Shop All Nucleic Acid Molecular Weight Markers

NoLimits 25 bp DNA Fragment Thermo Scientific™

Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.Plasmid DNA, digested with Thermo Scientific Restriction Enzymes, is chromatography-purified to prepare the individual DNA fragments from the digestion mixture. The results are extremely pure DNA fragments, free of any truncated or degraded molecules. These custom DNA fragments are the best custom standards for applications ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Features

• Broad range: 10-20,000 bp
• Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis
• Precise DNA concentration
• Chromatography-purified individual DNA fragments

Applications

• Gel electrophoresis
• Capillary electrophoresis
• DNA quantification
• HPLC analysis
• Applications where a custom DNA size standard is required.

Lambda DNA/EcoRI plus HindIII Marker Thermo Scientific™

Thermo Scientific Lambda DNA/EcoRI+HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

Lambda DNA/EcoRI+HindIII Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, and it can be labeled radioactively or non-radioactively with Klenow fragment or Klenow Fragment, exo- with the fill-in reaction (see Resources for Protocols).

Highlights

• Sizing and approximate quantification of large DNA fragments
• Sharp bands
• Supplied with loading dye for sample DNA

Note
The cohesive ends (the 12nt cos site of lambda DNA) of fragments indicated may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5minutes and then cooling on ice for 3minutes.

The shortest fragments (oblique) are not visible in standard electrophoresis. Fragment lengths are predicted by computer analysis of respective DNA sequences.

GeneRuler 100 bp Plus DNA Ladder, ready-to-use Thermo Scientific™

Thermo Scientific GeneRuler 100 bp Plus DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 100 bp to 3,000 bp on agarose or polyacrylamide gels. The DNA ladder consists of 14 DNA fragments and is provided with 6X TriTrack DNA Loading Dye.

Highlights of 100 bp Plus DNA Ladder
Easy visualization—three-color tracking of DNA migration during electrophoresis with 6X TriTrack DNA Loading Dye (contains Xylene Cyanol FF, Bromophenol Blue, and Orange G)
Simplicity—one loading dye for a variety of samples
Sharp bands—clear results with mixtures of individual chromatography-purified DNA fragments
Convenient—ready-to-load DNA Ladder is premixed with 6X TriTrack DNA Loading dye and stable at room temperature for up to 6 months

Product use
This double-stranded DNA ladder is compatible with 1–2% standard and precast agarose slab gels and can be visualized after ethidium bromide or SYBR Safe staining. A uniform intensity of bands and bright reference bands allow easy sizing and orientation. A defined amount of DNA in each band enables approximate quantification of sample DNA.

NoLimits 6000 bp DNA Fragment Thermo Scientific™

Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.Plasmid DNA, digested with Thermo Scientific Restriction Enzymes, is chromatography-purified to prepare the individual DNA fragments from the digestion mixture. The results are extremely pure DNA fragments, free of any truncated or degraded molecules. These custom DNA fragments are the best custom standards for applications ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Features

• Broad range: 10-20,000 bp
• Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis
• Precise DNA concentration
• Chromatography-purified individual DNA fragments

Applications

• Gel electrophoresis
• Capillary electrophoresis
• DNA quantification
• HPLC analysis
• Applications where a custom DNA size standard is required.

High DNA Mass Ladder Invitrogen™

Invitrogen High DNA Mass Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 1,000 bp to 10,000 bp. High DNA Mass Ladder consists of six individual chromatography-purified DNA fragments.

High DNA Mass Ladder is ideal for separation on 0.8–1% agarose gels.

Highlights of High DNA Mass Ladder:
Sharp, clear bands—chromatography purified fragments for consistent and reliable results
Convenient—provided with 10X BlueJuice Gel Loading Buffer for tracking of sample DNA migration
Precise—an exact amount of DNA in each band

Product use
The double-stranded DNA ladder can be visualized on 0.8–1% agarose gels after ethidium bromide or SYBR Safe staining. The ladder is designed with an equimolar amount of DNA in each band. An exact amount of DNA in each band allows approximate quantification of DNA samples.

MassRuler Express LR Forward DNA Ladder, ready-to-use Thermo Scientific™

Thermo Scientific MassRuler Express LR Forward and Reverse DNA ladders are specially designed for fast and reliable DNA quantification in short separation distances under various electrophoresis conditions. In the forward versions, the mass of each DNA fragment is directly proportional to the fragment size, whereas in the reverse versions, the mass is inversely proportional to fragment's size.

The intensity of the fragment in each ladder is calibrated against a standard that guarantees the precise quantity of each band. The ladders are mixtures of chromatography-purified, individual DNA fragments.

Highlights

• Accurate DNA sizing and quantification—DNA ladder fragments are quantified spectrophotometrically
• Fast and reliable DNA quantification—short separation distances under various electrophoresis conditions
• Sharp bands
• Easy-to-remember fragment sizes and quantities

NoLimits 17000 bp DNA Fragment Thermo Scientific™

Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.Plasmid DNA, digested with Thermo Scientific Restriction Enzymes, is chromatography-purified to prepare the individual DNA fragments from the digestion mixture. The results are extremely pure DNA fragments, free of any truncated or degraded molecules. These custom DNA fragments are the best custom standards for applications ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Features

• Broad range: 10-20,000 bp
• Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis
• Precise DNA concentration
• Chromatography-purified individual DNA fragments

Applications

• Gel electrophoresis
• Capillary electrophoresis
• DNA quantification
• HPLC analysis
• Applications where a custom DNA size standard is required.

NoLimits 800 bp DNA Fragment Thermo Scientific™

Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.Plasmid DNA, digested with Thermo Scientific Restriction Enzymes, is chromatography-purified to prepare the individual DNA fragments from the digestion mixture. The results are extremely pure DNA fragments, free of any truncated or degraded molecules. These custom DNA fragments are the best custom standards for applications ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Features

• Broad range: 10-20,000 bp
• Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis
• Precise DNA concentration
• Chromatography-purified individual DNA fragments

Applications

• Gel electrophoresis
• Capillary electrophoresis
• DNA quantification
• HPLC analysis
• Applications where a custom DNA size standard is required.

Decade™ Markers System (to make radiolabeled RNA markers) Invitrogen™

The Ambion® Decade™ Marker System produces a set of radiolabeled RNA molecules of 150, 100, 90, 80, 70, 60, 50, 40, 30, 20, and 10 nucleotides. The molecular weight markers are ideally suited for experiments where RNA molecules less than 150 nucleotides in length are being studied. Sufficient reagents are provided for 10 marker preparation reactions. This marker set is particularly useful for research laboratories studying tRNA, snRNA, snoRNA, miRNA, siRNA and other small RNA molecules. The system includes all of the reagents necessary to produce the molecular weight markers except gamma-32P-ATP. The Decade™ Markers derive from a single, gel-purified transcript that is provided. The transcript is 5' end-labeled using the kinase and kinase buffer supplied with the kit. The kinase reaction is then diluted into a cleavage reagent that generates the molecular weight marker set in a five minute, room temperature reaction. Gel loading buffer from the kit is then added and the marker is ready for electrophoresis. The labeling, cleavage, and gel preparation steps can be completed in less than an hour.

GeneRuler DNA Ladder Mix Thermo Scientific™

Thermo Scientific GeneRuler DNA Ladder Mix is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 100 bp to 10,000 bp on agarose gels. The DNA ladder consists of 21 DNA fragments and is provided with 6X TriTrack DNA Loading Dye.

Highlights of DNA Ladder Mix
Easy visualization—three-color tracking of DNA migration during electrophoresis with 6X TriTrack DNA Loading Dye (contains Xylene Cyanol FF, Bromophenol Blue, and Orange G)
Simplicity—one loading dye for a variety of samples
Sharp bands—clear results with mixtures of individual chromatography-purified DNA fragments

Product use
This double-stranded DNA ladder is compatible with 1% standard and precast agarose slab gels and can be visualized after ethidium bromide or SYBR Safe staining. A uniform intensity of bands and bright reference bands allow easy sizing and orientation. A defined amount of DNA in each band enables approximate quantification of sample DNA.

O’RangeRuler 100 bp DNA Ladder, ready-to-use Thermo Scientific™

Thermo Scientific O'RangeRuler 100 bp DNA Ladder 100-1500 bp is designed for precise sizing of PCR products and other double-stranded DNA fragments in agarose or non-denaturing polyacrylamide gels. It is step ladder with 100 bp differences between the DNA fragments. It consists of purified and ligated blunt ended basic unit repeats of 100 bp. Not recommended for DNA quantification.

O'RangeRuler 100 bp DNA Ladder is supplied with 6X Orange DNA Loading Dye for sample DNA.

Highlights

• Step ladder with 100 bp increments
• Sharp bands, easy-to-remember fragment sizes
• Evenly spaced reference bands
• Ready-to-use—premixed with 6X Orange Loading Dye for direct loading and room temperature storage
• Supplied with loading dye for sample DNA

NoLimits 8000 bp DNA Fragment Thermo Scientific™

Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.Plasmid DNA, digested with Thermo Scientific Restriction Enzymes, is chromatography-purified to prepare the individual DNA fragments from the digestion mixture. The results are extremely pure DNA fragments, free of any truncated or degraded molecules. These custom DNA fragments are the best custom standards for applications ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Features

• Broad range: 10-20,000 bp
• Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis
• Precise DNA concentration
• Chromatography-purified individual DNA fragments

Applications

• Gel electrophoresis
• Capillary electrophoresis
• DNA quantification
• HPLC analysis
• Applications where a custom DNA size standard is required.

MassRuler Express Forward DNA Ladder Mix, ready-to-use Thermo Scientific™

Thermo Scientific MassRuler Express Forward and Reverse DNA Ladder mixes are specially designed for fast and reliable DNA quantification in short separation distances under various electrophoresis conditions (see Table 1). In the forward versions, the mass of each DNA fragment is directly proportional to the fragment size whereas, in the reverse versions, the mass is inversely proportional to fragment's size.

The intensity of the fragment in each ladder is calibrated against a standard that guarantees the precise quantity of each band. The ladders are mixtures of chromatography-purified, individual DNA fragments.

Highlights
• Accurate DNA sizing and quantification—DNA ladder fragments are quantified spectrophotometrically
• Fast and reliable DNA quantification &mdash short separation distances under various electrophoresis conditions
• Sharp bands
• Easy-to-remember fragment sizes and quantities
• Ready-to-use—premixed with 6X MassRuler DNA Loading Dye for direct loading and room temperature storage
• Supplied with loading dye for sample DNA

NoLimits 400 bp DNA Fragment Thermo Scientific™

Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.Plasmid DNA, digested with Thermo Scientific Restriction Enzymes, is chromatography-purified to prepare the individual DNA fragments from the digestion mixture. The results are extremely pure DNA fragments, free of any truncated or degraded molecules. These custom DNA fragments are the best custom standards for applications ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Features

• Broad range: 10-20,000 bp
• Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis
• Precise DNA concentration
• Chromatography-purified individual DNA fragments

Applications

• Gel electrophoresis
• Capillary electrophoresis
• DNA quantification
• HPLC analysis
• Applications where a custom DNA size standard is required.

RiboRuler High Range RNA Ladder Thermo Scientific™

Thermo Scientific RiboRuler High Range RNA Ladder 200 to 6000 bases is a mixture of 8 chromatography purified single-stranded RNA transcripts (200 to 6000 bp long), produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments.

The RiboRuler High Range RNA Ladder is available in 1 mM EDTA, pH 6.0.

RiboRuler High Range RNA Ladder is composed of chromatography-purified transcripts that makes them free from NTPs and RNA degradation products. The concentration of each RNA transcript is determined spectrophotometrically. To minimize the possibility of contamination during the use, the ladder is aliquotted in smaller volumes.

RiboRuler High Range RNA Ladder is optimal for end-labeling using T4 Polynucleotide Kinase (see protocol in the Resources) and is ideal for Northern blots.

Highlights

• Sharp bands of uniform intensity
• Easy-to-remember band sizes and quantities
• Predetermined concentration of ladder bands allows for approximate RNA quantification
• Supplied with 2X RNA Loading Dye
• Stable for 6 months at -20°C

Applications

• RNA sizing and quantification on native or denaturing gels
• Northern blotting
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