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293 H Cells, in CD 293 Gibco™

Gibco® 293-H cells were cloned from the original 293 cell line and adapted to Gibco® CD 293 Medium. The 293 cell line is a permanent line established from primary embryonic human kidney and transformed with sheared human adenovirus type 5 DNA. The E1A adenovirus gene is expressed in these cells and participates in transactivation of some viral promoters, allowing these cells to produce very high levels of protein. Gibco® 293-H cells in Gibco® CD 293 Medium feature:
• Quick thaw into suspension culture directly in Gibco® CD 293 Medium or Gibco® 293 SFM II
• Faster growth, good adherence, superior transfection efficiencies and high protein expression levels
• Documented lineage from a low passage Master Cell Bank
• Quality and performance testing

Quick thaw into suspension culture directly in Gibco® CD 293 Medium or Gibco® 293 SFM II
Each vial containing 7.5 × 106 cells can be thawed directly into suspension culture in either Gibco® CD 293 Medium or Gibco® 293 SFM II supplemented with 4 mM L-glutamine or GlutaMAX™ reagent. Protocols are provided in the product manual.

Faster growth, good adherence, superior transfection efficiencies and high protein expression levels
Gibco® 293-H cells were prepared from a clone selected for fast growth in serum-free medium, good adherence during plaque assays, superior transfection efficiencies and a high level of protein expression. Optimal transfection efficiencies are obtained with Lipofectamine™ 2000 or 293fectin™ transfection reagents.

Documented lineage from a low passage Master Cell Bank
The parental 293 cells were cloned by limiting dilution; the clone selected exhibited fast growth and high transfection efficiency. This clone was then re-cloned by limiting dilution; of these clones, the 293-H clone was then selected for its good adherence during plaque assays. The serum-free Master Cell Banks were prepared at approximately passage 25.

Quality and performance testing
Each lot of Gibco® 293 H cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme and karyotype analysis.

Product Use
For Research Use Only. Not for any animal or human therapeutic or diagnostic use. Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.

293FT Cell Line Invitrogen™

The 293FT Cell line is ideal for generating high-titer lentivirus using the ViraPower™ Lentiviral Expression System. The 293FT Cell Line is a fast-growing, highly transfectable clonal isolate derived from human embryonal kidney cells transformed with the SV40 large T antigen. When a ViraPower™ expression vector and the ViraPower™ Lentiviral Packaging Mix are co-transfected into 293FT cells, high levels of the viral RNA and the gag⁄pol and rev proteins required for packaging are produced.

Advantages
• Fast growing
• Highly transfectable
• Produces high viral titers
• Allows very high levels of protein

Key Features
• Presence of the SV40 large T antigen allows very high levels of protein to be expressed from vectors containing the SV40 origin. Higher, in fact, than other 293 cells or the T antigen-transformed COS cells classically used.

Kit includes
• 293FT Cell Line provided as a frozen stock in 1 ml of 90% complete media and 10% DMSO

Human MATE2K SLC Transporter Cells Gibco™

TRANSiPORT Human MATE2K SLC Transporter Cells are HEK293 cells that transiently overexpress the MATE2K solute carrier (SLC) transport protein. Solute carriers are a super-family of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes in and out of cells. The MATE2K SLC transporter protein is found in kidney proximal tubule cells.

• Assess potential for transporter-mediated drug metabolism
• Easy to use format
• Obtain high quality results with a large signal-to-noise ratio

Choice of measurement system
Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.

Assay reliability
Mock HEK293 cells (Cat. No. GM1001) that do not overexpress the SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of transport due to the presence of endogenous transporters or non-specific binding.

Rapid results
The convenient product format enables data generation in 2 days from thawing of the cells to final results.

Related products
GM1002 Human OATP1B1 Transporter Cells
GM1003 Human OAT1 Transporter Cells
GM1004 Human OAT3 Transporter Cells
GM1005 Human OCT2 Transporter Cells
GM1006 Human OATP1B3 Transporter Cells
GM1008 Human OCT1 Transporter Cells
GM1013 Human NTCP Transporter Cells
GM1014 Human MATE1 Transporter Cells
GM1001 Mock Cells

Flp-In™ T-REx™ 293 Cell Line Invitrogen™

Flp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that ensure homogenous expression of your protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. Targeted integration of a Flp-In™ expression vector ensures high-level expression of your gene of interest. The Flp-In™ T-REx™-293 Cell Line contains pFRT⁄lacZeo and pcDNA™6⁄TR (from the T-REx™ System) stably integrated. Co-transfection of the Flp-In™ Cell Lines with a Flp-In™ expression vector and the Flp recombinase vector, pOG44, results in targeted integration of the expression vector to the same locus in every cell, ensuring homogeneous levels of gene expression.

The Flp-In™-293 work well with Flp-In™ vectors that express a gene from the CMV promoter (e.g., pcDNA™5⁄FRT, pcDNA5⁄FRT⁄V5-His-TOPO®, and pSecTag⁄FRT⁄V5-His-TOPO®).

Human OCT1 SLC Transporter Cells Gibco™

TRANSiPORT Human OCT1 SLC Transporter Cells are HEK293 cells that transiently overexpress the OCT1 solute carrier (SLC) transport protein. Solute carriers are a super-family of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes in and out of cells. The OCT1 SLC transporter protein is found in hepatocytes.

• Assess potential for transporter-mediated drug metabolism
• Easy to use format
• Obtain high quality results with a large signal-to-noise ratio

Choice of measurement system
Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.

Assay reliability
Mock HEK293 cells (Cat. No. GM1001) that do not overexpress the SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of transport due to the presence of endogenous transporters or non-specific binding.

Rapid results
The convenient product format enables data generation in 2 days from thawing of the cells to final results.

Related products
GM1002 Human OATP1B1 Transporter Cells
GM1003 Human OAT1 Transporter Cells
GM1004 Human OAT3 Transporter Cells
GM1005 Human OCT2 Transporter Cells
GM1006 Human OATP1B3 Transporter Cells
GM1013 Human NTCP Transporter Cells
GM1014 Human MATE1 Transporter Cells
GM1015 Human MATE2K Transporter Cells
GM1001 Mock Cells

Human OATP1A2 SLC Transporter Cells Gibco™

TRANSiPORT Human OATP1A2 SLC Transporter Cells are HEK293 cells that transiently overexpress the OATP2B1 solute carrier (SLC) transport protein. Solute carriers are a super-family of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes in and out of cells. The OATP1A2 SLC transporter protein is found in hepatocytes.

• Assess potential for transporter-mediated drug metabolism
• Easy-to-use format
• Obtain high-quality results with a large signal-to-noise ratio

Choice of measurement system
Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.

Assay reliability
Mock OATP1A2 SLC Transporter Cells (Cat. No. GM1017) that do not overexpress the OATP1A2 SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of transport due to the presence of endogenous transporters or non-specific binding.

Rapid results
The convenient product format enables data generation in two days from thawing of the cells to final results.

FreeStyle™ 293-F Cells Gibco™

The FreeStyle™ 293-F cell line is derived from the 293 cell line and is intended for use with the FreeStyle™ MAX 293 Expression System or FreeStyle™ 293 Expression System. FreeStyle™ 293-F cells are adapted to suspension culture in FreeStyle™ 293 Expression Medium. Frozen cells are supplied in and may be thawed directly into FreeStyle™ 293 Expression Medium. The FreeStyle™ 293-F cell line is supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in 90% FreeStyle™ 293 Expression Medium and 10% DMSO. Characteristics of FreeStyle™ 293-F cells:

• Prepared from low-passage Master Cell Bank cultures derived from parental 293-F cells that were re-cloned by limiting dilution. The 293 clonal derived cultures are maintained in serum-free conditions for only 30 to 35 total passages.
• Adapted to high-density, serum-free, suspension growth and may be maintained in FreeStyle™ 293 Expression Medium.
• Demonstrate high transfection efficiencies with FreeStyle™ MAX reagent.
• Suspension cultures may be transfected in FreeStyle™ 293 Expression Medium without the need to change media.
• Permits transfection of cells at large volumes.

Flp-In™-293 Cell Line Invitrogen™

Flp-In™ 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. Targeted integration of a Flp-In™ expression vector ensures high-level expression of your gene of interest. Flp-In™-293 cell line was created by transfecting the parent cell lines with pFRT⁄lacZeo and selecting for stable Zeocin™- resistant clones.

Flp-In™-293 cells work well with Flp-In™ vectors that express a gene from the CMV promoter (e.g., pcDNA™5⁄FRT, pcDNA5⁄FRT⁄V5-His-TOPO®, and pSecTag⁄FRT⁄V5-His-TOPO

Human NTCP SLC Transporter Cells Gibco™

TRANSiPORT Human NTCP SLC Transporter Cells are HEK293 cells that transiently overexpress the NTCP solute carrier (SLC) transport protein. Solute carriers are a super-family of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes in and out of cells. The NTCP SLC transporter protein is found in hepatocytes.

• Assess potential for transporter-mediated drug metabolism
• Easy to use format
• Obtain high quality results with a large signal-to-noise ratio

Choice of measurement system
Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.

Assay reliability
Mock HEK293 cells (Cat. No. GM1001) that do not overexpress the SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of transport due to the presence of endogenous transporters or non-specific binding.

Rapid results
The convenient product format enables data generation in 2 days from thawing of the cells to final results.

Related products
GM1002 Human OATP1B1 Transporter Cells
GM1003 Human OAT1 Transporter Cells
GM1004 Human OAT3 Transporter Cells
GM1005 Human OCT2 Transporter Cells
GM1006 Human OATP1B3 Transporter Cells
GM1008 Human OCT1 Transporter Cells
GM1014 Human MATE1 Transporter Cells
GM1015 Human MATE2K Transporter Cells
GM1001 Mock Cells

293A Cell Line Invitrogen™

The 293A Cell Line is a subclone of the 293 Cell Line and has a relatively flat morphology. It facilitates the initial production, amplification and titering of replication-incompetent adenovirus. The cell line contains a stably integrated copy of the E1 gene that supplies the E1 proteins (E1a and E1b) required to generate recombinant adenovirus. The flat morphology of the cells makes the titering procedure easier.

Advantages
• Has flat cell morphology suitable for screening adenoviral constructs

Key Features
• Contains a stably integrated copy of the E1 gene that supplies the E1 proteins (E1a and E1b) required to generate recombinant adenovirus.

Kit includes
• 293A Cell Line provided as a frozen stock in 1 ml of 90% complete media and 10% DMSO

Expi293F™ Cells Gibco™

Expi293F human cells are derived from the 293 cell line, and are a core component of the Expi293 Expression System. They are maintained in suspension culture and will grow to high density in Expi293 Expression Medium. Expi293F cells are highly transfectable and generate superior protein yields compared to standard 293 cell lines in transient protein expression.

Expi293F Cells attributes include:
Suspension culture cells pre-adapted to Expi293 Expression Medium
Demonstrated higher protein expression levels than other 293 cells adapted to Expi293 Expression Medium
Exhibit more rapid cell growth and higher culture viabilities than standard suspension-adapted 293 cells
Consistent transient expression performance of Expi293F cells for over 40 passages beyond thaw
• Also available from a cGMP bank (Cat. No. 100044202)

Frozen cells are supplied in a vial containing 1 ml of cells at 1 x 107 viable cells/ml in Expi293 Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293 Expression Medium. The six-vial multipack eliminates the need to expand and rebank the cells in the lab.

Cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of <1 mL in multiwell plates to >10 L in bioreactors. Protein harvest can be performed at 2 to 7 days post-transfection, depending on the protein expressed. For expression of recombinant antibodies, harvest is typically at 5 to 7 days post-transfection. There is no need for culture medium supplementation or batch feeding during expression.

Human OATP1B3 SLC Transporter Cells Gibco™

TRANSiPORT Human OATP1B3 SLC Transporter Cells are HEK293 cells that transiently overexpress the OATP1B3 solute carrier (SLC) transport protein. Solute carriers are a super-family of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes in and out of cells. The OATP1B3 SLC transporter protein is found in sinusoidal hepatocytes.

• Assess potential for transporter-mediated drug metabolism
• Easy to use format
• Obtain high quality results with a large signal-to-noise ratio

Choice of measurement system
Cell-based assays can be performed using radioisotope-labeled compounds, fluorescence-labeled compounds, or non-labeled compounds. The amount of substrate transported into the cells can be measured directly using a liquid scintillation counter, fluorescence plate reader, or LC-MS/MS, thereby allowing direct evaluation of SLC transporter activity.

Assay reliability
Mock HEK293 cells (Cat. No. GM1001) that do not overexpress the SLC transporter are available for use as a negative experimental control. Some compounds may demonstrate high background levels of transport due to the presence of endogenous transporters or non-specific binding.

Rapid results
The convenient product format enables data generation in 2 days from thawing of the cells to final results.

Related products
GM1002 Human OATP1B1 Transporter Cells
GM1003 Human OAT1 Transporter Cells
GM1004 Human OAT3 Transporter Cells
GM1005 Human OCT2 Transporter Cells
GM1008 Human OCT1 Transporter Cells
GM1013 Human NTCP Transporter Cells
GM1014 Human MATE1 Transporter Cells
GM1015 Human MATE2K Transporter Cells
GM1001 Mock Cells

Viral Production Cells Gibco™

Gibco Viral Production Cells are a clonal derivative of the HEK 293F cell line, and are a core component of the LV-MAX Lentiviral Production System. Viral Production Cells have been adapted to a special chemically-defined, serum-free and protein-free LV-MAX Production Medium. They are maintained in suspension culture and will grow to high density in LV-MAX Production Medium. Viral Production Cells are highly transfectable by LV-MAX transfection reagent and generate superior lentiviral production compared to polyethyleneimine (PEI)-based lentiviral production methods.

• Suspension culture cells facilitate high-throughput screening or scale-up
• Higher viral production levels than other suspension HEK 293 cells
• Rapid cell growth and high culture viabilities

Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in LV-MAX Production Medium and 10% DMSO. The cells should be thawed directly into LV-MAX Production Medium.

The cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable in volume from 96-deep well blocks to 2-L flasks and bioreactors. Lentiviral vector harvest is typically performed at two days post-transfection.

Expi293F™ Cells (cGMP banked) Gibco™

Expi293F Cells are derived from the human 293 cell line, and are a core component of the Expi293 Expression System. They are maintained in suspension culture and will grow to high density in Expi293 Expression Medium. Expi293F Cells are highly transfectable and achieve higher protein yields per cell compared to standard 293 cell lines resulting in transient protein expression yields of up to one gram per liter.

• For product ordering information, please contact us at outlicensing@thermofisher.com
• cGMP-banked suspension culture cells, pre-adapted to Expi293 Expression Medium
• Derived from the same Master Seed Bank as our standard Expi293F Cells (Cat No. A14527)
• Fully documented cGMP-banked cell line
• A component of the fully integrated, animal origin-free Expi293 Expression System for applications requiring cGMP-banked cells
• A Trial Licensing Program allows you or your CMO/CRO provider to purchase one vial of cells for research use with a simple, one-time payment, enabling full evaluation before committing to a commercial license
• Flexible commercial licensing options without royalties

Frozen cells are supplied in a vial containing 1.1 mL of cells at 1 x 107 viable cells/mL in Expi293 Expression Medium and 10% DMSO. Cells should be thawed directly into Expi293 Expression Medium.

Fully documented cGMP cell line
After execution and payment for a Commercial Production License, a Cell Line Documentation Package (>200 pages and specific to the lot of cells purchased) is provided that includes the entire lineage history of the cells starting at receipt of the initial vial at Thermo Fisher Scientific through delivery to the purchaser, as well as all testing reports.

Commercial licensing that is simple, flexible, and without royalties
Our Trial Licensing Program allows you or your CMO/CRO provider to obtain cGMP-banked Expi293F Cells for research use only with a one-time payment. As you move towards commercial use, a flexible Commercial Production or Service License structure is available without the burden of royalties, keeping it simple as a one-time payment. For more information regarding our Trial Licensing Program and commercial licensing, email us at outlicensing@thermofisher.com.

Mock OATP1A2 SLC Transporter Cells Gibco™

These Mock OATP1A2 SLC Transporter Cells are intended for use as a negative control in experiments utilizing Human OATP1A2 SLC Transporter Cells (Cat. No. GM1016). These mock cells do not overexpress the SLC OATP1A2 transport protein and so can be used to determine levels of endogenous transport activity as well as non-specific binding of compounds being tested.
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