Shop All Gel Electrophoresis Buffers and Diluents

AEC Solution (IHC)

This Single Solution utilizes aminoethyl carbazole (AEC), an alcohol soluble chromogen, and generates a reddish deposit at the reaction site on tissue or cell preparations. Single Solution AEC is suitable for use with manual or automated staining systems, and is provided as a liquid in a single bottle that can be stored at -8°C.

eBioscience™ Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A) Invitrogen™

The eBioscience Fixation and Permeabilization Kit is designed for use in preparation of living cells for intracellular staining and flow cytometric analysis. A protein transport inhibitor (Brefeldin A) is included for treatment of cells during culture – to cause accumulation of cytokine protein at the Golgi and enhance resulting staining. A fixation buffer is used to cross link, stabilize, and fix the cell membrane, while the permeabilization buffer is used to reversibly permeabilize the cell membrane so the staining antibodies can enter the cell effectively.

Reported Application
Intracellular Staining Followed by Flow Cytometric Analysis

Novex™ pH 3-10 IEF Buffer Kit,Includes LC5300, LC5310, LC5311 Invitrogen™

Novex® pH 3-10 IEF Buffer Kit is optimized for using with Novex® pH 3-10 IEF gels. Each kit includes LC5300, LC5310, LC5311.

Novex™ IEF Cathode Buffer pH 3-7 (10X) Invitrogen™

Novex® IEF Cathode Buffer pH 3-7 (10X) is optimized pre-mixed IEF cathod buffer for using with Novex® pH 3-7 IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent results.

Usage note: The IEF cathode buffer solutions contain a reagent that varies from white to light yellow in color. This color variation may produce a similar color variation in the final solution, from colorless to yellowish in tone. This color will not affect product performance.

ZOOM™ Carrier Ampholytes pH 9-11 Invitrogen™

ZOOM® Carrier Ampholytes are small, soluble molecules with both positive and negative charge groups. They sort based on their isoelectric points in an electric field, thereby establishing a pH gradient in solution or contributing to the gradient when used with IPG strips. Carrier ampholytes help stabilize the pH gradient and current in IPG strips and aid in protein solubility, resulting in reproducible IEF resolution.

Kinase Buffer R (5X) Thermo Scientific™

Kinase buffer that has been specially optimized for use with the Class I & II PI3 kinases.

10X Bolt™ Sample Reducing Agent Invitrogen™

Bolt Sample Reducing Agent (10X) is used to reduce protein samples for protein gel electrophoresis. It contains 500 mM dithiothreitol (DTT) for a 10X concentration in a stabilized liquid form.

See all available buffers and reagents available for SDS-PAGE

Pierce™ Lane Marker Non-Reducing Sample Buffer Thermo Scientific™

Thermo Scientific Pierce Lane Marker Non-Reducing Sample Buffer is a ready-to-use 5X SDS-PAGE sample loading buffer in a non-reducing formulation, with a pink dye buffer-front marker.

Features of Lane Marker Sample Buffer:

Easy to see—bright pink dye marks the buffer front, clearly indicating the progress of the electrophoresis run
Concentrated—5X stocks enable a greater range of protein sample volumes to be used for electrophoresis
Transferable marker dye—pink dye transfers with protein to nitrocellulose membrane, enabling verification of transfer direction and orientation of the blot

Lane Marker Non-Reducing Sample Buffer is convenient and ready to use for SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Formulated as a 5X stock rather than the traditional 2X stock, it enables a larger volume of protein solution to be included in the sample that is loaded in each well. Particularly unique in this buffer is the use of a bright pink tracking dye instead of the traditional bromophenol blue dye. This pink dye marks not only the dye front in the gel but also transfers and permanently binds to nitrocellulose membranes. Consequently, the dye can be used to assess transfer efficiency and align cut membranes.

Lane Marker Sample Buffer is easy to use. Simply mix one part Sample Buffer with four parts protein sample, heat denature, and then load the gel sample wells. Visualize progress of the electrophoresis run by monitoring the location of the pink dye front. Lane Marker Sample Buffer may be used in denaturing gels of all kinds and is compatible with coomassie dye and silver staining techniques, as well as Western blotting procedures.

Note: This product is not compatible with fluorescent detection systems. (The pink tracking dye fluoresces strongly.)

Related Products
Pierce™ Lane Marker Reducing Sample Buffer

Kinase Buffer Q (5X) Thermo Scientific™

Kinase buffer that has been specially optimized for use with PIK3C3 (hVPS34).

ZOOM™ Focusing Buffer pH 3-7 Invitrogen™

The ZOOM® IEF Fractionator offers a fast, reliable method to reduce sample complexity, enrich low abundance proteins, and increase the dynamic range of detection. Solution phase isoelectric focusing with the ZOOM® IEF Fractionator provides reproducible separations in three hours. Fractionated samples are ready for further analysis by two dimensional gel electrophoresis (2DE), one dimensional gel electrophoresis (1DE), or two dimensional liquid chromatography/mass spectrometry (2D LC/MS).

The ZOOM® IEF Fractionator System (Figure 1) consists of the following components:

• ZOOM® IEF Fractionator is built for safety, reliability, and durability. It includes easy-to-assemble sample chambers, in-built buffer chambers, buffer trough, removable electrode assembly, and safety lid.
• ZOOM® Disks are pre-cast polyacrylamide gels that eliminate the need for manual preparation and minimize any chance of cross contamination. These immobilized buffered disks are pre-labeled, disposable, and designed for single use, ensuring consistent and reproducible fractionation. They resolve samples into as many as 7 fractions (using 8 disks of specific pH) to six fractions (using six disks of specific pH) from pH 3-12. Simply place each disk between the sample chambers in the ZOOM® IEF Fractionator to allow separation in a specific pH range.
• ZOOM® Reagents are proteomic grade reagents, including ZOOM® 2D Protein Solubilizers urea, thiourea, CHAPS, and ampholytes, that ensure optimal conditions for sample preparation.
• Basic Protein Kit which includes ZOOM® Discs and ZOOM Strips for IEF of basic proteins, focusing buffers, and solubilizer kits.

Sample Chamber and Fractionator Unit Set-up Procedure
Figures 4-6 illustrate assembly of the sample chambers and fractionator unit. The sample chamber and components fit together easily and offer a leak-proof seal. Simply place disks between the sample chambers in the ZOOM® IEF Fractionator to allow separation in a specific pH range. For example, if you want to fractionate proteins between pH 4.6 and 5.4, flank a sample chamber with a ZOOM (r) Disk of pH 4.6 and one of pH 5.4.

Downstream Processing by 2D Gel Electrophoresis
Following fractionation, the separated samples can be subjected to further analysis using narrow or broad range ZOOM® Strips on the ZOOM® IPGRunner™ System. Follow with second dimension analysis using neutral pH NuPAGE® ZOOM® Gels.

ProcartaPlex™ Platinum Assay Buffer for Plasma Samples Invitrogen™

ProcartaPlex Platinum Assay Buffer for Plasma Samples is used for dilution of plasma samples and gives spike- and dilution-recovery results in the range of 70–130%.

ProcartaPlex Platinum Assay Buffer for Plasma Samples is included in our Platinum ProcartaPlex Human Basic Kit for Plasma Samples (Cat.No. EPXP010-10421-901) and off-the-shelf Platinum panels. It is offered here as a stand-alone item.

20X Bolt™ MOPS SDS Running Buffer Invitrogen™

Bolt MOPS SDS Running Buffer (20X) is formulated for running proteins on Bolt Bis-Tris Plus gels. It is recommended for separating medium- to large-sized proteins.

Use the right buffer to optimize protein separations
Bolt MES SDS Running Buffer and Bolt MOPS SDS Running Buffer can both be used with Bolt Bis-Tris Plus gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Compare protein migration patterns using MES and MOPs on Bolt Bis-Tris Plus gels

See all available buffers and reagents available for SDS-PAGE

Antibody Diluent

Ready-to-use diluent with BSA (for stabilizing the antibody) and preservative.

SuperBlock™ (TBS) Blocking Buffer Thermo Scientific™

SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

Compare and view all available blocking buffers

Features of SuperBlock Blocking Buffer:
Blocking time—block membranes in 5 to 10 minutes and ELISA plates in 2 minutes
Applications—ELISA, western blotting, IHC; biotin-free for use with streptavidin system
Stability—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Related products
SuperBlock (PBS) Blocking Buffer
SuperBlock T20 (PBS) Blocking Buffer
SuperBlock T20 (TBS) Blocking Buffer
SuperBlock (TBS) Blocking Buffer Dry Blend

SuperBlock™ T20 (TBS) Blocking Buffer Thermo Scientific™

SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

Compare and view all available blocking buffers

Features of SuperBlock Blocking Buffer:
Blocking time—block membranes in 5 to 10 minutes and ELISA plates in 2 minutes
Applications—ELISA, western blotting, IHC; biotin-free for use with streptavidin system
Stability—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Related products
SuperBlock (PBS) Blocking Buffer
SuperBlock T20 (PBS) Blocking Buffer
SuperBlock (TBS) Blocking Buffer
SuperBlock (TBS) Blocking Buffer Dry Blend

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