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General Western Blot Reagents
General western blot reagents are used in conjuction with analytical western blot experiments and immunoassays. These products include enhancing buffers, transfer solutions, stripping buffers, and chromogenic and chemiluminescent kits and substrates.
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Restore™ PLUS Western Blot Stripping Buffer Thermo Scientific™
Thermo Scientific Restore PLUS Western Blot Stripping Buffer is an advanced formula for removing bound primary and secondary antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Tris-Glycine Transfer Buffer (25X) Invitrogen™
Tris-Glycine Transfer Buffer (25X) is optimized for western blot transfer of proteins to nitrocellulose, PVDF, or nylon membranes for subsequent analysis using Tris-Glycine gels. To use: This buffer should be diluted to a 1X solution with a water/methanol mixture to yield a final methanol...
NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels) Invitrogen™
The NuPAGE MES SDS Buffer Kit is designed for separation of small- to medium-size proteins on NuPAGE Bis-Tris gels and includes the following buffers: • NuPAGE MES SDS Running Buffer (20X, 500 mL, Cat. No. NP0002) • NuPAGE Sample Reducing Agent (10X, 250 µL, Cat. No.
Pierce™ Western Blot Signal Enhancer Thermo Scientific™
Thermo Scientific Pierce Western Blot Signal Enhancer is a two-reagent system for conditioning protein blots after transfer to greatly enhance the effectiveness of primary antibodies and intensify the final detection signal in Western blot experiments.
iBind™ Western Starter Kit Invitrogen™
The iBind Western Starter Kit includes an iBind Western Device as well as all of the consumables and reagents necessary to get started using this automated western-processing device. The iBind Western Device performs every step in the western protocol from blocking to washes to antibody incubations...
NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels) Invitrogen™
The NuPAGE MOPS SDS Buffer Kit is designed for separation of medium- to large-size proteins on NuPAGE Bis-Tris gels and includes the following buffers: • NuPAGE MOPS SDS Running Buffer (20X, 500 mL, Cat. No. NP0001) • NuPAGE Sample Reducing Agent (10X, 250 µL, Cat. No.
iBind™ Flex Western Starter Kit Invitrogen™
The iBind Western Starter Kit includes one iBind Flex Western Device, one box of iBind Flex Cards, and one iBind Flex Solution Kit. The iBind Flex Western Device is an automated western-processing device that performs every step from blocking to washes to antibody incubations via sequential lateral...
Restore™ Fluorescent Western Blot Stripping Buffer Thermo Scientific™
The Thermo Scientific Restore Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from western blots.
WesternBreeze™ Blocker/Diluent (Part A and B) Invitrogen™
The WesternBreeze® Blocker/Diluent (part A and B) is an optimized, easy-to-use blocker and primary antibody diluent system that yields low background/high signal western blot detection on nitrocellulose (NC) and polyvinylidene difluoride (PVDF) membranes. Sufficient reagents for 20 mini-blots.
WesternBreeze™ Chemiluminescent Kit, anti-rabbit Invitrogen™
WesternBreeze® Chemiluminescent Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use CDP-Star® chemiluminescent substrate for alkaline phosphatase.
WesternBreeze™ Chromogenic Kit, anti-mouse Invitrogen™
WesternBreeze® Chromogenic Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use BCIP/NBT substrate for alkaline phosphatase.
No-Stain™ Protein Labeling Reagent Invitrogen™
The Invitrogen No-Stain Protein Labeling Reagent provides a flexible, accurate, rapid, and reliable method to visualize and normalize proteins in a gel or on a membrane (post-transfer). It forms covalent bonds to proteins in gels or on membranes within 10 minutes, does not require any de-staining...
SuperSignal™ Western Blot Enhancer Thermo Scientific™
Thermo Scientific SuperSignal Western Blot Enhancer contains a membrane treatment reagent and a primary antibody diluent that increase both signal intensity and sensitivity 3- to 10-fold compared to a detection performed without it.
TMB Substrate Solution Thermo Scientific™
Addition of sulfuric acid stop solution changes the color to yellow, enabling accurate measurement of the intensity at 450nm using a spectrophotometer or plate reader. This particular formulation is used in most of the Thermo Scientific Pierce ELISA Kits where it provides consistent performance for...
Novex™ Tricine SDS Buffer Kit, includes LC1676 & LC1675 Invitrogen™
The Novex Tricine SDS Buffer Kit is designed for separation of small- to medium-size proteins on tricine gels. This kit includes the following buffers: • Tricine SDS Sample Buffer (Cat. No. LC1676) • Novex Tricine SDS Running Buffer (Cat. No. LC1675) See all buffers and reagents for SDS-PAGE ›
