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PVDF Transfer Membrane, 0.45 µm, 26.5 cm x 3.75 m Thermo Scientific™

Pierce PVDF Transfer Membrane, 0.45 µm, is made of high-quality polyvinylidene difluoride and provides high binding capacity for proteins and nucleic acids for western, southern, and northern blotting methods. The 0.45-µm pore size makes this membrane ideal for transfer for proteins >10 kDa.

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Features:
Membrane: polyvinylidene difluoride (PVDF)
Binding capacity: goat IgG: 294 µg/cm2; BSA: 215 µg/cm2; insulin: 160 µg/cm2
Re-probe characteristics: yes
Pre-activation: required with 100% alcohol (methanol)
Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, chemiluminescence, and radiolabeling
Durability: compatible with most organic solvents, acids, and mild bases

Nitrocellulose Membranes, 0.45 µm, 8 x 8 cm Thermo Scientific™

Pierce Nitrocellulose Membranes, 0.45 µm, 8 x 8 cm, have excellent binding properties for western blotting, dot-blot assays, and other protein or nucleic acid methods. The 0.45-µm pore size is ideal for the transfer of most proteins (>20 kDa) and nucleic acids (>300 bp).

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Features:
Media: 100% pure nitrocellulose
Binding capacity: ~45-60 µg/cm2 of BSA
Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling

UV Transilluminator Bulb, 365 nm, for myECL™ Imager Thermo Scientific™

This lamp bulb with ultraviolet emission (365nm) is used to replace the pre-installed 306nm UV bulbs in the Thermo Scientific myECL Imager with long-wave 365nm bulbs; all six lamps must be the same bulb type (365nm or 306nm).

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Improved Western blot detection using CCD digital imaging

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Blue Filter for myECL™ Imager Thermo Scientific™

The Thermo Scientific 445-480nm bandpass filter is a threaded, 50mm-diameter glass filter. This product is used for the addition of a blue filter to one of two open positions in the myECL Imager for detection of blue-fluorescent dyes and stains.

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Improved Western blot detection using CCD digital imaging

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myECL™ Imager

Nitrocellulose Membrane, 0.45 μm, 30 cm x 3.5 m Thermo Scientific™

Pierce Nitrocellulose Membrane, 0.45 µm, comes in a convenient 30 cm x 3.5m roll and has excellent binding properties for western blotting, dot-blot assays, and other protein or nucleic acid methods. The 0.45-µm pore size is ideal for the transfer of most proteins (>20 kDa) and nucleic acids (>300 bp).

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Features:
Membrane: 100% pure nitrocellulose
Binding capacity: ~80 µg/cm2 of proteins
Compatibility: compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling
• Economical—30 cm x 3.5 m roll for cutting to any dimensions

Imaging Reference Target for myECL™ Imager Thermo Scientific™

Plastic imaging target with printed design; 7in x 6.5in. This product is a replacement for the myECL Imager accessory used for focusing in all imaging modes.

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Improved Western blot detection using CCD digital imaging

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CL-XPosure™ Film, 7 x 9.5 in. (18 x 24 cm) Thermo Scientific™

Thermo Scientific CL-XPosure Film is an economically priced clear-blue X-ray film especially for detection and documentation of ECL-based Western blots and other chemiluminescent protein or nucleic acid assay methods.

CL-XPosure Film is an excellent phototgraphic film for use with enhanced chemiluminescence (ECL) substrates for horseradish peroxidase (HRP) or alkaline phosphatase (AP). Convenient sizes are offered for protein Western blotting, standard film cassettes and film developing equipment. The autoradiography film also works well for chemiluminescent or isotopic DNA and RNA methods, including Southern and Northern blotting and gel-shift assays (EMSA). The quality and price of this film make it the smart choice for life science research applications.

Features of CL-XPosure Film:

High performance – just as sensitive as other commercially available autoradiography films
Affordable – costs up to 75% less than other brands of comparable quality X-ray film
Convenient – available in five sheet sizes in packages containing 50 or 100 non-interleaved sheets
Perfect match – validated for use with Thermo Scientific SuperSignal Chemiluminescent Substrates and Thermo Scientific Pierce ECL Chemiluminescent Substrate

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CL-XPosure™ Film, 5 x 7 in. (13 x 18 cm)
CL-XPosure™ Film, 8 x 10 in. (20 x 25 cm)
CL-XPosure™ Film, 9.5 x 11.8 in. (24 x 30 cm)
CL-XPosure™ Film, 14 x 17 in. (35 x 43 cm)

UV Transilluminator Bulb, 306 nm, for myECL™ Imager Thermo Scientific™

This lamp bulb with ultraviolet emission (306nm) is used to replace one of six pre-installed 306nm UV bulbs in the Thermo Scientific myECL Imager; all six lamps must be the same bulb type (306nm or 365nm).

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Improved Western blot detection using CCD digital imaging

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myECL™ Imager

Chemiluminescence Exposure Screen for myECL™ Imager Thermo Scientific™

This Thermo Scientific black plastic sample screen is used to cover the transilluminator on the myECL Imager. It is a replacement for the accessory used on the transilluminator for acquiring chemiluminescent images. Measurements: 24.5 cm x 19.5 cm x 3.2mm.

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Improved Western blot detection using CCD digital imaging

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Biodyne™ B Nylon Membrane, 0.45 µm, 8 cm x 12 cm Thermo Scientific™

Thermo Scientific Biodyne B Nylon Membranes are sheets of positively charged nylon that provide excellent binding characteristics for nucleic acid transfer, blotting and detection methods.

Features of Biodyne B Nylon Membranes:

Versatile and durable—achieve strong DNA binding by baking or UV-crosslinking without damaging the membrane
Easy to use—pre-cut 8 x 12 cm sheets are the ideal size for standard mini-gel transfers and microplate dot-blots
High signal-to-noise ratio—lower background than nitrocellulose and most other nylon transfer membranes

Biodyne B Nylon Membrane is manufactured with a matrix pore size of 0.4 micron, and is supplied in packages of 8 x 12 cm sheets. Biodyne B Membrane contains exclusively positive charges, providing the highest possible binding capacity for negatively charged molecules like DNA and RNA. The membrane is resistant to heat and solvents and will not shrink, crack or tear.

Properties of Biodyne B Nylon Membrane:
• Composition: positively charged Nylon 66
• Surface: strong cationic quaternary ammonium groups provide positive zeta potential, even to pH 10
• Properties: excellent for DNA, RNA and other negatively charged molecules
• Applications: Southern blotting, Northern blotting, DNA and RNA methods, including gel shift assays with Thermo Scientific LightShift Chemiluminescent EMSA Kits

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Transfer EMSA gels using the Pierce G2 Fast Blotter

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Pierce™ Background Eliminator Kit Thermo Scientific™

The Thermo Scientific Pierce Background Eliminator Kit provides fast, easy removal of haze and artifacts on developed X-ray film to correct for overexposure of blots, whether from chemiluminescent Western blot or DNA or RNA autoradiography.

Features of the Background Eliminator Kit:

For overexposed X-ray film—reverses development of silver-based autoradiography film removing silver emulsion from the film surface
Recovers and preserved data—evenly reduces developed signal over the entire film so relative intensity (densitometry) of bands is not altered
Optimizes for signal-to-noise—allows readjustment of overdeveloped film to obtain the best image with respect to background haze, speckles and oversaturated bands that need to be resolved
Saves time and resources—eliminates the need to repeat experiments or re-expose a new piece of film to obtain an optimal image

This two-reagent kit is simple to use as demonstrated in the video below. Mix the two reagents (1:1 ratio) and immerse a developed piece of film (recent or old) in the working solution. Watch for 1 to 5 minutes until the desired image appears, then stop the reaction by rinsing the film in water. Pierce Background Eliminator for Film evenly reverses development-signal over the entire film, preserving relative densitometry values so that analysis and publication of results is still valid and possible.

Applications:
• X-ray film developed from enhanced chemiluminescence Western blots of any kind, whether from Thermo Scientific SuperSignal, Pierce ECL or other commercial substrate
• X-ray film developed from any chemiluminescent or radioactive gel-shift assays, including Thermo Scientific LightShift Chemiluminescent EMSA Kits for DNA or RNA
• Autoradiography film developed from chemiluminescent or isotopic (32P or 35S) Northern or Southern blots, including with Thermo Scientific North2South Chemiluminescent Detection Kit
• Any developed silver-emulsion photographic film, whether blue, clear, single- or double-sided, and regardless of the method of exposure

High background, shading, overexposed bands and speckling are problems inherent to film exposure. High background and shading are caused by overexposure, poor use of blocking buffer or inappropriate enzyme-labeled probe or antibody concentration. Overexposed bands are a common occurrence when the enzyme-labeled probe or antibody used is too concentrated or if the film was exposed for too long. Speckling and shading occur when enzyme conjugates form complexes and precipitate on the blot. Pierce Background Eliminator can correct all these problems without the need to re-expose a blot to film or re-do an entire experiment. The working solution can be used with newly exposed films or those that have been stored for years. In addition, the product can be used with any brand of film.

Nitrocellulose Membrane, 0.2 µm, 7.9 cm x 10.5 cm Thermo Scientific™

These Thermo Scientific Pierce Nitrocellulose Membranes are 0.2 µM, 7.9 cm x 10.5 cm sheets of nitrocellulose that have excellent binding properties for western blotting, dot-blot assays and other protein or nucleic acid methods.

Features of Nitrocellulose Membranes:

High-quality—pure, 100% nitrocellulose membranes with high surface area and excellent uniformity
Peptide applications—0.2 µM pore size
Convenient—7.9 cm x 10.5 cm pre-cut sheets
High-binding affinity—provides excellent protein binding, blocks easily, and produces very low background in chemiluminescent Western blotting

Nitrocellulose is a popular binding matrix for Western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (e.g., chemiluminescence, chromogenic, fluorescence).

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Nitrocellulose Membrane, 0.2 µm, 8 cm x 12 cm
Nitrocellulose Membrane, 0.2 µm, 8 cm x 8 cm

PVDF Transfer Membranes, 0.45 μm, 10 x 10 cm Thermo Scientific™

Pierce PVDF Transfer Membranes are made of high-quality polyvinylidene difluoride and provide high binding capacity for proteins and nucleic acids for western, southern, and northern blotting methods. The 0.45-µm pore size makes these membranes ideal for protein transfer for proteins >10 kDa.

See all membranes ›

Features
Membrane: polyvinylidene difluoride (PVDF)
Binding capacity: goat IgG: 294 µg/cm2; BSA: 215 µg/cm2; insulin: 160 µg/cm2
Re-probe characteristics: yes
Pre-activation: required with 100% alcohol (methanol, ethanol, or isopropanol)
• Compatibility*: compatible with commonly used transfer conditions and detection methods such as staining, chemiluminescence, and radiolabeling
Durability: compatible with most organic solvents, acids, and mild bases

*For fluorescent applications use low-fluorescent PVDF membranes.

Nitrocellulose Membranes, 0.45 μm, 8 x 12 cm Thermo Scientific™

Pierce Nitrocellulose Membranes, 0.45 µm, 8 x 12 cm, have excellent binding properties for western blotting, dot-blot assays,and other protein or nucleic acid methods. The 0.45-µm pore size is ideal for the transfer of most proteins (>20 kDa) and nucleic acids (>300 bp).

See all membranes ›

Features:
Membrane: 100% pure nitrocellulose
Binding capacity: ~80 µg/cm2 of proteins
Compatibility: compatible with commonly used transfer conditions and detection methods, such as staining, immunodetection, chemiluminescence, fluorescence, and radiolabeling

Pierce™ 1-Step Transfer Buffer Thermo Scientific™

Thermo Scientific Pierce 1-Step Transfer Buffer is designed for rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.

Features of 1-Step Transfer Buffer:

Fast—high ionic strength formulation provides 5- to 10-minute protein transfer when used with compatible fast semi-dry blotting systems
Ready to use—supplied as a 1X solution that is stable and ready to use at room temperature
Optimized—designed for seamless and reliable performance with the Pierce G2 Fast Blotter
Compatible—effective with other semi-dry transfer devices equipped with a suitable high-current power supply, including the original Pierce Fast Semi-Dry Blotter (Part No. 88217)
Economical—excellent performance without the special or costly consumables that are required by other fast semi-dry transfer devices

When used with the Pierce G2 Fast Blotter, this buffer provides effective gel-to-membrane transfer of proteins in 5 to 10 minutes with efficiency that is equal to, or better than, conventional Western blotting techniques. Pierce 1-Step Transfer Buffer is also compatible with other protein semi-dry transfer devices, including the original Pierce Fast Semi-Dry Blotter (Part No. 88217), when they are paired with a suitable high-current power supply. Such devices provide constant high current (1.3 to 5.0 amps) to rapidly transfer proteins via the high ionic strength conditions supplied by the transfer buffer.

Requires:
Pierce G2 Fast Blotter (Part No. 62288) or other semi-dry transfer device that is equipped or paired with a capable high-current power supply.

Fast blotting methods require a high-current power supply, such as the Pierce G2 Fast Blotter, and an optimized high ionic strength transfer buffer, such as Pierce 1-Step Transfer Buffer. By increasing the current, excellent transfer efficiency can be achieved in much shorter time compared to conventional methods. Amperage is held at a constant rate based on the surface area of the transfer stack(s) (~22-23mA/cm2) and voltage is limited to 25V.
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