Shop All Protein Method Blocking Buffers

eBioscience™ IHC/ICC Blocking Buffer - High Protein Invitrogen™

The IHC/ICC Blocking Buffer- High Protein is designed for use in immunohistochemistry and immunocytochemistry protocols which require blocking of non-specific binding sites. This blocking buffer is recommended for tissues and antibodies with high background staining, nuclear antigens, and FFPE tissue. This blocking buffer can be used both in the blocking step and as a diluent for primary and secondary antibodies during antibody incubation steps. The IHC/ICC Blocking Buffer-High Protein is compatible with organic dye-conjugated antibodies as well as unconjugated antibodies that may be used in two and three step staining protocols. This buffer is supplied as a 1X stock solution.

Reported Application
Immunohistochemical Staining, Immunocytochemistry, Immunohistochemical Staining of Frozen Tissue Sections, Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections

Pierce™ Protein-Free (TBS) Blocking Buffer Thermo Scientific™

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a ready-to-use format.

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Features of Protein-Free Blocking Buffer:
Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
Applications—western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)

Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.

Related products
Pierce Protein-Free (PBS) Blocking Buffer
Pierce Protein-Free T20 (PBS) Blocking Buffer
Pierce Protein-Free T20 (TBS) Blocking Buffer

Pierce™ Protein-Free (PBS) Blocking Buffer Thermo Scientific™

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a ready-to-use format.

Compare and view all available blocking buffers

Features of Protein-Free Blocking Buffer:
Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
Applications—western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)

Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.

Related products
Pierce Protein-Free T20 (PBS) Blocking Buffer
Pierce Protein-Free (TBS) Blocking Buffer
Pierce Protein-Free T20 (TBS) Blocking Buffer

eBioscience™ IHC /ICC Blocking Buffer - Low Protein Invitrogen™

The IHC/ICC Blocking Buffer- Low Protein is designed for use in immunohistochemistry and immunocytochemistry protocols which require blocking of non-specific binding sites. This blocking buffer is recommended for tissues and antibodies with average levels of background staining. This blocking buffer can be used both in the blocking step and as a diluent for unconjugated antibodies and organic dye-conjugated antibodies during the antibody incubation step. This buffer is supplied as a 1X stock solution containing sodium azide.

Reported Application
Immunohistochemical Staining, Immunocytochemistry, Immunohistochemical Staining of Frozen Tissue Sections, Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections

Blocker™ Casein in PBS Thermo Scientific™

Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods. Blocker Casein is 1% (w/v) casein, which corresponds to the optimal concentration for most applications.

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Features:
Purified casein—single-protein blocking buffer provides fewer chances of cross-reaction with assay components than serum or milk solutions
Easy to use—1% casein solution is ready to use; can be diluted further as needed
Safe—stable, thimerosal-free formulation

Related products
Blocker Casein in PBS

Pierce™ Fast Blocking Buffer Thermo Scientific™

Pierce Fast Blocking Buffer effectively and reliably blocks western blots in just five minutes to provide low-background results for traditional western blotting protocols.

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Pierce Fast Blocking Buffer streamlines western blot protocols by providing consistent, high-quality blocking for nitrocellulose or PVDF membranes in only five minutes, producing results comparable to traditional blockers. Blocking and probing steps with Fast Blocking Buffer prevent nonspecific binding of detection antibodies during probing steps but allows specific detection to occur. Pierce Fast Wash Buffer provides efficient membrane washing after incubation with a primary and secondary antibody to effectively shorten total washing time to 20 minutes (from the typical 90 minutes). This buffer is compatible with antibodies and biotin-avidin systems, as well as both nitrocellulose and PVDF membranes.

Related products
Pierce Fast Wash Buffer, 10X

Pierce™ Clear Milk Blocking Buffer (10X) Thermo Scientific™

Pierce Clear Milk Blocking Buffer (10X) is a long-shelf life, high-performance replacement for homemade non-fat milk blocking buffers in western blotting applications.

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Our Clear Milk Blocking Buffer (10X) is a pre-formulated milk solution, clarified and stabilized for blocking excess nonspecific binding sites, reducing background in western blotting applications and diluting antibodies when used with nitrocellulose and PVDF membranes. It provides lower background, enhanced sensitivity, extended shelf life, and reproducible results compared to homemade buffers based on dissolved non-fat dry milk. Not recommended for avidin-based techniques because it contains some endogenous biotin.

Blocker™ BSA (10X) in TBS Thermo Scientific™

Blocker BSA (10X) is purified 10% solution of bovine serum albumin for blocking in western blotting, ELISA, IHC and nucleic acid detection methods.

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Blocker BSA is a 10% (w/v) solution of high-quality BSA that is useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. It is most frequently diluted 10-fold (to 1% BSA) in 1X PBS or 1X TBS for initial testing. Blocker BSA is usually more effective than nonfat milk blocking buffers for biotin-avidin systems because it contains a single purified protein that is devoid of endogenous biotin.

Related products
Blocker BSA (10X) in PBS

Blocker™ FL Fluorescent Blocking Buffer (10X) Thermo Scientific™

Blocker FL Fluorescent Blocking Buffer is designed to block excess nonspecific binding sites to help reduce background fluorescence in western blotting applications. Blocker FL buffer helps enhance fluorescent protein detection by increasing the signal-to-noise ratio in blotting applications where background fluorescence may otherwise interfere with signal detection.

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Working reagent is prepared by adding 1 part Blocker FL buffer to 9 parts ultrapure water. Optimal concentration should be determined empirically for each system. Compatible with both nitrocellulose and PVDF membranes.

Pierce™ Protein-Free T20 (TBS) Blocking Buffer Thermo Scientific™

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a ready-to-use format.

Compare and view all available blocking buffers

Features of Protein-Free Blocking Buffer:
Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
Applications—western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)

Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.

Related products
Pierce Protein-Free (PBS) Blocking Buffer
Pierce Protein-Free T20 (PBS) Blocking Buffer
Pierce Protein-Free (TBS) Blocking Buffer

Pierce™ Protein-Free T20 (PBS) Blocking Buffer Thermo Scientific™

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a ready-to-use format.

Compare and view all available blocking buffers

Features of Protein-Free Blocking Buffer:
Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
Applications—western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)

Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.

Related products
Pierce Protein-Free (PBS) Blocking Buffer
Pierce Protein-Free (TBS) Blocking Buffer
Pierce Protein-Free T20 (TBS) Blocking Buffer

SEA BLOCK Blocking Buffer Thermo Scientific™

Thermo Scientific SEA BLOCK Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in IHC and other detection methods involving mammalian samples.

Features of SEA BLOCK Blocking Buffer:

Non-mammalian—fish proteins are less likely to have specific binding interactions with antibodies and other mammalian proteins present in typical methods
Convenient—filtered and stabilized in PBS for compatibility with most assay systems
Easy to use—can be used as supplied or diluted up to 10-fold as needed
Flexible—may be used for many different applications, including as a diluent for antibodies

Because SEA BLOCK Blocking Buffer is fish serum, it is effective in stabilizing solutions and samples for antibody-binding interactions while at the same time minimizing the possibility of cross-reaction with mammalian sample components. When normal sera cause background in immunohistochemistry (IHC), try this product. The product is also especially effective in various cellular imaging protocols based on visible and near-infrared fluorescence detection.

Fish serum has several advantages over typical blocking buffers. Because salmon is phylogenetically distant from mammals, which are the source of antibodies and samples used in most experiments, its serum proteins are less likely to have specific binding interactions with proteins used in typical cell biology experiments. Because SEA BLOCK Blocking Buffer is fish serum, it is effective in stabilizing solutions and samples for antibody-binding interactions. When normal sera cause background in IHC experiments, SEA BLOCK Blocking Buffer may correct the problem.

SEA BLOCK Blocking Buffer is particularly effective in applications involving fluorescence imaging. The blocker has been used to decrease background and increase signal-to-noise ratios with near-IR fluorescent probes and is validated for use with the LI-COR Odyssey™ Infrared Imaging System.

Membrane Blocking Solution Invitrogen™

Membrane Blocking Solution can be used to block nitrocellulose and PVDF membranes, as well as to dilute antibodies or enzyme conjugates. This solution is a Tris buffer containing bovine serum albumin, goat IgG, Tween 20, and a mixture of other components that can reduce non-specific binding. The Proclin used in this reagent does not inhibit peroxidase or alkaline phosphatase activity. Note that this blocking solution contains normal goat IgG and should not be used in assays where anti-goat immunoglobulins are employed.

Pierce™ Fast Wash Buffer, 10X Thermo Scientific™

Pierce Fast Wash Buffer, 10X, for use with Pierce Fast Blocking Buffer.

Related Products
Pierce™ Fast Blocking Buffer

Blocker™ BLOTTO in TBS Thermo Scientific™

Blocker BLOTTO Blocking Buffer is a ready-to-use 5% solution of nonfat powdered milk in Tris-buffered saline for western blot, ELISA, IHC, and other detection methods.

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Features of Blocker BLOTTO Blocking Buffer:
Format—ready-to-use 1X TBS solution; can be diluted as needed
Formulation—formulated with anti-foaming agent and thimerosal-free preservative

Not recommended for avidin-based techniques because it contains some endogenous biotin.

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