Shop All Microspheres

3000 Series Nanosphere™ Size Standards Thermo Scientific™

Conveniently check the sizes of bacteria, viruses, ribosomes and sub-cellular components with Thermo Scientific™ 3000 Series Nanosphere™ Size Standards, excellent for use with any application that requires a NIST™ traceable size standard with a very narrow standard deviation. The 20 to 900nm range of spheres is ideal for the calibration of electron and atomic force microscopes. Standards are also used in laser light scattering studies and colloidal systems research.

Dyed Red Aqueous Fluorescent Particles Thermo Scientific™

Improve sensitivity and detectability for analytical methods with Thermo Scientific™ Fluoro-Max Red Aqueous Fluorescent Particles, microsphere suspensions that are internally dyed and feature bright, high-contrast colors.

4000 Series Monosized Particles Thermo Scientific™

Minimize response of analytic systems to shape effects with Thermo Scientific™ 4000 Series Monosized Particles, excellent for use with any application that requires a NIST™ traceable size standard with a very narrow standard deviation.

5000 Series 0.31μm to 0.93μm Polymer Particle Suspensions Thermo Scientific™

Meet the need for particulate materials with a variety of particle sizes and properties with Thermo Scientific™ 5000 Series Polymer Particle Suspensions, of special interest for research in light scattering, microporous filter checking and aerosol particle generation.

COUNT-CAL™ Count Precision Size Standards Thermo Scientific™

Minimize contamination by eliminating the need for serial dilutions and extensive sample handling with Thermo Scientific™ COUNT-CAL™ Count Precision Size Standards, a cost-effective, convenient and easy to use product for validating liquid particle counters. Packaged in single-use bottles and intended to be sampled directly from the bottle, standards were developed for manufacturers of parenteral drugs and ophthalmic solutions seeking interim verification of USP 788 and 789 (Particulate Matter in Injections and Particulate Matter in Ophthalmic Solutions).

Fluoro-Max Green and Red Dry Fluorescent Particles Thermo Scientific™

Improve sensitivity and detectability for analytical methods with Thermo Scientific™ Fluoro-Max Green and Red Dry Fluorescent Particles, polymer particles that are internally dyed and feature bright, distinct colors.

ChromoSphere™ Dry Dyed Polymer Particles Thermo Scientific™

Achieve high visual contrast with Thermo Scientific™ ChromoSphere™ Dry Dyed Particles, polymer particles that are internally and deeply dyed in intense red or black, resulting in very high contrast and visibility relative to most background materials. Available as dry powders that can be easily suspended in aqueous media, the product line consists of a large assortment of uniform, red or black particle sizes between 50μm and 500μm.

Duke Standards™ 2000 Series Uniform Polymer Particles Thermo Scientific™

Analyze wide size range materials through laser diffraction or other methods with Thermo Scientific™ Duke Standards™ 2000 Series Uniform Polymer Particles, NIST™ traceable polystyrene DVB particles with wider size distributions that mimic real world particles and work better on laser diffraction-type instruments. Particles meet the need for NIST traceable size standards that have slightly wider distributions than our monodisperse 3000/4000 series size standards. The wider distribution provides light scatter across a range of detectors, resulting in a more repeatable measurement.

CML Latex Beads, 4% w/v, 2 µm Invitrogen™

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character, making these ideal materials for the adsorption of materials such as proteins.

These carboxylate modified (CML) latex particles are produced by copolymerizing carboxylic acid containing polymers. The result is a latex polymer particle with a highly charged, relatively hydrophilic and somewhat 'fluffy' surface layer. The CML particles are electrosterically stabilized, and are therefore safe in concentrations of electrolyte up to 1M univalent salt. CML modified latex particles are negatively charged with a surface which has a polyelectrolyte character. It is only when the pH is ~10 that all the carboxyl groups are ionized. This is a hydrophilic latex.

Microspheres Are Available in Many Sizes and Surface Functionalities
25 sizes to choose from—0.02 microns to 16 microns
20 different surface—for ready conjugation to virtually any material
Surfactant free—ultraclean
Various quantities—from milliliter to 500-liter amounts

Key Applications of Microspheres
Instrument calibration (flow cytometry, microscopy, HTS, HCS)
Flow testing (microfluidics, blood flow, water flow, and air flow)
Cell biology tracers (cell differentiation and cell tracing)
Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Get More Information
Read a technical overview of microspheres and find out about high activity beads, super active beads, specialty beads, and review bead-coupling protocols on the IDC Surfactant-free Latex Beads application page.

We’ll Make a Custom Microsphere Product for You
If you don’t see the microsphere you’re looking for in our stocked list, we’ll make a custom microsphere for you. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

7000 Series Copolymer Microsphere Suspensions Thermo Scientific™

Meet the need for particulate materials with a variety of particle sizes and properties with Thermo Scientific™ 7000 Series Copolymer Microsphere Suspensions, useful as model systems for fluid mechanics experiments and as challenge particles for large pore filtration systems.

Ezy-Cal™ Count Precision Size Standards Thermo Scientific™

Meet the unique and demanding requirements of the contamination control laboratory in modern industries with Thermo Scientific™ Ezy-Cal™ Count Precision Size Standards, easy-to-use liquid particle count controls at 2000 particles per mL. Designed for regular use in liquid particle counters to verify instrument calibration before each use, standards assure reproducibility of the particle counter by permitting a continuous record of its performance using a NIST™-traceable microsphere suspension with a precise concentration.

FluoSpheres™ Sulfate Microspheres, 0.02 µm, yellow-green fluorescent (505/515), 2% solids Invitrogen™

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green (505/515)
Nominal bead diameter: 0.02 µm
Coupling surface: Sulfate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

9000 Series Glass Particle Standards Thermo Scientific™

Replace polystyrene spheres in many applications with Thermo Scientific™ 9000 Series Glass Particle Standards, dry glass NIST™-traceable size standards. Glass microspheres can be used in any application that requires a NIST™-traceable size standard with a narrow size distribution where sample conditions may not be suitable for polystyrene spheres. The glass microspheres provide a better tolerance to chemicals and solvents in addition to higher mechanical and thermal stability.

ChromoSphere™-T Certified Size Standards Thermo Scientific™

Achieve high visual contrast with Thermo Scientific™ ChromoSphere™-T Certified Size Standards, colored polymer microspheres with NIST™-traceable diameters. These reference and calibration materials are used in the testing of filtration systems, vial cleaning evaluations, contamination control studies and calibration of injection devices.

Cyto-Plex™ Carboxylated Particles Thermo Scientific™

Save time when measuring analytes with Thermo Scientific™ Cyto-Plex™ Carboxylated Particles, open platform particles for suspension array analysis.
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