Western Blot Substrates and Substrate Kits

Western blot substrates and substrate kits are suitable for western blot detection of alkaline phosphatase (AP) and horseradish peroxidase (HRP) enzyme conjugates. These products may be chemiluminescent or chromogenic precipitation substrates, and are available in a spectrum of low- to high-sensitivity reagents.
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SuperSignal™ West Pico PLUS Chemiluminescent Substrate Thermo Scientific™

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

1-Step™ Chloronaphthol Substrate Solution Thermo Scientific™

Thermo Scientific Pierce 1-Step Chloronaphthol Solution is a solution of 4-chloro-1-naphthol for the chromogenic detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods. Chloronaphthol (4-CN, MW = 178.

1-Step™ NBT/BCIP plus Suppressor Substrate Solution Thermo Scientific™

Thermo Scientific Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.

NBT Substrate Powder (nitro-blue tetrazolium chloride) Thermo Scientific™

Thermo Scientific Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.

SuperSignal™ West Femto Maximum Sensitivity Substrate Thermo Scientific™

Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme.

Pierce™ CN/DAB Substrate Kit Thermo Scientific™

Thermo Scientific Pierce CN/DAB Substrate Kit includes substrate and peroxide solutions for combined chloronaphthol- and diaminobenzidine-based detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.

1-Step™ NBT/BCIP Substrate Solution Thermo Scientific™

Thermo Scientific Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.

SuperSignal™ West Dura Extended Duration Substrate Thermo Scientific™

Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

SuperSignal™ Western Blot Substrate Bundle, Pico PLUS + trial-size Femto Thermo Scientific™

This substrate kit is comprised of SuperSignal West Pico PLUS Chemiluminescent Substrate plus a sample size of SuperSignal West Femto Maximum Sensitivity Substrate to provide chemiluminescent detection substrates for both routine and high sensitivity western blotting.

SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto Thermo Scientific™

This substrate bundle contains SuperSignal West Femto Maximum Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate to enable trial and comparison of the newest and most sensitive substrate for low abundant targets.

1-Step™ Ultra TMB-Blotting Solution Thermo Scientific™

Thermo Scientific 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting. This precipitating, colorimetric Western blot substrate for HRP provides high sensitivity, increased signal-to-noise ratio and low background compared to...

Pierce™ ECL Western Blotting Substrate Thermo Scientific™

Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Pierce™ DAB Substrate Kit Thermo Scientific™

The Thermo Scientific Pierce DAB Substrate Kit enables chromogenic detection of horseradish peroxidase (HRP) activity based the action of 3,3'-diaminobenzidine (DAB) in Western blot and tissue staining methods. DAB (3,3'-diaminobenzidine tetrahydrochloride; MW = 214.

1-Step™ TMB-Blotting Substrate Solution Thermo Scientific™

Thermo Scientific Pierce TMB-Blotting 1-Step Solution is a ready-to-use solution of the blue TMB peroxidase (HRP) substrate that is specially formulated for chromogenic detection in Western blot and IHC experiments. TMB (3,3',5,5'-tetramethylbenzidine; MW = 240.

BCIP Substrate Powder (5-bromo-4-chloro-3'-indolylphosphate p-toluidine) Thermo Scientific™

Thermo Scientific Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolylphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
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