Shop All Microspheres

Fluoro-Max Dyed Green Aqueous Fluorescent Particles (Thermo Scientific™)

Improve sensitivity and detectability for analytical methods with green aqueous fluorescent particles.

Opti-Bind™ Polystyrene Sulfate Particles (Thermo Scientific™)

Achieve maximum reactivity in many diagnostic applications with Thermo Scientific™ Opti-Bind™ Polystyrene Sulfate Particles.

Opti-Link™ Carboxylate-Modified Particles (Thermo Scientific™)

Control sensitivity, specificity and stability with various acid content available within Thermo Scientific™ Opti-Link™ Carboxylate-Modified Particles.

Chloromethyl Latex Beads, 4% w/v, 0.4 µm (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character, making these ideal materials for the adsorption of materials such as proteins.

The chloromethyl latex has a high density of chloromethyl groups attached to the styrene monomeric unit. These reactive surface functional groups react directly with amino groups in antibodies, antigens, or other ligands under mild aqueous conditions to yield a stable covalent product by a one-step process. The particles are stabilized by negatively-charged sulfate groups. This type of particle can be used at both high and low pH conditions. This is a hydrophobic latex.

Microspheres Are Available in Many Sizes and Surface Functionalities
25 sizes to choose from—0.02 microns to 16 microns
20 different surface—for ready conjugation to virtually any material
Surfactant free—ultraclean
Various quantities—from milliliter to 500-liter amounts

Key Applications of Microspheres
Instrument calibration (flow cytometry, microscopy, HTS, HCS)
Flow testing (microfluidics, blood flow, water flow, and air flow)
Cell biology tracers (cell differentiation and cell tracing)
Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Get More Information
Read a technical overview of microspheres and find out about high activity beads, super active beads, specialty beads, and review bead-coupling protocols on the IDC Surfactant-free Latex Beads application page.

We’ll Make a Custom Microsphere Product for You
If you don’t see the microsphere you’re looking for in our stocked list, we’ll make a custom microsphere for you. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

Dyed Red Aqueous Fluorescent Particles (Thermo Scientific™)

Improve sensitivity and detectability for analytical methods with microsphere suspensions that are internally dyed and feature bright, high-contrast colors.

4000 Series Monosized Particles (Thermo Scientific™)

Minimize response of analytic systems to shape effects with monosized particles.

FluoSpheres™ NeutrAvidin™-Labeled Microspheres, 0.04 µm, yellow-green fluorescent (505/515), 1% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green fluorescent (505 / 515)
Nominal bead diameter: 0.04 µm
Surface modification: NeutrAvidin®
Solids: 1%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

3K/4K Series Particle Counter Standards (Thermo Scientific™)

Ensure accurate and convenient calibration of laser particle counters with Thermo Scientific™ 3K/4K Series Particle Counter Standards.

Chloromethyl Latex Beads, 4% w/v, 0.6 µm (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character, making these ideal materials for the adsorption of materials such as proteins.

The chloromethyl latex has a high density of chloromethyl groups attached to the styrene monomeric unit. These reactive surface functional groups react directly with amino groups in antibodies, antigens, or other ligands under mild aqueous conditions to yield a stable covalent product by a one-step process. The particles are stabilized by negatively-charged sulfate groups. This type of particle can be used at both high and low pH conditions. This is a hydrophobic latex.

Microspheres Are Available in Many Sizes and Surface Functionalities
25 sizes to choose from—0.02 microns to 16 microns
20 different surface—for ready conjugation to virtually any material
Surfactant free—ultraclean
Various quantities—from milliliter to 500-liter amounts

Key Applications of Microspheres
Instrument calibration (flow cytometry, microscopy, HTS, HCS)
Flow testing (microfluidics, blood flow, water flow, and air flow)
Cell biology tracers (cell differentiation and cell tracing)
Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Get More Information
Read a technical overview of microspheres and find out about high activity beads, super active beads, specialty beads, and review bead-coupling protocols on the IDC Surfactant-free Latex Beads application page.

We’ll Make a Custom Microsphere Product for You
If you don’t see the microsphere you’re looking for in our stocked list, we’ll make a custom microsphere for you. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Polystyrene Microspheres, 15 µm, scarlet fluorescent (645/680), for blood flow determination (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy.

FluoSpheres® microspheres in the 10–15 µm size range were designed specifically for regional blood flow studies in tissues and organs. Typically for these types of studies, the microspheres are injected at desired locations in the circulatory system and eventually lodge in the capillaries, where they can be extracted and the fluorescence quantified. The use of fluorescent microspheres eliminates the radiation hazards and disposal hurdles associated with radiolabeled microspheres. Multiple (up to seven or more with good spectral correction) simultaneously circulating FluoSpheres® microspheres can be discriminated. Microspheres that have been perfused or injected into tissues and organs retain their fluorescence following histological serial sectioning. Our FluoSpheres® microspheres for blood flow determination, which are available in eleven distinguishable fluorescent colors, are also compatible with blood flow analyzer systems that perform automated extraction and analysis.

FluoSpheres® Microsphere Specifications:

Label (Ex/Em): Scarlet fluorescent (645/680)
Nominal bead diameter: 15 µm

Choices for FluoSpheres® Fluorescent Microspheres
In addition to microspheres for blood flow studies, investigate our complete offering of fluorescent microspheres products. Among those products you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

9000 Series Glass Particle Standards (Thermo Scientific™)

Replace polystyrene spheres in many applications with dry glass NIST™-traceable size standards.

3000 Series Nanosphere™ Size Standards (Thermo Scientific™)

Conveniently check the sizes of bacteria, viruses, ribosomes and sub-cellular components with these size standards.

4D Series Dry Microsphere Size Standards (Thermo Scientific™)

Calibrate airborne optical particle counters to comply with ISO 21501-4 with Thermo Scientific™ 4D Series Dry Microsphere Size Standards.

ChromoSphere™-T Certified Size Standards (Thermo Scientific™)

Achieve high visual contrast with Thermo Scientific™ ChromoSphere™-T Certified Size Standards, colored polymer microspheres with NIST™-traceable diameters.

FluoSpheres™ NeutrAvidin™-Labeled Microspheres, 0.2 µm, yellow-green fluorescent (505/515), 1% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green fluorescent (505 / 515)
Nominal bead diameter: 0.2 µm
Surface modification: NeutrAvidin®
Solids: 1%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.