ELISA Substrates and Reagents

ELISA substrates and reagents are designed to facilitate the detection and quantitation of biological samples in multiple immunoassay formats. Products include chemiluminescent, chemifluorescent, and colorimetric substrates.
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Sensitivity

Substrate Properties

Validated Application

ELISA Phosphate Coating Buffer Thermo Scientific™

ELISA Phosphate Coating Buffer is intended for use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA. The buffer is a 10 mM phosphate buffer, pH 7.4. For a complete kit of ELISA buffers, see ELISA Buffer Kit.

eBioscience™ ELISA Wash Buffer, 1L packets Invitrogen™

The eBioscience ELISA Wash Buffer Powder is a dried powder formulation of phosphate buffered saline with 0.05% Tween-20. Each packet is reconstituted in 1 Liter of distilled, deionized water; the resulting 1x PBS/Tween-20 buffer (pH 7.4) is suitable for washing ELISA plate microwells.

eBioscience™ ELISA/ELISPOT Diluent (5X) Invitrogen™

The 5X ELISA/ELISPOT Diluent is a Phosphate Buffered Solution supplemented with fetal bovine serum as a source of animal serum proteins. This buffer is used for blocking non-specific binding of ELISA plates, diluting recombinant standards and test samples, and making the working dilutions of the...

1-Step™ ABTS Substrate Solution Thermo Scientific™

ABTS Substrate is a water-soluble peroxidase substrate that yields a measurable green end product for use in ELISA methods. ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) is a water-soluble HRP substrate that yields a green end product upon reaction with peroxidase.

1-Step™ PNPP Substrate Solution Thermo Scientific™

Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. This product is a ready-to-use (1X) proprietary buffered solution of PNPP.

ONPG (o-nitrophenyl-β-D-galactopyranoside) Thermo Scientific™

Thermo Scientific Pierce ONPG is the preferred colorimetric substrate for ELISA applications involving beta-galactosidase (b-Gal) as the reporter enzyme. When using beta-Galactosidase as the label for proteins in ELISA studies, a wide variety of substrates are available, including:...

OPD Substrate Tablets (o-phenylenediamine dihydrochloride) Thermo Scientific™

Thermo Scientific Pierce OPD is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492nm by ELISA plate readers. OPD yields a water soluble yellow-orange product when reacted with peroxidase with an absorbance maximum of 492.

SuperSignal™ ELISA Pico Chemiluminescent Substrate Thermo Scientific™

Thermo Scientific SuperSignal ELISA Pico Chemiluminescent Substrate is optimized to generate an intense light signal and provide exceptional performance in luminometer-based assays. Features of SuperSignal ELISA Pico Chemiluminescent Substrate: • Immediate light generation—intense signal is produced...

SuperSignal™ ELISA Femto Substrate Thermo Scientific™

Thermo Scientific SuperSignal ELISA Femto Substrate is formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications. Features of SuperSignal ELISA Femto Substrate: • Immediate light generation—intense signal generated immediately both at room temperature and...

1-Step™ Slow TMB-ELISA Substrate Solution Thermo Scientific™

Thermo Scientific Pierce 1-Step Slow TMB ELISA Substrate Solution detects horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.

Pierce™ TMB Substrate Kit Thermo Scientific™

Thermo Scientific Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.

QuantaBlu™ Fluorogenic Peroxidase Substrate Kit Thermo Scientific™

The Thermo Scientific QuantaBlu Fluorogenic Peroxidase Substrate Kit is a three-component kit containing proprietary fluorescent substrate, peroxide and stop solutions for stopped (endpoint) or non-stopped assays.

QuantaBlu™ NS/K Fluorogenic Substrate Kit Thermo Scientific™

The Thermo Scientific QuantaBlu NS/K Fluorogenic Substrate Kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays. QuantaBlu Fluorogenic Peroxidase Substrates allow rapid kinetic ELISA assays to be performed with greater...

DynaLight™ Substrate with RapidGlow™ Enhancer Invitrogen™

Achieve faster ELISA results with DynaLight™ Substrate with RapidGlow™ Enhancer, a ready-to-use chemiluminescent substrate formulation that has been optimized to achieve faster results in solution-based assays.

ProQuantum™ Immunoassays Accessory Kit Invitrogen™

The ProQuantum Immunoassay Accessory Kit provides the common consumable plastics to be used with any ProQuantum immunoassay product, conveniently bundled for first-time users who may be uncertain of what is needed or low-volume users who do not wish to buy these plastics in bulk.
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