Shop All HPLC Columns

Syncronis™ aQ C18 Polar Endcapped HPLC Columns Thermo Scientific™

Ensure consistent, predictable liquid chromatography (LC) separations of polar analytes using Thermo Scientific™ Syncronis™ aQ Columns. High surface area silica and dense stationary phase coverage minimize secondary interactions and deliver reproducible high-resolution separations. The polar end-capped C18 phase provides optimal retention of polar analytes and is fully compatible with 100% aqueous mobile phases. Manufactured and rigorously tested in ISO 9000 facilities, these columns are designed to meet a range of analytical needs including fast method development, quantitative analysis, and quality control.

Syncronis™ HILIC HPLC Columns Thermo Scientific™

Ensure consistent, predictable separations of polar and hydrophilic analytes with Thermo Scientific™ Syncronis™ HILIC LC Columns. Highly pure, high surface area silica and dense stationary phase coverage minimize secondary interactions and deliver reproducible high-resolution separations. The zwitterionic stationary phase results in a neutral, highly polar surface and provides alternative selectivity to C18 columns without ion-pairing or derivatization. The highly organic mobile phases used with Syncronis HILIC phases facilitate efficient desolvation in ESI-MS detection and improved sensitivity.

Hypersil™ ODS C18 Columns Thermo Scientific™

Choose Thermo Scientific™ Hypersil™ ODS C18 HPLC Columns for a general purpose C18 phase to use in a broad range of applications.

Hypersil stationary phases are recognized as an industry standard in HPLC. Proven as an effective analytical tool, the classical Hypersil phases are well established and referenced in many HPLC methods worldwide. Quality is assured by manufacture under ISO 9001-2000 quality guidelines.

For more challenging separations that require improved chromatographic performance, we recommend the use of Thermo Scientific™ Hypersil GOLD™ columns as an alternative.

Syncronis™ HILIC HPLC Columns Thermo Scientific™

Maintain consistent, predictable separations for your scale up purification needs with Thermo Scientific™ Syncronis™ HILIC preparative HPLC columns for polar and hydrophilic analytes. Highly pure, high surface area silica and dense stationary phase coverage minimize secondary interactions and deliver reproducible high-resolution separations. The zwitterionic stationary phase results in a neutral, highly polar surface and provides optional selectivity to C18 columns without ion-pairing or derivatization. Our columns come in a wide range of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed.

MAbPac™ Reversed Phase HPLC Columns Thermo Scientific™

Use the new Thermo Scientific™ MAbPac™ Reversed Phase columns for high-performance reversed-phase (RP) HPLC and LC/MS characterization of monoclonal antibodies (mAbs), fragments, variants, antibody drug conjugates (ADCs), PEGylated proteins, and bispecific proteins. These columns have excellent resolution, long column lifetime, and low carry-over. The surface chemistry, particle size, and pore size, have been optimized for high resolution separations.

Hypersil™ PREP BDS Si HPLC Columns Thermo Scientific™

Easily scale up your analytical method using Thermo Scientific™ Hypersil™ PREP BDS Si preparative HPLC columns which are packed with a base deactivated silica for normal phase chromatography of nonpolar and moderately polar organic compounds. Our preparative HPLC columns come in a variety of dimensions including an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed.

MAbPac™ Protein A Antibody Analysis and Purification HPLC Column, 12 μm, 4 mm x 35 mm Thermo Scientific™

Run fast, accurate titer analysis of monoclonal antibodies (mAbs) in harvest cell cultures with the Thermo Scientific™ MAbPac™ Protein A LC Columns. This column provides high throughput and accurate analyses through a combination of low backpressure and high efficiency. Its unique format makes automation of loading, binding, elution and collection using biocompatible systems quick and easy. A nonporous, polymeric resin with a hydrophobic divinylbenzene core and a hydrophilic surface optimized for affinity separation makes this column rugged and reproducible.

Acclaim™ Trinity P2 HPLC Columns Thermo Scientific™

Separate pharmaceutical counterions, including monovalent and divalent cations or anions, with Thermo Scientific™ Acclaim™ Trinity P2 LC Columns. The innovative, mixed-mode column technology provides hydrophilic interaction, HILIC, anion-exchange, and cation-exchange functionalities for flexible method development. Use the Acclaim Trinity P2 LC column to complement the Acclaim Trinity P1 LC column, which simultaneously separates pharmaceutical drug substances and their counterions, to provide a total solution for pharmaceutical counterion analysis by HPLC.

Hypersil™ BDS Cyano HPLC Columns Thermo Scientific™

For reliable, general purpose cyano columns, choose the base-deactivated silica Thermo Scientific™ Hypersil™ BDS Cyano LC Columns. These economical columns feature reduced silanol activity and provide exceptional reliability for QA/QC labs. Built on the renowned Hypersil silica backbone, the base-deactivated silica is one of the most popular packing materials available. For more challenging separations that require improved chromatographic performance, use Thermo Scientific™ Hypersil GOLD™ Cyano LC Columns as an alternative.

Hypersil™ PREP BDS C18 HPLC Columns Thermo Scientific™

Easily scale up your analytical method using Thermo Scientific™ Hypersil™ PREP BDS C18 reversed phase preparative HPLC Columns. Our prep columns come in a variety of dimensions including an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed. These endcapped, silica-based columns offer C18 retention with base deactivation for reduced silanol activity and are an excellent choices for routine applications which require development from analytical to preparative LC scale.

Acclaim™ 120 C18 Columns Thermo Scientific™

Improve the separation efficiency in your reversed-phase applications with Thermo Scientific™ Acclaim™ 120 C18 columns. These columns feature an ultrapure silica substrate with extremely low metal content to minimize tailing effects and deliver symmetrical peak shapes. The unique bonding chemistry yields excellent surface coverage to provide highly predictable separations that are unaffected by secondary interactions. Use these high-efficiency columns for maximum resolution in a wide variety of applications.

ProPac™ WCX-10 Carboxylate Ion Exchange HPLC Column, 10 μm, 4 mm x 50 mm Thermo Scientific™

Use Thermo Scientific™ ProPac™ WCX-10 LC Columns to provide exceptionally high resolution for separations of proteins and their variants. Based on stationary phases composed of 10µm nonporous polymeric beads, ProPac columns resolve isoforms that differ by a single charged residue. A thin, hydrophilic layer grafted to the particle core eliminates unwanted secondary interactions. The cation-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.

Syncronis™ C18 Columns Thermo Scientific™

Achieve repeatable and consistent liquid chromatography (LC) separations using Thermo Scientific™ Syncronis™ C18 columns. Highly pure, high surface area silica and dense stationary phase coverage minimize secondary interactions and ensure robust, high-resolution separations. Double end-capping provides extra surface coverage and inertness toward basic compounds. Manufactured and rigorously tested in ISO 9000 facilities, these columns are designed to meet a range of analytical needs including fast method development, quantitative analysis, and quality control.

Acclaim™ Mixed Mode HILIC-1 HPLC Columns Thermo Scientific™

Get all the advantages of reversed-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) in one column with the Thermo Scientific™ Acclaim™ Mixed-Mode HILIC-1 column. The high-efficiency, silica-based, mixed-mode stationary phase consists of a hydrophobic alkyl chain with a diol group at the terminus. The hydrophobic moiety supplies reversed-phase retention and the terminal diol group facilitates hydrophilic interactions. This unique combination provides adjustable selectivity with unsurpassed resolution and peak symmetry for a variety of both polar and nonpolar molecules.

HyPURITY™ ADVANCE™ HPLC Columns, 5µm Particle Size

Thermo Scientific™ HyPURITY ADVANCE™ HPLC columns combine the outstanding performance of our HyPURITY 190Å silica with a C8 reversed phase chemistry. This specialized packing with polar embedded character is well suited for LC/MS applications. The polar group protects the surface silanols from interaction with polar analytes and provides an alternate selectivity with excellent performance for compounds showing poor peak shape with traditional alkyl bonded phases such as C18.

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