ELISA Substrates and Reagents

ELISA Substrates and Reagents

ELISA substrates and reagents are designed to facilitate the detection and quantitation of biological samples in multiple immunoassay formats. Products include chemiluminescent, chemifluorescent, and colorimetric substrates.
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Sensitivity

Substrate Properties

Validated Application

eBioscience™ ELISA Wash Buffer, 1L packets Invitrogen™

The eBioscience ELISA Wash Buffer Powder is a dried powder formulation of phosphate buffered saline with 0.05% Tween-20. Each packet is reconstituted in 1 Liter of distilled, deionized water; the resulting 1x PBS/Tween-20 buffer (pH 7.4) is suitable for washing ELISA plate microwells.

eBioscience™ ELISA/ELISPOT Diluent (5X) Invitrogen™

The 5X ELISA/ELISPOT Diluent is a Phosphate Buffered Solution supplemented with fetal bovine serum as a source of animal serum proteins. This buffer is used for blocking non-specific binding of ELISA plates, diluting recombinant standards and test samples, and making the working dilutions of the...

InstantOne ELISA Cell Lysis Buffer (5X) Invitrogen™

Cell Lysis Buffer (5X) is included in the InstantOne ELISA kits. The solution is offered individually as some lab protocols require addition buffers. Cell Lysis Buffer(5X) is used along with Enhancer Solution (Cat. No. IOES1) to make the Cell Lysis mixed used in the InstantOne phospho ELISA kits.

ELISA-Light™ Immunoassay System with CSPD™ and Emerald-II™ Substrate/Enhancer Solution Applied Biosystems™

The ELISA-Light™ Immunoassay System with CSPD™ and Emerald-II™ Substrate⁄Enhancer Solution is used for ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.

ELISA-Light™ Immunoassay System with CSPD™ and Sapphire-II™ Substrate/Enhancer Solution Applied Biosystems™

The ELISA-Light™ Immunoassay System with CSPD™ and Sapphire-II™ Substrate⁄Enhancer Solution is used for ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.

Galacto-Light™ Reaction Buffer Diluent with Galacton-Plus™ Substrate Invitrogen™

The Galacto-Light™ Reaction Buffer Diluent and the Galacton-Plus™ chemiluminescent substrate solution are used with the Galacto-Light™ Plus Reporter Gene Assay System. Use With Light Emission Accelerator-II For Research Use Only. Not for use in diagnostics procedures.

CSPD™ Substrate (0.25 mM Ready-To-Use) with Nitro-Block™ Enhancer Invitrogen™

This CSPD™ chemiluminescent substrate with Nitro-Block™ Enhancer is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.

ELISA Phosphate Coating Buffer Thermo Scientific™

ELISA Phosphate Coating Buffer is intended for use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA. The buffer is a 10 mM phosphate buffer, pH 7.4. For a complete kit of ELISA buffers, see ELISA Buffer Kit.

SuperSignal™ ELISA Femto Substrate Thermo Scientific™

Thermo Scientific SuperSignal ELISA Femto Substrate is formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications. Features of SuperSignal ELISA Femto Substrate: • Immediate light generation—intense signal generated immediately both at room temperature and...

1-Step™ ABTS Substrate Solution Thermo Scientific™

ABTS Substrate is a water-soluble peroxidase substrate that yields a measurable green end product for use in ELISA methods. ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) is a water-soluble HRP substrate that yields a green end product upon reaction with peroxidase.

1-Step™ PNPP Substrate Solution Thermo Scientific™

Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. This product is a ready-to-use (1X) proprietary buffered solution of PNPP.

ELISA Buffer Kit Invitrogen™

The ELISA Buffer Kit contains all the basic buffers necessary for use of Invitrogen Antibody Pairs or that are required to run an ELISA. The kit provides sufficient material for 10 ELISA plates. Kit components: • Coating Buffer A (10 mM phosphate buffer, pH 7.

OPD Substrate Tablets (o-phenylenediamine dihydrochloride) Thermo Scientific™

Thermo Scientific Pierce OPD is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492nm by ELISA plate readers. OPD yields a water soluble yellow-orange product when reacted with peroxidase with an absorbance maximum of 492.

ONPG (o-nitrophenyl-β-D-galactopyranoside) Thermo Scientific™

Thermo Scientific Pierce ONPG is the preferred colorimetric substrate for ELISA applications involving beta-galactosidase (b-Gal) as the reporter enzyme. When using beta-Galactosidase as the label for proteins in ELISA studies, a wide variety of substrates are available, including:...

Pierce™ TMB Substrate Kit Thermo Scientific™

Thermo Scientific Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.

1-Step™ Slow TMB-ELISA Substrate Solution Thermo Scientific™

Thermo Scientific Pierce 1-Step Slow TMB ELISA Substrate Solution detects horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.

QuantaBlu™ NS/K Fluorogenic Substrate Kit Thermo Scientific™

The Thermo Scientific QuantaBlu NS/K Fluorogenic Substrate Kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays. QuantaBlu Fluorogenic Peroxidase Substrates allow rapid kinetic ELISA assays to be performed with greater...

QuantaBlu™ Fluorogenic Peroxidase Substrate Kit Thermo Scientific™

The Thermo Scientific QuantaBlu Fluorogenic Peroxidase Substrate Kit is a three-component kit containing proprietary fluorescent substrate, peroxide and stop solutions for stopped (endpoint) or non-stopped assays.

SuperSignal™ ELISA Pico Chemiluminescent Substrate Thermo Scientific™

Thermo Scientific SuperSignal ELISA Pico Chemiluminescent Substrate is optimized to generate an intense light signal and provide exceptional performance in luminometer-based assays. Features of SuperSignal ELISA Pico Chemiluminescent Substrate: • Immediate light generation—intense signal is produced...

DynaLight™ Substrate with RapidGlow™ Enhancer Invitrogen™

Achieve faster ELISA results with DynaLight™ Substrate with RapidGlow™ Enhancer, a ready-to-use chemiluminescent substrate formulation that has been optimized to achieve faster results in solution-based assays.

ProQuantum™ Immunoassays Accessory Kit Invitrogen™

The ProQuantum Immunoassay Accessory Kit provides the common consumable plastics to be used with any ProQuantum immunoassay product, conveniently bundled for first-time users who may be uncertain of what is needed or low-volume users who do not wish to buy these plastics in bulk.

Wash Buffer (25X)

Wash buffer can be used for all ELISA assays.

ELISA-Light™ Immunoassay System with CDP-Star™ and Emerald-II™ Substrate/Enhancer Solution Applied Biosystems™

The ELISA-Light™ Immunoassay System with CDP-Star™ and Emerald-II™ Substrate⁄Enhancer Solution is used for ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.

CDP-Star™ Substrate (0.25 mM Ready-To-Use) with Nitro-Block-II™ Enhancer Invitrogen™

The CDP-Star™ Substrate with Nitro-Block-II™ Enhancer is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This CDP-Star™ chemiluminescent substrate for alkaline phosphatase let you detect alkaline phosphatase and...

CDP-Star™ Substrate (0.4 mM Ready-To-Use) with Sapphire-II™ Enhancer, Size A Invitrogen™

The CDP-Star™ Substrate with Sapphire-II™ Enhancer is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.

cAMP-Screen™ Wash Buffer Invitrogen™

This Wash Buffer is suitable for use with the cAMP-Screen™ or cAMP-Screen Direct™ Immunoassay system. For Research Use Only. Not for use in diagnostics procedures.

CDP-Star™ Substrate (0.4 mM Ready-To-Use) with Emerald-II™ Enhancer, Size A Invitrogen™

The CDP-Star™ Substrate with Emerald-II™ Enhancer is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.

cAMP-Screen™ Assay/Lysis Buffer Invitrogen™

This Assay⁄Lysis Buffer is suitable for use with the cAMP-Screen™ or cAmp-Screen Direct™ Immunoassay System. For Research Use Only. Not for use in diagnostics procedures.

Assay Buffer (10X) Invitrogen™

This Assay Buffer is used in immunoassays and in Western and Southern blotting for alkaline phosphatase Tropix™ substrate conjugates. For Research Use Only. Not for use in diagnostics procedures.

CSPD™ Substrate (0.4 mM Ready-To-Use) with Emerald-II™ Enhancer, Size A Invitrogen™

This CSPD™ substrate with Emerald-II™ Enhancer is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.

Galacton-Star™ Substrate (50X Concentrate) Invitrogen™

Galacton-Star Substrate is a chemiluminescent substrate designed for the rapid, sensitive detection of β-galactosidase in cell lysates. The chemiluminescent assay exhibits over three orders of magnitude greater sensitivity compared to colorimetric β-galactosidase assays.

Super AquaBlue ELISA Substrate Invitrogen™

Our new Super AquaBlue ELISA Substrate is an enhanced ABTS (2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)) substrate solution for ELISA which provides high signal and sensitivity, low background. It is safe, stable, and extremely convenient to use.

eBioscience™ TMB Solution (1X) Invitrogen™

The 1x eBioscience TMB Solution is a sensitive chromogenic substrate supplied as single ready-to-use solution. TMB (3,3’,5,5’-Tetramethylbenzidine) is a substrate for horseradish peroxidase (HRP) and is recommended for Ready-SET-Go! ELISA kits.

ELISA/ELISPOT Coating Buffer Powder Invitrogen™

The ELISA/ELISPOT Coating Buffer Powder is designed and tested for use as the working solution for coating the ELISA or ELISPOT capture antibody to the ELISA or ELISPOT plate microwells. This has been tested with all cytokine ELISA and ELISPOT capture antibodies, according to the procedures...

ELISA Blocker Blocking Buffer Thermo Scientific™

ELISA Blocker Blocking Buffer is a protein buffer that effectively blocks plate binding sites that remain in polystyrene microplate wells following initial coating steps in ELISA procedures. The ready-to-use buffer is optimized and routinely used for sandwich ELISA procedures involving cytokines,...

ELISA Wash Buffer (30X) Thermo Scientific™

ELISA Wash Buffer is a Tris-based wash buffer for use in cytokine and other sandwich ELISA procedures. The buffer is the same formulation that is supplied with most Pierce ELISA kits for cytokines and chemokines.

Stop Solution for TMB Substrates Thermo Scientific™

Stop Solution is 0.16M sulfuric acid for use with the ELISA substrate 3,3’,5,5’ - tetramethylbenzidine (TMB). In the presence of horseradish peroxidase (HRP) enzyme conjugates, TMB and peroxide react to produce a blue byproduct having maximum absorbance at 605nm.

CSPD™ Substrate (0.25 mM Ready-To-Use) Invitrogen™

This CSPD™ substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.

Galacto-Star™ Reaction Buffer Diluent with Galacton-Star™ Substrate Invitrogen™

This is the Galacton-Star™ chemiluminescent substrate solution and substrate dilution buffer for the detection of E. coli β-galactosidase for use with the Galacto-Star™ One-Step β-Galactosidase Reporter Gene Assay System. For Research Use Only. Not for use in diagnostics procedures.

ELISA-Light™ Immunoassay System with CDP-Star™ and Sapphire-II™ Substrate/Enhancer Solution Applied Biosystems™

The ELISA-Light™ Immunoassay System with CDP-Star™ and Sapphire-II™ Substrate⁄Enhancer Solution is used for ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.

CSPD™ Substrate (0.4 mM Ready-To-Use) with Sapphire-II™ Enhancer, Size B Invitrogen™

This CSPD™ substrate with Sapphire-II™ Enhancer is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.

Pierce™ PNPP Substrate Kit Thermo Scientific™

Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. Our Phosphatase Substrate Kit contains PNPP tablets and a Diethanolamine Buffer to yield more than one liter of substrate.

ELISA Carbonate Coating Buffer Thermo Scientific™

ELISA Carbonate Coating Buffer is intended for use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA. The buffer is a 50 mM carbonate buffer, pH 9.4. For a complete kit of ELISA buffers, see ELISA Buffer Kit.

QuantaRed™ Enhanced Chemifluorescent HRP Substrate Kit Thermo Scientific™

The Thermo Scientific QuantaRed Enhanced Chemifluorescent HRP Substrate (ADHP) is the most sensitive fluorescent ELISA substrate, providing sensitivity comparable to enhanced chemiluminescence. This kit contains QuantaRed ADHP concentrate, QuantaRed Enhancer Solution, QuantaRed Stable Peroxide...

1-Step™ Ultra TMB-ELISA Substrate Solution Thermo Scientific™

Thermo Scientific Pierce 1-Step Ultra TMB ELISA Substrate (1 L) detects horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.

Pierce™ Diethanolamine Substrate Buffer (5X) Thermo Scientific™

Thermo Scientific Pierce Diethanolamine Substrate Buffer (5X) include optimized, reliable and convenient stock solutions of diethanolamine for alkaline phosphatase (alk-phos) detection. Features of Diethanolamine Substrate Buffer (5X): • Ready-to-use • Minimize assay-to-assay variability...

PNPP Substrate Thermo Scientific™

Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. Features of PNPP (p-nitrophenyl phosphate): • One-component alkaline phosphatase (AP) substrate • Absorbance maximum of 405nm • Develops a yellow hue in...

1-Step™ Turbo TMB-ELISA Substrate Solution Thermo Scientific™

Thermo Scientific Pierce 1-Step Turbo TMB-ELISA Substrate Solution rapidly detects horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.

OPD (o-phenylenediamine dihydrochloride) Thermo Scientific™

Thermo Scientific Pierce OPD is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492nm by ELISA plate readers. OPD yields a water soluble yellow-orange product when reacted with peroxidase with an absorbance maximum of 492.

PNPP Substrate Tablets Thermo Scientific™

Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. This product comes in 5 mg tablets, with an inert filler as the balance.

Pierce™ Stable Peroxide Substrate Buffer (10X) Thermo Scientific™

Thermo Scientific Pierce Stable Peroxide Substrate Buffer (10X) include optimized, reliable and convenient stock solutions of peroxide for HRP-based assays detection. Features of Stable Peroxide Substrate Buffer (10X): • Ready-to-use • Minimize assay-to-assay variability Buffered stable hydrogen...

CDP-Star™ Substrate (0.25 mM Ready-To-Use) Invitrogen™

The CDP-Star™ Substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.
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