Shop All Mammalian Expression Kits

ExpiCHO™ Expression System Kit (Gibco™)

The ExpiCHO™ Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells. It is based on high-density culture of ExpiCHO-S™ cells in ExpiCHO™ Expression Medium. Transient expression is powered by ExpiFectamine™ CHO transfection reagent, designed specifically for high-density CHO suspension culture, in combination with a specialized transfection enhancer and culture feed. All components work together synergistically to generate 2- to 10-fold higher protein yields than the HEK293-based Expi293™ Expression System Kit and 20- to 150-fold higher yields than the FreeStyle™ MAX CHO Expression System. Expression yields of 1 to 3 g per L of transfected culture have been demonstrated for some antibody and non-antibody proteins.

The ExpiCHO Expression System offers:

• Milligram to multigram yields of recombinant protein per liter of culture in 5 to 14 days post-transfection, which are frequently higher than transient 293-based systems
• Cost-effective transient expression in CHO cells, allowing scientists developing protein biotherapeutics to work with CHO-expressed proteins from start to finish in the drug development process
• A robust alternative mammalian transient expression host, allowing for production of proteins that are difficult to express in HEK293 cells
• Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows
• Scalable expression for cultures from <1 mL to >10 L, thus generating the amount of protein needed for your application

The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO™ SFM, and an antibody-expressing positive control vector. All components of the ExpiCHO Expression System Kit are also available for purchase separately. All components of the system are animal origin–free.

Expi293™ Inducible Expression System Kit (Gibco™)

The Expi293 Inducible Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells. The system kit is centered on engineered Expi293F cells that stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression. The Expi293F Inducible cell line was established by the stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter. This cell line is a powerful tool for tetracycline-inducible high-yield expression of recombinant proteins.

Expi293 Inducible Expression System Kit features include:
• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• pcDNA5/TO expression vector for tetracycline-regulated expression of the gene of interest
• Suspension, high-density culture of Expi293F Inducible cells in Expi293 Expression Medium
• High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers

The components included in the Expi293 Inducible Expression System Kit are: one vial of frozen Expi293F Inducible cells, 1 L of Expi293 Expression Medium, one ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture, 100 mL Opti-Plex Complexation Buffer, one pcDNA5/TO Mammalian Expression Vector kit, 500 mg tetracycline hydrochloride, and the pRABBIT IgG IRES-EmGFP positive control vector. All components of the Expi293 Inducible Expression System are also available for individual purchase.

Expi293™ Inducible GnTI- Expression System Kit (Gibco™)

The Expi293 Inducible GnTI- Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells. The system kit is centered on engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity, therefore lacking complex N-glycans, and stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression. The Expi293F Inducible GnTI- cell line was established by the selective knockout of the GnTI gene and the stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter, in parental Expi293F cells. This cell line is a powerful tool for tetracycline-inducible high-yield expression of homogeneously glycosylated recombinant proteins.

Expi293 Inducible GnTI- Expression System Kit features include:
• Homogeneous N-glycosylation of expressed proteins
• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• pcDNA5/TO expression vector for tetracycline-regulated expression of the gene of interest
• Suspension, high-density culture of Expi293F Inducible GnTI- cells in Expi293 Expression Medium
• High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers

The components included in the Expi293 Inducible GnTI- Expression System Kit are: one vial of frozen Expi293F Inducible GnTI- cells, 1 L of Expi293 Expression Medium, one ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture, 100 mL Opti-Plex Complexation Buffer, one PNGase F Glycan Cleavage kit, one pcDNA5/TO Mammalian Expression Vector kit, 500 mg tetracycline hydrochloride, and the pRABBIT IgG IRES-EmGFP positive control vector. All components of the Expi293 Inducible GnTI- Expression System are also available for individual purchase.

Freedom™ DG44 Kit (Gibco™)

Gibco® Freedom® DG44 Kit is an easy-to-use, beginning-to-end product, for cloning and expression of recombinant proteins in dihydrofolate reductase deficient (DHFR-) Chinese Hamster Ovary cells. The Gibco® Freedom® DG44 Kit includes components for transfection, expression, clone creation, and stable cell line selection, all in a conveniently packaged kit. The Gibco® Freedom® DG44 Kit also delivers a proven host cell line with simplified commercial licensing options.

• Flexible commercial licensing without the need for royalties
• Proven host used in approved products and a wide array of proteins
• Complete workflow that can take your gene of interest from transfection to lead clone typically in less than 6 months
• Convenient packaging, for simplified ordering and storage

Commercial Licensing that is Simple, Flexible and Without Royalties
Multiple companies provide CHO (Chinese Hamster Ovary) stable cell line development services and products that require annual fees during development of your therapeutic protein. In addition, royalties may be required on the sales of your therapeutic protein upon commercialization. Gibco® Freedom® Kits provide you with the tools that enable you or your CRO provider to develop your stable cell line for just the price of the kit itself. Furthermore, as you move towards commercial use, a flexible license structure is available without the burden of royalties and works to keep it as simple as a onetime payment (Fig 1). For more information regarding licensing, email us at outlicensing@lifetech.com.

Host Cell Background with Proven Results
DG44 cells have been a choice of scientists for decades for the expression of IgG and numerous blood proteins. Protein expression levels can vary significantly depending on the class of protein, however gram per liter levels of IgG expression has been demonstrated using these DG44 parental cells. So you can be confident that if your work calls for use of DG44 cells, they will deliver expected protein levels.

Complete, Fast, and Efficient Workflow
The Gibco® Freedom® DG44 Kit’s complete, proven, and fully integrated workflow allows you to move from transfection to lead clone typically in less then 6 months, helping to reduce hands on time and Fulltime Equivalent (FTE) requirements (Fig 2). We have implemented a single round of MTX (methotrexate) amplification in the protocol, which in our hands provided up to 120 fold titer improvement in less than 6 weeks (Fig 3). The entire workflow is performed in animal origin free (AOF) medium. The kit also offers the following advantages:

• Cloning of one or two genes that encode your protein(s) of interest
• High efficiency transfection of DNA into DG44 Cells (cGMP-banked)
• Included flash drive with loaded manual

New Convenient Packaging
To free you to focus on the creation of your stable cell line rather than on figuring out where and when all the components of your experiment are arriving, Freedom® DG44 Kit introduces the smART™ tote packaging system (shown in above product photos). All components for the creation of your expression cell line are delivered in soft cooler totes along with a parts flyer, and a flash drive containing the manual. Upon delivery you have all the information needed for correct storage and use of components consolidated in the two smART™ tote shipment coolers.

Access to Gibco® Freedom® Kit Support
If any specific support is needed beyond the manual, please contact your account manager to obtain a Process Science Manager consultation. In addition, one of our support scientists may check in with you to ensure that your experience with the kit is as successful as possible. For further assistance, please email our support team at GibcoServices@thermofisher.com.

MembranePro™ Functional Protein Support Kit (Invitrogen™)

For customers studying GPCRs and other membrane proteins, MembranePro™ is the ready-to-use system that delivers enriched, functional membrane proteins efficiently and reliably. Benefits of MembranePro™ include:

• Proteins are displayed on human (or other mammalian) cell membranes
• Cellular quality control and mammalian posttranslational processing helps produce functional protein
• Proteins bud off as lipoparticles and are easily collected from the culture medium
• Lipoparticles are enriched with expressed receptors

MembranePro™ workflow is less labor intensive in comparison to traditional production of membrane fractions. Briefly, the MembranePro™ protocol allows you to:

1. Clone your gene of interest using a TOPO® vector
2. Transfect with Lipofectamine™ 2000 and MembranePro™ Reagent into 293FT cells
3. Harvest lipoparticles 48h post-transfection, concentrate with MembranePro™ Precipitation Mix
4. Store lipoparticles at -80 C or proceed to downstream assays

MembranePro™ products are offered in two configurations – as an expression kit (TOPO® vector, Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent, 293FT cells) and as a support kit (Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent). The expression kit is only available in a 10 reaction size; the support kit is available in 10, 60 and 600 reaction sizes.

FreeStyle™ 293 Expression System (Invitrogen™)

The FreeStyle™ 293 Expression System is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein. Free your lab from the time-consuming, labor-intensive process of seeding, feeding, passing, and maintaining multiple flasks of adherent cells (Figure 1). The FreeStyle™ 293 Expression System combines GIBCO® FreeStyle™ 293 Expression Medium with 293fectin™, a cationic-lipid formulation designed to transfect suspension FreeStyle™ 293-F cells at high efficiency. FreeStyle™ 293-F cells and 293fectin™ are available as part of the Expression System or separately.

FreeStyle™ 293 Expression Medium is chemically-defined, protein-free medium specifically developed for the ability to support the growth and transfection of 293-F cells under suspension type culture conditions (Figure 2). The medium is a complete, ready-to-use medium that has been supplemented with GlutaMAX™-I Supplement and is animal-origin free. FreeStyle™ 293 Expression Medium is able to save significant time and costs associated with adaptation of cell cultures to serum-free conditions.

Jump-In™ GripTite™ HEK 293 Kit

The Jump-In™ GripTite™ HEK293 Kit allows the targeted integration of genetic material into a specific pre-engineered R4 site in the Jump-In™ GripTite™ HEK293 cell line, to create an isogenic stable cell line with less effort and in less time than traditional cell engineering methods.

The high retargeting efficiency, made possible by the R4 sites in the GripTite™ HEK293 cell line, allows the use of the isogenic pool for additional experiments without the need for clonal selection. Alternatively, the high retargeting efficiency allows for the easy selection of a positive stable clone expressing your gene of interest.

The Jump-In™ GripTite™ HEK293 Kit Lets You:

• Quickly and efficiently develop stably engineered isogenic cell pools in about half the time compared to traditional cell engineering methods
• Utilize isogenic expression from a defined genomic locus as the ideal solution for comparative analysis of gene families, isoforms, or orthologs
• Generate multiple cell lines in parallel using the simplified work flow
• Easily access the technology without complicated licenses or restrictions to interpret

Save Time with Rapid and Efficient Generation of Engineered Cell Lines
With the Jump-In™ GripTite™ HEK293 Kit you can generate functional cell pools in as little as 2 weeks without laborious clone isolation and analysis, and the streamlined protocol makes it easier to generate several cell lines at the same time. Even generation of clonal cell lines can be done with reduced time and effort due to the high percentage of positive clones. In addition, the Jump-In™ technology gives you the freedom to generate an unlimited number of cell lines without restrictive licensing requirements.

Expand Your Experimental Capabilities
The Jump-In™GripTite™ HEK293 Kit is the ideal solution for cells and assays where transient engineering technologies are problematic, as well as for difficult to "engineer" cell lines. The kit also provides a convenient way to create target panels of gene families, isoforms, or orthologs. Genes coding for large proteins or multi-unit proteins are not a problem since the Gateway® destination vectors accept large inserts.

The kit includes:
• pJTI™ R4 Dest CMV pA vector (100 µg)
• pJTI™ R4 Int vector (100 µg)
• Jump-In™GripTite™ HEK293 Cells (2 vials @ 1 ml each)

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Expi293™ Expression System Kit (Gibco™)

The Expi293 Expression System is a complete solution for rapid, high-yield protein production from mammalian 293 cells. It is based on integrated components including high-density Expi293F cells in Expi293 Expression Medium and the cationic lipid-based ExpiFectamine 293 transfection reagent in combination with specialized transfection enhancers.

The Expi293 Expression System features:
• Up to 1 g/L of protein; 2- to 10-fold higher protein yields than previous generation transient expression systems such as the FreeStyle 293 Expression System
• Rapid production of recombinant proteins in 5–7 days
• High density cultures of Expi293F cells that result in more expressing cells per milliliter of culture
• Native folding and mammalian post-translational modifications of expressed proteins
• Easy, robust culture and transfection protocols
• Scalable expression from <1 mL to >10 L

The components included in the Expi293 Expression System Kit are: two vials of frozen Expi293F cells, one liter of Expi293 Expression Medium, one ExpiFectamine 293 Transfection kit sufficient to transfect 1 liter of culture, Opti-MEM I Reduced Serum Medium, and an antibody-expressing positive control vector. All components of the Expi293 Expression System are also available for individual purchase.

Engineered Expi293F cell lines for a range of applications are also available for individual purchase or as part of kits: Expi293F GnTI- Cells (Cat. No. A39240). Expi293F Inducible Cells (Cat. No. A39241), and Expi293F Inducible GnTI- Cells (Cat. No. A39242).

Expi293™ MembranePro™ Expression System (Gibco™)

For researchers studying G protein–coupled receptors (GPCRs) and other membrane proteins, the Expi293™ MembranePro™ Expression System delivers enriched, functional membrane proteins with high efficiency, scalability and reliability for a wide variety of low or high throughput applications.

Features of the Expi293™ MembranePro™ Expression System include:
• Efficient—more than 20-fold increase in membrane protein yield compared to the adherent culture system
• Scalable—easy scale-up without the need for multiple flasks
• Easy to use—no enzymatic or mechanical dissociation required

In addition, benefits of MembranePro™ technology include:
• Proteins are displayed on human (or other mammalian) cell membranes
• Cellular quality control and mammalian post-translational processing helps produce functional protein
• Proteins bud off as lipoparticles and are easily collected from the culture medium
• Lipoparticles are enriched with expressed receptors

With the less labor-intensive Expi293™ MembranePro™ Expression System protocol, you will:
1. Clone your gene of interest using a TOPO® vector
2. Transfect a susupension culture of Expi293F™ cells using Expifectamine™ 293 transfection reagent
3. Harvest lipoparticles 48 hrs after addition of enhancers using MembranePro™ Precipitation Mix
4. Store lipoparticles at -80 C or proceed to downstream assays

Note: Exi293F™ Cells (A14527) and a pEF6 V5-His TOPO® Expression Kit (K9610-20) are required for use of this system and must be purchased separately when needed.

FreeStyle™ MAX 293 Expression System (Gibco™)

The FreeStyle MAX System is a complete system for mammalian transient protein production. This system combines Gibco FreeStyle media, FreeStyle MAX Reagent, and either FreeStyle CHO-S cells or FreeStyle 293 cells. The only equipment required to use the FreeStyle MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinner culture.

The FreeStyle MAX System provides:
• An easy, rapid protocol for transient transfection of CHO and 293 cells in suspension cultures
• Up to milligram quantities of functional proteins with the expected post-translational modifications
• Simplified downstream purification of secreted proteins with FreeStyle™ serum-free medium
• Straightforward protocol for large-scale production

The FreeStyle MAX System can replace time-consuming stable cell line generation with large-scale functional protein production in one week.

Expi293™ Met (–) Protein Labeling Kit (Gibco™)

The Expi293 Met (–) Protein Labeling Kit includes Expi293 Met (–) Expression Medium and the ExpiFectamine 293 Met (–) Transfection Kit for rapid, high-yield transient protein expression from Expi293F cells for applications where labeling of methionine residues in proteins is desired. The kit components are specifically formulated without methionine, allowing users to add labeled methionine of their choice.

Expi293 Met (–) Protein Labeling Kit features include:
• Methionine-free medium and transfection enhancer formulations
• 2- to 10-fold higher protein yields compared to other transfection reagents used for high-density 293 cell culture
• Same transient transfection protocol as ExpiFectamine 293 Transfection Kit
• Scalable transfection from small to large scale while maintaining equivalent volumetric protein yields

The Expi293 Met (–) Protein Labeling Kit is specifically designed to easily integrate with other components of the Expi293 Expression System, including Expi293F cells and Expi293 Expression Medium. The components included in the Expi293 Met (–) Protein Labeling Kit are: 1 L Expi293 Met (–) Expression Medium and one ExpiFectamine 293 Met (–) Transfection Kit sufficient to transfect 1 L of culture.

L-methionine (methyl-13C) and L-selenomethionine labeling reagents are available separately.

Jump In™ T-REx™ CHO-K1 Kit

The Jump-In™ T-REx™ CHO-K1 Kit allows the targeted integration of genetic material into a specific pre-engineered R4 site in the Jump-In™ CHO-K1 cell line, to create an inducible isogenic stable cell line with less effort and in less time than traditional cell engineering methods. These cells stably express the tetracycline repressor protein, allowing for inducible expression of your retargeted gene of interest upon the addition of doxycycline.

The high retargeting efficiency, made possible by the R4 sites in the CHO-K1 cell line, allows the use of the isogenic pool for additional experiments without the need for clonal selection. Alternatively, the high retargeting efficiency allows for the easy selection of a positive stable clone expressing your gene of interest.

The Jump-In™ T-REx™ CHO-K1 Kit lets you:

• Quickly and efficiently develop stably engineered isogenic cell pools in about half the time compared to traditional cell engineering methods
• Inducibly express your gene of interest using doxycycline
• Utilize isogenic expression from a defined genomic locus as the ideal solution for comparative analysis of gene families, isoforms, or orthologs
• Generate multiple cell lines in parallel using the simplified work flow
• Easily access the technology without complicated licenses or restrictions to interpret

Save Time with Rapid and Efficient Generation of Engineered Cell Lines
With the Jump-In™ T-REx™ CHO-K1 Kit you can generate functional cell pools in as little as 2 weeks without laborious clone isolation and analysis, and the streamlined workflow makes it easier to generate several cell lines at the same time. Even generation of clonal cell lines can be done with reduced time and effort due to the high percentage of positive clones. In addition, the Jump-In™ technology gives you the freedom to generate an unlimited number of cell lines without restrictive licensing requirements.

Expand Your Experimental Capabilities
The Jump-In™ T-REx™ CHO-K1 Kit allows for inducible expression of your toxic or unstable gene of interest and is the ideal solution for targets and assays where transient engineering technologies are problematic. The kit also provides a convenient way to create target panels of gene families, isoforms, or orthologs. Genes coding for large proteins or multi-unit proteins are not a problem since the Gateway® destination vectors accept large inserts.

The kit includes:

Jump-In™ T-REx™ CHO-K1 Cells (2 vials @ 1 ml each)
pJTI™ R4 Dest CMV TO pA Vector (100 µg)--A Gateway® Destination vector that drives inducible expression of your gene of interest under control of the strong CMV promoter by inclusion of the Tet-Operon.
pJTI™ R4 Int Vector (100 µg)--Expresses the R4 integrase to facilitate the retargeting of your gene of interest into the R4 site.

Sold separately:

pJTI™ R4 CMV-TO MCS pA Vector--A multi-site cloning vector that drives inducible expression of your gene of interest under control of the strong CMV promoter by inclusion of the Tet-Operon.
pJTI™ R4 EXP CMV-TO EmGFP pA Vector--A control vector with inducible expression of GFP under control of the strong CMV promoter by inclusion of the Tet-Operon.

Freedom™ CHO-S™ Kit (Gibco™)

Gibco® Freedom® CHO-S® Kit, co-developed with ProBioGen AG, is an easy-to-use, beginning-to-end product, for cloning and expression of recombinant proteins in Chinese Hamster Ovary (CHO)-derived suspension culture. The Gibco® Freedom® CHO-S® Kit includes components for transfection, expression, clone creation, and stable cell line selection, all in a conveniently packaged kit. The Gibco® Freedom® CHO-S® Kit also delivers high titers, and offers simplified commercial licensing options.

• Flexible commercial licensing without the need for royalties
• Ability to achieve IgG titers over 3g/L
• Complete workflow that can take your gene of interest from transfection to lead clone typically in 5 months
• Convenient packaging, for simplified ordering and storage

Commercial Licensing that is Simple, Flexible and Without Royalties
Multiple companies provide CHO (Chinese Hamster Ovary) stable cell line development services and products that require annual fees during development of your therapeutic protein. In addition, royalties may be required on the sales of your therapeutic protein upon commercialization. Gibco® Freedom® Kits provide you with the tools that enable you or your CRO provider to develop your stable cell line for just the price of the kit itself. Furthermore, as you move towards commercial use, a flexible license structure is available without the burden of royalties and works to keep it as simple as a onetime payment (Fig 1). For more information regarding licensing, email us at outlicensing@lifetech.com.

Industry Standard Production Levels
Current bioprocess industry standards to achieve manufacturing efficiencies for IgG type molecule production with stable CHO cell lines in bioreactors is 2-3g/L or better. We have demonstrated that the Gibco® Freedom® CHO-S® Kit can consistently generate IgG producing scalable clones (stable out to 60 population doublings), that meet this standard of productivity (Fig 2).

Complete, Fast, and Efficient Workflow
The Gibco® Freedom® CHO-S® Kit’s complete, proven, and fully integrated workflow allows you to move from transfection to lead clone typically in 5 months, with reduced hands on time, and reduced Fulltime Equivalent (FTE) requirements (Fig 3). The entire workflow is performed in our CD FortiCHO™ medium, a chemically defined, animal origin free (AOF) medium. The kit also offers the following advantages:

• Cloning of one or two genes that encode your protein(s) of interest
• High efficiency transfection of DNA into CHO-S® Cells (cGMP-banked)
• Included flash drive with loaded manual

New Convenient Packaging
To free you to focus on the creation of your stable cell line rather than on figuring out where and when all the components of your experiment are arriving, Freedom® CHO-S® Kit introduces the smART™ tote packaging system (shown in above product photos). All components for the creation of your expression cell line are delivered in soft cooler totes along with a parts flyer, and a flash drive containing the manual. Upon delivery you have all the information needed for correct storage and use of components consolidated in the two smART™ tote shipment coolers.

Access to Gibco® Freedom® Kit Support
If any specific support is needed beyond the manual, please contact your account manager to obtain a Process Science Manager consultation. In addition, one of our support scientists may check in with you to ensure that your experience with the kit is as successful as possible. For further assistance, please email our support team at GibcoServices@thermofisher.com.

FreeStyle™ MAX CHO Expression System (Gibco™)

The FreeStyle MAX System is a complete system for mammalian transient protein production. This system combines Gibco FreeStyle media, FreeStyle MAX Reagent, and either FreeStyle CHO-S cells or FreeStyle 293 cells. The only equipment required to use the FreeStyle MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinner culture.

The FreeStyle MAX System provides:
• An easy, rapid protocol for transient transfection of CHO and 293 cells in suspension cultures
• Up to milligram quantities of functional proteins with the expected post-translational modifications
• Simplified downstream purification of secreted proteins with FreeStyle™ serum-free medium
• Straightforward protocol for large-scale production

The FreeStyle MAX System can replace time-consuming stable cell line generation with large-scale functional protein production in one week.

Expi293™ GnTI- Expression System Kit (Gibco™)

The Expi293 GnTI- Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells. The system kit is centered on engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity and therefore lack complex N-glycans. The Expi293F GnTI- cell line was established by the selective knockout of the GnTI gene in parental Expi293F cells. This cell line is a powerful tool for the high-yield expression of homogeneously glycosylated recombinant proteins.

Expi293 GnTI- Expression System Kit features include:
• Homogeneous N-glycosylation of expressed proteins
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• Significantly higher protein yields than other HEK293 GnTI- cell line alternatives
• Suspension, high-density culture of Expi293F GnTI- cells in Expi293 Expression Medium
• High-efficiency transient transfection using ExpiFectamine 293 reagent in combination with specialized transfection enhancers

The components included in the Expi293 GnTI- Expression System Kit are: one vial of frozen Expi293F GnTI- cells, 1 L of Expi293 Expression Medium, one ExpiFectamine 293 transfection kit sufficient to transfect 1 L of culture, 100 mL Opti-Plex Complexation Buffer, one PNGase F Glycan Cleavage kit, and the pRABBIT IgG IRES-EmGFP positive control vector. All components of the Expi293 GnTI- Expression System are also available for individual purchase.