ELISA Substrates and Reagents

ELISA Substrates and Reagents

ELISA substrates and reagents are designed to facilitate the detection and quantitation of biological samples in multiple immunoassay formats. Products include chemiluminescent, chemifluorescent, and colorimetric substrates.
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Sensitivity

Substrate Properties

Validated Application

1-Step™ ABTS Substrate Solution Thermo Scientific™

ABTS Substrate is a water-soluble peroxidase substrate that yields a measurable green end product for use in ELISA methods. ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) is a water-soluble HRP substrate that yields a green end product upon reaction with peroxidase.

1-Step™ PNPP Substrate Solution Thermo Scientific™

Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. This product is a ready-to-use (1X) proprietary buffered solution of PNPP.

ELISA Buffer Kit Invitrogen™

The ELISA Buffer Kit contains all the basic buffers necessary for use of Invitrogen Antibody Pairs or that are required to run an ELISA. The kit provides sufficient material for 10 ELISA plates. Kit components: • Coating Buffer A (10 mM phosphate buffer, pH 7.

OPD Substrate Tablets (o-phenylenediamine dihydrochloride) Thermo Scientific™

Thermo Scientific Pierce OPD is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492nm by ELISA plate readers. OPD yields a water soluble yellow-orange product when reacted with peroxidase with an absorbance maximum of 492.

ONPG (o-nitrophenyl-β-D-galactopyranoside) Thermo Scientific™

Thermo Scientific Pierce ONPG is the preferred colorimetric substrate for ELISA applications involving beta-galactosidase (b-Gal) as the reporter enzyme. When using beta-Galactosidase as the label for proteins in ELISA studies, a wide variety of substrates are available, including:...

Pierce™ TMB Substrate Kit Thermo Scientific™

Thermo Scientific Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.

1-Step™ Slow TMB-ELISA Substrate Solution Thermo Scientific™

Thermo Scientific Pierce 1-Step Slow TMB ELISA Substrate Solution detects horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.

QuantaBlu™ NS/K Fluorogenic Substrate Kit Thermo Scientific™

The Thermo Scientific QuantaBlu NS/K Fluorogenic Substrate Kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays. QuantaBlu Fluorogenic Peroxidase Substrates allow rapid kinetic ELISA assays to be performed with greater...

QuantaBlu™ Fluorogenic Peroxidase Substrate Kit Thermo Scientific™

The Thermo Scientific QuantaBlu Fluorogenic Peroxidase Substrate Kit is a three-component kit containing proprietary fluorescent substrate, peroxide and stop solutions for stopped (endpoint) or non-stopped assays.

SuperSignal™ ELISA Pico Chemiluminescent Substrate Thermo Scientific™

Thermo Scientific SuperSignal ELISA Pico Chemiluminescent Substrate is optimized to generate an intense light signal and provide exceptional performance in luminometer-based assays. Features of SuperSignal ELISA Pico Chemiluminescent Substrate: • Immediate light generation—intense signal is produced...

DynaLight™ Substrate with RapidGlow™ Enhancer Invitrogen™

Achieve faster ELISA results with DynaLight™ Substrate with RapidGlow™ Enhancer, a ready-to-use chemiluminescent substrate formulation that has been optimized to achieve faster results in solution-based assays.

ProQuantum™ Immunoassays Accessory Kit Invitrogen™

The ProQuantum Immunoassay Accessory Kit provides the common consumable plastics to be used with any ProQuantum immunoassay product, conveniently bundled for first-time users who may be uncertain of what is needed or low-volume users who do not wish to buy these plastics in bulk.

Wash Buffer (25X)

Wash buffer can be used for all ELISA assays.

ELISA-Light™ Immunoassay System with CDP-Star™ and Emerald-II™ Substrate/Enhancer Solution Applied Biosystems™

The ELISA-Light™ Immunoassay System with CDP-Star™ and Emerald-II™ Substrate⁄Enhancer Solution is used for ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.

CDP-Star™ Substrate (0.25 mM Ready-To-Use) with Nitro-Block-II™ Enhancer Invitrogen™

The CDP-Star™ Substrate with Nitro-Block-II™ Enhancer is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This CDP-Star™ chemiluminescent substrate for alkaline phosphatase let you detect alkaline phosphatase and...
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