General Western Blot Reagents

General Western Blot Reagents

General western blot reagents are used in conjuction with analytical western blot experiments and immunoassays. These products include enhancing buffers, transfer solutions, stripping buffers, and chromogenic and chemiluminescent kits and substrates.
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1-15 of 35 Products

Restore™ PLUS Western Blot Stripping Buffer Thermo Scientific™

Thermo Scientific Restore PLUS Western Blot Stripping Buffer is an advanced formula for removing bound primary and secondary antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Tris-Glycine Transfer Buffer (25X) Invitrogen™

Tris-Glycine Transfer Buffer (25X) is optimized for western blot transfer of proteins to nitrocellulose, PVDF, or nylon membranes for subsequent analysis using Tris-Glycine gels. To use: This buffer should be diluted to a 1X solution with a water/methanol mixture to yield a final methanol...

Restore™ Western Blot Stripping Buffer Thermo Scientific™

Thermo Scientific Restore Western Blot Stripping Buffer safely and effectively removes primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Pierce™ Western Blot Signal Enhancer Thermo Scientific™

Thermo Scientific Pierce Western Blot Signal Enhancer is a two-reagent system for conditioning protein blots after transfer to greatly enhance the effectiveness of primary antibodies and intensify the final detection signal in Western blot experiments.

SuperSignal™ Western Blot Enhancer Thermo Scientific™

Thermo Scientific SuperSignal Western Blot Enhancer contains a membrane treatment reagent and a primary antibody diluent that increase both signal intensity and sensitivity 3- to 10-fold compared to a detection performed without it.

NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels) Invitrogen™

The NuPAGE MES SDS Buffer Kit is designed for separation of small- to medium-size proteins on NuPAGE Bis-Tris gels and includes the following buffers: • NuPAGE MES SDS Running Buffer (20X, 500 mL, Cat. No. NP0002) • NuPAGE Sample Reducing Agent (10X, 250 µL, Cat. No.

iBind™ Western Starter Kit

The iBind Western Starter Kit includes an iBind Western Device as well as all of the consumables and reagents necessary to get started using this automated western-processing device. The iBind Western Device performs every step in the western protocol from blocking to washes to antibody incubations...

WesternBreeze™ Blocker/Diluent (Part A and B) Invitrogen™

The WesternBreeze® Blocker/Diluent (part A and B) is an optimized, easy-to-use blocker and primary antibody diluent system that yields low background/high signal western blot detection on nitrocellulose (NC) and polyvinylidene difluoride (PVDF) membranes. Sufficient reagents for 20 mini-blots.

Incubation Tray, 10 x 14 cm Invitrogen™

The Incubation Tray enables convenient and even staining of E-PAGE™ blots. The tray is 10 cm (l) x 14 cm (w) x 3 cm (d) with a lid. It is suitable for use with immunodetection or blot stains.

NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels) Invitrogen™

The NuPAGE MOPS SDS Buffer Kit is designed for separation of medium- to large-size proteins on NuPAGE Bis-Tris gels and includes the following buffers: • NuPAGE MOPS SDS Running Buffer (20X, 500 mL, Cat. No. NP0001) • NuPAGE Sample Reducing Agent (10X, 250 µL, Cat. No.

Pierce™ 1-Step Transfer Buffer Thermo Scientific™

Thermo Scientific Pierce 1-Step Transfer Buffer is designed for rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Power Blotter.

WesternBreeze™ Chromogenic Kit, anti-rabbit Invitrogen™

WesternBreeze® Chromogenic Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use BCIP/NBT substrate for alkaline phosphatase.

WesternBreeze™ Chromogenic Kit, anti-mouse Invitrogen™

WesternBreeze® Chromogenic Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use BCIP/NBT substrate for alkaline phosphatase.

XCell II™ Blot Module Invitrogen™

The XCell II Blot Module is a mini-gel wet-transfer module for the XCell SureLock or XCell II systems. The XCell II Blot Module allows you to easily transfer proteins or nucleic acids for western, southern, and northern transfers from mini gels to membranes with less than 200 mL of transfer buffer.

WesternBreeze™ Chromogenic Kit, anti-goat Invitrogen™

WesternBreeze® Chromogenic Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use BCIP/NBT substrate for alkaline phosphatase.
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