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Variable Compensation Condensers Thermo Scientific™

The Thermo Scientific™ Variable Compensation Condensers correct for spherical aberration caused by infrared windows beneath the sample (up to 3mm in thickness).

Variable Compensation Objectives Thermo Scientific™

The Thermo Scientific™ Variable Compensation Objectives correct for spherical aberration caused when infrared windows are placed above the sample (up to 3mm in thickness), or when the Micro Compression Cell (Part No. 0045-434) is used.

EVOS™ 4X Objective, fluorite, LWD

This fluorite objective is ideal for fluorescence and demanding transmitted-light applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 4X
• Numerical Aperture: 0.13
• Working Distance: 19.7 mm

Image Quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective Classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield Contrast versus Phase Contrast Objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas. Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long Working Distance versus Coverslip-Corrected Objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS Objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

EVOS™ Light Cube, CFP-YFP EM

All EVOS fluorescence imaging systems and the Countess II FL Automated Cell Counter contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS light cubes are user-changeable and automatically recognized by the system. The CFP-YFP EM light cube is ideal for use with CFP-YFP FRET emission and other fluorophores with excitation and emission maxima near 445 and 542 nm.

EVOS light cubes offers you these important advantages:

• A shorter light path that affords superior detection of fluorescent signals
• Continuous illumination that enables consistent results
• A 50,000+ hour bulb lifetime that affords lower operating costs
• Adjustable light intensity that helps reduce photobleaching

Revolutionary Light Path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Continuous Light Intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS systems have continuous light cube intensity, users can rely on consistent illumination and can compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching.

Less Expensive to Own and Maintain
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

EVOS Hard-coated Filter Sets for Higher Transmission Efficiencies
Hard-coated filter sets are more expensive that soft-coated filters, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS systems' hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.

To select the light cube best suited for your experiments, see the Light Cube selection guide.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ 10X Objective, fluorite, LWD

This fluorite objective is ideal for fluorescence and demanding transmitted-light applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 10X
• Numerical Aperture: 0.30
• Working Distance: 8.3 mm

Image Quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective Classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield Contrast versus Phase Contrast Objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long Working Distance versus Coverslip-Corrected Objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS Objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

Nicolet™ iN™ 5 FTIR Microscope

Quickly identify unknown materials and contaminants that may affect product quality. The Thermo Scientific™ Nicolet™ iN™5 FTIR microscope provides rapid identification with point-and-shoot simplicity. The no-nonsense workflow requires only a few short steps; there’s no need to learn complex software. You can get answers in minutes — without the need for spectroscopy expertise. Simply see it, scan it, and solve it. Built for the demands of the busy QA/QC lab, the Nicolet iN5 microscope will provide reliable operation for years, across multiple users and product analysis needs.

Micro Compression Cell and Diamond™ Window Option Thermo Scientific™

The Micro Compression Cell provides a faster way to prepare microscope samples for transmission analysis by Fourier transform infrared (FT-IR) spectroscopy. The sample holder is used to flatten and crush samples for efficient analysis.

EVOS™ Light Cube, AO

All EVOS fluorescence imaging systems and the Countess II FL Automated Cell Counter contain our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light path that delivers superior fluorophore excitation. Each cube contains an LED, condensing optics, and hard-coated filters. EVOS light cubes are user-changeable and automatically recognized by the system. The AO light cube is ideal for use with Acridine orange, Alexa Fluor 430, Di-8 ANEPPS, DiA and other fluorophores with excitation and emission maxima near 442 and 488 nm.

EVOS light cubes offers you these important advantages:

• A shorter light path that affords superior detection of fluorescent signals
• Continuous illumination that enables consistent results
• A 50,000+ hour bulb lifetime that affords lower operating costs
• Adjustable light intensity that helps reduce photobleaching

Revolutionary Light Path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.

Continuous Light Intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS systems have continuous light cube intensity, users can rely on consistent illumination and can compare quantitative results from images acquired on different days. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching.

Less Expensive to Own and Maintain
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. This translates to savings in the overall upkeep of your instrument.

EVOS Hard-coated Filter Sets for Higher Transmission Efficiencies
Hard-coated filter sets are more expensive that soft-coated filters, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS systems' hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.

To select the light cube best suited for your experiments, see the Light Cube selection guide.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ 4X Objective, fluorite, LWD, 0.13NA/10.58WD Invitrogen™

This fluorite objective is ideal for fluorescence and demanding transmitted-light applications. This is a long working distance (LWD) objective that is optimized for the imaging of slides, cell culture dishes and flasks, and microtiter plates. All EVOS objectives offer outstanding optical performance from visible light to near infrared light. The extensive choice of objectives satisfies needs across the spectrum of magnifications and optical specifications.

Additional characteristics of this EVOS objective:

• Magnification: 4X
• Numerical Aperture: 0.13
• Working Distance: 10.58 mm

Image quality
Microscope objectives may be the most important components of an optical microscope because they are responsible for primary image formation. Image quality is crucial to experimental success and a requirement for publication; EVOS objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture perfection. EVOS objectives have the same or better numerical apertures as any other manufacturer's in the same class, and the broad selection means you have choices for your imaging requirements.

Objective classes

Achromat objectives are perfect for general applications, with standard correction of color and focus.

Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging.

Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light.

Brightfield contrast versus phase contrast objectives
Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas.

Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast).

Long working distance versus coverslip-corrected objectives
Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9-1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives.

For additional choices, visit the EVOS objectives selection guide
Explore the entire EVOS line of imaging systems and accessories

CapSure™ HS LCM Caps (Alignment Tray & Incubation Block required, not included) Applied Biosystems™

Specially designed for use with the ARCTURUS™ Laser Capture Microdissection (LCM) instruments, CapSure™ HS and CapSure™ Macro LCM Caps enable the precise and rapid extraction of pure populations of cells from heterogeneous sample preparations. CapSure Caps may be used to isolate individual cells or thousands of cells on a single cap.

Key product features:
• Gentle application – non-damaging microdissection preserves the integrity of the captured material
• Versatile extraction – capture both large and small amounts of cells

Ensure the Integrity of Microdissected Cells for Downstream Molecular Analysis
All CapSure™ LCM Caps have a patented transfer film bonded to the lower cap surface. Using the ARCTURUS™ Microdissection Systems an infrared laser pulses through the top of the cap during LCM and interacts with the transfer film, which then melts and bonds to the cells or regions of interest. The film absorbs the laser radiation – instead of the tissue or cell sample – creating a gentle, non-damaging microdissection that preserves the integrity of the captured material.

Extract Large Amounts of Cells with CapSure™ Macro LCM Caps
CapSure™ Macro LCM Caps provide the maximum area for capturing several thousand cells on a single cap. This is particularly useful for downstream applications that require large numbers of cells to obtain enough material for analysis, such as some protein analysis techniques. The CapSure™ Macro LCM Caps fit directly onto 0.5 ml microcentrifuge tubes for extraction of biological molecules from captured cells.

Extract Small Numbers of Cells with High Sensitivity CapSure™ HS Caps
CapSure™ HS LCM Caps enable the highly sensitive extraction and detection of biological molecules from small numbers of cells. CapSure™ HS LCM Caps contain ridges at the bottom that keep the active capture area of the cap twelve microns off the surface of the sample. This separation ensures the precise capture of up to several hundreds of cells. In addition, the CapSure™ LCM Caps work with ExtracSure devices that minimize the dilution of biological molecules extracted from captured cells. This optimizes the recovery of these molecules for downstream molecular analysis.

For Research Use Only. Not for use in diagnostics procedures.

EVOS™ Vessel Holder, SPL T-75 flask

EVOS vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS imaging systems for increased precision in sample alignment. This particular vessel holder accommodates one SPL T-75 flask. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

Note: an adapter is needed for use of this holder on the EVOS FL Auto automated stage.

For additional choices, see the EVOS Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS line of imaging systems and accessories.

15X Cassegrain Objectives (Fixed Compensation) Thermo Scientific™

The Thermo Scientific™ 0.58 N.A., 15X Cassegrain objectives with fixed compensation come in three compensation configurations—zero, 1mm, or 2mm compensation objectives.

EVOS™ Vessel Holder for Automated Stage, two 25 x 75 mm slides

EVOS vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS imaging systems for increased precision in sample alignment. This particular vessel holder securely holds two 25 x 75 mm standard or chamber microscope slides and is specific for the automated stage of the EVOS FL Auto Imaging System. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

For additional choices, see the EVOS Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ Stage Plate Adapter

The EVOS Stage Plate Adapter is designed to fit into the EVOS Staging Plate with 160 X 110 mm opening. This adapter accomodates vessel holders AMEPVH001 through AMEPVH018 for precision in sample alignment. With a snug and secure fit, yet readily switched, the plate/adapter combination make imaging samples in a range of vessel holders convenient and easy.

For additional choices, see the EVOS Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS line of imaging systems and accessories.

Richard-Allan Scientific™ Resolve™ Immersion Oil Thermo Scientific™

Eliminate toxicity and disposal problems with the Thermo Scientific™ Richard-Allan Scientific™ Resolve™ Immersion Oil, which contains no PCBs. It is a clear, colorless oil that allows light transmission throughout most of the UV-VIS spectrum.
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