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ELISA Substrates and Reagents
ELISA substrates and reagents are designed to facilitate the detection and quantitation of biological samples in multiple immunoassay formats. Products include chemiluminescent, chemifluorescent, and colorimetric substrates.
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TMB (3,3',5,5' tetramethylbenzidine) is a chromagen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) with major absorbances at 370 nm and 652 nm. The color then changes to yellow (measured at 450 nm) upon addition of sulfuric or phosphoric acid to stop the reaction.
For Use With (Application): ELISA. Form: Concentrate. Shipping Condition: Wet Ice. Quantity: 10 plates.
Form: Liquid. Shipping Condition: Ambient. Substrate Type: HRP, TMB. Product Type: ELISA Stop Solution.
Detection Method: Fluorescence. Product Line: QuantaRed™. Substrate Properties: Chemical Substrate. Substrate Type: HRP (Horseradish Peroxidase).
Detection Method: Chemiluminescence. Product Line: SuperSignal™. Substrate Properties: Chemical Substrate. Substrate Type: HRP (Horseradish Peroxidase).
Description: QuantaBlu Fluorogenic Peroxidase Substrate. Detection Method: Fluorescent. Product Line: QuantaBlu™. Substrate Properties: Chemical Substrate.
Detection Method: Colorimetric. Product Line: eBioscience™. Substrate Properties: Chemical Substrate. Substrate Type: HRP (Horseradish Peroxidase).
Thermo Scientific™ SuperSignal ELISA Femto Maximum Sensitivity Substrate is formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.
Suitable for use with all ELISA assays
For Use With (Application): ELISA. Form: Liquid. Product Type: Stop Solution. Quantity: 55 mL.
For Use With (Application): ELISA. For Use With (Application): ELISPOT. Form: Powder. Product Type: Coating Buffer.
Conjugate: Enzyme Labeled. For Use With (Application): ELISA. Form: Concentrate. Product Type: Coating Buffer.
Detection Method: Colorimetric. For Use With (Application): ELISA. Product Line: PeproTech. Substrate Type: ABTS, HRP.
Conjugate: Enzyme Labeled. For Use With (Application): ELISA. Form: Concentrate. Product Type: Coating Buffer.
Detection Method: Colorimetric. For Use With (Application): ELISA. Product Line: PeproTech. Substrate Type: TMB, HRP.
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Enzyme-linked immunoassays (ELISAs) are used to quantitate specific proteins in solution. The detection enzyme can be introduced into the assay in a number of ways ( Figure 1 ). Horseradish peroxidase (HRP) and alkaline phosphatase (AP) are the most commonly used enzyme labels, mainly due to the...
ELISA buffers and reagents are important components to develop excellent assay performance for high sensitivity, low background, and blocking non-specific binding. Select from a wide variety of easy-to-use and reliable reagents including blocking buffers, coating buffers, wash buffers, and...
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Can I dilute the SuperSignal ELISA Femto Maximum Sensitivity Substrate working reagent if my signal is too intense?
Can I dilute the SuperSignal ELISA Pico Chemiluminescent Substrate working reagent if my signal is too intense?