Shop All Microspheres

Opti-Link™ Carboxylate-Modified Particles (Thermo Scientific™)

Control sensitivity, specificity and stability with various acid content available within Thermo Scientific™ Opti-Link™ Carboxylate-Modified Particles.

3K/4K Series Particle Counter Standards (Thermo Scientific™)

Ensure accurate and convenient calibration of laser particle counters with Thermo Scientific™ 3K/4K Series Particle Counter Standards.

Opti-Bind™ Polystyrene Sulfate Particles (Thermo Scientific™)

Achieve maximum reactivity in many diagnostic applications with Thermo Scientific™ Opti-Bind™ Polystyrene Sulfate Particles.

CML Latex Beads, 4% w/v, 5 µm (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character, making these ideal materials for the adsorption of materials such as proteins.

These carboxylate modified (CML) latex particles are produced by copolymerizing carboxylic acid containing polymers. The result is a latex polymer particle with a highly charged, relatively hydrophilic and somewhat 'fluffy' surface layer. The CML particles are electrosterically stabilized, and are therefore safe in concentrations of electrolyte up to 1M univalent salt. CML modified latex particles are negatively charged with a surface which has a polyelectrolyte character. It is only when the pH is ~10 that all the carboxyl groups are ionized. This is a hydrophilic latex.

Microspheres Are Available in Many Sizes and Surface Functionalities
25 sizes to choose from—0.02 microns to 16 microns
20 different surface—for ready conjugation to virtually any material
Surfactant free—ultraclean
Various quantities—from milliliter to 500-liter amounts

Key Applications of Microspheres
Instrument calibration (flow cytometry, microscopy, HTS, HCS)
Flow testing (microfluidics, blood flow, water flow, and air flow)
Cell biology tracers (cell differentiation and cell tracing)
Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Get More Information
Read a technical overview of microspheres and find out about high activity beads, super active beads, specialty beads, and review bead-coupling protocols on the IDC Surfactant-free Latex Beads application page.

We’ll Make a Custom Microsphere Product for You
If you don’t see the microsphere you’re looking for in our stocked list, we’ll make a custom microsphere for you. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

3000 Series Nanosphere™ Size Standards (Thermo Scientific™)

Conveniently check the sizes of bacteria, viruses, ribosomes and sub-cellular components with these size standards.

Power-Bind™ Streptavidin Coated Particles (Thermo Scientific™)

Bind a variety of biotinylated ligands, including DNA/RNA, oligos, antibodies and proteins with Thermo Scientific™ Power-Bind Streptavidin Coated Particles.

CML Latex Beads, 4% w/v, 6 µm (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character, making these ideal materials for the adsorption of materials such as proteins.

These carboxylate modified (CML) latex particles are produced by copolymerizing carboxylic acid containing polymers. The result is a latex polymer particle with a highly charged, relatively hydrophilic and somewhat 'fluffy' surface layer. The CML particles are electrosterically stabilized, and are therefore safe in concentrations of electrolyte up to 1M univalent salt. CML modified latex particles are negatively charged with a surface which has a polyelectrolyte character. It is only when the pH is ~10 that all the carboxyl groups are ionized. This is a hydrophilic latex.

Microspheres Are Available in Many Sizes and Surface Functionalities
25 sizes to choose from—0.02 microns to 16 microns
20 different surface—for ready conjugation to virtually any material
Surfactant free—ultraclean
Various quantities—from milliliter to 500-liter amounts

Key Applications of Microspheres
Instrument calibration (flow cytometry, microscopy, HTS, HCS)
Flow testing (microfluidics, blood flow, water flow, and air flow)
Cell biology tracers (cell differentiation and cell tracing)
Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Get More Information
Read a technical overview of microspheres and find out about high activity beads, super active beads, specialty beads, and review bead-coupling protocols on the IDC Surfactant-free Latex Beads application page.

We’ll Make a Custom Microsphere Product for You
If you don’t see the microsphere you’re looking for in our stocked list, we’ll make a custom microsphere for you. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

ChromoSphere™ Dry Dyed Polymer Particles (Thermo Scientific™)

Achieve high visual contrast with polymer particles that are internally and deeply dyed in intense red or black.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.5 µm, yellow-green fluorescent (505/515), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green (505/515)
Nominal bead diameter: 0.5 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

4000 Series Monosized Particles (Thermo Scientific™)

Minimize response of analytic systems to shape effects with monosized particles.

Dyed Red Aqueous Fluorescent Particles (Thermo Scientific™)

Improve sensitivity and detectability for analytical methods with microsphere suspensions that are internally dyed and feature bright, high-contrast colors.

Fluoro-Max Dyed Blue Aqueous Fluorescent Particles (Thermo Scientific™)

Achieve improved sensitivity and detectability in analytical methods with internally dyed microspheres that feature bright, high-contrast colors.

5000 Series 0.31μm to 0.93μm Polymer Particle Suspensions (Thermo Scientific™)

Meet the need for particulate materials with a variety of particle sizes and properties with Thermo Scientific™ 5000 Series Polymer Particle Suspensions

SURF-CAL™ Particle Size Standards (Thermo Scientific™)

Simplify the preparation of calibration wafers with these particle size standards.

Cyto-Plex™ Carboxylated Particles (Thermo Scientific™)

Save time when measuring analytes with Thermo Scientific™ Cyto-Plex™ Carboxylated Particles, open platform particles for suspension array analysis.