ELISA substrates and reagents are designed to facilitate the detection and quantitation of biological samples in multiple immunoassay formats. Products include chemiluminescent, chemifluorescent, and colorimetric substrates.
TMB (3,3',5,5' tetramethylbenzidine) is a chromagen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) with major absorbances at 370 nm and 652 nm. The color then changes to yellow (measured at 450 nm) upon addition of sulfuric or phosphoric acid to stop the reaction.
Thermo Scientific™ SuperSignal ELISA Femto Maximum Sensitivity Substrate is formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.
Enzyme-linked immunoassays (ELISAs) are used to quantitate specific proteins in solution. The detection enzyme can be introduced into the assay in a number of ways ( Figure 1 ). Horseradish peroxidase (HRP) and alkaline phosphatase (AP) are the most commonly used enzyme labels, mainly due to the...
ELISA buffers and reagents are important components to develop excellent assay performance for high sensitivity, low background, and blocking non-specific binding. Select from a wide variety of easy-to-use and reliable reagents including blocking buffers, coating buffers, wash buffers, and...