ELISA Substrates and Reagents

ELISA Substrates and Reagents

ELISA substrates and reagents are designed to facilitate the detection and quantitation of biological samples in multiple immunoassay formats. Products include chemiluminescent, chemifluorescent, and colorimetric substrates.
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Substrate Properties

Validated Application

Super AquaBlue ELISA Substrate Invitrogen™

Our new Super AquaBlue ELISA Substrate is an enhanced ABTS (2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)) substrate solution for ELISA which provides high signal and sensitivity, low background. It is safe, stable, and extremely convenient to use.

eBioscience™ TMB Solution (1X) Invitrogen™

The 1x eBioscience TMB Solution is a sensitive chromogenic substrate supplied as single ready-to-use solution. TMB (3,3’,5,5’-Tetramethylbenzidine) is a substrate for horseradish peroxidase (HRP) and is recommended for Ready-SET-Go! ELISA kits.

ELISA/ELISPOT Coating Buffer Powder Invitrogen™

The ELISA/ELISPOT Coating Buffer Powder is designed and tested for use as the working solution for coating the ELISA or ELISPOT capture antibody to the ELISA or ELISPOT plate microwells. This has been tested with all cytokine ELISA and ELISPOT capture antibodies, according to the procedures...

ELISA Blocker Blocking Buffer Thermo Scientific™

ELISA Blocker Blocking Buffer is a protein buffer that effectively blocks plate binding sites that remain in polystyrene microplate wells following initial coating steps in ELISA procedures. The ready-to-use buffer is optimized and routinely used for sandwich ELISA procedures involving cytokines,...

ELISA Wash Buffer (30X) Thermo Scientific™

ELISA Wash Buffer is a Tris-based wash buffer for use in cytokine and other sandwich ELISA procedures. The buffer is the same formulation that is supplied with most Pierce ELISA kits for cytokines and chemokines.

Stop Solution for TMB Substrates Thermo Scientific™

Stop Solution is 0.16M sulfuric acid for use with the ELISA substrate 3,3’,5,5’ - tetramethylbenzidine (TMB). In the presence of horseradish peroxidase (HRP) enzyme conjugates, TMB and peroxide react to produce a blue byproduct having maximum absorbance at 605nm.

CSPD™ Substrate (0.25 mM Ready-To-Use) Invitrogen™

This CSPD™ substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.

Galacto-Star™ Reaction Buffer Diluent with Galacton-Star™ Substrate Invitrogen™

This is the Galacton-Star™ chemiluminescent substrate solution and substrate dilution buffer for the detection of E. coli β-galactosidase for use with the Galacto-Star™ One-Step β-Galactosidase Reporter Gene Assay System. For Research Use Only. Not for use in diagnostics procedures.

ELISA-Light™ Immunoassay System with CDP-Star™ and Sapphire-II™ Substrate/Enhancer Solution Applied Biosystems™

The ELISA-Light™ Immunoassay System with CDP-Star™ and Sapphire-II™ Substrate⁄Enhancer Solution is used for ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.

CSPD™ Substrate (0.4 mM Ready-To-Use) with Sapphire-II™ Enhancer, Size B Invitrogen™

This CSPD™ substrate with Sapphire-II™ Enhancer is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.

Pierce™ PNPP Substrate Kit Thermo Scientific™

Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. Our Phosphatase Substrate Kit contains PNPP tablets and a Diethanolamine Buffer to yield more than one liter of substrate.

ELISA Carbonate Coating Buffer Thermo Scientific™

ELISA Carbonate Coating Buffer is intended for use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA. The buffer is a 50 mM carbonate buffer, pH 9.4. For a complete kit of ELISA buffers, see ELISA Buffer Kit.

QuantaRed™ Enhanced Chemifluorescent HRP Substrate Kit Thermo Scientific™

The Thermo Scientific QuantaRed Enhanced Chemifluorescent HRP Substrate (ADHP) is the most sensitive fluorescent ELISA substrate, providing sensitivity comparable to enhanced chemiluminescence. This kit contains QuantaRed ADHP concentrate, QuantaRed Enhancer Solution, QuantaRed Stable Peroxide...

1-Step™ Ultra TMB-ELISA Substrate Solution Thermo Scientific™

Thermo Scientific Pierce 1-Step Ultra TMB ELISA Substrate (1 L) detects horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.

Pierce™ Diethanolamine Substrate Buffer (5X) Thermo Scientific™

Thermo Scientific Pierce Diethanolamine Substrate Buffer (5X) include optimized, reliable and convenient stock solutions of diethanolamine for alkaline phosphatase (alk-phos) detection. Features of Diethanolamine Substrate Buffer (5X): • Ready-to-use • Minimize assay-to-assay variability...
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