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Pierce™ Protein Concentrators PES, 30K MWCO, 0.5 mL Thermo Scientific™

Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting and buffer exchange of biological samples. This product has a molecular-weight cutoff of 30K, and can be used for processing sample volumes up to 0.5 mL.

Features of Pierce Protein Concentrators PES:

Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 10 minutes or less
Excellent protein recovery—unique design minimizes membrane fouling and provides high speed concentrations, even with particle laden solutions
Instrument-compatible—use standard microcentrifuge with fixed-angle rotors; collect and recover sample without invert spinning
Versatile—easy way to perform sample concentration, diafiltration or buffer-exchange of biological samples

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low non-specific binding and negligible protein aggregation on the membrane and provides reliable and consistent results for processing sample volumes between 100 and 500 µL. Typical protein recovery is 90% when the mass of the proteins in the sample is at least two-fold greater than the MWCO of the device used. Sample concentration of 10- to 30-fold can be achieved within 10 minutes.

Applications:
• Protein concentration with tissue culture media, antiserum or monoclonal antibody preparations
• Concentration of protein peaks following gel-permeation chromatography
• Removal of unincorporated label following protein modification reactions
• Concentration and desalt/buffer-exchange after eluting protein from ion-exchange, hydrophobic interaction (HIC), metal chelate or affinity chromatography columns
• Separating large molecules from small molecules

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least two-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.05 mg/mL.

Pierce Protein Concentrators PES allow accurate control of the dead-stop volume (15 µL) and final concentration factor, providing reliable and consistent sample processing. They can be used effectively at a maximum centrifugal force of 15,000 x g at room temperature. Centrifugal force, temperature and sample volume, concentration and viscosity affect filtration rate. Optimize centrifugal time for each application.

Related Products
Pierce™ Protein Concentrators PES, 10K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 3K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 100K MWCO, 0.5 mL

Pierce™ Protein Concentrators PES, 100K MWCO, 0.5 mL Thermo Scientific™

Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting and buffer exchange of biological samples. This product has a molecular-weight cutoff of 100K, and can be used for processing sample volumes up to 0.5 mL.

Features of Pierce Protein Concentrators PES:

Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 10 minutes or less
Excellent protein recovery—unique design minimizes membrane fouling and provides high speed concentrations, even with particle laden solutions
Instrument-compatible—use standard microcentrifuge with fixed-angle rotors; collect and recover sample without invert spinning
Versatile—easy way to perform sample concentration, diafiltration or buffer-exchange of biological samples

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low non-specific binding and negligible protein aggregation on the membrane and provides reliable and consistent results for processing sample volumes between 100 and 500 µL. Typical protein recovery is 90% when the mass of the proteins in the sample is at least two-fold greater than the MWCO of the device used. Sample concentration of 10- to 30-fold can be achieved within 10 minutes.

Applications:
• Protein concentration with tissue culture media, antiserum or monoclonal antibody preparations
• Concentration of protein peaks following gel-permeation chromatography
• Removal of unincorporated label following protein modification reactions
• Concentration and desalt/buffer-exchange after eluting protein from ion-exchange, hydrophobic interaction (HIC), metal chelate or affinity chromatography columns
• Separating large molecules from small molecules

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least two-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.05 mg/mL.

Pierce Protein Concentrators PES allow accurate control of the dead-stop volume (15 µL) and final concentration factor, providing reliable and consistent sample processing. They can be used effectively at a maximum centrifugal force of 15,000 x g at room temperature. Centrifugal force, temperature and sample volume, concentration and viscosity affect filtration rate. Optimize centrifugal time for each application.

Related Products
Pierce™ Protein Concentrators PES, 10K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 3K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 30K MWCO, 0.5 mL

Pierce™ Protein Concentrators PES, 50K MWCO, 0.5 mL Thermo Scientific™

Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular-weight cutoff (MWCO) of 50K, and can be used for processing sample volumes up to 0.5 mL.

Features of Pierce Protein Concentrators PES:
Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 10 minutes or less
Excellent protein recovery—unique design minimizes membrane fouling and provides high speed concentrations, even with particle laden solutions
Instrument-compatible—use standard microcentrifuge with fixed-angle rotors; collect and recover sample without invert spinning
Versatile—easy way to perform sample concentration, diafiltration, or buffer-exchange of biological samples

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low non-specific binding and negligible protein aggregation on the membrane and provides reliable and consistent results for processing sample volumes between 100 and 500 µL. Typical protein recovery is 90% when the mass of the proteins in the sample is at least two-fold greater than the MWCO of the device used. Sample concentration of 10- to 30-fold can be achieved within 10 minutes.

Applications:
• Protein concentration of tissue culture media, antiserum, or monoclonal antibody preparations
• Concentration of protein peaks following gel-permeation chromatography
• Removal of unincorporated label following protein modification reactions
• Concentration and desalt/buffer-exchange after eluting protein from ion-exchange, hydrophobic interaction (HIC), metal chelate, or affinity chromatography columns
• Separating large molecules from small molecules

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least two-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.05 mg/mL.

Pierce Protein Concentrators PES allow accurate control of the dead-stop volume (15 µL) and final concentration factor, providing reliable and consistent sample processing. They can be used effectively at a maximum centrifugal force of 15,000 x g at room temperature. Centrifugal force, temperature, and sample volume, concentration, and viscosity affect filtration rate. Optimize centrifugal time for each application.

Related products
Pierce Protein Concentrators PES, 3K MWCO, 0.5 mL
Pierce Protein Concentrators PES, 30K MWCO, 0.5 mL
Pierce Protein Concentrators PES, 100K MWCO, 0.5 mL

Pierce™ Protein Concentrators PES, 3K MWCO, 0.5 mL Thermo Scientific™

Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting and buffer exchange of biological samples. This product has a molecular-weight cutoff of 3K, and can be used for processing sample volumes up to 0.5 mL.

Features of Pierce Protein Concentrators PES:

Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 10 minutes or less
Excellent protein recovery—unique design minimizes membrane fouling and provides high speed concentrations, even with particle laden solutions
Instrument-compatible—use standard microcentrifuge with fixed-angle rotors; collect and recover sample without invert spinning
Versatile—easy way to perform sample concentration, diafiltration or buffer-exchange of biological samples

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low non-specific binding and negligible protein aggregation on the membrane and provides reliable and consistent results for processing sample volumes between 100 and 500 µL. Typical protein recovery is 90% when the mass of the proteins in the sample is at least two-fold greater than the MWCO of the device used. Sample concentration of 10- to 30-fold can be achieved within 10 minutes.

Applications:
• Protein concentration with tissue culture media, antiserum or monoclonal antibody preparations
• Concentration of protein peaks following gel-permeation chromatography
• Removal of unincorporated label following protein modification reactions
• Concentration and desalt/buffer-exchange after eluting protein from ion-exchange, hydrophobic interaction (HIC), metal chelate or affinity chromatography columns
• Separating large molecules from small molecules

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least two-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.05 mg/mL.

Pierce Protein Concentrators PES allow accurate control of the dead-stop volume (15 µL) and final concentration factor, providing reliable and consistent sample processing. They can be used effectively at a maximum centrifugal force of 15,000 x g at room temperature. Centrifugal force, temperature and sample volume, concentration and viscosity affect filtration rate. Optimize centrifugal time for each application.

Related Products
Pierce™ Protein Concentrators PES, 10K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 30K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 100K MWCO, 0.5 mL

2mL Nonsterile Centrifugal Filters Thermo Scientific™

Filter volumes up to 2mL with these Thermo Scientific™ National 2mL Nonsterile Centrifugal Filters. The filters feature a virgin polypropylene filter housing with tapered 5mL, capped receiver tube, and can be used with any benchtop or floor model laboratory centrifuge.

Pierce™ Protein Concentrators PES, 10K MWCO, 0.5 mL Thermo Scientific™

Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting and buffer exchange of biological samples. This product has a molecular-weight cutoff of 10K, and can be used for processing sample volumes up to 0.5 mL.

Features of Pierce Protein Concentrators PES:

Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 10 minutes or less
Excellent protein recovery—unique design minimizes membrane fouling and provides high speed concentrations, even with particle laden solutions
Instrument-compatible—use standard microcentrifuge with fixed-angle rotors; collect and recover sample without invert spinning
Versatile—easy way to perform sample concentration, diafiltration or buffer-exchange of biological samples

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low non-specific binding and negligible protein aggregation on the membrane and provides reliable and consistent results for processing sample volumes between 100 and 500 µL. Typical protein recovery is 90% when the mass of the proteins in the sample is at least two-fold greater than the MWCO of the device used. Sample concentration of 10- to 30-fold can be achieved within 10 minutes.

Applications:
• Protein concentration with tissue culture media, antiserum or monoclonal antibody preparations
• Concentration of protein peaks following gel-permeation chromatography
• Removal of unincorporated label following protein modification reactions
• Concentration and desalt/buffer-exchange after eluting protein from ion-exchange, hydrophobic interaction (HIC), metal chelate or affinity chromatography columns
• Separating large molecules from small molecules

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least two-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.05 mg/mL.

Pierce Protein Concentrators PES allow accurate control of the dead-stop volume (15 µL) and final concentration factor, providing reliable and consistent sample processing. They can be used effectively at a maximum centrifugal force of 15,000 x g at room temperature. Centrifugal force, temperature and sample volume, concentration and viscosity affect filtration rate. Optimize centrifugal time for each application.

Related Products
Pierce™ Protein Concentrators PES, 3K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 30K MWCO, 0.5 mL
Pierce™ Protein Concentrators PES, 100K MWCO, 0.5 mL
Benchtop Centrifuges

Pierce™ Protein Concentrator PES, 100K MWCO, 2-6 mL Thermo Scientific™

Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 100K and can be used for processing sample volumes ranging from 2 to 6 mL.

Features of Pierce Protein Concentrators PES:

• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.

Pierce™ Protein Concentrator PES, 3K MWCO, 2-6 mL Thermo Scientific™

Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 3K and can be used for processing sample volumes ranging from 2 to 6 mL.

Features of Pierce Protein Concentrators PES:

• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.

Nalgene™ Rapid-Flow™ Sterile Single Use Vacuum Filter Units Thermo Scientific™

Increase filtration efficiency. Thermo Scientific; Nalgene™ Nalgene; Nalgene™ Rapid-Flow; Nalgene™ disposable Filter Units feature the exclusive Rapid-Flow support column design, providing fast flow rates and high throughput. The last line of defense against cell culture contamination—Nalgene Rapid Flow Filter Units and Bottle Tops are the best choice for the filtration of cell culture media, serum, additives and buffers.

Target2™ Cellulose Acetate Syringe Filters Thermo Scientific™

Adding a syringe filtration step prior to injection not only helps to ensure more consistent and reliable results; it also helps protect delicate instruments and prolongs column life. Available in sizes and membrane types to meet any application need, Thermo Scientific™ Target2™ Syringe Filters provide cleaner sample extracts by removing interfering materials and fine particles. Target2 Cellulose Acetate Syringe Filters are a good choice for filtration of tissue culture media, or for filtration of sensitive biological samples.

Pierce™ Protein Concentrator PES, 3K MWCO, 5-20 mL Thermo Scientific™

Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 3K and can be used for processing sample volumes ranging from 5 to 20 mL.

Features of Pierce Protein Concentrators PES:

• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.

Pierce™ Protein Concentrator PES, 5K MWCO, 20-100 mL Thermo Scientific™

Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 5K and can be used for processing sample volumes ranging from 20 to 100 mL.

Features of Pierce Protein Concentrators PES:

• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)
• Excellent protein recovery—typical protein recovery is >90%
• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples
• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinning

For easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.

Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.

Target2™ Nylon Syringe Filters Thermo Scientific™

Adding a syringe filtration step prior to injection not only helps to ensure more consistent and reliable results; it also helps protect delicate instruments and prolongs column life. Available in sizes and membrane types to meet any application need, Thermo Scientific™ Target2™ Syringe Filters provide cleaner sample extracts by removing interfering materials and fine particles down to the rated pore size. Target2 Nylon Syringe Filters are a good choice for most general laboratory filtration, as well as filtration of most HPLC samples.

Nalgene™ Membrane and Prefilter Disks Thermo Scientific™

Rely on Thermo Scientific™ Nalgene™ Membrane and Prefilter Disks to meet a wide range of laboratory filtration needs. Used in conjunction with either single use or reusable hardware and filter holders, filter disks offer you flexibility and choice to fit many applications. Sterile filter disks are available in materials ranging from nylon, cellulose nitrate, cellulose acetate and glass fiber membranes.

Target2™ PTFE Syringe Filters Thermo Scientific™

Adding a syringe filtration step prior to injection not only helps to ensure more consistent and reliable results; it also helps protect delicate instruments and prolongs column life. Available in sizes and membrane types to meet any application need, Thermo Scientific™ Target2™ Syringe Filters provide cleaner sample extracts by removing interfering materials and fine particles. Target2 PTFE Syringe Filters are used for general biological filtration. They are especially well-suited for applications where high protein recovery is desired.
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