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Drosophila S2 Cells in Schneider's Medium (Gibco™)

Gibco® Drosophila S2 cells are used for heterologous protein expression in the DrosophilaM Expression System (DES®). The S2 cell line was derived from a primary culture of late stage (20-24 hours old) Drosophila melanogaster embryos. Many features of the S2 cell line suggest that it is derived from a macrophage-like lineage. S2 cells grow at room temperature in ambient CO2 as a loose, semi-adherent monolayer in tissue culture flasks and in suspension in spinners and shake flasks. Drosophila S2 cells (frozen in Gibco® Schneider's Medium) feature:
• Expression of protein transiently or through generation of stable cell lines
• Quality and performance testing

Expression of protein transiently or through generation of stable cell lines
Drosophila S2 cells can be transfected with the recombinant expression vector alone for transient expression studies or in combination with a selection vector (e.g. pCoHygro or pCoBlast) to generate stable cell lines. A variety of DES® kits are available to allow expression of the recombinant protein of interest in S2 cells.

Quality and performance testing
Each lot of Gibco® S2 cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme and karyotype analysis.

Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.

Mimic™ Sf9 Insect Cells (Gibco™)

Mimic™ Sf9 Insect Cells are a derivative of the Sf9 insect cell line. These cells have been modified to stably express a variety of mammalian glycosyltransferases. Typically, insect cells are unable to process N-glycans to the extent that mammalian cells do. This can affect protein structure, function, antigenicity, and enzymatic activity. The addition of mammalian glycosyltransferases to the Mimic™ Sf9 Insect Cells allows for production of biantennary, terminally sialyated N-glycans from insect cells (1). The cells can be used to produce more mammalian-like proteins in both baculovirus and stable insect expression systems.

1 ml of Mimic™ Sf9 Insect Cells are provided frozen in 60% complete TNM-FH, 30% FBS, 10% DMSO at a concentration of 107 cells/ml. Store frozen Mimic™ Sf9 Insect Cells in liquid nitrogen. Cells are guaranteed stable for 6 months when properly stored.

Sf21 cells in Sf-900™ III SFM (Gibco™)

Gibco® Sf21 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. Gibco® Sf21 cells are adapted to serum-free suspension culture in Gibco® Sf-900™ III SFM, which saves significant time and expense associated with the adaptation of cultures. Gibco® Sf21 cells (frozen in Gibco® Sf-900™ III SFM) feature:
• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Quality and performance testing

Recombinant protein expression from a variety of expression systems
High levels of protein expression in Sf21 cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.

Good growth in adherent or suspension culture
Each vial containing 1 × 107 cells can be thawed directly into suspension or adherent culture. Protocols for adherent and suspension growth in Gibco® Sf-900™ III SFM are available in the product manual. Cultures can easily be transferred between the two conditions for ease of workflow but we recommend the stock cultures are maintained in suspension.

Quality and performance testing
Each lot of Gibco® Sf21 cells is tested for cell growth and viability post-recovery from cryopreservation.

Product Use
For Research Use Only. Not for any animal or human therapeutic or diagnostic use. Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.

Sf21 cells in Sf-900™ II SFM (Gibco™)

Gibco® Sf21 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. Gibco® Sf21 cells are adapted to serum-free suspension culture in Gibco® Sf-900™ II SFM, which saves significant time and expense associated with the adaptation of cultures. Gibco® Sf21 cells (frozen in Gibco® Sf-900™ II SFM) feature:
• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Quality and performance testing

Recombinant protein expression from a variety of expression systems
High levels of protein expression in Sf21 cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.

Good growth in adherent or suspension culture
Each vial containing 1 × 107 cells can be thawed directly into suspension or adherent culture. Protocols for adherent and suspension growth in Gibco® Sf-900™ II SFM are available in the product manual. Cultures can easily be transferred between the two conditions for ease of workflow but we recommend the stock cultures are maintained in suspension.

Quality and performance testing
Each lot of Gibco® Sf21 cells is tested for cell growth and viability post-recovery from cryopreservation.

Product Use
For Research Use Only. Not for any animal or human therapeutic or diagnostic use. Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.

High Five™ Cells in Express Five™ Medium (Gibco™)

High Five™ Cells (BTI-TN-5B1-4) are a clonal isolate derived from the parental Trichoplusia ni cell line (cabbage looper ovary) and are commonly used for the expression of recombinant proteins using the Baculovirus Expression Vector System (BEVS). High Five™ Insect Cells are adapted to serum-free culture in Express Five® SFM for maximum cell growth and recombinant gene expression. High Five™ Insect Cells (frozen in Express Five® SFM) features:

• Recombinant protein expression from a variety of expression systems
• Up to ten-fold increase in secreted protein expression compared to Sf9 cells
• Good growth in adherent or suspension culture
• Quality and performance testing

Recombinant protein expression from a variety of expression systems
High levels of protein expression in High Five™ cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.

Up to ten-fold increase in secreted protein expression compared to Sf9 cells
High Five™ cells are the best choice for expression of secreted protein in insect cells. They consistently express 5–10 times more protein that Sf9 cells.

Good growth in adherent or suspension culture
High Five ™ cells thaw into adherent culture, forming an irregular monolayer with plaques that may be difficult to isolate. Protocols for adherent growth can be found in Express Five® SFM are available in the product manual. High Five™ cells can be adapted to suspension culture.

Quality and performance testing
Each lot of High Five ™ cells is tested for cell growth and viability post-recovery from cryopreservation.

Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.

Sf9 cells in Sf-900™ III SFM (Gibco™)

Gibco® Sf9 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. Gibco® Sf9 cells are adapted to serum-free suspension culture in Gibco® Sf-900™ III SFM, which saves significant time and expense associated with the adaptation of cultures. Gibco® Sf9 cells (frozen in Gibco® Sf-900™ III SFM) feature:
• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Small, regular size that generates even monolayers and plaques
• Documented lineage from a low passage Master Cell Bank
• Quality and performance testing

Recombinant protein expression from a variety of expression systems
High levels of protein expression in Sf9 cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.

Good growth in adherent or suspension culture
Each vial containing 1 × 107 cells can be thawed directly into suspension or adherent culture. Protocols for adherent and suspension growth in Gibco® Sf-900™ III SFM are available in the product manual. Cultures can easily be transferred between the two conditions for ease of workflow but we recommend the stock cultures are maintained in suspension.

Small, regular size that generates even monolayers and plaques
Gibco® Sf9 cells generate a clean, even monolayer and plaques due to their small, round, regular size. Other cells often form more irregular monolayers and plaques.

Documented lineage from a low passage Master Cell Bank
Gibco® Sf9 cells (in Sf-900™ III SFM) were prepared as serum-free, suspension cultures from Sf9 cells that originated at the USDA Insect Pathology Laboratory. The original Sf9 cells were cloned from the parental IPLBSF-21 (Sf21) cell line that was derived from the pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. The serum-free Master Cell Banks were prepared at passage 26.

Quality and performance testing
Each lot of Gibco® Sf9 cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme and karyotype analysis.

Product Use
For Research Use Only. Not for any animal or human therapeutic or diagnostic use. Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.

Sf9 cells in Sf-900™ II SFM (Gibco™)

Gibco® Sf9 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. Gibco® Sf9 cells are adapted to serum-free suspension culture in Gibco® Sf-900™ II SFM, which saves significant time and expense associated with the adaptation of cultures. Gibco® Sf9 cells (frozen in Gibco® Sf-900™ II SFM) feature:
• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Small, regular size that generates even monolayers and plaques
• Documented lineage from a low passage Master Cell Bank
• Quality and performance testing

Recombinant protein expression from a variety of expression systems
High levels of protein expression in Sf9 cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.

Small, regular size that generates even monolayers and plaques
Gibco® Sf9 cells generate a clean, even monolayer and plaques due to their small, round, regular size. Other cells often form more irregular monolayers and plaques.

Documented lineage from a low passage Master Cell Bank
Gibco® Sf9 cells (in Sf-900™ II SFM) were prepared as serum-free, suspension cultures from Sf9 cells that originated at the USDA Insect Pathology Laboratory. The original Sf9 cells were cloned from the parental IPLBSF-21 (Sf21) cell line that was derived from the pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. The serum-free Master Cell Banks were prepared at passage 34.

Quality and performance testing
Each lot of Gibco® Sf9 cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme and karyotype analysis.

Product Use
For Research Use Only. Not for any animal or human therapeutic or diagnostic use. Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.

ExpiSf9™ Cells (Gibco™)

ExpiSf9 Cells are a non-engineered derivative of Sf9 insect cells that have been adapted for high-density suspension growth in ExpiSf CD Medium.

ExpiSf9 Cells:
• Enable consistent protein expression performance for over 20 passages after thaw
• Allow you to attain yields of three times more protein per liter than other insect cells from baculovirus-infected culture as part of the ExpiSf Expression System
• Achieve maximum cell densities of 2x107 cells per mL in ExpiSf CD Medium
• Exhibit fast recovery post-thaw and doubling times of approximately 24 hours
• Achieve high performance growth in multiple culture volumes from 4 mL in deep-well plates to >3 L in shake flasks

ExpiSf9 Cells are a core component of the ExpiSf Expression System. Frozen cells are supplied in a vial containing 1.5 mL of cells at 1 x 107 viable cells per mL in ExpiSf CD Medium and 7.5% DMSO. The cells should be thawed in ExpiSf CD Medium. Cells are typically grown in shake flasks on a shaker platform in a non-humidified, non-CO2 cell culture incubator. Growing ExpiSf9 cells in adherent format for routine culture is not recommended.

Sf9 Cells in Grace's (Gibco™)

Gibco® Sf9 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. The cells originated at the USDA Insect Pathology Laboratory, from the parental IPLBSF-21 (Sf21) cell line, which was derived from the pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. Gibco® Sf9 cells (frozen in Grace's Insect Medium) feature:
• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Small, regular size that generates even monolayers and plaques
• Quality and performance testing

Recombinant protein expression from a variety of expression systems
High levels of protein expression in Sf9 cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.

Good growth in adherent or suspension culture
Protocols for adherent or suspension growth in Gibco® Supplemented Grace's Insect Medium are available in the product manual. Cultures can easily be transferred between the two conditions for ease of workflow.

Small, regular size that generates even monolayers and plaques
Gibco® Sf9 cells generate a clean, even monolayer and plaques due to their small, round, regular size. Other cells often form more irregular monolayers and plaques.

Quality and performance testing
Each lot of Gibco® Sf9 cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme and karyotype analysis.

Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.

Sf21 Cells in Grace's (Gibco™)

Gibco® Sf21 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. The cells originated at the USDA Insect Pathology Laboratory, where they were derived from the pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. Gibco® Sf21 cells (frozen in Grace's Insect Medium) feature:
• Recombinant protein expression from a variety of expression systems
• Good growth in adherent or suspension culture
• Quality and performance testing

Recombinant protein expression from a variety of expression systems
High levels of protein expression in Sf21 cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.

Good growth in adherent or suspension culture
Protocols for adherent or suspension growth in Gibco® Supplemented Grace's Insect Medium are available in the product manual. Cultures can easily be transferred between the two conditions for ease of workflow.

Quality and performance testing
Each lot of Gibco® Sf21 cells is tested for cell growth and viability post-recovery from cryopreservation.

Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.