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Owl™ EC300XL2 Compact Power Supply Thermo Scientific™
Retain settings after shut-down with the power-off memory of the Thermo Scientific™ Owl™ EC300XL2 Compact Power Supply. It is ideal for DNA/RNA and Protein electrophoresis.
Novex™ WedgeWell™ 8%, Tris-Glycine, 1.0 mm, Mini Protein Gel, 15-well Invitrogen™
Invitrogen Novex Tris-Glycine Mini Gels, WedgeWell format, are polyacrylamide gels that provide reproducible separation of a wide range of proteins into well-resolved bands with two times the loading capacity. Novex Tris-Glycine Mini Gels are based on traditional Laemmli protein electrophoresis that allow the use of Laemmli sample and running buffers.
Learn more about our Novex Tris-Glcyine gels ›
View migration charts ›
Features of Novex Tris-Glycine gels, WedgeWell format, include:
• Improved shelf life—store gels for up to 6 months at 4°C
• Diversity—use for native and denaturing protein assays
• Wedge-shaped wells—easily load up to two times more sample volume
• Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes
Choose the right gel format for your experiments
Novex Tris-Glycine gels, WedgeWell format, come in a variety of fixed concentrations from 6% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, and 10–20%. You can select from our many well formats, including 10-, 12-, and 15-well, and different pack sizes.
Run your proteins in native or denatured form
Novex Tris-Glycine gels, WedgeWell format, do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.
Convenient to use
Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.
For transferring your proteins to a membrane, we recommend using Novex Tris-Glycine Transfer Buffer when preforming traditional wet transfer using the Invitrogen Mini Blot Module. Transfers can also be performed with rapid semi-dry transfer using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.
Learn more about our Novex Tris-Glcyine gels ›
View migration charts ›
Features of Novex Tris-Glycine gels, WedgeWell format, include:
• Improved shelf life—store gels for up to 6 months at 4°C
• Diversity—use for native and denaturing protein assays
• Wedge-shaped wells—easily load up to two times more sample volume
• Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes
Choose the right gel format for your experiments
Novex Tris-Glycine gels, WedgeWell format, come in a variety of fixed concentrations from 6% to 18%, as well as gradients with ranges of 4–12%, 4–20%, 8–16%, and 10–20%. You can select from our many well formats, including 10-, 12-, and 15-well, and different pack sizes.
Run your proteins in native or denatured form
Novex Tris-Glycine gels, WedgeWell format, do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.
Convenient to use
Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.
For transferring your proteins to a membrane, we recommend using Novex Tris-Glycine Transfer Buffer when preforming traditional wet transfer using the Invitrogen Mini Blot Module. Transfers can also be performed with rapid semi-dry transfer using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.
Owl™ P8-Series System Combs Thermo Scientific™
Eliminate comb assembly with Thermo Scientific™ Owl™ P8-Series System Combs. This assortment of heavy-duty, one-piece combs is for use with the Owl™ P8-Series Electrophoresis System.
Pierce™ Silver Stain for Mass Spectrometry Thermo Scientific™
Pierce Silver Stain for Mass Spectrometry is a complete kit for rapid and ultra-sensitive silver staining of proteins in polyacrylamide gels and efficient destaining of excised gel pieces for mass spectrometry analysis. This kit enables both first-time and experienced users to achieve consistent and reliable staining using high-, low-, and gradient-percentage gels in 1D and 2D formats. The optimized staining method ensures extremely sensitive staining while minimizing covalent crosslinking of protein to the gel matrix, which can inhibit protein recovery. The destaining reagents facilitate complete removal of silver from stained protein bands and maximum protein recovery for subsequent mass spectrometry analysis.
Compare all silver stains ›
Features:
• Sensitivity—0.25 ng per spot in 30 minutes after fixing
• MS compatible—reagents and protocol are optimized to provide excellent performance in mass spectrometry following extraction of peptides from stained 1D or 2D gels (SDS-PAGE)
• Flexible staining protocol—fix gel for 15 to 30 minutes or overnight without any effect on staining performance; stain for 1 to 30 minutes (typically 2 to 3 minutes)
• Compatibility—effective silver stain for even difficult-to-stain basic proteins, such as lysozyme (pI 10) and chymotrypsinogen A (pI 9.2), which are detectable at 0.2 ng and 0.5 ng, respectively
• Complete kit—contains all reagents necessary for staining and destaining before in-gel proteolysis and peptide recovery for analysis by mass spectrometry
Silver staining methods generally use either glutaraldehyde or formaldehyde, which cause some covalent crosslinking of protein to each other and the gel matrix. To the extent that this crosslinking occurs, extraction or elution of protein from the gel will be inhibited. Pierce Silver Stain uses formaldehyde in the stain and developer working solutions. However, the procedure accompanying the kit for mass spectrometry is optimized to maximize polypeptide recovery without greatly sacrificing sensitivity.
Compare all silver stains ›
Features:
• Sensitivity—0.25 ng per spot in 30 minutes after fixing
• MS compatible—reagents and protocol are optimized to provide excellent performance in mass spectrometry following extraction of peptides from stained 1D or 2D gels (SDS-PAGE)
• Flexible staining protocol—fix gel for 15 to 30 minutes or overnight without any effect on staining performance; stain for 1 to 30 minutes (typically 2 to 3 minutes)
• Compatibility—effective silver stain for even difficult-to-stain basic proteins, such as lysozyme (pI 10) and chymotrypsinogen A (pI 9.2), which are detectable at 0.2 ng and 0.5 ng, respectively
• Complete kit—contains all reagents necessary for staining and destaining before in-gel proteolysis and peptide recovery for analysis by mass spectrometry
Silver staining methods generally use either glutaraldehyde or formaldehyde, which cause some covalent crosslinking of protein to each other and the gel matrix. To the extent that this crosslinking occurs, extraction or elution of protein from the gel will be inhibited. Pierce Silver Stain uses formaldehyde in the stain and developer working solutions. However, the procedure accompanying the kit for mass spectrometry is optimized to maximize polypeptide recovery without greatly sacrificing sensitivity.
Novex™ WedgeWell™ Tris-Glycine Welcome Pack, 8%, 10-well Invitrogen™
The Novex Tris-Glycine Welcome Pack provides all of the necessary Novex Tris-glycine gels, buffers, and reagents you need to begin using the Mini Gel Tank.
The Novex Tris-Glycine Welcome Pack contains:
• Mini Gel Tank
• Novex Tris-Glycine Mini Gels, WedgeWell format (2 boxes, 20 gels)
• Novex Tris-Glycine SDS Running Buffer (10X)
• Novex Tris-Glycine SDS Sample Buffer (2X)
• NuPAGE Sample Reducing Agent (10X)
• PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa
About the Mini Gel Tank
The Mini Gel Tank is compatible with all Novex and NuPAGE mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies being used.
About Novex Tris-Glycine gels, WedgeWell format
Novex Tris-Glycine mini gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis, allowing the use of Laemmli sample and running buffers. They enable consistent performance and separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.
For transferring your proteins to a membrane, we recommend using for Novex Tris-Glycine Native Running Buffer or Novex Tris-Glycine Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.
The Novex Tris-Glycine Welcome Pack contains:
• Mini Gel Tank
• Novex Tris-Glycine Mini Gels, WedgeWell format (2 boxes, 20 gels)
• Novex Tris-Glycine SDS Running Buffer (10X)
• Novex Tris-Glycine SDS Sample Buffer (2X)
• NuPAGE Sample Reducing Agent (10X)
• PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa
About the Mini Gel Tank
The Mini Gel Tank is compatible with all Novex and NuPAGE mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies being used.
About Novex Tris-Glycine gels, WedgeWell format
Novex Tris-Glycine mini gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis, allowing the use of Laemmli sample and running buffers. They enable consistent performance and separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.
For transferring your proteins to a membrane, we recommend using for Novex Tris-Glycine Native Running Buffer or Novex Tris-Glycine Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.
Owl™ EC-200XL High-Current Power Supply Thermo Scientific™
Retain settings after shut-down with the power-off memory of the Thermo Scientific™ Owl™ EC-200XL High-Current Power Supply. It is ideal for DNA, RNA and protein electrophoresis, as well as electroblotting.
Novex™ WedgeWell™ Tris-Glycine Welcome Pack, 8%, 12-well Invitrogen™
The Novex Tris-Glycine Welcome Pack provides all of the necessary Novex Tris-glycine gels, buffers, and reagents you need to begin using the Mini Gel Tank.
The Novex Tris-Glycine Welcome Pack contains:
• Mini Gel Tank
• Novex Tris-Glycine Mini Gels, WedgeWell format (2 boxes, 20 gels)
• Novex Tris-Glycine SDS Running Buffer (10X)
• Novex Tris-Glycine SDS Sample Buffer (2X)
• NuPAGE Sample Reducing Agent (10X)
• PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa
About the Mini Gel Tank
The Mini Gel Tank is compatible with all Novex and NuPAGE mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies being used.
About Novex Tris-Glycine gels, WedgeWell format
Novex Tris-Glycine mini gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis, allowing the use of Laemmli sample and running buffers. They enable consistent performance and separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.
For transferring your proteins to a membrane, we recommend using for Novex Tris-Glycine Native Running Buffer or Novex Tris-Glycine Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.
The Novex Tris-Glycine Welcome Pack contains:
• Mini Gel Tank
• Novex Tris-Glycine Mini Gels, WedgeWell format (2 boxes, 20 gels)
• Novex Tris-Glycine SDS Running Buffer (10X)
• Novex Tris-Glycine SDS Sample Buffer (2X)
• NuPAGE Sample Reducing Agent (10X)
• PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa
About the Mini Gel Tank
The Mini Gel Tank is compatible with all Novex and NuPAGE mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies being used.
About Novex Tris-Glycine gels, WedgeWell format
Novex Tris-Glycine mini gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis, allowing the use of Laemmli sample and running buffers. They enable consistent performance and separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.
For transferring your proteins to a membrane, we recommend using for Novex Tris-Glycine Native Running Buffer or Novex Tris-Glycine Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.
Novex™ TBE Gels, 6%, 10 well Invitrogen™
Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments are clearly resolved into sharp, tight bands. Novex® TBE gels are designed to run on the XCell SureLock® Mini-Cell.
Formulation
Novex® TBE gels are manufactured with high-purity Tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS.
Recommended buffers
For optimum performance, Novex® TBE Running Buffer and Novex® Hi-Density TBE Sample Buffer are strongly recommended for use with these gels. Novex® Hi-Density TBE Sample Buffer contains the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, as well as the tracking dyes Bromophenol Blue and Xylene Cyanol.
Formulation
Novex® TBE gels are manufactured with high-purity Tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS.
Recommended buffers
For optimum performance, Novex® TBE Running Buffer and Novex® Hi-Density TBE Sample Buffer are strongly recommended for use with these gels. Novex® Hi-Density TBE Sample Buffer contains the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, as well as the tracking dyes Bromophenol Blue and Xylene Cyanol.
NuPAGE™ 4 to 12%, Bis-Tris, 1.5 mm, Mini Protein Gel, 10-well Invitrogen™
Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE gels for optimal results during day-to-day use.
Features of NuPAGE Bis-Tris gels:
• Better protein integrity—optimized sample preparation process preserves your proteins
• Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature
Learn more about all of our NuPAGE Bis-Tris gels >
View migration charts ›
Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.
NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.
We also provide NuPAGE Tris-Acetate gels for separating larger proteins. For classic Laemmli-based Tris-glycine electrophoresis, we provide Novex Tris-Glycine gels.
For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer. Rapid semi-dry transfer can be done using the Pierce Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the XCell II Blot Module or the Mini Blot Module.
Related links
Overview of 1D Protein Electrophoresis
Comparison of NuPAGE Tris-Bis vs. traditional Tris-glycine gels
Features of NuPAGE Bis-Tris gels:
• Better protein integrity—optimized sample preparation process preserves your proteins
• Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature
Learn more about all of our NuPAGE Bis-Tris gels >
View migration charts ›
Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.
NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer and NuPAGE Sample Reducing Agent. Use NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer to better resolve small proteins or NuPAGE MOPS SDS Running Buffer to resolve medium- to large-size proteins.
We also provide NuPAGE Tris-Acetate gels for separating larger proteins. For classic Laemmli-based Tris-glycine electrophoresis, we provide Novex Tris-Glycine gels.
For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer. Rapid semi-dry transfer can be done using the Pierce Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the XCell II Blot Module or the Mini Blot Module.
Related links
Overview of 1D Protein Electrophoresis
Comparison of NuPAGE Tris-Bis vs. traditional Tris-glycine gels
Novex™ WedgeWell™ Tris-Glycine Welcome Pack, 10%, 12-well Invitrogen™
The Novex Tris-Glycine Welcome Pack provides all of the necessary Novex Tris-glycine gels, buffers, and reagents you need to begin using the Mini Gel Tank.
The Novex Tris-Glycine Welcome Pack contains:
• Mini Gel Tank
• Novex Tris-Glycine Mini Gels, WedgeWell format (2 boxes, 20 gels)
• Novex Tris-Glycine SDS Running Buffer (10X)
• Novex Tris-Glycine SDS Sample Buffer (2X)
• NuPAGE Sample Reducing Agent (10X)
• PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa
About the Mini Gel Tank
The Mini Gel Tank is compatible with all Novex and NuPAGE mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies being used.
About Novex Tris-Glycine gels, WedgeWell format
Novex Tris-Glycine mini gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis, allowing the use of Laemmli sample and running buffers. They enable consistent performance and separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.
For transferring your proteins to a membrane, we recommend using for Novex Tris-Glycine Native Running Buffer or Novex Tris-Glycine Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.
The Novex Tris-Glycine Welcome Pack contains:
• Mini Gel Tank
• Novex Tris-Glycine Mini Gels, WedgeWell format (2 boxes, 20 gels)
• Novex Tris-Glycine SDS Running Buffer (10X)
• Novex Tris-Glycine SDS Sample Buffer (2X)
• NuPAGE Sample Reducing Agent (10X)
• PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa
About the Mini Gel Tank
The Mini Gel Tank is compatible with all Novex and NuPAGE mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies being used.
About Novex Tris-Glycine gels, WedgeWell format
Novex Tris-Glycine mini gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis, allowing the use of Laemmli sample and running buffers. They enable consistent performance and separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.
For transferring your proteins to a membrane, we recommend using for Novex Tris-Glycine Native Running Buffer or Novex Tris-Glycine Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.
E-Holder™ Platform Invitrogen™
The E-Holder™ platform is designed with a spring-loaded mechanism to ensure that the E-Gel® 96 and E-Gel® 48 gel cassettes stay firmly in place during robotic loading. This allows reproducible placement and loading from one gel to the next without having to make adjustments to the robot loading software. In addition, the E-Holder™ platform allows you to load samples on one E-Gel® 96 or E-Gel® 48 gel while other gels are running in the mother or daughter E-Base™ combination (Figure 1).
Note: The E-Holder™ platform can hold either E-Gel® 96, E-Gel® 48, or E-PAGE™ 96 Gels.
Note: The E-Holder™ platform can hold either E-Gel® 96, E-Gel® 48, or E-PAGE™ 96 Gels.
Novex™ TBE-Urea Gels, 6%, 10 well Invitrogen™
Novex® TBE-Urea Gels are denaturing polyacrylamide gels that resolve single-stranded DNA oligos or RNA into sharp, distinct bands. These gels are optimized for the analysis and purification of products ranging from 20-800 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies, and Northern blot analysis. Novex® TBE-Urea gels are designed to run on the XCell SureLock™ Mini-Cell.
Formulation: Novex® TBE-Urea gels are made with high-purity reagents, including Tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, APS, and 7M urea. Strict quality control ensures that gels and buffers are DNase- and RNase-free.
Recommended Buffers: For optimum performance, Novex® TBE-Urea sample buffers and Novex® TBE Running Buffer are strongly recommended for use with these gels. For analytical applications, Novex® TBE-Urea Sample Buffer is recommended. This buffer contains urea, the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, and the tracking dyes Bromophenol Blue and Xylene Cyanol.
Formulation: Novex® TBE-Urea gels are made with high-purity reagents, including Tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, APS, and 7M urea. Strict quality control ensures that gels and buffers are DNase- and RNase-free.
Recommended Buffers: For optimum performance, Novex® TBE-Urea sample buffers and Novex® TBE Running Buffer are strongly recommended for use with these gels. For analytical applications, Novex® TBE-Urea Sample Buffer is recommended. This buffer contains urea, the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, and the tracking dyes Bromophenol Blue and Xylene Cyanol.
P2-CST Multiple Gradient Gel Caster Comb, 25 teeth Thermo Scientific™
Cast one to five gels simultaneously with the Fisherbrand™ P2-CST Multiple Gradient Gel Caster, one of the simplest devices to cast gradient gels.
E-Gel™ Agarose Gels with SYBR™ Safe DNA Gel Stain, 1% Invitrogen™
Invitrogen E-Gel precast agarose gels with SYBR Safe DNA gel stain are designed for fast, convenient, and safe DNA sample electrophoresis.Each E-Gel agarose gel cassette contains all components and stain required for efficient gel separation and analysis—just load your samples and run. E-Gel agarose gels are ideal for analyzing PCR products, restriction digests, plasmid preparations, or DNA fragment libraries.
Features of E-Gel agarose gels include:
• Instantaneous setup—ready for use when you are and no gel preparation or additional buffers required
• Safer choice—contains SYBR Safe DNA stain, a safer alternative to ethidium bromide
• High performance—detect 2–3 ng of sample DNA with a separation time as short as ~30 minutes
• In-process control—view DNA sample migration in real time
• Easy sample access—openable cassette design allows easy in-gel access for convenient DNA sample excision or transfer to membranes
• Better sample integrity—exploits blue-light excitation wavelength to minimize DNA damage, as opposed to UV
Safer option
Choose SYBR Safe DNA stained gels to minimize exposure to hazardous and mutagenic factors such as ethidium bromide or UV. Precast E-Gel agarose gels with SYBR Safe, in combination with blue-light trans-illumination, offer better sample integrity and a safer work environment.
Fast and convenient analysis
E-Gel agarose gels enable fast and high-performance analysis with a detection sensitivity of 2–3 ng of sample DNA per band and a separation time 2–3 fold faster than that of conventional pour-your-own gels. Each E-Gel agarose gel contains agarose, electrodes, SYBR Safe DNA stain, and a buffer-less ion exchange system packaged inside a dry disposable cassette. E-Gel technology does not required any gel or buffer preparation or gel straining steps. Just load your samples and run.
Live monitoring of DNA migration
E-Gel agarose gels are pre-stained with SYBR Safe DNA gel stain which has an excitation wavelength in the blue-light spectrum. When used on the E-Gel Power Snap Electrophoresis Device with integrated blue-light trans-illuminator, this enables safe real-time monitoring of DNA migration without causing a risk to the eyes or a risk of damaging the sample, as opposed to UV.
Easy access to the gel
The E-Gel cassettes are designed for convenient opening with a Gel Knife (Cat. No. EI9010), so that the gel inside can be accessed readily for excision of specific bands or transferred to a membrane for Southern blot analysis.
Nucleic acid markers
For optimal DNA analysis on E-Gel agarose gels we recommend the specially formulated E-Gel 1 Kb Plus Express DNA Ladder (Cat. No: 10488091) or E-Gel 50 bp DNA Ladder (Cat. No. 10488099) or E-Gel Ultra Low DNA Ladder (Cat. No. 10488096). Our high-quality ready-to-use ladders are formulated using individual chromatography-purified DNA fragments and are suitable for storage at room temperature.
Learn more about our E-Gel DNA ladders ›
Running and imaging devices
E-Gel agarose gels require the E-Gel Power Snap Electrophoresis System for gel running and viewing. The system consists of an electrophoresis device and a camera for fast and convenient E-Gel agarose gel separation and analysis.
Learn more about the E-Gel Power Snap Electrophoresis System ›
Available in a variety of gel formats
E-Gel agarose gels are available in variety of gel percentage, stain, and well formats. The 11-well E-Gel agarose gels with SYBR Safe are available in 1%, 2%, and 4% gel percentages. E-Gel agarose gel selection guide
Features of E-Gel agarose gels include:
• Instantaneous setup—ready for use when you are and no gel preparation or additional buffers required
• Safer choice—contains SYBR Safe DNA stain, a safer alternative to ethidium bromide
• High performance—detect 2–3 ng of sample DNA with a separation time as short as ~30 minutes
• In-process control—view DNA sample migration in real time
• Easy sample access—openable cassette design allows easy in-gel access for convenient DNA sample excision or transfer to membranes
• Better sample integrity—exploits blue-light excitation wavelength to minimize DNA damage, as opposed to UV
Safer option
Choose SYBR Safe DNA stained gels to minimize exposure to hazardous and mutagenic factors such as ethidium bromide or UV. Precast E-Gel agarose gels with SYBR Safe, in combination with blue-light trans-illumination, offer better sample integrity and a safer work environment.
Fast and convenient analysis
E-Gel agarose gels enable fast and high-performance analysis with a detection sensitivity of 2–3 ng of sample DNA per band and a separation time 2–3 fold faster than that of conventional pour-your-own gels. Each E-Gel agarose gel contains agarose, electrodes, SYBR Safe DNA stain, and a buffer-less ion exchange system packaged inside a dry disposable cassette. E-Gel technology does not required any gel or buffer preparation or gel straining steps. Just load your samples and run.
Live monitoring of DNA migration
E-Gel agarose gels are pre-stained with SYBR Safe DNA gel stain which has an excitation wavelength in the blue-light spectrum. When used on the E-Gel Power Snap Electrophoresis Device with integrated blue-light trans-illuminator, this enables safe real-time monitoring of DNA migration without causing a risk to the eyes or a risk of damaging the sample, as opposed to UV.
Easy access to the gel
The E-Gel cassettes are designed for convenient opening with a Gel Knife (Cat. No. EI9010), so that the gel inside can be accessed readily for excision of specific bands or transferred to a membrane for Southern blot analysis.
Nucleic acid markers
For optimal DNA analysis on E-Gel agarose gels we recommend the specially formulated E-Gel 1 Kb Plus Express DNA Ladder (Cat. No: 10488091) or E-Gel 50 bp DNA Ladder (Cat. No. 10488099) or E-Gel Ultra Low DNA Ladder (Cat. No. 10488096). Our high-quality ready-to-use ladders are formulated using individual chromatography-purified DNA fragments and are suitable for storage at room temperature.
Learn more about our E-Gel DNA ladders ›
Running and imaging devices
E-Gel agarose gels require the E-Gel Power Snap Electrophoresis System for gel running and viewing. The system consists of an electrophoresis device and a camera for fast and convenient E-Gel agarose gel separation and analysis.
Learn more about the E-Gel Power Snap Electrophoresis System ›
Available in a variety of gel formats
E-Gel agarose gels are available in variety of gel percentage, stain, and well formats. The 11-well E-Gel agarose gels with SYBR Safe are available in 1%, 2%, and 4% gel percentages. E-Gel agarose gel selection guide
NuPAGE™ Tris-Acetate Welcome Pack, 3-8%, 15-well Invitrogen™
The NuPAGE Tris-Acetate Welcome Pack provides all of the necessary NuPAGE Tris-Acetate gels, buffers, and reagents you will need to begin using the Mini Gel Tank.
The NuPAGE Tris-Acetate Welcome Pack contains:
• Mini Gel Tank
• NuPAGE Tris-Acetate mini gels (2 boxes, 20 gels)
• NuPAGE Tris-Acetate SDS Running Buffer (20X)
• NuPAGE LDS Sample Buffer (4X)
• NuPAGE Sample Reducing Agent (10X)
• HiMark Pre-stained Protein Standard
About the Mini Gel Tank
The Mini Gel Tank is compatible with all Invitrogen Novex, NuPAGE, and Bolt mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies used.
About NuPAGE Tris-Acetate gels
NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. NuPAGE Tris-Acetate gels are high performance polyacrylamide gels that simulate the denaturing or the native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of proteins compared to traditional Tris-glycine SDS-PAGE gels.
For transferring your proteins to a membrane, we recommend using for NuPAGE Transfer buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.
The NuPAGE Tris-Acetate Welcome Pack contains:
• Mini Gel Tank
• NuPAGE Tris-Acetate mini gels (2 boxes, 20 gels)
• NuPAGE Tris-Acetate SDS Running Buffer (20X)
• NuPAGE LDS Sample Buffer (4X)
• NuPAGE Sample Reducing Agent (10X)
• HiMark Pre-stained Protein Standard
About the Mini Gel Tank
The Mini Gel Tank is compatible with all Invitrogen Novex, NuPAGE, and Bolt mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies used.
About NuPAGE Tris-Acetate gels
NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. NuPAGE Tris-Acetate gels are high performance polyacrylamide gels that simulate the denaturing or the native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of proteins compared to traditional Tris-glycine SDS-PAGE gels.
For transferring your proteins to a membrane, we recommend using for NuPAGE Transfer buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.
