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Genomics and Proteomics


Pierce™ Recombinant Protein A Thermo Scientific™

Use Thermo Scientific Pierce Recombinant Peroxidase Conjugated Protein A to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

Features of Recombinant Peroxidase Conjugated Protein A:

• Contains four Fc-binding domains per protein
• Better than Protein G for polyclonal IgG from rabbit, pig, dog and cat serum
• Poorer than Protein G for mouse IgG1, human IgG3 and rat, goat and cow antibodies

Protein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in E. coli and functions essentially the same as native Protein A. The Protein A molecule contains four high-affinity binding sites capable of interacting with the Fc region from IgG of several species including human and rabbit. Optimal binding occurs at pH 8.2, although binding is also effective at neutral or physiological conditions (pH 7.0 to 7.6).

Properties of Recombinant Protein A:
• Source: E. coli
• Molecular Weight: ~44,600 (Apparent MW by SDS-PAGE: 45,000)
• Form: salt-free powder
• A280 of 0.1% solution: ~0.165
• Isoelectric point (pI): 4.7-4.8

The interaction between Protein A and IgG is not equivalent for all species. Even within a species, Protein A interacts with some subclasses of IgG and not others. For instance, human IgG1, IgG2 and IgG4 bind strongly, while IgG3 does not bind. There are also many instances in which monoclonal antibodies do not bind to Protein A, especially the majority of rat immunoglobulins and mouse IgG1.

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Pierce™ Recombinant Protein A

Pierce™ Fast Western Blot Kits, SuperSignal™ West Dura, Rabbit Thermo Scientific™

The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Dura Substrate provides reagents and protocol for reliable and rapid, 1-hour chemiluminescent Western blotting at femtogram levels of sensitivity.

Features of this kit:

Fast—femtogram levels of detection in about 1 hour
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Economical—no expensive consumables or extra equipment
Excellent stability—kit is stable for 1 year stored at 4°C
Easy—complete kits contain all components needed to block, probe and develop a blot with your rabbit primary antibody

The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classical Western blotting with SuperSignal West Dura Chemiluminescent Substrate. Like other Western blotting methods, the included SuperSignal West Dura Substrate produces a signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with rabbit primary antibody.

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Pierce™ Fast Western Blot Kits, SuperSignal™ West Dura, Mouse

Pierce™ Fast Western Blot Kits, SuperSignal™ West Femto, Mouse Thermo Scientific™

The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Femto Substrate contains optimized reagents and protocol for mid-to-low femtogram-level detection and typical Western blot development less than 1 hour after gel transfer.

Features of this kit:

Fast—femtogram levels of detection in about 1 hour
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Easy—complete kits contain all components needed to block, probe and develop a blot with your mouse primary antibody

The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Femto Chemiluminescent Substrate. The kits include SuperSignal West Femto Substrate, the most sensitive enhanced chemiluminescent substrate available for detecting horseradish peroxidase (HRP). This substrate's sensitivity, intensity and duration of the signal allow for easy documentation using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with a mouse primary antibody.

Related Products
Pierce™ Fast Western Blot Kits, SuperSignal™ West Femto, Rabbit

1-Step™ Ultra TMB-Blotting Solution Thermo Scientific™

Thermo Scientific 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting.

This precipitating, colorimetric Western blot substrate for HRP provides high sensitivity, increased signal-to-noise ratio and low background compared to many other chromogenic substrates. The blotting solution contains soluble TMB (3,3',5,5'-tetramethylbenzidine), which reacts very quickly with horseradish peroxidase enzyme to produce an insoluble dark blue precipitate that does not fade or flake. The substrate is compatible with both nitrocellulose and PVDF membranes. The blotting solution is supplied ready to use with no mixing required.

Features of 1-Step Ultra TMB-Blotting Solution:

Fast—protein bands visible in less than one minute
Fade-resistant—protein bands stable after membrane drying
Sensitive—detection limit similar to Pierce ECL Western Blotting Substrate
Chromogenic—no special equipment needed for visualization; produces dark blue bands
Ready to use—no organic solvents are required to dissolve; no dilution necessary for use

Applications:
• Chromogenic Western blot detection
• Nitrocellulose membrane blots
• PVDF membrane blots

Pierce™ Fast Western Blot Kits, SuperSignal™ West Femto, Rabbit Thermo Scientific™

The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Femto Substrate contains optimized reagents and protocol for mid-to-low femtogram-level detection and typical Western blot development less than 1 hour after gel transfer.

Features of this kit:

Fast—femtogram levels of detection in about 1 hour
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Easy—complete kits contain all components needed to block, probe and develop a blot with your rabbit primary antibody

The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Femto Chemiluminescent Substrate. The kits include SuperSignal West Femto Substrate, the most sensitive enhanced chemiluminescent substrate available for detecting horseradish peroxidase (HRP). This substrate's sensitivity, intensity and duration of the signal allow for easy documentation using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with a rabbit primary antibody.

Related Products
Pierce™ Fast Western Blot Kits, SuperSignal™ West Femto, Mouse

Qdot™ 605 Biotin Conjugate Kit Invitrogen™

The biotin-labeled Qdot® 605 nanocrystals are available for detecting streptavidin probes or for creating noncovalent conjugates with streptavidin-labeled molecules or with other biotinylated molecules using a streptavidin bridge. The product is provided as 250 µL of a 2 µM solution and includes 30 mL of Qdot® incubation buffer.

Protein A, Alexa Fluor™ 488 conjugate Invitrogen™

Our Alexa Fluor 488 protein A conjugate binds to the Fc portion of various immunoglobulins from several different species. This exceptionally bright, green-fluorescent probe (absorption/emission maxima ~495/519 nm) can be used to visualize antibodies in a manner analogous to a labeled species-specific secondary antibody.

Pierce™ Fast Blocking Buffer Thermo Scientific™

Pierce Fast Blocking Buffer effectively and reliably blocks western blots in just five minutes to provide low-background results for traditional western blotting protocols.

Compare and view all available blocking buffers

Pierce Fast Blocking Buffer streamlines western blot protocols by providing consistent, high-quality blocking for nitrocellulose or PVDF membranes in only five minutes, producing results comparable to traditional blockers. Blocking and probing steps with Fast Blocking Buffer prevent nonspecific binding of detection antibodies during probing steps but allows specific detection to occur. Pierce Fast Wash Buffer provides efficient membrane washing after incubation with a primary and secondary antibody to effectively shorten total washing time to 20 minutes (from the typical 90 minutes). This buffer is compatible with antibodies and biotin-avidin systems, as well as both nitrocellulose and PVDF membranes.

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Pierce Fast Wash Buffer, 10X

Owl™ VEP-2 Mini Tank Electroblotting System Thermo Scientific™

Produce uniform, reproducible protein transfers for up to four polyacrylamide minigels with the Thermo Scientific™ Owl™ VEP-2 Mini Tank Electroblotting System, ideal for fast, efficient transfer of proteins over a wide molecular weight range.

Pierce™ Fast Western Blot Kit, ECL Substrate Thermo Scientific™

The Thermo Scientific Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

Features of this kit:

Fast—all the sensitivity of Pierce ECL substrate and saving 4 to 5 hours per blot
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Economical—cost as little as $8 per blot; no expensive consumables or extra equipment
Excellent stability—kit is stable for 1 year stored at 4°C
Easy—complete kit gives you all the components you need to block and probe and develop a blot with your mouse or rabbit primary antibody

The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL. The included Pierce ECL Substrate produces a chemiluminescent signal, which is detected using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the primary antibody and immunodetection reagents and reprobed.

The Pierce Fast Western Blot Kit is reagent-based system that provides optimized reagents for blocking, antibody dilution and detection of Western blots with Pierce ECL substrate. The protocol is a quick, efficient and economic way to obtain Western blot results without the hassle of buying an instrument and the consumables required by other popular rapid Western blotting systems. No vacuum pump is needed so there are never any clogged lines or membranes that can occur when using instrument-based systems. Because there is no apparatus, there is no need to buy disposable blotting trays that limit the number of blots processed at one time.

protein A, Alexa Fluor™ 546 conjugate Invitrogen™

Our Alexa Fluor 546 protein A conjugate binds to the Fc portion of various immunoglobulins from several different species. This exceptionally bright, orange-fluorescent probe (absorption/emission maxima ~556/573 nm) can be used to visualize antibodies in a manner analogous to a labeled species-specific secondary antibody.

Pierce™ High Sensitivity NeutrAvidin™-HRP Thermo Scientific™

Thermo Scientific Pierce High Sensitivity NeutrAvidin HRP Conjugate is a specially prepared peroxidase-conjugated form of avidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

Features of High Sensitivity NeutrAvidin HRP Conjugate:

NeutrAvidin Protein—a specially deglycosylated form of avidin that provides highly specific, low-background binding of biotinylated antibodies in many applications.
High sensitivity—detect low levels of target without background; obtain high signal-to-noise ratios
Cost effective—use less conjugate in Western blotting and ELISA applications and still obtain excellent results
Flexible—compatible with typical chemiluminescent, fluorescent and colorimetric peroxidase substrates
Convenient—ready-to-use stabilized liquid format means there is no waiting for thawing and no need to aliquot

This specially-manufactured variety of HRP-conjugated avidin protein meets the demands of today's scientists for more sensitive detection in ELISA and Western blotting applications. The conjugate is suitable for use with chemiluminescent, chemifluorescent or colorimetric substrates. Each High Sensitivity HRP conjugate is packaged in an easy-to-use stabilized solution that enables convenient storage at 4°C for at least one year.

Our High Sensitivity HRP Conjugates outperform the competition on target detection, signal intensity and signal-to-noise ratios. For example, experiments indicate that the High Sensitivity Streptavidin-HRP can be diluted eight-to 10-fold more than traditional HRP conjugates and still produce similar results.

iBind™ Flex Fluorescent Detection (FD) Solution Kit Invitrogen™

iBind Flex Fluorescent Detection (FD) Solution is compatible with downstream fluorescent detection (e.g., infrared dual wavelength) and serves as a combined blocking, washing, and antibody diluent solution to process up to one midi blot, two mini blots, or six vertically cut strip blots when used with the iBind Flex Western Device and iBind Flex Cards. Each kit contains reagents sufficient for 10 midi blots or 20 mini blots.

Learn more about the iBind western systems ›

iBind Flex Western System
The iBind Flex Western System offers automated western blot processing and can accommodate various blot formats including midi, mini, and vertically cut strips. Based on Sequential Lateral Flow (SLF) technology, all blocking, antibody incubation and washing steps during immunodetection are hands-free. Compared to manual methods, the iBind Flex Western System requires less primary antibody, enables reproducible results and is compatible with downstream detection protocols.

SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto Thermo Scientific™

This substrate bundle contains SuperSignal West Femto Maximum Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate to enable trial and comparison of the newest and most sensitive substrate for low abundant targets.

SuperSignal West Femto Maximum Sensitivity Substrate
Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is a high-sensitivity enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. It is optimized to detect low abundant and/or difficult-to-detect targets.

• Sensitivity: low-femtogram (mid-zeptomole)
• Stability: eight hours for working solution, one year for kit at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to eight hours
• Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL)
• Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)

View full product information ›

SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate
Thermo Scientific SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for high attogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. It is designed for detection of very low-abundant targets or the maximum levels of sensitivity required when using precious samples or costly antibodies.

• Sensitivity: low-femtogram to high-attogram
• Stability: eight hours for working solution, one year for kit at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to four hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 µg/mL)
• Recommended secondary antibody concentration: 1:100,000–1:250,000 dilution (4–10 ng/mL)

View full product information ›

Owl™ VEP-3 Large Tank Electroblotting System Thermo Scientific™

Rapidly transfer high molecular weight proteins with the Thermo Scientific™ Owl™ VEP-3 Large Tank Electroblotting System.
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