Shop All Western Blot Equipment and Supplies

BenchPro™ 4100 Reagent Vials Invitrogen™

BenchPro® 4100 Reagent Vials are available as a package of 50- 25ml vials. They are compatible for use with the BenchPro® 4100 Card Processing Station.

iBlot™ 2 Transfer Stacks, nitrocellulose, regular size Invitrogen™

iBlot 2 Transfer Stacks are used to transfer proteins using the iBlot 2 Gel Transfer Device. They are consumable stacks with an integrated nitrocellulose transfer membrane for dry blotting of proteins. Each iBlot 2 Transfer Stack contains a copper-coated electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins. This regular-size stack (13 cm x 8.3 cm) can be used with midi-size gels or two mini-size gels or E-PAGE 48 or 96 gels.

Learn more about the iBlot 2 system ›

Nitrocellulose membrane
The nitrocellulose membrane (0.2-µm pore size) in this stack is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. The proteins bind to the membrane due to hydrophobic and electrostatic interactions. The protein-binding capacity is 209 µg/cm2.

iBlot™ Transfer Stack, nitrocellulose, mini Invitrogen™

iBlot® Transfer Stacks are used to transfer proteins using the iBlot® Gel Transfer Device. The iBlot® Transfer Stacks are disposable stacks that come in three varieties for different applications. iBlot® Gel Transfer Stacks have integrated PVDF or nitrocellulose transfer membranes to perform dry blotting of proteins. iBlot® DNA Transfer Stacks have an integrated nylon transfer membrane to perform dry blotting of DNA. iBlot® Western Detection Stacks are used to perform western detection on previously blotted nitrocellulose or PVDF membranes. Each iBlot® Transfer Stack contains a copper electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins or DNA.

Nitrocellulose membrane
The nitrocellulose membrane (0.2 µm pore size) is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, or radiolabeling. Proteins bind to the membrane due to hydrophobic and electrostatic interactions. The protein binding capacity is 209 µg/cm2.

Transfer stack sizes
The regular size (13 cm x 8.3 cm) is used for all Novex® midi-gel types (or equivalent) and E-PAGE™ 48 or 96 Gels. The mini size (8 cm x 8 cm) is used for all Novex® mini-gels (or equivalent).

Novex™ Semi-Dry Blotter Invitrogen™

The Novex® Semi-Dry Blotter quickly and efficiently transfers proteins and nucleic acids from mini and midi gels to membranes. Simultaneous transfer can be achieved from up to 2 midi gels, 4 mini gels, or 2 E-PAGE™ gels. The Novex® Semi-Dry Blotter is designed for fast set-up and ease of use. The platinum-coated titanium anode plate and stainless steel cathode plate provide fast and efficient electroblotting using less transfer buffer than wet (tank) blotting methods.

The Novex® Semi-Dry Blotter is designed for convenience and efficiency:
• Economical – Horizontal configuration requires less than 200 ml of buffer to transfer
• High-capacity – A large, 20 x 20 cm transfer surface allows up to two midi gels or four mini gels to be transferred at one time
• Flexible – Field strength across the blot is maximized and evenly distributed, for efficient and complete transfers regardless of the number and size of gels blotted
• Durable – Stainless steel and platinum-coated titanium electrodes allow for corrosion-free operation
• Fast – Blotting is typically completed in 30-60 min

A Complete Western Blotting System
We offer precut (8.3 cm × 7.3 cm) and pre-assembled membranes and filter paper sandwiches (available separately) to further simplify and speed the western blotting process. Choose between Invitrolon™ PVDF (LC2002 or LC2005), or nitrocellulose (LC2000 or LC2001) containing sandwiches.

The Novex® Semi-Dry Blotter requires an external power supply for operation (up to 800 mA, depending on the number and size of blots).

iBlot™ 2 Transfer Stacks, nitrocellulose, mini Invitrogen™

iBlot 2 Transfer Stacks are used to transfer proteins using the iBlot 2 Gel Transfer Device. They are consumable stacks with an integrated nitrocellulose transfer membrane for dry blotting of proteins. Each iBlot 2 Transfer Stack contains a copper-coated electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins. This mini-size stack (8 cm x 8 cm) can be used with one mini-size gel.

Learn more about the iBlot 2 system ›

Nitrocellulose membrane
The nitrocellulose membrane (0.2-µm pore size) in this stack is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. The proteins bind to the membrane due to hydrophobic and electrostatic interactions. The protein-binding capacity is 209 µg/cm2.

Bandmate™ Funnel Tray Invitrogen™

The Bandmate Funnel Tray is a replacement tray specially designed for use with the Bandmate Automated Western Blot Processor. This funnel tray is required for the funneling of antibody solution to antibody recovery tubes and for the directing of used buffer to waste trays during western blot processing.

Learn more about the Bandmate Automated Western Blot Processor ›

iBind™ Flex Western Starter Kit, fluorescent detection Invitrogen™

The iBind Flex Western Starter Kit for fluorescent detection includes an iBind Flex Western Device , iBind Flex Cards, and an iBind Flex Fluorescent Detection (FD) Solution Kit . The iBind Flex Western Device is a small benchtop device that automates western blot processing (immunodetection). It applies mechanical pressure to an iBind Flex Card to generate sequential lateral flow (SLF) of immunodetection solutions for the blocking, antibody binding, and washing steps. The glass fiber matrix of the iBind Flex Card allows for homogenous and consistent flow of the solutions over the membrane, increasing the antigen-antibody interaction.

The iBind Flex Western Device allows all solutions to be prepared and loaded in the device at the start of the procedure; all subsequent steps proceed automatically through SLF action—no electricity or batteries required. Blots are ready for the final detection steps in approximately 2.5 hours.

Learn more about iBind western systems ›

The iBind Flex Western Device includes three user-interchangeable well inserts to process up to one midi blot, two mini blots, or six vertically-cut strip blots when used with iBind Flex Cards, and the iBind Flex Solution Kit or iBind Flex Fluorescent Detection (FD) Solution Kit to process one mini-blot.

iBlot™ Transfer Stack, PVDF, mini Invitrogen™

The iBlot® Transfer Stacks are used to transfer proteins using the iBlot® Gel Transfer Device. The iBlot® Transfer Stacks are disposable stacks that come in three varieties for different applications: iBlot® Gel Transfer Stacks have integrated PVDF or nitrocellulose transfer membranes to perform dry blotting of proteins, iBlot® DNA Transfer Stacks have an integrated nylon transfer membrane to perform dry blotting of DNA, and iBlot® Western Detection Stacks are used to perform Western detection on previously blotted nitrocellulose or PVDF membranes. Each iBlot® Transfer Stack contains a copper electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins or DNA.

PVDF membrane
The PVDF membrane (0.2 µm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. The PVDF membrane is preactivated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, or radiolabeling. The proteins bind to the membrane due to hydrophobic interactions. The protein binding capacity is 240 µg/cm2.

Transfer stack sizes
The regular size (13 cm x 8.3 cm) is used for all Novex® midi-gel types (or equivalent) and E-PAGE™ 48 or 96 Gels. The mini size (8 cm x 8 cm) is used for all Novex® mini-gels (or equivalent).

Power Blotter Select Transfer Stacks, nitrocellulose, regular size Invitrogen™

The Invitrogen Power Blotter Select Transfer Stacks are used to transfer proteins using the Power Blotter System. They are disposable, pre-assembled stacks that come with an integrated nitrocellulose transfer membrane for the dry blotting of proteins from polyacrylamide gels. Each Power Blotter Select Transfer Stack contains filter paper, membrane, and the appropriate cathode and anode buffers incorporated into a solid gel matrix to allow fast, reliable transfer of proteins.

Features of the Power Blotter Select Transfer Stacks:
Rapid transfer—the ready-to-use transfer stack takes little setup time and protein transfers take place in as little as seven minutes
Efficient and even transfer—stacks are made with high quality membranes that provide high binding efficiency, uniform transfer, and high detection sensitivity
Increased reproducibility—preassembled stacks provide consistent performance

Learn more about the use of the Power Blotter Select Transfer Stacks in Power Blotter System

Nitrocellulose membrane
The nitrocellulose membrane (0.2 µm pore size) in the stack is composed of 100% pure nitrocellulose to provide high-quality transfer. The membrane is compatible with commonly used detection methods such as staining, chemiluminescence, and fluorescence. The proteins bind to the membrane through hydrophobic and electrostatic interactions. The protein binding capacity is 209 µg/cm2.

Regular-size transfer stacks
Regular-size Power Blotter Select Stacks (13 cm x 8.3 cm) can be used with all Invitrogen Novex and NuPAGE midi-gels (or equivalent) or two mini-size gels (all Novex, Bolt, and NuPAGE mini-size gels, or equivalent).

Related products
Power Blotter Select Transfer Stacks, nitrocellulose, mini size
Power Blotter Select Transfer Stacks, PVDF, mini
Power Blotter Select Transfer Stacks, PVDF, regular size

iBlot™ Transfer Stack, PVDF, regular size Invitrogen™

The iBlot® Transfer Stacks are used to transfer proteins using the iBlot® Gel Transfer Device. The iBlot® Transfer Stacks are disposable stacks that come in three varieties for different applications: iBlot® Gel Transfer Stacks have integrated PVDF or nitrocellulose transfer membranes to perform dry blotting of proteins, iBlot® DNA Transfer Stacks have an integrated nylon transfer membrane to perform dry blotting of DNA, and iBlot® Western Detection Stacks are used to perform Western detection on previously blotted nitrocellulose or PVDF membranes. Each iBlot® Transfer Stack contains a copper electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins or DNA.

PVDF membrane
The PVDF membrane (0.2 µm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. The PVDF membrane is preactivated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, or radiolabeling. The proteins bind to the membrane due to hydrophobic interactions. The protein binding capacity is 240 µg/cm2.

Transfer stack sizes
The regular size (13 cm x 8.3 cm) is used for all Novex® midi-gel types (or equivalent) and E-PAGE™ 48 or 96 Gels. The mini size (8 cm x 8 cm) is used for all Novex® mini-gels (or equivalent).

Owl™ VEP-2 Mini Tank Electroblotting System Thermo Scientific™

Produce uniform, reproducible protein transfers for up to four polyacrylamide minigels with the Thermo Scientific™ Owl™ VEP-2 Mini Tank Electroblotting System, ideal for fast, efficient transfer of proteins over a wide molecular weight range.

iBright™ SAE Software for 21 CFR Part 11, single license Invitrogen™

iBright SAE Software for 21 CFR Part 11 is for use with the iBright Imaging System and supports compliance with 21 CFR Part 11 FDA guidelines for security, auditing, and electronic (SAE) signatures using the SAE console.

Features of the iBright SAE Software include:
Security
• Authentication of user login information
• Ability to set password guidelines and expiry dates
• Lockout access and re-authentication requirements after a period of inactivity
• System lockout upon unauthorized attempts to access software
• Ability of administrator to define user roles and permissions
• Verification of each user's permissions to perform predefined activities
• Identification of data tampering

Auditing
• Generation of audit trail log
• Tracking of changes and committed actions to user roles, account management, and system settings
• Ability of administrator to determine functions required to input reason for commit
• Audit trail record presented in readable and filterable formats

Electronic signature
• Administrator determination of e-signature requirements
• Ability to review records and sign electronically

Along with the 21 CFR part 11 software, our service and support organization offers IQ/OQ qualification services for your instrument and support training including SmartStart services.

iBlot™ 2 Transfer Stacks, PVDF, regular size Invitrogen™

iBlot 2 Transfer Stacks are used to transfer proteins using the iBlot 2 Gel Transfer Device. iBlot 2 Transfer Stacks are consumable stacks with an integrated pre-activated PVDF transfer membrane for dry blotting of proteins. Each iBlot 2 Transfer Stack contains a copper-coated electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins. This regular-size stack (13 cm x 8.3 cm) can be used with midi-size gels or two mini-size gels or E-PAGE 48 or 96 gels.

Learn more about the iBlot 2 system ›

PVDF membrane
The PVDF membrane (0.2-µm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. It is pre-activated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. The proteins bind to the membrane due to hydrophobic interactions. The protein-binding capacity is 240 µg/cm2.

iBlot™ 2 Gel Transfer Device

The iBlot 2 Gel Transfer Device is a dry transfer device that performs western blotting transfer efficiently and reliably, within seven minutes, and without the need for liquid buffers. The iBlot 2 system is compatible with both polyvinylidene difluoride (PVDF) and nitrocellulose membranes and has comparable performance to traditional wet-transfer methods in a fraction of the time. The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF).

Learn more about the iBlot 2 system ›

iBlot 2 Gel Transfer Devivce features include:
• Complete protein transfer in seven minutes or less
• High detection sensitivity and even transfer
• Increased blotting reliability and reproducibility
• A simple, user-friendly system
• Pre-programed with six voltage methods that allows blotting using different combinations of volts and time
• Ability to create custom programs
• Built-in tutorials and application notes
• Transfer proteins from mini-, midi-, and E-PAGE-sized gels

How the iBlot 2 Dry Blotting System works
Buffer ion reservoirs are incorporated into the gel matrix of transfer stacks instead of buffer tanks or soaked papers. The high density of ions in the gel matrix enables rapid protein transfer. During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen). With the iBlot 2 Dry Blotting System, transfer time is reduced by the shortened distance between electrodes, high field strength, and high current. Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to our unique de-bubbling design that promotes even and complete transfer. With the iBlot 2 system, there is no need to prepare buffers, pre-treat your gel, or clean up after blotting. The total preparation and run time is normally less than ten minutes per blot.

Bandmate™ Blot Tray Cover Invitrogen™

The Bandmate Blot Tray Cover is a replacement cover specially designed for use with the Bandmate Automated Western Blot Processor mini and midi incubation trays. This tray cover is required to protect blots and reagents during western blot processing.

Learn more about the Bandmate Automated Western Blot Processor ›

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