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DNA Retardation Gels (6%), 1.0 mm, 15 well Invitrogen™
Formulation: Novex® TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS.
Recommended Buffers: For optimum performance, Novex® TBE Running Buffer and Novex® Hi-Density TBE Sample Buffer are strongly recommended for use with these gels. Novex® Hi-Density TBE Sample Buffer contains the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, and the tracking dyes Bromophenol Blue and Xylene Cyanol.

DNA Retardation Gels (6%), 1.0 mm, 10 well Invitrogen™
Formulation: Novex® TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS.
Recommended Buffers: For optimum performance, Novex® TBE Running Buffer and Novex® Hi-Density TBE Sample Buffer are strongly recommended for use with these gels. Novex® Hi-Density TBE Sample Buffer contains the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, and the tracking dyes Bromophenol Blue and Xylene Cyanol.

Novex™ WedgeWell™ Tris-Glycine Welcome Pack, 4 to 20%, 12-well Invitrogen™
The Novex Tris-Glycine Welcome Pack contains:
• Mini Gel Tank
• Novex Tris-Glycine Mini Gels, WedgeWell format (2 boxes, 20 gels)
• Novex Tris-Glycine SDS Running Buffer (10X)
• Novex Tris-Glycine SDS Sample Buffer (2X)
• NuPAGE Sample Reducing Agent (10X)
• PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa
About the Mini Gel Tank
The Mini Gel Tank is compatible with all Novex and NuPAGE mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies being used.
About Novex Tris-Glycine gels, WedgeWell format
Novex Tris-Glycine mini gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis, allowing the use of Laemmli sample and running buffers. They enable consistent performance and separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.
For transferring your proteins to a membrane, we recommend using for Novex Tris-Glycine Native Running Buffer or Novex Tris-Glycine Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Owl™ Gel Staining Box Thermo Scientific™
Stain, fix and de-stain in the same container with Thermo Scientific™ Owl™ Gel Staining Boxes, offering a convenient, conventional staining method. Boxes hold up to 125mL of liquid and one gel when used on a shaking platform.

Owl™ P82 Dual Gel System Replacement Parts Thermo Scientific™
Ensure flat, even banding and crisp resolution with Thermo Scientific™ Owl™ P82 Dual Gel System Replacement Parts. For use with the Owl™ P82 Dual Gel System, parts include replacement plates, gaskets and wedges.

Bolt™ 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 17-well Invitrogen™
Learn more about our Bolt Bis-Tris Plus gels >
View migration charts ›
Features include:
• Optimized run conditions—separate your proteins using constant voltage in approximately 35 minutes
• Long shelf life—store gels for at least 16 months at 4–25°C
• Better protein integrity—neutral pH sample preparation process preserves your proteins
• Wedge-shaped wells—for easier sample loading and up to two times higher sample volume
Choose the right Bolt Bis-Tris Plus gels for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and 4–12% gradient. The gels can be run using Bolt MES SDS or Bolt MOPS SDS running buffer to obtain different separation ranges. Bolt Bis-Tris Plus gels consist of a neutral pH-buffered (pH 6.4) polyacrylamide gel and have an operating pH of about 7.0. Bolt Bis-Tris Plus gels also come in four different well formats: 10, 12, 15, and 17 wells.
Convenient to use
Bolt Bis-Tris Plus gels can be run using constant voltage in approximately 35 minutes with the Bolt MES running buffer. Bolt Bis-Tris Plus gels offer sharper bands, high resolution, longer shelf life, and higher accuracy of results than Tris-glycine–based gels. In addition, Bolt Bis-Tris Plus gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0-mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier. And Bolt Bis-Tris Plus gels have a shelf life of at least 16 months at 4°C to 25°C.
For transferring your proteins to a membrane, we recommend using for Bolt Transfer buffer for traditional wet transfer using the Mini Blot Module . Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device .

A2 and A5 Large Gel System Replacement End Gates Thermo Scientific™

Owl™ A1 Large Gel Electrophoresis System Standard Comb Thermo Scientific™
Eliminate comb assembly with this assortment of heavy-duty, one-piece Thermo Scientific™ Owl™ A1 Large Gel Electrophoresis System Combs, for use with Owl A1 Large Gel Electrophoresis Systems.

Novex™ Tris-Glycine Welcome Pack, 10%, 10-well Invitrogen™
The Novex Tris-Glycine Welcome Pack contains:
• Mini Gel Tank
• Novex Tris-Glycine Mini Gels, WedgeWell format (2 boxes, 20 gels)
• Novex Tris-Glycine SDS Running Buffer (10X)
• Novex Tris-Glycine SDS Sample Buffer (2X)
• NuPAGE Sample Reducing Agent (10X)
• PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa
About the Mini Gel Tank
The Mini Gel Tank is compatible with all Novex and NuPAGE mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies being used.
About Novex Tris-Glycine gels, WedgeWell format
Novex Tris-Glycine mini gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis, allowing the use of Laemmli sample and running buffers. They enable consistent performance and separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.
For transferring your proteins to a membrane, we recommend using for Novex Tris-Glycine Native Running Buffer or Novex Tris-Glycine Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

GeneRuler Low Range DNA Ladder Thermo Scientific™
Highlights of Low Range DNA Ladder
• Easy visualization—three-color tracking of DNA migration during electrophoresis with 6X TriTrack DNA Loading Dye (contains Xylene Cyanol FF, Bromophenol Blue, and Orange G)
• Simplicity—one loading dye for a variety of samples
• Sharp bands—clear results with mixtures of individual chromatography-purified DNA fragments
Product use
This double-stranded DNA ladder is compatible with 3% standard and precast agarose slab gels and can be visualized after ethidium bromide or SYBR Safe staining. A uniform intensity of bands and bright reference bands allow easy sizing and orientation. A defined amount of DNA in each band enables approximate quantification of sample DNA.

Novex™ 10 to 20%, Tricine, 1.0 mm, Mini Protein Gel, 12-well Invitrogen™
Features of Novex Tricine protein gels:
• Increased resolution of proteins with molecular weights as low as 2 kDa
• Improved compatibility with direct sequencing of proteins after transferring to PVDF
• Minimized protein modification due to the lower pH of the tricine buffering system
Learn more about our Tricine gels ›
View migration charts ›
Formulation: Invitrogen Tricine gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. Our Tricine gels have a 4% stacking gel and do not contain SDS. The Tricine system requires SDS in sample and running buffers for best results.
Choose the right Tricine gel for your protein separation
Invitrogen Tricine gels come in three polyacrylamide concentrations of 10%, 16%, and a gradient of 10–20%. Select from our many well formats, including 10-, 12-, and 15-well. Tricine gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, we recommend Tricine SDS Sample Buffer and optimal separation use Tricine SDS Running Buffer.
For transfer of proteins to a membrane, we recommend using the Novex Tris-Glycine Transfer Buffer if performing a traditional wet transfer using the XCell II Blot Module or the Mini Blot Module. Rapid semi-dry transfer can be performed using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.
Related links
Specialized Protein Gel Support
Protein Gel 1D Electrophoresis Support

Novex™ WedgeWell™ Tris-Glycine Welcome Pack, 4 to 20%, 15-well Invitrogen™
The Novex Tris-Glycine Welcome Pack contains:
• Mini Gel Tank
• Novex Tris-Glycine Mini Gels, WedgeWell format (2 boxes, 20 gels)
• Novex Tris-Glycine SDS Running Buffer (10X)
• Novex Tris-Glycine SDS Sample Buffer (2X)
• NuPAGE Sample Reducing Agent (10X)
• PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa
About the Mini Gel Tank
The Mini Gel Tank is compatible with all Novex and NuPAGE mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies being used.
About Novex Tris-Glycine gels, WedgeWell format
Novex Tris-Glycine mini gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis, allowing the use of Laemmli sample and running buffers. They enable consistent performance and separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.
For transferring your proteins to a membrane, we recommend using for Novex Tris-Glycine Native Running Buffer or Novex Tris-Glycine Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

NativePAGE™ 4 to 16%, Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well Invitrogen™
Features of the NativePAGE Bis-Tris Gel System include:
• Wide molecular weight resolving range, from 15 – 10,000 kDa
• Neutral-pH separation, which better preserves the native state of protein complexes
• Resolution of all proteins in the gel regardless of their isoelectric point (pI)
• Ability to analyze membrane-protein complexes in their native conformations
• Ability to obtain better resolution than with traditional tris-glycine native electrophoresis
Learn more about the NativePAGE system ›
How NativePAGE Bis-Tris Gels work
In SDS-PAGE, SDS functions as a charge-shift molecule that denatures proteins by conferring on them a net negative charge and enables proteins to migrate towards the anode. BN PAGE uses Coomassie G-250 as the charge-shift molecule, which binds to proteins and confers a net negative charge while maintaining the proteins in their nondenatured, native state. The near-neutral pH of the NativePAGE Bis-Tris Gel System provides maximum stability of both the proteins and the gel matrix, resulting in a highly sensitive method for analysis of native membrane-protein complexes and offering superior band resolution over traditional Tris-glycine gel systems.
For transferring proteins to a membrane, we recommend using NuPAGE Transfer Buffers for traditional wet transfer and PVDF membranes. The NuPAGE transfer buffer maintains the neutral pH environment established during electrophoresis. Nitrocellulose membranes are not compatible for blotting since the membrane will bind the Coomassie G-250 dye very tightly. Alternatively, Rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device using PVDF membranes.

Bolt™ Bis-Tris Welcome Pack, 12%, 10-well Invitrogen™
• Mini Gel Tank
• Bolt Bis-Tris Plus gels (2 boxes, 20 gels)
• Bolt MES Running Buffer (20X)
• Bolt LDS Sample Buffer (4X)
• Bolt Sample Reducing Agent (10X)
• PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa
About the Mini Gel Tank
The Mini Gel Tank is compatible with Novex gels, NuPAGE gels, and Bolt Bis-Tris Plus gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt Bis-Tris Plus gels to be run in approximately 35 minutes. Run times may vary depending on buffer conditions and power supplies used.
About Bolt Bis-Tris Plus Gels
Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8, 10, 12, and 4-12% gradient and multiple well formats. Bolt Bis-Tris gels feature wedge-shaped wells, which can easily load up to two times more sample volume allowing for detection of proteins in very dilute samples or visualization of low-abundance proteins.
For transferring your proteins to a membrane, we recommend using for Bolt Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Novex™ Zymogram Developing Buffer (10X) Invitrogen™
Formulation: All Novex® Zymogram Gels are Tris-Glycine gels with a substrate (gelatin or casein) incorporated into the gel. They are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, highly purified water and casein or gelatin as the substrate. All have a 4% stacking gel.
Zymogram Gel Types: The 10% Zymogram (Gelatin) Gel is a 10% Tris-Glycine gel with 0.1% gelatin as the substrate. The 12% Novex® Zymogram (Casein) Gel is a 12% Tris-Glycine gel with 0.05% casein as the substrate. The 4-16% Novex® Zymogram (Blue Casein) Gel is a 4-16% Novex® Tris-Glycine gel with 0.1% casein as the substrate and a proprietary dye incorporated throughout the gel.
Sensitivity Level:
10% Zymogram Gelatin Gel: 5 x 10-6 units of collagenase
12% Zymogram Casein Gel: 7 x 10-4 units of trypsin
4-16% Zymogram Casein Gel: 1.5 x 10-3 units of trypsin
Recommended Buffers: Use Novex® Tris-Glycine SDS Sample Buffer and Novex® Tris-Glycine SDS Running Buffer with these gels. Novex® Zymogram Renaturing and Developing Buffers are also recommended.