Shop All Microspheres

Color-Rich Dyed Carboxylate-Modified Particles (Thermo Scientific™)

Obtain superior test sensitivity in qualitative and quantitative lateral flow tests with Thermo Scientific™ Color-Rich Dyed Carboxylate-Modified Particles.

Cyto-Plex™ Carboxylated Particles (Thermo Scientific™)

Save time when measuring analytes with Thermo Scientific™ Cyto-Plex™ Carboxylated Particles, open platform particles for suspension array analysis.

Dri-Cal™ Particle Size Standards (Thermo Scientific™)

Use Thermo Scientific™ Dri-Cal™ Particle Size Standards for calibrating particle sizing and counting instruments that require dry particles.

Fluoro-Max Green and Red Dry Fluorescent Particles (Thermo Scientific™)

Improve sensitivity and detectability for analytical methods with fluorescent particles that feature bright, distinct colors.

Flow Cytometry Sub-micron Particle Size Reference Kit (Invitrogen™)

The Flow Cytometry Sub-micron Particle Size Reference Kit provides six sizes of green-fluorescent beads (ranging in diameter from 0.02 µm to 2.0 µm) packaged in individual vials. Each bead is uniform in size, and the green-fluorescent dyes are localized within the bead, which makes the beads brighter and more photostable than conventional surface-stained beads.

These size standards can help identify small bioparticles such as exosomes, bacteria, and viruses in experimental samples. This kit can also be used as a tool to verify instrument performance and establish parameters suitable for analyzing sub-micron particles, including:
• Particle size resolution limit and dynamic range
• Sensitivity of forward and side scatter photomultiplier tubes
• Level of instrument baseline noise
• Laser and optical alignment and stability
• Stability of the fluidics system

The bead diameters are determined by transmission electron microscopy, and the beads are provided as 2 mL suspensions with a density of ~1× 106 beads/mL.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.02 µm, blue fluorescent (365/415), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Blue (365/415)
Nominal bead diameter: 0.02 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

Dyed Red Aqueous Fluorescent Particles (Thermo Scientific™)

Improve sensitivity and detectability for analytical methods with microsphere suspensions that are internally dyed and feature bright, high-contrast colors.

Fluoro-Max Dyed Green Aqueous Fluorescent Particles (Thermo Scientific™)

Improve sensitivity and detectability for analytical methods with green aqueous fluorescent particles.

Sulfate Latex Beads, 8% w/v, 2 µm (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Because of the way that the polystyrene chains arrange themselves in the bead, the surface is very hydrophobic in character, making these ideal materials for the adsorption of materials such as proteins.

These sulfate latex microspheres are stabilized by sulfate charges. In some cases, hydroxyl and carboxyl groups are also present in small amounts bound to the polymer chains. The pKa of the sulfate group is <2 and consequently the particles are stable in acidic media. Suspensions of the particles are stable up to almost 0.30 M univalent electrolyte concentrations and therefore may be used in media of physiological ionic strength. However they are hydrophobic particles and will undergo aggregation in the presence of low concentrations of divalent or trivalent cations unless stabilized by a hydrophilic coating. Sulfate latex is suitable for calibration of particle size analysis equipment and are also appropriate for use in diagnostic test systems which rely upon physical adsorption of antigens or antibodies.

Microspheres Are Available in Many Sizes and Surface Functionalities
25 sizes to choose from—0.02 microns to 16 microns
20 different surface—for ready conjugation to virtually any material
Surfactant free—ultraclean
Various quantities—from milliliter to 500-liter amounts

Key Applications of Microspheres
Instrument calibration (flow cytometry, microscopy, HTS, HCS)
Flow testing (microfluidics, blood flow, water flow, and air flow)
Cell biology tracers (cell differentiation and cell tracing)
Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Get More Information
Read a technical overview of microspheres and find out about high activity beads, super active beads, specialty beads, and review bead-coupling protocols on the IDC Surfactant-free Latex Beads application page.

We’ll Make a Custom Microsphere Product for You
If you don’t see the microsphere you’re looking for in our stocked list, we’ll make a custom microsphere for you. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

5000 Series 0.03μm to 0.30μm Polymer Particle Suspensions (Thermo Scientific™)

Optimize research in light scattering, microporous filter checking, and aerosol particle generation with these particle suspensions.

SURF-CAL™ Particle Size Standards (Thermo Scientific™)

Simplify the preparation of calibration wafers with these particle size standards.

Opti-Link™ Carboxylate-Modified Particles (Thermo Scientific™)

Control sensitivity, specificity and stability with various acid content available within Thermo Scientific™ Opti-Link™ Carboxylate-Modified Particles.

8000 Series Silica Particle Size Standards (Thermo Scientific™)

Provide greater durability and chemical stability when using solvents with these monodisperse silica microspheres used in instrument calibration and as spacers.

Fluoro-Max Dyed Blue Aqueous Fluorescent Particles (Thermo Scientific™)

Achieve improved sensitivity and detectability in analytical methods with internally dyed microspheres that feature bright, high-contrast colors.

5000 Series 1.0μm to 3.2μm Polymer Particle Suspensions (Thermo Scientific™)

Meet the need for particulate materials with a variety of particle sizes and properties.