Shop All Gel Electrophoresis Equipment and Supplies

Novex Tris-Glycine Plus Midi Protein gels are improved polyacrylamide gels based on traditional Laemmli protein electrophoresis technology, allowing the use of Laemmli sample and running buffers. The gels offer reproducible separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine Plus Midi gels are a 1.0-mm thick, wider format (8 cm x 13 cm) gel for higher throughput electrophoresis of protein samples.

Learn more about our Novex Tris-Glcyine gels ›

View migration charts ›

Features of Novex Tris-Glycine Plus gels include:
Improved shelf life—store gels for up to 12 months at 4°C
Flexibility—use for native and denaturing protein assays
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine Plus Midi gels come in fixed concentrations of 10% and 12%, as well as gradient concentrations of 4–12%, 4–20%, and 8–16%. Select from our many well formats, including 12+2-, 20-, and 26-well.

Run your proteins in native or denatured form
Novex Tris-Glycine Plus gels do not contain SDS and so can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer. The gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion Cell using our adapters.

For transfer of proteins to a membrane, we recommend using the Novex Tris-Glycine Transfer Buffer when performing traditional wet transfer. Rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE gels for optimal results during day-to-day use.

Features of NuPAGE Bis-Tris gels:
• Better protein integrity—optimized sample preparation process preserves your proteins
• Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Learn more about all of our NuPAGE Bis-Tris gels >
View migration charts ›

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

We also provide NuPAGE Tris-Acetate gels for separating larger proteins. For classic Laemmli-based Tris-glycine electrophoresis, we provide Novex Tris-Glycine gels.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer. Rapid semi-dry transfer can be done using the Pierce Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the XCell II Blot Module or the Mini Blot Module.

Related links
Overview of 1D Protein Electrophoresis
Comparison of NuPAGE Tris-Bis vs. traditional Tris-glycine gels

Invitrogen NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. They are high performance polyacrylamide gels that simulate the denaturing or native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of high molecular weight proteins compared to traditional Tris-glycine SDS-PAGE gels.

Features of NuPAGE Tris-Acetate gels:
• High resolution—gels offer optimal separation of high molecular weight proteins
• Better protein integrity—sample preparation process has been optimized to help preserve proteins
• Longer self life—gels can be stored for at least eight months

Learn more about our NuPAGE Tris-Acetate gels ›
View migration charts ›

Choose the right NuPAGE Tris-Acetate gel for your protein separation
Obtain optimal separation of your high molecular weight proteins by choosing the right combination of gel and running buffer. NuPAGE Tris-Acetate protein gels come in a polyacrylamide concentration of 7% and a 3–8% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Tris-Acetate gels also come in multiple well formats. Mini gels can be run using our XCell SureLock Mini-Cell or Mini Gel Tank. Midi gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion™ Cell using our adapters.

Run your proteins in native or denatured form
NuPAGE Tris-Acetate protein gels do not contain SDS and can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using NuPAGE LDS Sample Buffer and NuPAGE Tris-Acetate SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the XCell II Blot Module or the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

Thermo Scientific GeneRuler 100 bp Plus DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 100 bp to 3,000 bp on agarose or polyacrylamide gels. The DNA ladder consists of 14 DNA fragments and is provided with 6X TriTrack DNA Loading Dye.

Highlights of 100 bp Plus DNA Ladder
• Easy visualization—three-color tracking of DNA migration during electrophoresis with 6X TriTrack DNA Loading Dye (contains Xylene Cyanol FF, Bromophenol Blue, and Orange G)
• Simplicity—one loading dye for a variety of samples
• Sharp bands—clear results with mixtures of individual chromatography-purified DNA fragments

Product use
This double-stranded DNA ladder is compatible with 1–2% standard and precast agarose slab gels and can be visualized after ethidium bromide or SYBR Safe staining. A uniform intensity of bands and bright reference bands allow easy sizing and orientation. A defined amount of DNA in each band enables approximate quantification of sample DNA.

Novex Tris-Glycine Plus Midi Protein gels are improved polyacrylamide gels based on traditional Laemmli protein electrophoresis technology, allowing the use of Laemmli sample and running buffers. The gels offer reproducible separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine Plus Midi gels are a 1.0-mm thick, wider format (8 cm x 13 cm) gel for higher throughput electrophoresis of protein samples.

Learn more about our Novex Tris-Glcyine gels ›

View migration charts ›

Features of Novex Tris-Glycine Plus gels include:
Improved shelf life—store gels for up to 12 months at 4°C
Flexibility—use for native and denaturing protein assays
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine Plus Midi gels come in fixed concentrations of 10% and 12%, as well as gradient concentrations of 4–12%, 4–20%, and 8–16%. Select from our many well formats, including 12+2-, 20-, and 26-well.

Run your proteins in native or denatured form
Novex Tris-Glycine Plus gels do not contain SDS and so can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer. The gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion Cell using our adapters.

For transfer of proteins to a membrane, we recommend using the Novex Tris-Glycine Transfer Buffer when performing traditional wet transfer. Rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE gels for optimal results during day-to-day use.

Features of NuPAGE Bis-Tris gels:
• Better protein integrity—optimized sample preparation process preserves your proteins
• Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Learn more about all of our NuPAGE Bis-Tris gels >
View migration charts ›

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.

We also provide NuPAGE Tris-Acetate gels for separating larger proteins. For classic Laemmli-based Tris-glycine electrophoresis, we provide Novex Tris-Glycine gels.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer. Rapid semi-dry transfer can be done using the Pierce Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the XCell II Blot Module or the Mini Blot Module.

Related links
Overview of 1D Protein Electrophoresis
Comparison of NuPAGE Tris-Bis vs. traditional Tris-glycine gels

The Invitrogen PowerEase Touch 350W Power Supply is a high-throughput power supply with a 4.3-inch backlit LCD touchscreen. The touchscreen and user interface design make setting up custom protocols or selecting one of the several pre-programmed gel electrophoresis and transfer methods a breeze. This power supply is ideal for high current applications like high throughput DNA/RNA electrophoresis, SDS-PAGE, native PAGE, and western blotting. The PowerEase Touch 350W Power Supply offers four sets of output jacks that can be used simultaneously and three modes: constant voltage, constant current, and constant power for flexibility of use and efficiency.

See all available power supplies ›

Features:
• LCD touchscreen display and user interface with clear menu prompts for easy use by hand or stylus
• Advanced UI/UX design combined with 4.3-inch backlit LCD touchscreen for ease of use and convenience in monitoring run progress
• Maximum output of 300 V, 3 A (3000 mA), and 350 W
• Ideal for high-current applications like high throughput DNA/RNA electrophoresis, SDS-PAGE, native PAGE, and western blotting
• Four sets of output terminals allow running of multiple electrophoresis units
• Pop-up feet allow change to screen viewing angle
• Sturdy polyurethane feet and stackable housing design allow stacking of power supplies
• Running modes include constant voltage, constant current, or constant power
• Fully programmable power supply for programs with either constant voltage, current, or power
• Flexibility to either program a custom method (up to 100 custom methods, 20 steps per method, 999 minutes per step) or select one of several pre-programmed gel electrophoresis and transfer methods
• User adjustable device settings such as beep volume, screen brightness, and sleep mode duration
• Automatic safety features include No Load, Over Temperature, Over Voltage, Over Current, Load Change, and Ground Leak detection

This latest version of 3730xl DNA Analyzer has an upgraded solid-state laser, is 48- and 96-capillary compatible, and comes with Windows® 10 operating system and cloud connectivity. Like its predecessor, it is the gold standard for high-throughput genetic analysis. Use this analyzer for DNA fragment analysis applications such as microsatellites, AFLP, and SNP analysis, mutation detection, and traditional DNA sequencing. Get the highest quality data at a low cost per sample.

• Higher optical sensitivity and advanced polymers enable you to obtain higher-quality sequencing data for less
• Multiple automation features decrease costly human errors
• Optimized polymers increase your productivity without compromising your results
• Perform a wide variety of sequencing and fragment analysis applications including resequencing, microsatellite analysis, AFLP, LOH, SSCP, and SNP screening, and SNP validation

Large-scale DNA analysis on a small-scale budget
By combining advances in automation with innovative optics and proprietary reagents to increase throughput, yield high-quality data, and minimize reagent consumption, the 3730xl analyzer provides your laboratory and production facility with faster, better, and cheaper analysis.

Streamline your workflow
The 3730xl DNA Analyzer is engineered for highly reliable, unattended operation of up to 48 hours (applies to modules with run times longer than 30 minutes). To minimize the need for operator intervention and decrease the risk of human error, automation features include an integrated plate stacker, internal bar code reader, and onboard polymer delivery system.

Increase productivity
The high signal-to-noise ratio ensures you get high-quality data, even when you use low-concentration samples and reagents. Labor-saving automation features minimize hands-on time and enable you to analyze more data more efficiently.

Get the highest-quality sequencing and genotyping data
The enhanced optical design provides a higher signal-to-noise ratio and a more uniform signal profile across the array. This design, combined with our advanced polymer, enables the longest read lengths of any available system, and provides enhanced color balance for streamlined genotyping sample handling. In addition, exceptional sensitivity enables higher success rates across a wide range of sample template types and concentrations.

One instrument, multiple applications
With the 3730xl DNA Analyzer, you get the highest-quality data from a wide array of applications. In addition to being ideal for high-throughput sequencing, the analyzer's optimized application assays, instrument, and analysis software also provide a complete solution for genotyping and resequencing.

Integrated data analysis tools reduce time-to-results
The 3730xl DNA Analyzer software suite allows you to generate more meaningful data with less work. This system's labor-saving software suite includes:
• Data collection (supplied with the instrument)—manages your instrument setup, controls instrument operations, allows real-time data visualization, and performs diagnostics
• Sequencing analysis software—designed to base-call; assign quality values; trim, display, edit and print DNA sequencing data using the KB basecaller
• Seqscape Software—provides everything you need to perform resequencing applications such as VariantSEQr Resequencing System
• GeneMapper—enables configurable, automated allele calling; a plus for high-throughput genotyping and includes tools for SNPlex data analysis

Additional features include:
• Auto-analysis with GeneMapper and SeqScape software
• Flexibility to use any choice in dye set option
• Tools to assist with regulatory and compliance requirements (in the United States, this assists with FDA 21CFR part 11)
• Additional optimized run modules covering more applications
• Support for fragment analysis applications on the 96-capillary array

The instrument comes with a one year limited warranty on parts and labor.

Novex Tris-Glycine SDS Running Buffer (10X) is formulated for separation of proteins in their denatured state on Tris-Glycine gels. Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands.

Separate native or denatured proteins
Novex Tris-Glycine gels do not contain SDS and can be used to accurately separate both native and denatured proteins, depending upon the sample and running buffers used. To separate denatured proteins on Novex Tris-Glycine gels, use Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. To separate native proteins use Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

See all available buffers and reagents available for SDS-PAGE

The NuPAGE Tris-Acetate Welcome Pack provides all of the necessary NuPAGE Tris-Acetate gels, buffers, and reagents you will need to begin using the Mini Gel Tank.

The NuPAGE Tris-Acetate Welcome Pack contains:
• Mini Gel Tank
• NuPAGE Tris-Acetate mini gels (2 boxes, 20 gels)
• NuPAGE Tris-Acetate SDS Running Buffer (20X)
• NuPAGE LDS Sample Buffer (4X)
• NuPAGE Sample Reducing Agent (10X)
• HiMark Pre-stained Protein Standard

About the Mini Gel Tank
The Mini Gel Tank is compatible with all Invitrogen Novex, NuPAGE, and Bolt mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies used.

About NuPAGE Tris-Acetate gels
NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. NuPAGE Tris-Acetate gels are high performance polyacrylamide gels that simulate the denaturing or the native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of proteins compared to traditional Tris-glycine SDS-PAGE gels.

For transferring your proteins to a membrane, we recommend using for NuPAGE Transfer buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

The InVision His-tag In-gel Staining Kit includes InVision His-tag In-gel Stain as well as BenchMark His-tagged Protein Standard. InVision His-tag In-gel Stain is a ready-to-use, fast, sensitive, and highly specific stain for visualizing His-tagged fusion protein bands directly in a polyacrylamide gel following electrophoresis.

Features:
• Stain proteins fused to an oligohistidine sequence
• Eliminate western blotting—detect His-tagged proteins directly in the gel
• Detect nanogram levels of protein bands with a UV transilluminator or imaging equipment with appropriate filters or lasers
• Visualize results in under three hours

Compare all His-tag stains ›

Fast and easy staining
The InVision His-tag In-gel Stain protocol is easy to perform and requires very little hands-on-time, allowing for rapid and scalable protein expression screening in a variety of gel types. Following electrophoresis, the gel is fixed, incubated in the stain, washed, and the specific His-tagged fusion protein detected by exposing the gel to UV or visible light. In under three hours, nanogram levels of His-tagged fusion protein can be detected. After documenting your InVision Stain signal, total protein staining can be performed.

Thermo Scientific NoLimits is a collection of 36 highly-purified individual DNA fragments ranging from 10 to 20,000 bp. Unlike other vendors, where custom standards are produced by PCR, Thermo Scientific NoLimits DNA fragments are produced using specifically designed plasmids purified by patented technology.Plasmid DNA, digested with Thermo Scientific Restriction Enzymes, is chromatography-purified to prepare the individual DNA fragments from the digestion mixture. The results are extremely pure DNA fragments, free of any truncated or degraded molecules. These custom DNA fragments are the best custom standards for applications ranging from electrophoresis (both gel and capillary) to HPLC and beyond.

Features

• Broad range: 10-20,000 bp
• Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis
• Precise DNA concentration
• Chromatography-purified individual DNA fragments

Applications

• Gel electrophoresis
• Capillary electrophoresis
• DNA quantification
• HPLC analysis
• Applications where a custom DNA size standard is required.

Thermo Scientific GeneRuler 100 bp Plus DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 100 bp to 3,000 bp on agarose or polyacrylamide gels. The DNA ladder consists of 14 DNA fragments and is provided with 6X TriTrack DNA Loading Dye.

Highlights of 100 bp Plus DNA Ladder
• Easy visualization—three-color tracking of DNA migration during electrophoresis with 6X TriTrack DNA Loading Dye (contains Xylene Cyanol FF, Bromophenol Blue, and Orange G)
• Simplicity—one loading dye for a variety of samples
• Sharp bands—clear results with mixtures of individual chromatography-purified DNA fragments
• Convenient—ready-to-load DNA Ladder is premixed with 6X TriTrack DNA Loading dye and stable at room temperature for up to 6 months

Product use
This double-stranded DNA ladder is compatible with 1–2% standard and precast agarose slab gels and can be visualized after ethidium bromide or SYBR Safe staining. A uniform intensity of bands and bright reference bands allow easy sizing and orientation. A defined amount of DNA in each band enables approximate quantification of sample DNA.

5% Digitonin is a detergent solution used to prepare native protein samples for use with the NativePAGE™ Gel System. Also included in the NativePAGE™ Sample Prep Kit, this ready-to-use detergent solution improves solubility of hydrophobic and membrane proteins during sample preparation. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis showing increased resolution and reduced streaking.