter protein is found in hepatocytes.

• Assess potential for transporter-mediated drug metabolism
• Easy to use format
• Obtain high quality results with a large signal-to-noise ratio

Choice of measurement system
Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.

Assay reliability
Mock HEK293 cells (Cat. No. GM1001) that do not overexpress the SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of transport due to the presence of endogenous transporters or non-specific binding.

Rapid results
The convenient product format enables data generation in 2 days from thawing of the cells to final results.

Related products
GM1003 Human OAT1 Transporter Cells
GM1004 Human OAT3 Transporter Cells
GM1005 Human OCT2 Transporter Cells
GM1006 Human OATP1B3 Transporter Cells
GM1008 Human OCT1 Transporter Cells
GM1013 Human NTCP Transporter Cells
GM1014 Human MATE1 Transporter Cells
GM1015 Human MATE2K Transporter Cells
GM1001 Mock Cells

T-REx™-CHO Cell Line (Invitrogen™)

T-REx™ Cell Lines stably express the tetracycline repressor protein (Table 1). They save significant time and effort when using the T-REx™ System. The T-REx™ Cell Lines are functionally tested by transient transfection with the positive control vector pcDNA™4⁄TO⁄lacZ. T-REx™ Cell Lines exhibit extremely low basal expression levels in the repressed state and high expression upon induction with tetracycline (Figure 1).

Human OATP1B3 SLC Transporter Cells (Gibco™)

TRANSiPORT Human OATP1B3 SLC Transporter Cells are HEK293 cells that transiently overexpress the OATP1B3 solute carrier (SLC) transport protein. Solute carriers are a super-family of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes in and out of cells. The OATP1B3 SLC transporter protein is found in sinusoidal hepatocytes.

• Assess potential for transporter-mediated drug metabolism
• Easy to use format
• Obtain high quality results with a large signal-to-noise ratio

Choice of measurement system
Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.

Assay reliability
Mock HEK293 cells (Cat. No. GM1001) that do not overexpress the SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of transport due to the presence of endogenous transporters or non-specific binding.

Rapid results
The convenient product format enables data generation in 2 days from thawing of the cells to final results.

Related products
GM1002 Human OATP1B1 Transporter Cells
GM1003 Human OAT1 Transporter Cells
GM1004 Human OAT3 Transporter Cells
GM1005 Human OCT2 Transporter Cells
GM1008 Human OCT1 Transporter Cells
GM1013 Human NTCP Transporter Cells
GM1014 Human MATE1 Transporter Cells
GM1015 Human MATE2K Transporter Cells
GM1001 Mock Cells

Human OATP1A2 SLC Transporter Cells (Gibco™)

TRANSiPORT Human OATP1A2 SLC Transporter Cells are HEK293 cells that transiently overexpress the OATP2B1 solute carrier (SLC) transport protein. Solute carriers are a super-family of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes in and out of cells. The OATP1A2 SLC transporter protein is found in hepatocytes.

• Assess potential for transporter-mediated drug metabolism
• Easy-to-use format
• Obtain high-quality results with a large signal-to-noise ratio

Choice of measurement system
Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.

Assay reliability
Mock OATP1A2 SLC Transporter Cells (Cat. No. GM1017) that do not overexpress the OATP1A2 SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of transport due to the presence of endogenous transporters or non-specific binding.

Rapid results
The convenient product format enables data generation in two days from thawing of the cells to final results.

293 F Cells, in CD 293 (Gibco™)

Gibco® 293-F cells were cloned from the original 293 cell line and adapted to Gibco® CD 293 Medium. The 293 cell line is a permanent line established from primary embryonic human kidney and transformed with sheared human adenovirus type 5 DNA. The E1A adenovirus gene is expressed in these cells and participates in transactivation of some viral promoters, allowing these cells to produce very high levels of protein. Gibco® 293-F cells in Gibco® CD 293 Medium feature:
• Quick thaw into suspension culture directly in Gibco® CD 293 Medium or Gibco® 293 SFM II
• Faster growth, superior transfection efficiencies and high protein expression levels
• Documented lineage from a low passage Master Cell Bank
• Quality and performance testing

Quick thaw into suspension culture directly in Gibco® CD 293 Medium or Gibco® 293 SFM II
Each vial containing 7.5 × 106 cells can be thawed directly into suspension culture in either Gibco® CD 293 Medium or Gibco® 293 SFM II supplemented with 4 mM L-glutamine or GlutaMAX™ reagent. Protocols are provided in the product manual.

Faster growth, superior transfection efficiencies and high protein expression levels
Gibco® 293-F cells were prepared from a clone selected for fast growth in serum-free medium, superior transfection efficiencies and a high level of protein expression. Optimal transfection efficiencies are obtained with Lipofectamine™ 2000 or 293fectin™ transfection reagents.

Documented lineage from a low passage Master Cell Bank
The parental 293 cells were cloned by limiting dilution; the clone selected exhibited fast growth and high transfection efficiency. This clone was then re-cloned by limiting dilution; of these clones, the 293-F clone was then selected for its fast growth in serum free medium. The serum-free Master Cell Banks were prepared at approximately passage 25.

Quality and performance testing
Each lot of Gibco® 293-F cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme and karyotype analysis.

Product Use
For Research Use Only. Not for any animal or human therapeutic or diagnostic use. Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.

CHO-S Cells (cGMP banked) and Media Kit (Gibco™)

GIBCO® CHO_S Cells (cGMP Banked) and Media Kit have been developed for the growth of Chinese Hamster Ovary (CHO) cells and expression of recombinant proteins in suspension culture. Parental CHO-S cells have been produced, banked and tested to meet cGMP quality standards. The cells have been adapted to CD CHO Medium for serum free suspension growth. The CD CHO Medium is made from animal origin free and chemically defined components. It contains no proteins, hydrolysates, or components of unknown composition. The medium is formulated without L-glutamine for greater stability, and without phenol red to minimize potential for estrogen-like effects.