Protein Method Blocking Buffers

Protein method blocking buffers are used to block plates, membranes, and other sample types, and decrease incubation time and background signal for use during enzyme-linked immunosorbent assays (ELISA) and western blot experiments. These products are available in different concentrations and formulations.
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Membrane Blocking Solution Invitrogen™

Membrane Blocking Solution can be used to block nitrocellulose and PVDF membranes, as well as to dilute antibodies or enzyme conjugates. This solution is a Tris buffer containing bovine serum albumin, goat IgG, Tween 20, and a mixture of other components that can reduce non-specific binding.

eBioscience™ IHC /ICC Blocking Buffer - Low Protein Invitrogen™

The IHC/ICC Blocking Buffer- Low Protein is designed for use in immunohistochemistry and immunocytochemistry protocols which require blocking of non-specific binding sites. This blocking buffer is recommended for tissues and antibodies with average levels of background staining.

TRIS-buffered saline (TBS, 20X), with 2% Tween 20 Thermo Scientific™

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

Tween 20 washing buffer, 1.0% in PBS (20X) Thermo Scientific™

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

TRIS-buffered saline (TBS, 10X), with 1% Tween 20 Thermo Scientific™

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

BSA Blocking Buffer, 3% in PBS Thermo Scientific™

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

BSA Blocking Buffer, 5% in TBS Thermo Scientific™

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

BSA Blocking Buffer, 3% in PBS, with 0.05% Tween-20 Thermo Scientific™

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

Tween 20 blocking buffer, 1% in PBS (10X) Thermo Scientific™

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

BSA Blocking Buffer, 5% in TBS, with 0.02% sodium azide Thermo Scientific™

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

Casein, Hammarsten grade Thermo Scientific™

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

BSA Blocking Buffer, 5% in TBS, with 0.05% Tween-20 Thermo Scientific™

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

BSA Blocking Buffer, 5% in PBS Thermo Scientific™

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

Blocker™ Casein in PBS Thermo Scientific™

Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods. Blocker Casein is 1% (w/v) casein, which corresponds to the optimal concentration for most applications.

Blocker™ BSA (10X) in PBS Thermo Scientific™

Blocker BSA (10X) is a purified 10% solution of bovine serum albumin for blocking in western blotting, ELISA, IHC, and nucleic acid detection methods. Compare and view all available blocking buffers Blocker BSA is a 10% (w/v) solution of high-quality BSA that is useful for saturating excess...
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