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Genomics and Proteomics


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iBright™ CL750 Imaging System Invitrogen™

Capture western blot and gel images and analyze data efficiently and easily with the Invitrogen iBright CL750 Imaging System. This system supports basic imaging applications associated with chemiluminescent western blots, as well as fluorescent stained nucleic acid gels, fluorescent stained protein gels, and colorimetric stained protein gels.

For expanded functionality, we also offer the more powerful iBright CL1500 Imaging System and iBright FL1500 Imaging System. The iBright CL1500 Imaging System supports the main imaging applications associated with chemiluminescent and colorimetric western blots, as well as fluorescent stained nucleic acid gels, fluorescent stained protein gels, colorimetric stained protein gels, and colorimetric membrane stains. The iBright FL1500 Imaging System features the same imaging capabilities as the iBright CL1500 Imaging System, but also offers fluorescent western blot imaging capability.

Compare the iBright Imaging Systems ›

Features of the iBright CL750 Imaging System:
9.1 MP cooled CCD camera: high sensitivity and dynamic range to help enable the detection of subtle differences in samples
Smart Exposure technology: provides rapid determination of optimal exposure time to help minimize the need to repeat exposures to acquire the desired signal
Simple interface: clear layout of functions and features combined with a 12.1-inch capacitive touchscreen for a smooth imaging experience
22.5 x 18.0-cm field of view: large field of view for high-throughput imaging (image up to four mini or two midi blots at a time)
Green LED-based transilluminator: effectively excite popular DNA dyes such as ethidium bromide and SYBR Green dyes with an alternative to UV-based transilluminators
Flexible connectivity: export captured images via ethernet connection, Wi-Fi (with optional accessory), USB, or directly to our Connect cloud-based platform
Multiple image analysis options: perform densitometry, quantitation, and normalization directly using the on-instrument software, or for more in-depth analysis use iBright Analysis Software (available in both desktop and cloud-based versions)

Novex™ TBE Gels, 4-12%, 12 well Invitrogen™

Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments are clearly resolved into sharp, tight bands. Novex® TBE gels are designed to run on the XCell SureLock® Mini-Cell.

Formulation
Novex® TBE gels are manufactured with high-purity Tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS.

Recommended buffers
For optimum performance, Novex® TBE Running Buffer and Novex® Hi-Density TBE Sample Buffer are strongly recommended for use with these gels. Novex® Hi-Density TBE Sample Buffer contains the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, as well as the tracking dyes Bromophenol Blue and Xylene Cyanol.

Novex™ TBE Gels, 6%, 1 well Invitrogen™

Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments are clearly resolved into sharp, tight bands. Novex® TBE gels are designed to run on the XCell SureLock® Mini-Cell.

Formulation
Novex® TBE gels are manufactured with high-purity Tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS.

Recommended buffers
For optimum performance, Novex® TBE Running Buffer and Novex® Hi-Density TBE Sample Buffer are strongly recommended for use with these gels. Novex® Hi-Density TBE Sample Buffer contains the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, as well as the tracking dyes Bromophenol Blue and Xylene Cyanol.

A2 and A5 Large Gel Electrophoresis System Standard Comb Thermo Scientific™

Eliminate comb assembly with an assortment of heavy-duty, one-piece Fisherbrand™ A2 and A5 Large Gel Electrophoresis Systems Combs, for use with Owl A2 and A5 Large Gel Electrophoresis Systems.

Novex™ TBE Gels, 6%, 12 well Invitrogen™

Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments are clearly resolved into sharp, tight bands. Novex® TBE gels are designed to run on the XCell SureLock® Mini-Cell.

Formulation
Novex® TBE gels are manufactured with high-purity Tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS.

Recommended buffers
For optimum performance, Novex® TBE Running Buffer and Novex® Hi-Density TBE Sample Buffer are strongly recommended for use with these gels. Novex® Hi-Density TBE Sample Buffer contains the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, as well as the tracking dyes Bromophenol Blue and Xylene Cyanol.

E-Gel™ 1 Kb Plus DNA Ladder Invitrogen™

Invitrogen E-Gel 1 Kb Plus DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 15,000 bp. E-Gel 1 Kb Plus DNA Ladder consists of 18 individual chromatography-purified DNA fragments and has a reference band at 1,500 bp for easy orientation.

E-Gel 1 Kb Plus DNA Ladder is specifically formulated for optimal performance on pre-cast E-Gel agarose gels.

Highlights of E-Gel 1 Kb Plus DNA Ladder:
Performance—optimal for use on E-Gel agarose gels
Sharp, clear bands—chromatography purified fragments for consistent and reliable results and stable at room temperature up to six months
Ready-to-use—premixed with loading buffer
Convenient—provided with 1X E-Gel Sample Loading Buffer
Precise—an exact amount of DNA in each band

Product use
The double-stranded DNA ladder can be visualized on 0.8–1% E-Gel agarose gels. The ladder is designed with a uniform intensity of DNA bands for a clear view of each band. An exact amount of DNA in each band allows approximate quantification of DNA samples.

GeneRuler Express DNA Ladder Thermo Scientific™

Thermo Scientific GeneRuler Express DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 100 bp to 5,000 bp on agarose gels. The DNA ladder consists of 9 DNA fragments and is provided with 6X TriTrack DNA Loading Dye.

Highlights of Express DNA Ladder
Easy visualization—three-color tracking of DNA migration during electrophoresis with 6X TriTrack DNA Loading Dye (contains Xylene Cyanol FF, Bromophenol Blue, and Orange G)
Simplicity—one loading dye for a variety of samples
Sharp bands—clear results with mixtures of individual chromatography-purified DNA fragments

Product use
This double-stranded DNA ladder is compatible with 1% standard and precast agarose slab gels and can be visualized after ethidium bromide or SYBR Safe staining. A uniform intensity of bands and bright reference bands allow easy sizing and orientation. A defined amount of DNA in each band enables approximate quantification of sample DNA.

NuPAGE™ 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well Invitrogen™

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE gels for optimal results during day-to-day use.

Features of NuPAGE Bis-Tris gels:
• Better protein integrity—optimized sample preparation process preserves your proteins
• Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Learn more about all of our NuPAGE Bis-Tris gels >
View migration charts ›

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

We also provide NuPAGE Tris-Acetate gels for separating larger proteins. For classic Laemmli-based Tris-glycine electrophoresis, we provide Novex Tris-Glycine gels.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer. Rapid semi-dry transfer can be done using the Pierce Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the XCell II Blot Module or the Mini Blot Module.

Related links
Overview of 1D Protein Electrophoresis
Comparison of NuPAGE Tris-Bis vs. traditional Tris-glycine gels

Owl™ P10DS Dual Gel System Replacement Parts Thermo Scientific™

Keep your dual gel system running with Thermo Scientific™ Owl™ P10DS Dual Gel System Replacement Parts. Parts are for use with the Owl™ P10DS Dual Gel System, an optimal device for a large number of samples that need extended separation and mobility shift assays.

iBright™ Imaging System White Transilluminator Screen Invitrogen™

This is a replacement white transilluminator screen for the iBright imaging systems (CL1000, CL1500, FL1000, and FL1500). The white transilluminator screen provides a contrasting background when imaging protein gels stained with colorimetric stains such as coomassie and silver stains. This transilluminator screen is automatically detected by the iBright imaging system and is required to provide the desired image black/white orientation and band identification with colorimetric stained gels.

NorthernMax™ High Stringency Wash Buffer Invitrogen™

The equivalent of 0.1X SSC or SSPE, this Ambion® wash buffer is functionally tested by northern analysis and is certified RNase-free. Supplied in one bottle containing 1 L. NorthernMax® reagents are the same components included in the NorthernMax® Ultrasensitive Northern Blotting Kits but in larger sizes. These reagents are supplied ready to use and are certified RNase-free. Comprehensive instructions are included.

Novex™ Tricine Welcome Pack, 10-20%, 15-well Invitrogen™

The Novex Tricine Welcome Pack provides all of the necessary Novex Tricine gels, buffers, and reagents you will need to begin using the Mini Gel Tank.

The Novex Tricine Welcome Pack contains:
Mini Gel Tank
• Novex Tricine Mini Gels (2 boxes, 20 gels)
Novex Tricine SDS Running Buffer (10X)
Novex Tricine SDS Sample Buffer (2X)
NuPAGE Sample Reducing Agent (10X)
Spectra Multicolor Low Range Protein Ladder

About the Mini Gel Tank
The Mini Gel Tank is compatible with all Invitrogen Novex, NuPAGE, and Bolt mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies being used.

About Novex Tricine gels
Novex Tricine gels provide separation of low molecular weight proteins and peptides. The Tricine system is a modification of the tris-glycine discontinuous buffer system developed by Schaegger and von Jagow specifically for peptides and low molecular weight proteins. In this system the tricine replaces the glycine in the running buffer, resulting in more efficient stacking and de-stacking of low molecular weight proteins and higher resolution of smaller peptides.

Transfer
For transfer of proteins to a membrane, we recommend using Novex Tris-Glycine Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be performed using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Pierce™ Reversible Protein Stain Kit for PVDF Membranes Thermo Scientific™

The Thermo Scientific Pierce Reversible Protein Stain Kit for PVDF Membranes is a rapid and sensitive alternative to Ponceau S stain for protein detection on PVDF membrane after transfer from polyacrylamide gels.

Features of the Reversible Protein Stain Kit for PVDF Membranes:

Better than Ponceau S—more sensitive, easier to document, permanent until reversed (does not fade)
Sensitive—high-avidity, total-protein stain; lower limit of detection equals 25 to 50 ng per band
Specific—detects only protein; does not bind or interact with other electrophoresis or sample components
Rapid—stain in less than 30 minutes; completely erase and reverse staining in less than 15 minutes
Stable—components are stable at room temperature, saving refrigerator space and eliminating equilibration steps

This kit for membrane staining uses a nondestructive, reversible, reliable and sensitive method to stain and detect proteins on PVDF membranes. The kit is a superior alternative to Ponceau S stains for evaluating the efficiency of protein transfer following SDS-PAGE and before immunoblotting. The lower limit of detection with this method is 25 to 50 ng per band (at least five times more sensitive than traditional Ponceau S staining). The staining protocol is simple, quick and results in turquoise-blue bands that do not fade and are easily photographed for future reference. The stain can be easily reversed in less than 15 minutes. Subsequent western blot detection is unaffected because the stain does not alter the protein and is completely removed.

The Pierce Reversible Protein Stain Kit for PVDF Membranes has excellent avidity for a broad range of proteins, resulting in low protein-to-protein variability and enabling most proteins to be detected at very low levels (25 to 50ng per band). Pierce Membrane Stain is superior to other protein stains available for PVDF membranes. For example, Ponceau S is less sensitive and results in red bands that easily fade and are difficult to photograph. Coomassie dye is a sensitive stain, but it permanently binds to proteins and can interfere with western blotting.

The kit uses a dye that has a high affinity for protein but does not permanently bind. The stain has minimal nonspecific interactions with the membrane surface and protein-transfer reagents, and the technique is compatible with general western blot systems. The treated membrane does not interfere with conventional chemiluminescent or chromogenic detection using HRP and alkaline phosphatase substrates. In addition, the stain is compatible with N-terminal sequence analysis of proteins excised and eluted from membrane.

Related Products
Pierce™ Reversible Protein Stain Kit for Nitrocellulose Membranes
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Novex™ TBE Gels, 6%, 15 well Invitrogen™

Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments are clearly resolved into sharp, tight bands. Novex® TBE gels are designed to run on the XCell SureLock® Mini-Cell.

Formulation
Novex® TBE gels are manufactured with high-purity Tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS.

Recommended buffers
For optimum performance, Novex® TBE Running Buffer and Novex® Hi-Density TBE Sample Buffer are strongly recommended for use with these gels. Novex® Hi-Density TBE Sample Buffer contains the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, as well as the tracking dyes Bromophenol Blue and Xylene Cyanol.

E-Gel™ 1 Kb Plus Express DNA Ladder Invitrogen™

Invitrogen E-Gel 1 Kb Plus Express DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 5,000 bp. E-Gel 1 Kb Plus Express DNA Ladder consists of nine individual chromatography-purified DNA fragments with reference bands at 1,500 and 500 bp for easy orientation.

E-Gel 1 Kb Plus Express DNA Ladder is specifically formulated for optimal performance on pre-cast E-Gel agarose gels.

Highlights of E-Gel 1 Kb Plus Express DNA Ladder:
Performance—optimized for use on E-Gel CloneWell ll agarose gels
Sharp, clear bands—chromatography-purified fragments for consistent and reliable results, stable at room temperature up to six months
Ready to use—premixed with loading buffer
Convenient—provided with 1X E-Gel Sample Loading Buffer
Precise—an exact amount of DNA in each band

Product use
The double-stranded DNA ladder can be visualized on 0.8% E-Gel CloneWell II and E-Gel EX agarose gels. The ladder is designed with a uniform intensity of DNA bands for a clear view of each band. An exact amount of DNA in each band allows approximate quantification of DNA samples.

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