ELISA Substrates and Reagents

ELISA Substrates and Reagents

ELISA substrates and reagents are designed to facilitate the detection and quantitation of biological samples in multiple immunoassay formats. Products include chemiluminescent, chemifluorescent, and colorimetric substrates.
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Validated Application


Substrate Properties

ELISA Carbonate Coating Buffer Thermo Scientific™

ELISA Carbonate Coating Buffer is intended for use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA. The buffer is a 50 mM carbonate buffer, pH 9.4. For a complete kit of ELISA buffers, see ELISA Buffer Kit.

Galacto-Star™ Reaction Buffer Diluent with Galacton-Star™ Substrate Invitrogen™

This is the Galacton-Star® chemiluminescent substrate solution and substrate dilution buffer for the detection of E. coli β-galactosidase for use with the Galacto-Star™ One-Step β-Galactosidase Reporter Gene Assay System. For Research Use Only. Not for use in diagnostics procedures.

CSPD™ Substrate (0.25 mM Ready-To-Use) Invitrogen™

This CSPD® substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.

CDP-Star™ Substrate (0.25 mM Ready-To-Use) Invitrogen™

The CDP-Star® Substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.

eBioscience™ TMB Solution (1X) Invitrogen™

The 1x eBioscience TMB Solution is a sensitive chromogenic substrate supplied as single ready-to-use solution. TMB (3,3’,5,5’-Tetramethylbenzidine) is a substrate for horseradish peroxidase (HRP) and is recommended for Ready-SET-Go! ELISA kits.

ELISA/ELISPOT Coating Buffer Powder Invitrogen™

The ELISA/ELISPOT Coating Buffer Powder is designed and tested for use as the working solution for coating the ELISA or ELISPOT capture antibody to the ELISA or ELISPOT plate microwells. This has been tested with all cytokine ELISA and ELISPOT capture antibodies, according to the procedures...

Super AquaBlue ELISA Substrate Invitrogen™

Our new Super AquaBlue ELISA Substrate is an enhanced ABTS (2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)) substrate solution for ELISA which provides high signal and sensitivity, low background. It is safe, stable, and extremely convenient to use.

QuantaRed™ Enhanced Chemifluorescent HRP Substrate Kit Thermo Scientific™

The Thermo Scientific QuantaRed Enhanced Chemifluorescent HRP Substrate (ADHP) is the most sensitive fluorescent ELISA substrate, providing sensitivity comparable to enhanced chemiluminescence. This kit contains QuantaRed ADHP concentrate, QuantaRed Enhancer Solution, QuantaRed Stable Peroxide...

1-Step™ Turbo TMB-ELISA Substrate Solution Thermo Scientific™

Thermo Scientific Pierce 1-Step Turbo TMB-ELISA Substrate Solution rapidly detects horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.

PNPP Substrate Thermo Scientific™

Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. Features of PNPP (p-nitrophenyl phosphate): • One-component alkaline phosphatase (AP) substrate • Absorbance maximum of 405nm • Develops a yellow hue in...

Pierce™ PNPP Substrate Kit Thermo Scientific™

Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. Our Phosphatase Substrate Kit contains PNPP tablets and a Diethanolamine Buffer to yield more than one liter of substrate.

Pierce™ Stable Peroxide Substrate Buffer (10X) Thermo Scientific™

Thermo Scientific Pierce Stable Peroxide Substrate Buffer (10X) include optimized, reliable and convenient stock solutions of peroxide for HRP-based assays detection. Features of Stable Peroxide Substrate Buffer (10X): • Ready-to-use • Minimize assay-to-assay variability Buffered stable hydrogen...

OPD (o-phenylenediamine dihydrochloride) Thermo Scientific™

Thermo Scientific Pierce OPD is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492nm by ELISA plate readers. OPD yields a water soluble yellow-orange product when reacted with peroxidase with an absorbance maximum of 492.

PNPP Substrate Tablets Thermo Scientific™

Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. This product comes in 5 mg tablets, with an inert filler as the balance.

1-Step™ Ultra TMB-ELISA Substrate Solution Thermo Scientific™

Thermo Scientific Pierce 1-Step Ultra TMB ELISA Substrate (1 L) detects horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.
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