ELISA substrates and reagents are designed to facilitate the detection and quantitation of biological samples in multiple immunoassay formats. Products include chemiluminescent, chemifluorescent, and colorimetric substrates.
Thermo Scientific Pierce 1-Step Ultra TMB ELISA Substrate (1 L) detects horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.
The ELISA Buffer Kit contains all the basic buffers necessary for use of Invitrogen Antibody Pairs or that are required to run an ELISA. The kit provides sufficient material for 10 ELISA plates. Kit components: • Coating Buffer A (10 mM phosphate buffer, pH 7.
Thermo Scientific Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.
Thermo Scientific Pierce 1-Step Turbo TMB-ELISA Substrate Solution rapidly detects horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.
Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. This product is a ready-to-use (1X) proprietary buffered solution of PNPP.
Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. This product comes in 5 mg tablets, with an inert filler as the balance.
This kit contains the key components required for the analysis of ten sandwich ELISA plates. It has been specifically formulated to be used in conjunction with PeproTech standard TMB ELISA development kits.
Thermo Scientific SuperSignal ELISA Femto Substrate is formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications. Features of SuperSignal ELISA Femto Substrate: • Immediate light generation—intense signal generated immediately both at room temperature and...
Thermo Scientific Pierce 1-Step Slow TMB ELISA Substrate Solution detects horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.
ELISA Blocker Blocking Buffer is a protein buffer that effectively blocks plate binding sites that remain in polystyrene microplate wells following initial coating steps in ELISA procedures. The ready-to-use buffer is optimized and routinely used for sandwich ELISA procedures involving cytokines,...
The ELISA-Light™ Immunoassay System with CSPD™ and Emerald-II™ Substrate⁄Enhancer Solution is used for ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.
Stop Solution is 0.16M sulfuric acid for use with the ELISA substrate 3,3’,5,5’ - tetramethylbenzidine (TMB). In the presence of horseradish peroxidase (HRP) enzyme conjugates, TMB and peroxide react to produce a blue byproduct having maximum absorbance at 605nm.
Thermo Scientific SuperSignal ELISA Pico Chemiluminescent Substrate is optimized to generate an intense light signal and provide exceptional performance in luminometer-based assays. Features of SuperSignal ELISA Pico Chemiluminescent Substrate: • Immediate light generation—intense signal is produced...
Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. Features of PNPP (p-nitrophenyl phosphate): • One-component alkaline phosphatase (AP) substrate • Absorbance maximum of 405nm • Develops a yellow hue in...
The ELISA/ELISPOT Coating Buffer Powder is designed and tested for use as the working solution for coating the ELISA or ELISPOT capture antibody to the ELISA or ELISPOT plate microwells. This has been tested with all cytokine ELISA and ELISPOT capture antibodies, according to the procedures...