Shop All Nucleic Acid Molecular Weight Markers

TrackIt™ 100 bp DNA Ladder Invitrogen™

Invitrogen TrackIt 100 bp DNA Ladder is ready to use and designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 2,000 bp. 100 bp DNA Ladder consists of 13 individual chromatography-purified DNA fragments and has reference bands at 2000, 1500, and 600 bp for easy orientation.

TrackIt 100 bp DNA Ladder is ideal for separation on 1–2% agarose gels.

Highlights of TrackIt 100 bp DNA Ladder:
Sharp, clear bands—chromatography purified fragments for consistent and reliable results and stable at room temperature up to six months
Ready-to-use—premixed with 6X TrackIt Cyan/Orange Loading Buffer for tracking of DNA migration
Precise—an exact amount of DNA in each band

Product use
The double-stranded DNA ladder can be visualized on 1–2% agarose gels after ethidium bromide or SYBR Safe staining. The ladder is designed with a uniform intensity of DNA bands for a clear view of each band. An exact amount of DNA in each band allows approximate quantification of DNA samples.

NoLimits 10000 bp DNA Fragment Thermo Scientific™

Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.Plasmid DNA, digested with Thermo Scientific Restriction Enzymes, is chromatography-purified to prepare the individual DNA fragments from the digestion mixture. The results are extremely pure DNA fragments, free of any truncated or degraded molecules. These custom DNA fragments are the best custom standards for applications ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Features

• Broad range: 10-20,000 bp
• Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis
• Precise DNA concentration
• Chromatography-purified individual DNA fragments

Applications

• Gel electrophoresis
• Capillary electrophoresis
• DNA quantification
• HPLC analysis
• Applications where a custom DNA size standard is required.

TrackIt™ 1 Kb Plus DNA Ladder Invitrogen™

Invitrogen TrackIt 1 Kb Plus DNA Ladder is ready to use and designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 15,000 bp. 1 Kb Plus DNA Ladder consists of 18 individual chromatography-purified DNA fragments and has a reference band at 1,500 bp for easy orientation.

TrackIt 1 Kb DNA Ladder is ideal for separation on 0.8–1% agarose gels.

Highlights of TrackIt 1 Kb Plus DNA Ladder:
Sharp, clear bands—chromatography purified fragments for consistent and reliable results and stable at room temperature up to six months
Ready-to-use—premixed with 6X TrackIt Cyan/Orange Loading Buffer for tracking of DNA migration
Precise—an exact amount of DNA in each band

Product use
The double-stranded DNA ladder can be visualized on 0.8–1% agarose gels after ethidium bromide or SYBR Safe staining. The ladder is designed with a uniform intensity of DNA bands for a clear view of each band. An exact amount of DNA in each band allows approximate quantification of DNA samples.

NoLimits 25 bp DNA Fragment Thermo Scientific™

Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.Plasmid DNA, digested with Thermo Scientific Restriction Enzymes, is chromatography-purified to prepare the individual DNA fragments from the digestion mixture. The results are extremely pure DNA fragments, free of any truncated or degraded molecules. These custom DNA fragments are the best custom standards for applications ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Features

• Broad range: 10-20,000 bp
• Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis
• Precise DNA concentration
• Chromatography-purified individual DNA fragments

Applications

• Gel electrophoresis
• Capillary electrophoresis
• DNA quantification
• HPLC analysis
• Applications where a custom DNA size standard is required.

TrackIt™ Cyan/Orange Loading Buffer Invitrogen™

TrackIt loading buffers are 6X solutions used for easy loading and tracking of DNA samples in agarose gels. They contain two tracking dyes: one that runs behind the sample and one that runs ahead of the sample. The tracking dyes serve as visual markers of migration during electrophoresis and indicate when maximum resolution of the DNA fragments has been achieved. Additionally, visualization of DNA bands is not obscured by the tracking dyes because they run outside the limits of most DNA samples.TrackIt Cyan/Orange Loading Buffer is recommended for DNA fragments between 50 bp and 10 Kb.TrackIt Cyan/Yellow Loading Buffer is recommended for DNA fragments between 10 bp and 1 Kb.

E-Gel™ 1 Kb Plus DNA Ladder Invitrogen™

Invitrogen E-Gel 1 Kb Plus DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 15,000 bp. E-Gel 1 Kb Plus DNA Ladder consists of 18 individual chromatography-purified DNA fragments and has a reference band at 1,500 bp for easy orientation.

E-Gel 1 Kb Plus DNA Ladder is specifically formulated for optimal performance on pre-cast E-Gel agarose gels.

Highlights of E-Gel 1 Kb Plus DNA Ladder:
Performance—optimal for use on E-Gel agarose gels
Sharp, clear bands—chromatography purified fragments for consistent and reliable results and stable at room temperature up to six months
Ready-to-use—premixed with loading buffer
Convenient—provided with 1X E-Gel Sample Loading Buffer
Precise—an exact amount of DNA in each band

Product use
The double-stranded DNA ladder can be visualized on 0.8–1% E-Gel agarose gels. The ladder is designed with a uniform intensity of DNA bands for a clear view of each band. An exact amount of DNA in each band allows approximate quantification of DNA samples.

NoLimits 17000 bp DNA Fragment Thermo Scientific™

Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.Plasmid DNA, digested with Thermo Scientific Restriction Enzymes, is chromatography-purified to prepare the individual DNA fragments from the digestion mixture. The results are extremely pure DNA fragments, free of any truncated or degraded molecules. These custom DNA fragments are the best custom standards for applications ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Features

• Broad range: 10-20,000 bp
• Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis
• Precise DNA concentration
• Chromatography-purified individual DNA fragments

Applications

• Gel electrophoresis
• Capillary electrophoresis
• DNA quantification
• HPLC analysis
• Applications where a custom DNA size standard is required.

TrackIt™ Cyan/Yellow Loading Buffer Invitrogen™

TrackIt loading buffers are 6X solutions used for easy loading and tracking of DNA samples in agarose gels. They contain two tracking dyes: one that runs behind the sample and one that runs ahead of the sample. The tracking dyes serve as visual markers of migration during electrophoresis and indicate when maximum resolution of the DNA fragments has been achieved. Additionally, visualization of DNA bands is not obscured by the tracking dyes because they run outside the limits of most DNA samples.TrackIt Cyan/Orange Loading Buffer is recommended for DNA fragments between 50 bp and 10 Kb.TrackIt Cyan/Yellow Loading Buffer is recommended for DNA fragments between 10 bp and 1 Kb.

NoLimits 800 bp DNA Fragment Thermo Scientific™

Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.Plasmid DNA, digested with Thermo Scientific Restriction Enzymes, is chromatography-purified to prepare the individual DNA fragments from the digestion mixture. The results are extremely pure DNA fragments, free of any truncated or degraded molecules. These custom DNA fragments are the best custom standards for applications ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Features

• Broad range: 10-20,000 bp
• Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis
• Precise DNA concentration
• Chromatography-purified individual DNA fragments

Applications

• Gel electrophoresis
• Capillary electrophoresis
• DNA quantification
• HPLC analysis
• Applications where a custom DNA size standard is required.

Millennium™ RNA Markers-Formamide Invitrogen™

The Ambion® Millennium™ Markers-Formamide are designed to provide accurate size determination of single-stranded RNA transcripts from 0.5 to 9 kb and can be used in any Northern protocol. Sufficient markers are provided for 25 Northern gel lanes. They are a mixture of 10 discrete RNA transcripts with easy-to-remember sizes: 0.5, 1, 1.5, 2, 2.5, 3, 4, 5, 6, and 9 kilobases. The markers are supplied in formamide and show increased stability and resistance to RNase compared to other large molecular weight RNA markers. They may be stained with ethidium bromide either during or after electrophoresis. Because of their quantity and even spacing, these markers are ideal for constructing very accurate standard curves for mRNA size determination. They also provide a reference scale for easy verification and comparison of known bands. 1-2 µg (1-2 µl) of RNA Millennium™ Markers will generate 10 distinct bands on a 1% denaturing agarose gel with ethidium bromide staining. All marker lots undergo stringent nuclease testing and are shown to be stable overnight at 37°C.

E-Gel™ 1 Kb Plus Express DNA Ladder Invitrogen™

Invitrogen E-Gel 1 Kb Plus Express DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 5,000 bp. E-Gel 1 Kb Plus Express DNA Ladder consists of nine individual chromatography-purified DNA fragments with reference bands at 1,500 and 500 bp for easy orientation.

E-Gel 1 Kb Plus Express DNA Ladder is specifically formulated for optimal performance on pre-cast E-Gel agarose gels.

Highlights of E-Gel 1 Kb Plus Express DNA Ladder:
Performance—optimized for use on E-Gel CloneWell ll agarose gels
Sharp, clear bands—chromatography-purified fragments for consistent and reliable results, stable at room temperature up to six months
Ready to use—premixed with loading buffer
Convenient—provided with 1X E-Gel Sample Loading Buffer
Precise—an exact amount of DNA in each band

Product use
The double-stranded DNA ladder can be visualized on 0.8% E-Gel CloneWell II and E-Gel EX agarose gels. The ladder is designed with a uniform intensity of DNA bands for a clear view of each band. An exact amount of DNA in each band allows approximate quantification of DNA samples.

Lambda DNA/HindIII Marker Thermo Scientific™

Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

The marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, and it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction (see Resources for Protocols).

Highlights

• Sizing and approximate quantification of large DNA fragments
• Sharp bands
• Supplied with loading dye for sample DNA

Note
The cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 minutes and then cooling on ice for 3 minutes.

GeneRuler 1 kb Plus DNA Ladder, ready-to-use Thermo Scientific™

Thermo Scientific GeneRuler 1 kb Plus DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 75 bp to 20,000 bp on agarose gels. The DNA ladder consists of 15 DNA fragments and is provided with 6X TriTrack DNA Loading Dye.

Highlights of 1 kb Plus DNA Ladder
Easy visualization—three-color tracking of DNA migration during electrophoresis with 6X TriTrack DNA Loading Dye (contains Xylene Cyanol FF, Bromophenol Blue, and Orange G)
Simplicity—one loading dye for a variety of samples
Sharp bands—clear results with mixtures of individual chromatography-purified DNA fragments
Convenient—ready-to-load DNA Ladder is premixed with 6X TriTrack DNA Loading dye and stable at room temperature for up to 6 months

Product use
This double-stranded DNA ladder is compatible with 1% standard and precast agarose slab gels and can be visualized after ethidium bromide or SYBR Safe staining. A uniform intensity of bands and bright reference bands allow easy sizing and orientation. A defined amount of DNA in each band enables approximate quantification of sample DNA.

RiboRuler High Range RNA Ladder Thermo Scientific™

Thermo Scientific RiboRuler High Range RNA Ladder 200 to 6000 bases is a mixture of 8 chromatography purified single-stranded RNA transcripts (200 to 6000 bp long), produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments.

The RiboRuler High Range RNA Ladder is available in 1 mM EDTA, pH 6.0.

RiboRuler High Range RNA Ladder is composed of chromatography-purified transcripts that makes them free from NTPs and RNA degradation products. The concentration of each RNA transcript is determined spectrophotometrically. To minimize the possibility of contamination during the use, the ladder is aliquotted in smaller volumes.

RiboRuler High Range RNA Ladder is optimal for end-labeling using T4 Polynucleotide Kinase (see protocol in the Resources) and is ideal for Northern blots.

Highlights

• Sharp bands of uniform intensity
• Easy-to-remember band sizes and quantities
• Predetermined concentration of ladder bands allows for approximate RNA quantification
• Supplied with 2X RNA Loading Dye
• Stable for 6 months at -20°C

Applications

• RNA sizing and quantification on native or denaturing gels
• Northern blotting

E-Gel™ Sample Loading Buffer, 1X Invitrogen™

E-Gel Sample Loading Buffer is supplied as a ready-to-use 1X solution, and is formulated specifically for maximum performance on E-Gel EX gels as well as other E-Gel precast gels.
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