Shop All Colored & Fluorescent Particles

Fluoro-Max™ Fluorescent Carboxylate-Modified Particles (Thermo Scientific™)

Lower detection limits of fluorescent assays by limiting background interference from relatively short-lived matrix fluorescence.

Cyto-Plex™ Carboxylated Particles (Thermo Scientific™)

Save time when measuring analytes with Thermo Scientific™ Cyto-Plex™ Carboxylated Particles, open platform particles for suspension array analysis.

Fluoro-Max Dyed Green Aqueous Fluorescent Particles (Thermo Scientific™)

Improve sensitivity and detectability for analytical methods with green aqueous fluorescent particles.

Fluoro-Max Green and Red Dry Fluorescent Particles (Thermo Scientific™)

Improve sensitivity and detectability for analytical methods with fluorescent particles that feature bright, distinct colors.

Color-Rich Dyed Carboxylate-Modified Particles (Thermo Scientific™)

Obtain superior test sensitivity in qualitative and quantitative lateral flow tests with Thermo Scientific™ Color-Rich Dyed Carboxylate-Modified Particles.

Dyed Red Aqueous Fluorescent Particles (Thermo Scientific™)

Improve sensitivity and detectability for analytical methods with microsphere suspensions that are internally dyed and feature bright, high-contrast colors.

Fluoro-Max Dyed Blue Aqueous Fluorescent Particles (Thermo Scientific™)

Achieve improved sensitivity and detectability in analytical methods with internally dyed microspheres that feature bright, high-contrast colors.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.02 µm, yellow-green fluorescent (505/515), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green (505/515)
Nominal bead diameter: 0.02 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

ChromoSphere™ Dry Dyed Polymer Particles (Thermo Scientific™)

Achieve high visual contrast with polymer particles that are internally and deeply dyed in intense red or black.

Fluoro-Max Fluorescent Streptavidin-Coated Particles (Thermo Scientific™)

Simplify the binding process with a universal binding reagent.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.04 µm, yellow-green fluorescent (505/515), 5% solids, azide free (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy.

For tracking particles and cells, tracing fluid dynamics, discriminating the size dependence of uptake or transport in vivo, etc., we offer more concentrated, 0.04 µm diameter FluoSpheres® microspheres preparations (5% solids) that lack the sodium azide preservative present in the majority of our FluoSpheres® products. The 0.04 µm particles are small enough to be microinjected or to be taken up by phagocytosis.

We also offer 1.0 µm FluoSpheres® microsphere preparations that contain a much higher dye content than our other FluoSpheres® products. This allows stronger signals to be generated using fewer microspheres per tracing experiment.

In many biological systems, the concentrated fluorescence and spherical shape of the FluoSpheres® beads permit them to be detected against a relatively high but diffuse background fluorescence. Typically for these types of studies, the microspheres and the fluorescent dyes they contain are first extracted from the tissue sample, and then the fluorescence is quantified on a spectrofluorometer or fluorescence microplate reader. The use of fluorescent microspheres eliminates the radiation hazards and disposal hurdles associated with radiolabeled microspheres.

FluoSpheres® Microsphere Specifications:

Label (Ex/Em): Yellow-green fluorescent (505/515)
Nominal bead diameter: 0.04 µm
Coupling surface: Carboxylate
Solids: 5%

Characteristics of the Carboxylate Coupling Surface
Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.

Choices for FluoSpheres® Fluorescent Microspheres
In addition to microspheres for tracing, investigate our complete offering of fluorescent microspheres products. Among those products you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we stand by quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.04 µm, dark red fluorescent (660/680), 5% solids, azide free (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy.

For tracking particles and cells, tracing fluid dynamics, discriminating the size dependence of uptake or transport in vivo, etc., we offer more concentrated, 0.04 µm diameter FluoSpheres® microspheres preparations (5% solids) that lack the sodium azide preservative present in the majority of our FluoSpheres® products. The 0.04 µm particles are small enough to be microinjected or to be taken up by phagocytosis.

We also offer 1.0 µm FluoSpheres® microsphere preparations that contain a much higher dye content than our other FluoSpheres® products. This allows stronger signals to be generated using fewer microspheres per tracing experiment.

In many biological systems, the concentrated fluorescence and spherical shape of the FluoSpheres® beads permit them to be detected against a relatively high but diffuse background fluorescence. Typically for these types of studies, the microspheres and the fluorescent dyes they contain are first extracted from the tissue sample, and then the fluorescence is quantified on a spectrofluorometer or fluorescence microplate reader. The use of fluorescent microspheres eliminates the radiation hazards and disposal hurdles associated with radiolabeled microspheres.

FluoSpheres® Microsphere Specifications:

Label (Ex/Em): Dark red fluorescent (660 / 680)
Nominal bead diameter: 0.04 µm
Coupling surface: Carboxylate
Solids: 5%

Characteristics of the Carboxylate Coupling Surface
Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.

Choices for FluoSpheres® Fluorescent Microspheres
In addition to microspheres for tracing, investigate our complete offering of fluorescent microspheres products. Among those products you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we stand by quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 1.0 µm, red fluorescent (580/605), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Red (580/605)
Nominal bead diameter: 1.0 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 2.0 µm, yellow-green fluorescent (505/515), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green (505/515)
Nominal bead diameter: 2.0 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Size Kit #2, Carboxylate-modified Microspheres, yellow-green fluorescent (505/515), 2% solids, six sizes (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. This FluoSpheres® Size Kit supplies 1 mL of each the 0.02, 0.1, 0.2, 0.5, 1.0, and 2.0 µm sizes.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green fluorescent (505/515)
Nominal bead diameter: 0.02, 0.1, 0.2, 0.5, 1.0, 2.0 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin.
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide.

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.02 µm, Nile Red fluorescent (535/575), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Nile red (535/575)
Nominal bead diameter: 0.02 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

Flow Cytometry Sub-micron Particle Size Reference Kit (Invitrogen™)

The Flow Cytometry Sub-micron Particle Size Reference Kit provides six sizes of green-fluorescent beads (ranging in diameter from 0.02 µm to 2.0 µm) packaged in individual vials. Each bead is uniform in size, and the green-fluorescent dyes are localized within the bead, which makes the beads brighter and more photostable than conventional surface-stained beads.

These size standards can help identify small bioparticles such as exosomes, bacteria, and viruses in experimental samples. This kit can also be used as a tool to verify instrument performance and establish parameters suitable for analyzing sub-micron particles, including:
• Particle size resolution limit and dynamic range
• Sensitivity of forward and side scatter photomultiplier tubes
• Level of instrument baseline noise
• Laser and optical alignment and stability
• Stability of the fluidics system

The bead diameters are determined by transmission electron microscopy, and the beads are provided as 2 mL suspensions with a density of ~1× 106 beads/mL.

FluoSpheres™ Carboxylate-Modified Microspheres, 1.0 µm, Nile Red fluorescent (535/575), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Nile red (535/575)
Nominal bead diameter: 1.0 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

Cyto-Cal™ Alignment Particles (Thermo Scientific™)

Achieve exceptional accuracy and feedback on laser power, alignment and noise in excitation and emission optics with Thermo Scientific™ Cyto-Cal™ Alignment Particles.

FluoSpheres™ Sulfate Microspheres, 4.0 µm, yellow-green fluorescent (505/515), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green (505/515)
Nominal bead diameter: 4.0 µm
Coupling surface: Sulfate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.02 µm, crimson fluorescent (625/645), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Crimson (625/645)
Nominal bead diameter: 0.02 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Sulfate Microspheres, 2.0 µm, yellow-Green fluorescent (505/515), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green (505/515)
Nominal bead diameter: 2.0 µm
Coupling surface: Sulfate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Streptavidin-Labeled Microspheres, 0.04 µm, yellow-green fluorescent (505/515), 0.5% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green fluorescent (505 / 515)
Nominal bead diameter: 0.04 µm
Surface modification: Streptavidin
Solids: 0.5%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ NeutrAvidin™-Labeled Microspheres, 1.0 µm, yellow-green fluorescent (505/515), 1% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green fluorescent (505 / 515)
Nominal bead diameter: 1.0 µm
Surface modification: NeutrAvidin®
Solids: 1%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.1 µm, blue fluorescent (350/440), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Blue (350/440)
Nominal bead diameter: 0.1 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

TransFluoSpheres™ Carboxylate-Modified Microspheres, 1.0 µm (488/645), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® TransFluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with two (or more) fluorescent dyes to produce a microspheres that can be excited by the 488 nm or the 514 nm spectral lines of the argon-ion laser but emit at much longer wavelengths, facilitating multicolor experiments and allowing the use of broadband excitation and emission filters. TransFluoSpheres® microspheres typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy.

TransFluoSpheres® Microsphere Specifications:

Label (Ex/Em): Crimson-fluorescent (488/645)
Nominal bead diameter: 1.0 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Carboxylate Coupling Surface
Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.

Key Applications of Microspheres:
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.1 µm, orange fluorescent (540/560), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Orange (540/560)
Nominal bead diameter: 0.1 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Fluorescent Color Kit, Carboxylate-Modified Microspheres, 0.04 µm, four colors (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. For tracking particles and cells, tracing fluid dynamics, discriminating the size dependence of uptake or transport in vivo, etc., we offer more concentrated, 0.04 µm diameter FluoSpheres® microspheres preparations (5% solids) that lack the sodium azide preservative present in the majority of our FluoSpheres® products. The 0.04 µm particles are small enough to be microinjected or to be taken up by phagocytosis. We also offer 1.0 µm FluoSpheres® microsphere preparations that contain a much higher dye content than our other FluoSpheres® products. This allows stronger signals to be generated using fewer microspheres per tracing experiment. In many biological systems, the concentrated fluorescence and spherical shape of the FluoSpheres® beads permit them to be detected against a relatively high but diffuse background fluorescence. Typically for these types of studies, the microspheres and the fluorescent dyes they contain are first extracted from the tissue sample, and then the fluorescence is quantified on a spectrofluorometer or fluorescence microplate reader. The use of fluorescent microspheres eliminates the radiation hazards and disposal hurdles associated with radiolabeled microspheres. This FluoSpheres® Fluorescent Color Kit supplies 0.04 µm diameter microspheres in 1 mL volumes of each of four fluorescent colors: Yellow-green, orange, red, and dark red.

FluoSpheres® Microsphere Specifications:

Label (Ex/Em): Yellow-green, orange, red, and dark red
Nominal bead diameter: 0.04 µm
Coupling surface: Carboxylate
Solids: 5%

Characteristics of the Carboxylate Coupling Surface
Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.

Choices for FluoSpheres® Fluorescent Microspheres
In addition to microspheres for tracing, investigate our complete offering of fluorescent microspheres products. Among those products you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we stand by quality of our work. We are ISO 9001:2000 certified. For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.04 µm, orange fluorescent (540/560), 5% solids, azide free (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy.

For tracking particles and cells, tracing fluid dynamics, discriminating the size dependence of uptake or transport in vivo, etc., we offer more concentrated, 0.04 µm diameter FluoSpheres® microspheres preparations (5% solids) that lack the sodium azide preservative present in the majority of our FluoSpheres® products. The 0.04 µm particles are small enough to be microinjected or to be taken up by phagocytosis.

We also offer 1.0 µm FluoSpheres® microsphere preparations that contain a much higher dye content than our other FluoSpheres® products. This allows stronger signals to be generated using fewer microspheres per tracing experiment.

In many biological systems, the concentrated fluorescence and spherical shape of the FluoSpheres® beads permit them to be detected against a relatively high but diffuse background fluorescence. Typically for these types of studies, the microspheres and the fluorescent dyes they contain are first extracted from the tissue sample, and then the fluorescence is quantified on a spectrofluorometer or fluorescence microplate reader. The use of fluorescent microspheres eliminates the radiation hazards and disposal hurdles associated with radiolabeled microspheres.

FluoSpheres® Microsphere Specifications:

Label (Ex/Em): orange fluorescent (540 / 560)
Nominal bead diameter: 0.04 µm
Coupling surface: Carboxylate
Solids: 5%

Characteristics of the Carboxylate Coupling Surface
Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.

Choices for FluoSpheres® Fluorescent Microspheres
In addition to microspheres for tracing, investigate our complete offering of fluorescent microspheres products. Among those products you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we stand by quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

Fluoro-Max Green Particulate Marker Particles (Thermo Scientific™)

Effectively mark the “risk zone” in evaluating regional ischemia with Thermo Scientific™ Fluoro-Max Green Particulate Marker Particles.

Prosolia Velox 360™ PaperSpray™ System (Thermo Scientific™)

Analyze biofluid samples directly by mass spectrometry and with minimal sample preparation or liquid chromatography.

FluoSpheres™ Carboxylate-Modified Microspheres, 1.0 µm, yellow-green fluorescent (505/515), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green (505/515)
Nominal bead diameter: 1.0 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.5 µm, yellow-green fluorescent (505/515), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green (505/515)
Nominal bead diameter: 0.5 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Sulfate Microspheres, 0.02 µm, yellow-green fluorescent (505/515), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green (505/515)
Nominal bead diameter: 0.02 µm
Coupling surface: Sulfate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Polystyrene Microspheres, 1.0 µm, red fluorescent (580/605), for tracer studies (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy.

For tracking particles and cells, tracing fluid dynamics, discriminating the size dependence of uptake or transport in vivo, etc., we offer more concentrated, 0.04 µm diameter FluoSpheres® microspheres preparations (5% solids) that lack the sodium azide preservative present in the majority of our FluoSpheres® products. The 0.04 µm particles are small enough to be microinjected or to be taken up by phagocytosis.

We also offer 1.0 µm FluoSpheres® microsphere preparations that contain a much higher dye content than our other FluoSpheres® products. This allows stronger signals to be generated using fewer microspheres per tracing experiment.

In many biological systems, the concentrated fluorescence and spherical shape of the FluoSpheres® beads permit them to be detected against a relatively high but diffuse background fluorescence. Typically for these types of studies, the microspheres and the fluorescent dyes they contain are first extracted from the tissue sample, and then the fluorescence is quantified on a spectrofluorometer or fluorescence microplate reader. The use of fluorescent microspheres eliminates the radiation hazards and disposal hurdles associated with radiolabeled microspheres.

FluoSpheres® Microsphere Specifications:

Label (Ex/Em): Red fluorescent (580/605)
Nominal bead diameter: 1.0 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Carboxylate Coupling Surface
Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.

Choices for FluoSpheres® Fluorescent Microspheres
In addition to microspheres for tracing, investigate our complete offering of fluorescent microspheres products. Among those products you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we stand by quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Amine-Modified Microspheres, 0.2 µm, yellow-green fluorescent (505/515), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green (505/515)
Nominal bead diameter: 0.2 µm
Coupling surface: Amine
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Biotin-Labeled Microspheres, 1.0 µm, yellow-green fluorescent (505/515), 1% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green fluorescent (505 / 515)
Nominal bead diameter: 1.0 µm
Surface modification: Biotin
Solids: 1%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.1 µm, yellow-green fluorescent (505/515), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green (505/515)
Nominal bead diameter: 0.1 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.02 µm, red fluorescent (580/605), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Red (580/605)
Nominal bead diameter: 0.02 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.04 µm, red-orange fluorescent (565/580), 5% solids, azide free (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy.

For tracking particles and cells, tracing fluid dynamics, discriminating the size dependence of uptake or transport in vivo, etc., we offer more concentrated, 0.04 µm diameter FluoSpheres® microspheres preparations (5% solids) that lack the sodium azide preservative present in the majority of our FluoSpheres® products. The 0.04 µm particles are small enough to be microinjected or to be taken up by phagocytosis.

We also offer 1.0 µm FluoSpheres® microsphere preparations that contain a much higher dye content than our other FluoSpheres® products. This allows stronger signals to be generated using fewer microspheres per tracing experiment.

In many biological systems, the concentrated fluorescence and spherical shape of the FluoSpheres® beads permit them to be detected against a relatively high but diffuse background fluorescence. Typically for these types of studies, the microspheres and the fluorescent dyes they contain are first extracted from the tissue sample, and then the fluorescence is quantified on a spectrofluorometer or fluorescence microplate reader. The use of fluorescent microspheres eliminates the radiation hazards and disposal hurdles associated with radiolabeled microspheres.

FluoSpheres® Microsphere Specifications:

Label (Ex/Em): Red-orange fluorescent (565 / 580)
Nominal bead diameter: 0.04 µm
Coupling surface: Carboxylate
Solids: 5%

Characteristics of the Carboxylate Coupling Surface
Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.

Choices for FluoSpheres® Fluorescent Microspheres
In addition to microspheres for tracing, investigate our complete offering of fluorescent microspheres products. Among those products you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we stand by quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 1.0 µm, orange fluorescent (540/560), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Orange (540/560)
Nominal bead diameter: 1.0 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 2.0 µm, red fluorescent (580/605), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Red (580/605)
Nominal bead diameter: 2.0 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Size Kit #1, Carboxylate-modified Microspheres, red fluorescent (580/605), 2% solids, six sizes (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. This FluoSpheres® Size Kit supplies 1 mL of each the 0.02, 0.1, 0.2, 0.5, 1.0, and 2.0 µm sizes.

FluoSpheres® Microsphere Specifications
Label (Ex/Em): Red fluorescent (580/605)
Nominal bead diameter: 0.02, 0.1, 0.2, 0.5, 1.0, 2.0 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin.
• Aldehyde—sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide.

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.02 µm, dark red fluorescent (660/680), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Dark red (660/680)
Nominal bead diameter: 0.02 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 1.0 µm, crimson fluorescent (625/645), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Crimson (625/645)
Nominal bead diameter: 1.0 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Sulfate Microspheres, 4.0 µm, red fluorescent (580/605), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Red (580/605)
Nominal bead diameter: 4.0 µm
Coupling surface: Sulfate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.02 µm, blue fluorescent (365/415), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Blue (365/415)
Nominal bead diameter: 0.02 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Sulfate Microspheres, 1.0 µm, yellow-green fluorescent (505/515), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green (505/515)
Nominal bead diameter: 1.0 µm
Coupling surface: Sulfate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ NeutrAvidin™-Labeled Microspheres, 1.0 µm, nonfluorescent, 1% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): No fluorescent label
Nominal bead diameter: 1.0 µm
Surface modification: NeutrAvidin®
Solids: 1%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ NeutrAvidin™-Labeled Microspheres, 1.0 µm, red fluorescent (580/605), 1% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Red fluorescent (580/605)
Nominal bead diameter: 1.0 µm
Surface modification: NeutrAvidin®
Solids: 1%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

TransFluoSpheres™ Carboxylate-Modified Microspheres, 1.0 µm (488/560), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® TransFluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with two (or more) fluorescent dyes to produce a microspheres that can be excited by the 488 nm or the 514 nm spectral lines of the argon-ion laser but emit at much longer wavelengths, facilitating multicolor experiments and allowing the use of broadband excitation and emission filters. TransFluoSpheres® microspheres typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy.

TransFluoSpheres® Microsphere Specifications:

Label (Ex/Em): Orange-fluorescent (488/560)
Nominal bead diameter: 1.0 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Carboxylate Coupling Surface
Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.

Key Applications of Microspheres:
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.1 µm, infrared fluorescent (715/755), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Infrared (715/755)
Nominal bead diameter: 0.1 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.5 µm, red fluorescent (580/605), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Red (580/605)
Nominal bead diameter: 0.5 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Polystyrene Microspheres, 1.0 µm, orange fluorescent (540/560), for tracer studies (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy.

For tracking particles and cells, tracing fluid dynamics, discriminating the size dependence of uptake or transport in vivo, etc., we offer more concentrated, 0.04 µm diameter FluoSpheres® microspheres preparations (5% solids) that lack the sodium azide preservative present in the majority of our FluoSpheres® products. The 0.04 µm particles are small enough to be microinjected or to be taken up by phagocytosis.

We also offer 1.0 µm FluoSpheres® microsphere preparations that contain a much higher dye content than our other FluoSpheres® products. This allows stronger signals to be generated using fewer microspheres per tracing experiment.

In many biological systems, the concentrated fluorescence and spherical shape of the FluoSpheres® beads permit them to be detected against a relatively high but diffuse background fluorescence. Typically for these types of studies, the microspheres and the fluorescent dyes they contain are first extracted from the tissue sample, and then the fluorescence is quantified on a spectrofluorometer or fluorescence microplate reader. The use of fluorescent microspheres eliminates the radiation hazards and disposal hurdles associated with radiolabeled microspheres.

FluoSpheres® Microsphere Specifications:

Label (Ex/Em): Orange fluorescent (540/560)
Nominal bead diameter: 1.0 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Carboxylate Coupling Surface
Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.

Choices for FluoSpheres® Fluorescent Microspheres
In addition to microspheres for tracing, investigate our complete offering of fluorescent microspheres products. Among those products you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we stand by quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Amine-Modified Microspheres, 0.2 µm, red fluorescent (580/605), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Red (580/605)
Nominal bead diameter: 0.2 µm
Coupling surface: Amine
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Biotin-Labeled Microspheres, 0.2 µm, yellow-green fluorescent (505/515), 1% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Yellow-green fluorescent (505 / 515)
Nominal bead diameter: 0.2 µm
Surface modification: Biotin
Solids: 1%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 0.1 µm, red fluorescent (580/605), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Red (580/605)
Nominal bead diameter: 0.1 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

Rainbow Calibration Particles (Invitrogen™)

Rainbow Calibration Particles are used for calibrating flow cytometers. The mixture of particles features:
• 8 different fluorescent intensities
• Excitation by wavelengths ranging from 365–650 nm
• Particles 3.0–3.4 µm in size

FluoSpheres™ Carboxylate-Modified Microspheres, 0.04 µm, red fluorescent (580/605), 5% solids, azide free (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy.

For tracking particles and cells, tracing fluid dynamics, discriminating the size dependence of uptake or transport in vivo, etc., we offer more concentrated, 0.04 µm diameter FluoSpheres® microspheres preparations (5% solids) that lack the sodium azide preservative present in the majority of our FluoSpheres® products. The 0.04 µm particles are small enough to be microinjected or to be taken up by phagocytosis.

We also offer 1.0 µm FluoSpheres® microsphere preparations that contain a much higher dye content than our other FluoSpheres® products. This allows stronger signals to be generated using fewer microspheres per tracing experiment.

In many biological systems, the concentrated fluorescence and spherical shape of the FluoSpheres® beads permit them to be detected against a relatively high but diffuse background fluorescence. Typically for these types of studies, the microspheres and the fluorescent dyes they contain are first extracted from the tissue sample, and then the fluorescence is quantified on a spectrofluorometer or fluorescence microplate reader. The use of fluorescent microspheres eliminates the radiation hazards and disposal hurdles associated with radiolabeled microspheres.

FluoSpheres® Microsphere Specifications:

Label (Ex/Em): Red fluorescent (580/605)
Nominal bead diameter: 0.04 µm
Coupling surface: Carboxylate
Solids: 5%

Characteristics of the Carboxylate Coupling Surface
Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.

Choices for FluoSpheres® Fluorescent Microspheres
In addition to microspheres for tracing, investigate our complete offering of fluorescent microspheres products. Among those products you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we stand by quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

FluoSpheres™ Carboxylate-Modified Microspheres, 2.0 µm, Nile Red fluorescent (535/575), 2% solids (Invitrogen™)

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres® Microsphere Specifications

Label (Ex/Em): Nile red (535/575)
Nominal bead diameter: 2.0 µm
Coupling surface: Carboxylate
Solids: 2%

Characteristics of the Various FluoSpheres® Coupling Surfaces
• Carboxylate-modified FluoSpheres® beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
• Sulfate FluoSpheres® beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
• Aldehyde-sulfate FluoSpheres® beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
• Amine-modified FluoSpheres® beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres® Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 µm, 0.04 µm, 0.1 µm, 0.2 µm, 0.5 µm, 1.0 µm, 2.0 µm, 4.0 µm, 10.0 µm, and 15.0 µm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. For example, FluoSpheres® beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.