ELISA Substrates and Reagents

ELISA Substrates and Reagents

ELISA substrates and reagents are designed to facilitate the detection and quantitation of biological samples in multiple immunoassay formats. Products include chemiluminescent, chemifluorescent, and colorimetric substrates.
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1-15 of 51 Products

ELISA Stop Solution Invitrogen™

ELISA Stop Solution is a ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution. As TMB substrate solution reacts with horseradish peroxidase, it produces a pronounced blue color.

ELISA Buffer Kit Invitrogen™

The ELISA Buffer Kit contains all the basic buffers necessary for use of Invitrogen Antibody Pairs or that are required to run an ELISA. The kit provides sufficient material for 10 ELISA plates. Kit components: • Coating Buffer A (10 mM phosphate buffer, pH 7.

1-Step™ Ultra TMB-ELISA Substrate Solution Thermo Scientific™

TMB (3,3',5,5' tetramethylbenzidine) is a chromagen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) with major absorbances at 370 nm and 652 nm. The color then changes to yellow (measured at 450 nm) upon addition of sulfuric or phosphoric acid to stop the reaction.

SuperSignal™ ELISA Pico Chemiluminescent Substrate Thermo Scientific™

Thermo Scientific SuperSignal ELISA Pico Chemiluminescent Substrate is optimized to generate an intense light signal and provide exceptional performance in luminometer-based assays. Features of SuperSignal ELISA Pico Chemiluminescent Substrate: • Immediate light generation—intense signal is produced...

eBioscience™ TMB Solution (1X) Invitrogen™

The 1x eBioscience TMB Solution is a sensitive chromogenic substrate supplied as single ready-to-use solution. TMB (3,3’,5,5’-Tetramethylbenzidine) is a substrate for horseradish peroxidase (HRP) and is recommended for Ready-SET-Go! ELISA kits.

Stop Solution for TMB Substrates Thermo Scientific™

Stop Solution is 0.16M sulfuric acid for use with the ELISA substrate 3,3’,5,5’ - tetramethylbenzidine (TMB). In the presence of horseradish peroxidase (HRP) enzyme conjugates, TMB and peroxide react to produce a blue byproduct having maximum absorbance at 605nm.

Wash Buffer (25X)

Wash buffer can be used for all ELISA assays.

Super AquaBlue ELISA Substrate Invitrogen™

Our new Super AquaBlue ELISA Substrate is an enhanced ABTS (2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)) substrate solution for ELISA which provides high signal and sensitivity, low background. It is safe, stable, and extremely convenient to use.

ELISA Carbonate Coating Buffer Thermo Scientific™

ELISA Carbonate Coating Buffer is intended for use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA. The buffer is a 50 mM carbonate buffer, pH 9.4. For a complete kit of ELISA buffers, see ELISA Buffer Kit.

InstantOne ELISA Cell Lysis Buffer (5X) Invitrogen™

Cell Lysis Buffer (5X) is included in the InstantOne ELISA kits. The solution is offered individually as some lab protocols require addition buffers. Cell Lysis Buffer(5X) is used along with Enhancer Solution (Cat. No. IOES1) to make the Cell Lysis mixed used in the InstantOne phospho ELISA kits.

eBioscience™ ELISA/ELISPOT Diluent (5X) Invitrogen™

The 5X ELISA/ELISPOT Diluent is a Phosphate Buffered Solution supplemented with fetal bovine serum as a source of animal serum proteins. This buffer is used for blocking non-specific binding of ELISA plates, diluting recombinant standards and test samples, and making the working dilutions of the...

OPD (o-phenylenediamine dihydrochloride) Thermo Scientific™

Thermo Scientific Pierce OPD is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492nm by ELISA plate readers. OPD yields a water soluble yellow-orange product when reacted with peroxidase with an absorbance maximum of 492.

DynaLight™ Substrate with RapidGlow™ Enhancer Invitrogen™

Achieve faster ELISA results with DynaLight™ Substrate with RapidGlow™ Enhancer, a ready-to-use chemiluminescent substrate formulation that has been optimized to achieve faster results in solution-based assays.

CDP-Star™ Substrate (0.25 mM Ready-To-Use) Invitrogen™

The CDP-Star™ Substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.

Pierce™ Diethanolamine Substrate Buffer (5X) Thermo Scientific™

Thermo Scientific Pierce Diethanolamine Substrate Buffer (5X) include optimized, reliable and convenient stock solutions of diethanolamine for alkaline phosphatase (alk-phos) detection. Features of Diethanolamine Substrate Buffer (5X): • Ready-to-use • Minimize assay-to-assay variability...
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