Shop All Electrophoresis Gel Stains

SYBR™ Safe DNA Gel Stain in 0.5X TBE Invitrogen™

SYBR Safe DNA gel stain was developed specifically for reduced mutagenicity – to be safer than ethidium bromide for staining DNA in agarose or acrylamide gels. SYBR Safe stain is not only less mutagenic than ethidium bromide, but SYBR Safe stain's detection sensitivity is better than that of ethidium bromide. SYBR Safe stain comes as a premixed solution that can be used just like an ethidium bromide solution, either in the gel during the electrophoresis run or as a post-run stain. Bound to nucleic acids, the green-fluorescent SYBR Safe stain has fluorescence excitation maxima at ~280 and ~502 nm, and an emission maximum at ~530 nm. SYBR Safe DNA gel stain is also available as 1 L (S-33100) of ready-to-use solution. The SYBR Safe DNA Gel Stain Starter Kit (S-33110) includes 1 L of SYBR Safe DNA gel stain and one photographic filter (S-37100).

SYBR™ Safe DNA Gel Stain Invitrogen™

SYBR® Safe DNA Gel Stain is a highly sensitive stain for visualization of DNA in agarose or acrylamide gels. SYBR® Safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide that can utilize either blue light or UV excitation.

• Reduce your exposure to highly mutagenic ethidium bromide and harmful UV light
• Increase your sensitivity by reducing nonspecific background fluorescence
• Use in place of ethidium bromide for all staining applications, including RNA staining

A better DNA stain
SYBR® Safe DNA Gel Stain is a better nucleic acid staining reagent for all of your molecular biology needs. Not only is SYBR® Safe DNA Gel Stain better for you and the environment, it’s better for your sample and your institution. Read more as to why SYBR® Safe DNA gel stain is your better option.

Safer than ethidium bromide
SYBR® Safe DNA Gel Stain has been specifically formulated and evaluated in a battery of toxicity tests with results indicating so that it is less mutagenic and safer for you to work with than ethidium bromide. An independent laboratory showed reduced mutagenicity of SYBR® Safe stain compared to ethidium bromide using the Ames test (see figure). You can further decrease your exposure risk by using visible blue-light illumination with SYBR® Safe stain rather than UV illumination. This is especially valuable when performing exposure-intensive protocols like cutting bands out of gels.

Excellent sensitivity
SYBR® Safe stain offers excellent sensitivity in nucleic acid visualization and documentation applications with either UV excitation or blue-light excitation. When bound to nucleic acids, the green-fluorescent SYBR® Safe stain has fluorescence excitation maxima at ~280 and ~502 nm, and an emission maximum at ~530 nm (see figure). Plus, when used with blue light illumination, SYBR® Safe stain has less background fluorescence than ethidium bromide–stained gels illuminated with UV light.

Easy to use
SYBR® Safe stain is supplied as a 10,000X concentrate in DMSO which can be used just like a solution of ethidium bromide. SYBR® Safe stain can be mixed into an agarose gel for staining during electrophoresis or the gel can be incubated in a solution of SYBR® Safe stain following electrophoresis. SYBR® Safe stain can be stored at room temperature in its original packaging to avoid excessive light exposure. We also offer SYBR® Safe E-Gel pre-cast agarose gels for the ultimate in convenience.

SYBR™ Safe DNA Gel Stain Starter Kit Invitrogen™

SYBR Safe DNA gel stain was developed specifically for reduced mutagenicity – to be safer than ethidium bromide for staining DNA in agarose or acrylamide gels. SYBR Safe stain is not only less mutagenic than ethidium bromide, but SYBR Safe stain's detection sensitivity is better than that of ethidium bromide. SYBR Safe stain comes as a premixed solution that can be used just like an ethidium bromide solution, either in the gel during the electrophoresis run or as a post-run stain. Bound to nucleic acids, the green-fluorescent SYBR Safe stain has fluorescence excitation maxima at ~280 and ~502 nm, and an emission maximum at ~530 nm. SYBR Safe DNA gel stain is also available as 1 L (S-33100) or 4 L (S-33101) of ready-to-use solution.

SYBR™ Safe DNA Gel Stain in 1X TAE Invitrogen™

SYBR Safe™ DNA gel stain was developed specifically for reduced mutagenicity—to be safer than ethidium bromide for staining DNA in agarose or acrylamide gels. SYBR Safe™stain is not only less mutagenic than ethidium bromide, but SYBR Safe™ stain's detection sensitivity is better than that of ethidium bromide. SYBR Safe™ stain comes as a premixed solution that can be used just like an ethidium bromide solution, either in the gel during the electrophoresis run or as a post-run stain. Bound to nucleic acids, the green-fluorescent SYBR Safe™ stain has fluorescence excitation maxima at ~280 and ~502 nm, and an emission maximum at ~530 nm.

Coomassie Brilliant Blue R-250 Dye Thermo Scientific™

Thermo Scientific Pierce Coomassie Brilliant Blue R-250 is one of the most common forms of coomassie dye, which is a key component of various colorimetric protein gel stains.

Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and Bradford-type assay reagents for protein quantitation. The R-250 (red-tinted) form lacks two methyl groups that are present in the G-250 (green-tinted) form, which is also called colloidal coomassie dye. Typically, coomassie gel stains and protein assay reagents are formulated as very acidic solutions in 25 to 50% methanol. In acidic conditions, the dye binds to proteins primarily through basic amino acids (primarily arginine, lysine and histidine), and the number of coomassie dye ligands bound to each protein molecule is approximately proportional to the number of positive charges found on the protein. Protein-binding causes the dye to change from reddish-brown to bright blue (absorption maximum equals 595 nm).

Features of Coomassie Brilliant Blue R-250 and G-250 Dyes:

Easy detection—Develops intensely colored complexes with proteins
High sensitivity—Can determine as little as 0.5 µg/cm2 of protein present in a gel matrix
Reversible staining—Anion of Coomassie Brilliant Blue formed in the acidic staining medium combines with the protonated amino groups of proteins by electrostatic interaction; resulting complex is reversible under the proper conditions
Differentiation between bound and unbound dye—When dissolved in 0.01M citrate buffer at pH 3.0, has an absorption maximum at 555nm; protein-dye complex is characterized by a peak slightly broader than that of the free dye with a maximum at 549 nm

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Coomassie Brilliant Blue G-250 Dye

Coomassie Brilliant Blue G-250 Dye Thermo Scientific™

Thermo Scientific Pierce Coomassie Brilliant Blue G-250 is one of the most common forms of coomassie dye, which is a key component of various colorimetric protein gel stains.

Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and Bradford-type assay reagents for protein quantitation. The R-250 (red-tinted) form lacks two methyl groups that are present in the G-250 (green-tinted) form, which is also called colloidal coomassie dye. Typically, coomassie gel stains and protein assay reagents are formulated as very acidic solutions in 25 to 50% methanol. In acidic conditions, the dye binds to proteins primarily through basic amino acids (primarily arginine, lysine and histidine), and the number of coomassie dye ligands bound to each protein molecule is approximately proportional to the number of positive charges found on the protein. Protein-binding causes the dye to change from reddish-brown to bright blue (absorption maximum equals 595 nm).

Features of Coomassie Brilliant Blue R-250 and G-250 Dyes:

Easy detection—Develops intensely colored complexes with proteins
High sensitivity—Can determine as little as 0.5 µg/cm2 of protein present in a gel matrix
Reversible staining—Anion of Coomassie Brilliant Blue formed in the acidic staining medium combines with the protonated amino groups of proteins by electrostatic interaction; resulting complex is reversible under the proper conditions
Differentiation between bound and unbound dye—When dissolved in 0.01M citrate buffer at pH 3.0, has an absorption maximum at 555nm; protein-dye complex is characterized by a peak slightly broader than that of the free dye with a maximum at 549 nm

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Coomassie Brilliant Blue R-250 Dye

Electrophoretic Mobility-Shift Assay (EMSA) Kit, with SYBR™ Green & SYPRO™ Ruby EMSA stains Invitrogen™

This fluorescence-based Electrophoretic Mobility Shift Assay (EMSA) Kit provides a fast, easy and quantitative method to detect both nucleic acid and protein in the same gel. This kit uses two fluorescent dyes for detection - SYBR® Green EMSA nucleic acid gel stain for RNA or DNA detection and SYPRO® Ruby EMSA protein gel stain for protein detection. The nucleic acids and proteins are stained sequentially in the gel after electrophoresis, so there is no possibility that labeling will interfere with the protein binding being assayed. Staining only takes about 20 minutes for nucleic acid, and then about 4 hours for the subsequent protein staining.

SYPRO™ Red Protein Gel Stain (5000X in DMSO) Invitrogen™

SYPRO Red Protein Gel Stain is a sensitive, ready-to-use fluorescent stain for proteins in 1D gels. It offers many advantages over Coomassie Blue and silver staining, including a fast, one-step staining protocol requiring no destaining; a linear dynamic range over three orders of magnitude; and very little protein-to-protein variation in staining. Stained proteins can be viewed with a standard UV or blue-light transilluminator or imaging equipment containing the appropriate filers or laser.

Compare all fluorescent stains ›

Features:
Protocol—fast, one-step staining protocol requiring no destaining
• Linear quantitation range over three orders of magnitude
• Very little protein-to-protein variation in staining

Imperial™ Protein Stain Thermo Scientific™

Imperial Protein Stain is a ready-to-use colorimetric stain formulated with coomassie dye R-250 that delivers consistent nanogram-level detection of proteins in polyacrylamide electrophoresis gels or nitrocellulose membranes.

Features:
Sensitive—detects less than 3 ng protein per band with the enhanced protocol (3 hours)
Time to stain—ready-to-use reagent detects less than 6 ng protein per band in just 20 minutes
High contrast—intense purple bands are easier to photograph or scan than typical coomassie blue stains
Compatibility—compatible with downstream mass spectrometry analysis and protein sequencing
Additional reagents—water washes only; no acid-fixative or methanol destaining solutions required
Stable—1-year room-temperature stability

Compare all Coomassie stains ›

The stain is a unique formulation of coomassie brilliant blue R-250 that delivers substantial improvements in protein-staining performance compared to homemade or other commercial stains. Staining results in intensely colored protein bands that are easy to photograph and document with gel imagers. This reagent is one of the most sensitive colorimetric stains available, easily detecting 3 to 6 nanograms of protein per band. The easy-to-follow protocol is flexible to meet demanding time and sensitivity requirements.

Multiplexed Proteomics™ Phosphoprotein Gel Staining Kit (Includes MPP33301, 40 µl Standard and S12001) Invitrogen™

The Multiplexed Proteomics® Phosphoprotein Gel Stain Kit contains the Pro-Q® Diamond phosphoprotein gel stain and the SYPRO® Ruby protein gel stain. Determining the ratio of the Pro-Q® Diamond dye to the SYPRO® Ruby dye signal intensities for each band or spot provides a measure of the phosphorylation level normalized to the total amount of protein. Using both stains in combination makes it possible to distinguish a low amount of a highly phosphorlyated protein from a higher amount of a less phosphorylated protein. Both the SYPRO® Ruby (S12000, S12001, S21900) and Pro-Q® Diamond gel stains (MPP33300, MPP33301, MPP33302) are available separately.

Pierce™ Silver Stain Kit Thermo Scientific™

The Pierce Silver Stain Kit is a rapid, ultra-sensitive, and versatile silver stain system for protein detection in polyacrylamide gels, yielding consistent and reliable results. It is a metallic silver (Ag) protein stain that yields a remarkably clear and uniform gel background. In standard mini gels, proteins are detectable at greater than 0.25 ng per band or spot. The protocol has been optimized for flexibility by allowing short or overnight gel fixation and staining steps without affecting staining performance (sensitivity or clarity). A short (one-minute) sensitization step, performed after gel fixation, yields results that are free of the characteristically dark or blotchy backgrounds often seen with homemade or other commercially available silver stains. This feature is beneficial when performing densitometric analysis of silver stained gels.

Compare all silver stains ›

Features:
Sensitivity—detect proteins at 0.25 ng
Protocol—gel staining can be performed within 5 min to 20 hrs, depending on desired sensitivity
Gel-compatibility—staining performance is very good with a variety of commercial precast mini-gels and SDS-PAGE buffer-systems
Workflow-compatible—mild chemical formulation ensures compatibility with mass spectrometry, sequencing, and other downstream procedures that depend on destaining and protein recovery

Silver staining methods generally use either glutaraldehyde or formaldehyde, which cause some covalent crosslinking of protein to each other and the gel matrix. To the extent that this crosslinking occurs, extraction or elution of protein from the gel will be inhibited. Pierce Silver Stain uses formaldehyde in the stain and developer working solutions, but the concentration is low enough that irreversible protein crosslinking is minimized.

Pro-Q™ Diamond Phosphoprotein Gel Destaining Solution Invitrogen™

Pro-Q Diamond phosphoprotein gel stain provides a convenient method for selectively staining phosphoproteins in acrylamide gels, without the need for blotting or phosphoprotein specific antibodies and Western blot analysis. Preparation of the Pro-Q Diamond phosphoprotein gel destaining solution is described in the gel staining protocol, but we also provide the detaining solution premixed for your convenience.

Ethidium Bromide Solution (0.625 mg/mL) Thermo Scientific™

Thermo Scientific Pierce Ethidium Bromide is available as a dilute (0.625 mg/mL) solution to accommodate most molecular biology applications for fluorescent DNA visualization.

Features of Ethidium Bromide Solution:

• Each drop contains 25 µg of ethidium bromide and is ideal for a 50 mL agarose gel
• After adding one drop of solution per 50 mL of gel, the final concentration of EtBr will be 0.5 µg/mL, which is the recommended concentration for electrophoresis of nucleic acids
• Can also be used as the running buffer or for staining gels after electrophoresis

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Ethidium Bromide Solution (10 mg/mL)

Colloidal Blue Staining Kit Invitrogen™

The Colloidal Blue Staining Kit allows detection of nanogram levels of proteins in 1D or 2D PAGE gels with minimal effort and requires only water to destain. The Colloidal Blue stain uses colloidal chemistry that reduces free dye in solution and improves the protein-to-dye binding ratio. Samples are intensely stained and visible within three hours. Background staining is virtually eliminated by destaining overnight with water. The kit requires one easy solution preparation. Methanol is required in the staining step, but is not included in the kit.

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Pro-Q™ Emerald 300 Glycoprotein Gel and Blot Stain Kit Invitrogen™

Pro-Q Emerald 300 Glycoprotein Stain provides direct detection of glycoproteins in gels and on blots. Gel staining is rapid and very sensitive. Pro-Q Emerald glycoprotein stain reacts with periodate-oxidized carbohydrate groups, creating a bright green-fluorescent signal on glycoproteins. The staining procedure requires only three steps: fixation, oxidation, and staining—no reduction step is required. Depending on the nature and degree of glycosylation, the Pro-Q Emerald 300 stain allows the detection of as little as 1 ng of a glycoprotein per band in gels, making this stain about 50 times more sensitive than the standard periodic acid–Schiff base method using acidic fuchsin dye. Signal can be visualized using a 300 nm UV transilluminator.

Compare all glycoprotein stains ›

This stain can be combined with general protein stains (for example SYPRO Ruby) for dichromatic detection of glycoproteins and total proteins in gel and on blots.
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