Shop All Electrophoresis Gel Stains

GelCode™ Blue Safe Protein Stain Thermo Scientific™

GelCode Blue Safe Protein Stain is a fast and sensitive coomassie-dye reagent that provides one-step ultrasensitive detection of proteins in polyacrylamide gels and is formulated to avoid hazardous shipping.

Sensitivity—detect down to 9 ng of protein per band in 15 minutes
Safety—non-corrosive to skin, non-flammable, and safe to ship and store until activated
Additional reagents—water washes only; no acid-fixative or methanol destain solutions required
Compatibility—suitable for staining 1D and 2D polyacrylamide gels and nitrocellulose and PVDF membranes (multiple protocols provided in the instructions)
Applications—qualitative visualization, quantitative densitometry, and downstream excision and analysis by mass spectrometry
Easy to use—add and dissolve activator crystals before first use, then simply add reagent to stain gels
Stable—store non-activated or activated stain at room temperature for up to one year

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Because the supplied activator crystals are packaged and shipped dry in a separate bottle, GelCode Blue Safe Protein Stain is classified as nonhazardous under the U.S. Department of Transportation (DOT) shipping requirements. For proper protein staining, the activator crystals must be dissolved in the staining reagent before use. Add the entire contents of the activator crystals bottle into the GelCode Blue Safe Protein Stain bottle. Dissolve the crystals by mixing end-over-end for one minute, and then allow it to remain at room temperature for five minutes before use. Store the activated stain at room temperature.

SYBR™ Green II RNA Gel Stain, 10,000X concentrate in DMSO Invitrogen™

SYBR Green II RNA gel stain is a sensitive nucleic acid gel stain that has bright fluorescence when bound to RNA and low background in gels, making it ideal for use with either formaldehyde/agarose or polyacrylamide gels using laser scanners or standard UV transilluminators. A 500 µL unit size (S-7564) and 1mL unit size (S-7568) are available.

Pro-Q™ Diamond Phosphoprotein Gel Staining Kit (includes 200 mL Stain, 40 µL Standard) Invitrogen™

The Pro-Q® Diamond Phosphoprotein Gel Staining Kit, includes 200 mL of the Pro-Q® Diamond Phosphoprotein Gel Stain and 40 µL of the PeppermintStick™ phosphoprotein molecular weight standards. The Pro-Q® Diamond phosphoprotein gel stain is a fluorescent stain (excitation/emission maxima ~555/580 nm) that can detect as little as 1-16 ng of phosphoprotein per band with a signal that is linear over 3 orders of magnitude. The PeppermintSTick™ phosphoprotein molecular weight standards are a mixture of phosphorylated and nonphosphorylated proteins with molecular weights from 14,000 to 116,250 daltons that serve both as molecular weight markers and as positive and negative controls.

WesternDot™ 625 Goat Anti-Rabbit Western Blot Kit Invitrogen™

WesternDot® 625 Western Blot kits utilize extremely bright Qdot® 625 nanocrystals and include reagents for sensitive imaging of proteins probed with mouse or rabbit primary antibodies. With detection performance and ease-of-use that exceed existing ECL-based methods, the WesternDot® 625 kits make use of our extremely bright Qdot 625 streptavidin conjugate to allow direct imaging of blots while avoiding the need to spend time and money on optimizing multiple exposures to film in the dark. Each kit contains reagents sufficient for 20 minigel blots (7 cm × 10 cm/blot)

Pierce™ Color Silver Stain Kit Thermo Scientific™

Thermo Scientific Pierce Color Silver Stain Kit detects proteins electrophoresed in 1D and 2D polyacrylamide gels using metallic silver to produce color variations that facilitate differentiation of protein bands and spots.

Features of the Color Silver Stain Kit:

Customizable—kit is a reagent set that allows customized formulation of working reagents and staining protocols for particular gel formats and silver-staining applications
Sensitive—detects many proteins at 0.1ng per square millimeter in standard-thickness gels
Broad protein detectability—stains a wider range of protein types than monochromatic silver stains (proteins that do not bind silver are revealed as yellow bands or spots)
Color differentiation—gel-separated proteins and polypeptide-containing macromolecules stain in five basic colors: black, blue, brown, red and yellow
Profiling resolution—multicolored detection of DNA, lipids and polysaccharides in addition to typical proteins provides additional landmarks to aid in 2D gel profiling

The Color Silver Stain Kit contains four reagents and a flexible protocol for staining proteins in 1D and 2D electrophoresis gels. The method requires more time than monochromatic silver stains but provides greater flexibility in adapting the concentration of working solutions to obtain effective staining with different gel types and thicknesses and unusual protein targets. Proteins stain as black, blue, brown, red or yellow bands or spots according to a combination of their specific charge and other characteristics. Once results are known for a particular protein of interest, these slight color differences provide for easy band and spot identification.

• Four reagent solutions (500 mL each): silver, reducer base, reducer aldehyde and stabilizer base

The Color Silver Stain Kit enables highly sensitive and versatile staining of proteins electrophoresed in 1D and 2D polyacrylamide gels. Generally, bands containing less than 1ng of protein are detectable with this silver stain. Proteins stain in reproducibly distinct colors visible against an amber background. Nucleic acids, as in the products of PCR, that have been electrophoresed in polyacrylamide gels also may be stained using this kit.

Several slightly different silver staining methods were developed in the early 1980s. All methods depend on the kinetics of impregnation, reduction of silver ions to elemental silver and its complexation to protein nucleation sites in a porous gel matrix. The Color Silver Stain Kit uses a weakly acidic solution of silver nitrate for gel impregnation, followed by development in formaldehyde at alkaline pH. After incubation in the Stabilizer Base Working Solution, silver nitrate and formaldehyde are either reacted or diffused from the gel.

When a stained gel is viewed over a bright white light source, several distinct colors may be recognized. Five basic colors commonly visible in protein samples with the Color Silver Stain are black, blue, brown, red and yellow. Color differences correspond to differences in protein amino acid composition, although detailed predictions are not possible. Nevertheless, color patterns are reproducible for a given sample and staining time and temperature, and they may be used to distinguish overlapping proteins and other subtleties in 2D systems.

Regardless of gel size and thickness, the Color Silver Stain Kit is well characterized and supported by this complete procedure for obtaining excellent staining results for routine and special electrophoresis experiments involving polyacrylamide gels.

PageBlue™ Protein Staining Solution Thermo Scientific™

PageBlue Protein Staining Solution is a sensitive, economical formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid.

Easy-to-use—simply soak the gel in the ready-to-use solution and observe stained bands without destaining
Staining time—25 to 40 minute protocol
Safe—does not contain methanol or acetic acid
Dynamic range—linear dynamic range of 5 to 500 ng
Economical—can be reused up to three times

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The simple PageBlue Protein Staining Solution protocol is sensitive, efficient, and eliminates the concern of over-staining gels, even after overnight staining. This protein stain delivers a dynamic range of 5 to 500 ng, which is approximately ten times more sensitive than traditional coomassie R-250-based dyes.

Pro-Q™ Emerald 300 Glycoprotein Gel Stain Kit, with SYPRO™ Ruby Protein Gel Stain Invitrogen™

Molecular Probes' proprietary Pro-Q Emerald 300 and Pro-Q Emerald 488 Glycoprotein Stain Kits provide the most advanced technology available for detection of glycoproteins in gels and on blots. Gel staining is rapid and very sensitive. In less than three hours, it is possible to detect as little as 300 pg of glycoprotein per band, depending on the degree of glycosylation, making these stains at least 50-fold more sensitive than standard fuchsin staining.

Pierce™ Silver Stain Rescue Reagent Thermo Scientific™

The Thermo Scientific Pierce Silver Stain Rescue Reagent enables removal of undesirable background from unevenly and overly developed silver-stained polyacrylamide electrophoresis gels.

Features of the Pierce Silver Stain Rescue Reagent:

Easy-to-use—mix the reagents at the recommended dilution and it is ready to rescue silver-stained gels that have dark backgrounds as a result of over-development
Simple and effective—eliminates background signal in minutes, allowing speedy but controlled reversal of the staining process to meet your objective; complete the entire process in approx. 1 hour
Saves time and money—procedure is much simpler, many times faster, and significantly less costly than repeating gel-electrophoresis and staining
Preserves valuable data—uniformly removes silver from the entire polyacrylamide gel, enabling improvement of band visibility without altering or manipulating the data
Compatible with any silver stain—removes high background or non-uniform staining, regardless of the silver stain formulation, whether commercial or homemade

This simple, two-reagent kit recovers and corrects overdeveloped silver-stained gels by uniformly removing developed silver. By slowly reversing the development process over several minutes, the Pierce Silver Stain Rescue Reagent allows one to monitor and stop destaining when the level is best for the bands of interest. In most silver staining procedures for protein gels, addition of stop-solution must be carefully timed to achieve optimal staining results; frequently, otherwise useful SDS-PAGE data are obscured by over-development at this critical step. The Silver Stain Rescue Reagent can recover over-stained gels, eliminating the need to repeat the experiment (run and stain a new gel). Most excessively stained mini gels can be corrected in less than one hour, depending on the working concentration selected.

Pierce Silver Stain Rescue Reagent enables removal of undesirable background from unevenly and overly developed silver-stained gels. Anomalies in the gel matrix, prolonged development or overloaded protein often cause high background and uneven staining in silver-stained gels. Such staining problems often impede detection of important results and prevent use of the gel image for publications. The Silver Stain Rescue Reagent enables simple recovery of a quality stained gel image by effectively reversing the silver development process. The rescue reagent working solution is easily prepared, and the procedure is performed at room temperature in an ordinary lighted laboratory. The formulation removes the silver stain from gels slowly so that the destaining process can be easily monitored and stopped when the desired level of background has been removed. Additionally, a slight modification of the default working solution formulation enables flexibility in destaining time and image quality.

SilverQuest™ Silver Staining Kit Invitrogen™

The SilverQuest Silver Staining Kit provides a rapid and easy method to silver stain proteins and DNA in polyacrylamide gels. It is specifically designed to provide sensitive silver staining compatible with mass spectrometry analysis.

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Features of this kit:
Compatible—staining compatible with mass spectrometry analysis
Protocol—protein bands (0.3 ng of protein) can be visualized within an hour or for added speed a microwave procedure can be completed in ~30 minutes
Color-coded solutions—color-coded solutions for easy staining protocol

Mass spectrometry-compatible reagents
The SilverQuest Silver Staining Kit has been specifically designed to make protein detection compatible with mass spectrometry analysis. The SilverQuest reagent set contains a sensitizer that contains no glutaraldehyde or formaldehyde, so it leaves lysine and arginine side-chains unmodified. Additionally, the SilverQuest destaining solution effectively removes silver from protein bands in polyacrylamide gels. Peptides remain as efficient targets for in-gel trypsin digestion and gel extraction and yield better coverage during mass spectrometry analysis.

CandyCane™ Glycoprotein Molecular Weight Standards Invitrogen™

CandyCane glycoprotein molecular weight standards separate into alternating bands of glycosylated and nonglycosylated proteins during polyacrylamide gel electrophoresis.

Krypton™ Fluorescent Protein Stain Thermo Scientific™

Thermo Scientific Krypton Fluorescent Protein Stain enables sensitive visualization with fluorescence imagers (520nm excitation; 580nm emission) of proteins separated in polyacrylamide gels or transferred to membranes.

Features of Krypton Fluorescent Protein Stain:

Intense fluorescent stain—excitation and emission maxima of 520nm and 580nm, respectively (compatible with 532nm excitation light sources and 580 to 600nm emission filters)
Instrument-compatible—effective with popular laser-based and filter-based fluorescence imagers
Broad dynamic range—linear quantitative range spans three to four orders of magnitude Sensitive—detect as little as 0.25ng protein with the standard protocol (approx. 2.5 hours); at least as sensitive as fluorescent stains from other suppliers
Fast—standard (2.5 hours) and rapid (30 minutes) protocols are significantly faster than procedures of other popular fluorescent stains
Versatile—compatible with proteomics workflows involving downstream mass spectrometry
Compact—supplied as a 10X solution to save storage space; just dilute with water before use

This fluorescent protein gel stain is for bright yellow-orange fluorescent detection of proteins separated by 1D or 2D polyacrylamide gel electrophoresis (SDS-PAGE). Krypton Stain uses a protein-binding dye with fluorescence properties (Ex/Em = 520/580nm) suitable for visualization with a variety of standard laser-based and filter-based fluorescence imagers and documentation systems. Proteins are detectable at concentrations greater than 0.25ng per band in a typical mini-gel lane (greater than 2ng for the rapid staining protocol). Krypton Stain provides high signal intensity, exhibits minimal protein-to-protein variation, has an excellent quantitative dynamic range and is compatible with subsequent destaining and analysis by mass spectrometry.

For best results, detect bands using visible laser-based imagers equipped with a 532nm laser light source. Although 580nm-filters are optimal for emission, 600nm-filters are also compatible. Stained gels can be imaged on any platform with the respective excitation and emission filters.

SYBR™ Safe DNA Gel Stain in 0.5X TBE Invitrogen™

SYBR Safe DNA gel stain was developed specifically for reduced mutagenicity – to be safer than ethidium bromide for staining DNA in agarose or acrylamide gels. SYBR Safe stain is not only less mutagenic than ethidium bromide, but SYBR Safe stain's detection sensitivity is better than that of ethidium bromide. SYBR Safe stain comes as a premixed solution that can be used just like an ethidium bromide solution, either in the gel during the electrophoresis run or as a post-run stain. Bound to nucleic acids, the green-fluorescent SYBR Safe stain has fluorescence excitation maxima at ~280 and ~502 nm, and an emission maximum at ~530 nm. SYBR Safe DNA gel stain is also available as 1 L (S-33100) of ready-to-use solution. The SYBR Safe DNA Gel Stain Starter Kit (S-33110) includes 1 L of SYBR Safe DNA gel stain and one photographic filter (S-37100).

Coomassie Fluor™ Orange Protein Gel Stain (Ready-To-Use Solution) Invitrogen™

Molecular Probes' proprietary Coomassie Fluor Orange protein gel stain provides fast, simple, sensitive staining of proteins separated by SDS-PAGE. After electrophoresis, the gel is stained, rinsed and photographed. Staining is complete in less than an hour – no separate fixation or destaining steps are required, and there is no risk of overstaining the gel. Stained proteins can be visualized using a standard 300 nm UV transilluminator or a laser-based scanner. Coomassie Fluor Orange protein gel stain is also available in a 5 liter size (Cat. no. C-33251).

InVision™ His-Tag In-Gel Staining Kit Invitrogen™

The InVision His-tag In-gel Staining Kit includes InVision His-tag In-gel Stain as well as BenchMark His-tagged Protein Standard. InVision His-tag In-gel Stain is a ready-to-use, fast, sensitive, and highly specific stain for visualizing His-tagged fusion protein bands directly in a polyacrylamide gel following electrophoresis.

• Stain proteins fused to an oligohistidine sequence
• Eliminate western blotting—detect His-tagged proteins directly in the gel
• Detect nanogram levels of protein bands with a UV transilluminator or imaging equipment with appropriate filters or lasers
• Visualize results in under three hours

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Fast and easy staining
The InVision His-tag In-gel Stain protocol is easy to perform and requires very little hands-on-time, allowing for rapid and scalable protein expression screening in a variety of gel types. Following electrophoresis, the gel is fixed, incubated in the stain, washed, and the specific His-tagged fusion protein detected by exposing the gel to UV or visible light. In under three hours, nanogram levels of His-tagged fusion protein can be detected. After documenting your InVision Stain signal, total protein staining can be performed.

PeppermintStick™ Phosphoprotein Molecular Weight Standards Invitrogen™

PeppermintStick Phosphoprotein Molecular Weight Standards are a mixture of phosphorylated and nonphosphorylated proteins ranging from 14.4 to 116 kDa. Separation by polyacrylamide gel electrophoresis resolves the standard mixture into two phosphorylated and four nonphosphorylated protein bands.

• Accurate protein sizing on SDS-polyacrylamide gels and western blots.
• Positive and negative controls for detection of phosphorylated proteins, such as the methods provided by our Pro-Q® Diamond phosphoprotein gel stain

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