Shop All Western Blot and Nucleic Acid Membranes and Filter Paper

SureLock™ Tandem Midi Pre-cut Membranes and Filters, 0.2 μm, nitrocellulose Invitrogen™

Invitrogen SureLock Tandem Midi Pre-cut Membranes and Filters, 0.2 μm, nitrocellulose, are high-quality membranes ideal for blotting proteins and nucleic acids. The 0.2 µm pore size is ideal for the transfer of low molecular weight proteins (<20 kDa) and nucleic acids (<300 bp). The dimensions (8.6 x 14 cm) of these nitrocellulose membrane and filter paper sandwiches are ideal for wet-transfer of Invitrogen midi gels using the SureLock Tandem Midi Blot Module. The 0.2 µm pore size membrane and filter paper sandwiches are conveniently supplied as pack of 20.

Features
• Membrane: 100% pure nitrocellulose
• Filter paper thickness: 0.8 mm
• Re-probe characteristics: yes
• Binding interactions: hydrophobic and electrostatic
• Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• Dimensions: 8.6 x 14 cm

Owl™ Transfer Membranes Thermo Scientific™

Perform Western blotting with hydrophobic Thermo Scientific™ Owl™ Transfer Membranes.

PVDF Transfer Membrane, 0.2 µm, 26.5 cm x 3.75 m Thermo Scientific™

Pierce PVDF Transfer Membrane, 0.2 µm, is made of high-quality polyvinylidene difluoride and provides high binding capacity for proteins and nucleic acids for western, southern, and northern blotting methods. The 0.2-µm pore size makes this membranes ideal for protein analysis of small amounts of proteins (as little as 10 pmoles), amino acid analysis, and transfer of small molecular weight proteins and peptides.

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Features:
Membrane: polyvinylidene difluoride (PVDF)
Binding capacity: goat IgG: 448 µg/cm2; BSA: 340 µg/cm2; insulin: 262 µg/cm2
Re-probe characteristics: yes
Pre-activation: required with 100% alcohol (methanol)
Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, chemiluminescence, and radiolabeling
Durability: compatible with most organic solvents, acids, and mild bases

Owl™ Blotting Paper Thermo Scientific™

Absorb adequate amounts of buffer for semi-dry electroblotting without the loss of mechanical stability with Thermo Scientific™ Owl™ Blotting Paper, high-quality blotting paper ideal for wicking and blotting applications.

SureLock™ Tandem Midi Pre-cut Membranes and Filters, 0.45 μm, nitrocellulose Invitrogen™

Invitrogen SureLock Tandem Midi Pre-cut Membranes and Filters, 0.45 μm, nitrocellulose, are high-quality membranes ideal for blotting proteins and nucleic acids. The 0.45-µm pore size is ideal for the transfer of most proteins (>20 kDa) and nucleic acids (>300 bp). The dimensions (8.6 x 14 cm) of these nitrocellulose membrane and filter paper sandwiches are ideal for wet-transfer of Invitrogen midi gels using the SureLock Tandem Midi Blot Module. The 0.2 µm pore size membrane and filter paper sandwiches are conveniently supplied as pack of 20.

Features:
• Membrane: 100% pure nitrocellulose
• Filter paper thickness: 0.8 mm
• Re-probe characteristics: yes
• Binding interactions: hydrophobic and electrostatic
• Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• Dimensions: 8.6 x 14 cm

SureLock™ Tandem Midi Pre-cut Membranes and Filters, 0.45 μm, PVDF Invitrogen™

Invitrogen SureLock Tandem Midi Membranes and Filters, 0.45 μm, PVDF, have excellent binding properties for western blotting, dot-blot assays, and protein sequencing. The 0.45-µm pore size makes these membranes ideal for protein transfer for proteins >10 kDa. The dimensions (8.6 x 14 cm) of these PVDF membrane and filter paper sandwiches are ideal for wet-transfer of Invitrogen midi gels using the SureLock Tandem Midi Blot Module. The 0.45-µm pore size membrane and filter paper sandwiches are conveniently supplied as pack of 20.

Features
• Membrane: polyvinylidene difluoride (PVDF)
• Filter paper thickness: 0.8 mm
• Re-probe characteristics: yes
• Pre-activation: required with 100% alcohol (methanol, ethanol, or isopropanol)
• Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, chemiluminescence, and radiolabeling
• Durability: compatible with most organic solvents, acids, and mild bases
• Dimensions: 8.6 x 14 cm

Power Blotter Pre-cut Membranes and Filters, PVDF, regular size Invitrogen™

These Invitrogen Power Blotter Pre-cut Membranes and Filters are designed for the transfer of proteins from two mini-size or one midi-size gel using the Power Blotter System. The pre-cut membranes and filters include 20 sheets of polyvinylidene difluoride (PVDF) and 80 sheets of absorbent blotting paper (0.85 mm thickness). Power Blotter Pre-cut Membranes and Filters combined with Power Blotter 1-Step Transfer Buffer allow for high-efficiency semi-dry transfer of proteins in less than 10 minutes.

Features of PVDF membranes:
High-quality—PVDF transfer membranes manufactured especially for protein transfer and western blot applications
High-binding affinity—provides excellent protein binding (240 µg/cm2), blocks easily, and is compatible with most organic solvents, acids, and mild bases
Convenient—pre-cut PVDF sheets are ready to use for transfer and blotting experiments from two standard precast and homemade mini-gels or one midi-size gel (8 cm x 13 cm); no need to cut your own membranes, minimizing damage and contamination
Validated—membrane and filter when combined provide the thickness and buffer capacity needed for optimal performance in the Power Blotter System

The PVDF membrane (0.45 µm pore size, low fluorescence) has higher binding capacity than nitrocellulose. The membrane is compatible with commonly used detection methods such as staining, immunodetection, and fluorescence. The proteins bind to the membrane through hydrophobic interactions. This membrane has lower background levels and increased sensitivity for fluorescent probing than regular PVDF or nitrocellulose membranes.

Learn more about the Power Blotter System ›

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Power Blotter Pre-cut Membranes and Filters, PVDF, mini
Power Blotter Pre-cut Membranes and Filters, nitrocellulose, mini
Power Blotter Pre-cut Membranes and Filters, nitrocellulose, regular size

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