Shop All Fluorometers and Fluorescence Polarization Systems

Qubit™ Flex Assay Tube Strips Invitrogen™

Qubit Flex Assay Tube Strips are strips of 8 x 200-µL thin-walled polypropylene tubes with very low fluorescence ideal for optimal performance of the Qubit Flex Fluorometer.

Qubit™ Flex Assay Reservoirs Invitrogen™

Qubit Flex Assay Reservoirs facilitate repeated multi-channel pipetting of Qubit reagents into Qubit Flex Assay Tube Strips for use in the Qubit Flex Fluorometer.

Qubit™ RNA IQ Assay Kit Invitrogen™

The Qubit RNA IQ Assay Kit provides a fast, simple method to check whether an RNA sample has degraded using a Qubit 4 or Qubit Flex fluorometer. The assay utilizes two unique dyes: one that binds to large, intact and/or highly structured RNA (mRNA, tRNA, rRNA), and another that selectively binds to small and/or, degraded RNA. Together they enable you to quickly assess the quality and integrity of an RNA sample.

To use, simply add your samples to the RNA IQ working solution, then measure on a Qubit 4 or Qubit Flex fluorometer. Results are presented as an RNA IQ number (RNA IQ#) that indicates the RNA sample integrity and quality, and also as a calculated percent of large and small RNA in the sample. The RNA IQ# is a value from 1 to 10, similar to other RNA quality scores, where a small number indicates that the sample is comprised of mainly small RNA and a larger number indicates that the sample consists of mainly large RNA or RNA with tertiary structure.

Solution-based compared to electrophoresis methods
The RNA IQ assay results correlate well with electrophoretic methods. The primary difference between the solution-based RNA IQ assay and traditional electrophoresis-based techniques is the amount of time it takes to ascertain whether an RNA sample has degraded. Gels are considered easy to perform, but still take time to run. Microfluidic-based methods not only take time to obtain results, but often have lengthy procedures to prepare the samples. Gel or microfluidic-based electrophoresis is still recommended to obtain fragment size information or size distribution of the RNA sample.

Notes:
1. The Qubit RNA IQ Assay will only work on a Qubit 4 or Qubit Flex fluorometer. The assay is not designed for use with Qubit, Qubit 2.0, or Qubit 3 fluorometers.
2. Use thin-wall, clear, 0.5-mL PCR tubes (Cat. No. Q32856) for the Qubit 4 Fluorometer and 8 x 200-µL tube strips (Cat. No. Q33252) for the Qubit Flex Fluorometer.

Qubit™ Flex Quantitation Starter Kit Invitrogen™

The Qubit Flex Quantitation Starter Kit includes:
• Qubit Flex Fluorometer (Cat. No. Q33326)
• Qubit Flex Assay Tube Strips (125 strips) (Cat. No. Q33252)
• Qubit 1X dsDNA HS Assay Kit (100 assays) (Cat. No. Q33230)
• Qubit dsDNA BR Assay Kit (100 assays) (Cat. No. Q32850)
• Qubit RNA HS Assay Kit (100 assays) (Cat. No. Q32852)
• Qubit RNA BR Assay Kit (100 assays) (Cat. No. Q10210)
• Qubit Protein Assay Kit (100 assays) (Cat. No. Q33211)
• Qubit Flex System Verification Assay Kit (Cat. No. Q33254)
• Qubit Flex Reservoirs (100 reservoirs) (Cat. No. Q33253)
• WiFi Dongle (Cat. No. A26774)
Qubit Flex SmartStart Digital Educational Experience—includes interactive visual demonstrations of how to install, operate, and maintain your instrument
Three-year Extended Warranty—if your instrument needs repair we will send you a factory-certified refurbished replacement

The Qubit Flex Fluorometer is a benchtop fluorometer designed for highly accurate quantification of DNA, RNA, microRNA and protein. With the Qubit Flex Fluorometer you have the flexibility to directly measure fluorescence of up to eight samples simultaneously, which reduces assay variability. Paired with Qubit reagents, the Qubit Flex Fluorometer is part of an optimized workflow generating highly accurate and reproducible results. The fluorometer has a large screen and intuitive user interface making it easy to select and run your desired assay with results displayed in just a few seconds.

For all Qubit assays, the concentration of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants on the result. Qubit assays for DNA, RNA, and protein quantitation are included in this quantitation starter kit.

This starter kit also includes a Qubit Flex System Verification Assay Kit which is a fast, easy-to-use, reagent-based assay that tests the performance of the Qubit Flex Fluorometer.

Qubit™ RNA XR Standard Invitrogen™

The Qubit RNA XR (extended range) Assay RNA standard is designed for use with Qubit RNA XR Assay Kit. This standard can also be used with the Quant-iT RNA XR Assay Kit (Cat. No. Q33225). It is supplied at the same concentration as the highest concentration RNA standard in the Quant-iT assay and is simply diluted into the standard series. This standard cannot be used as a replacement for the large and/or structured standard in the Qubit RNA IQ Assay. Sufficient material is supplied to perform standards with up to 500 samples.

Note: Use thin-wall, clear, 0.5-mL PCR tubes (Cat. No. Q32856) for the Qubit 4 fluorometer and 8 x 200-µL tube strips (Cat. No. Q33252) for the Qubit Flex fluorometer.

Qubit™ Flex System Verification Assay Kit Invitrogen™

The Qubit Flex System Verification Assay Kit is a fast, easy-to-use, reagent-based assay that tests the performance of the Qubit Flex Fluorometer. The kit consists of three components: a blank reagent solution, a green fluorescent reagent, and a far-red fluorescent reagent. The green fluorescent reagent is designed to test blue excitation (470 nm LED; 430-495 nm excitation filter) and green fluorescence detection (510-580 nm emission filter). The far-red fluorescent reagent is designed to test red excitation (635 nm LED; 600-645 nm excitation filter) and far-red fluorescence detection (665-720 nm emission filter).

Note: The Qubit Flex System Verification Assay Kit can only be used with the Qubit Flex Fluorometer.

Qubit™ 4 System Verification Assay Kit Invitrogen™

The Qubit 4 System Verification Assay Kit is a fast, easy-to-use, reagent-based assay that tests the performance of the Qubit 4 Fluorometer. The kit consists of three components: a blank reagent solution, a green fluorescent reagent, and a far-red fluorescent reagent. The green fluorescent reagent is designed to test the blue excitation (470 nm LED; 430-495 nm excitation filter) and green fluorescence detection (510-580 nm emission filter) on the fluorometer, while the far-red fluorescent reagent is designed to test the red excitation (635 nm LED; 600-645 nm excitation filter) and far-red fluorescence detection (665-720 nm emission filter).

The assay kit provides sufficient reagents to perform up to 50 assays.

Note: The Qubit 4 System Verification Assay Kit can only be used with the Qubit 4 Fluorometer.

Quant-iT™ RNA XR Assay Kit Invitrogen™

The Quant-iT RNA XR (Extended Range) Assay Kit provides an accurate and selective method for the quantitation of high-abundance RNA samples. The assay is highly selective for RNA and does not quantitate DNA, protein, or free nucleotides. Common contaminants, such as salts, free nucleotides, solvents, detergents, and protein, are well-tolerated in the assay. The assay kit is designed to be accurate for initial RNA sample concentrations of 10 ng/µL to 10,000 ng/µL depending on sample volume, providing a linear detection range of 200–10,000 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add the sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using a fluorescent-based microplate reader.

Qubit™ 4 RNA IQ Starter Kit, with WiFi Invitrogen™

The Qubit 4 RNA IQ Starter Kit includes:
• One Qubit 4 Fluorometer (Cat. No. Q33226)
• Qubit Assay Tubes (set of 500) (Cat. No. Q32856)
• WiFi Dongle (Cat. No. A26774)
• Qubit RNA IQ Assay Kit, 275 assays (Cat. No. Q33222)

The Qubit 4 Flurometer with WiFi is the latest version of the popular Qubit fluorometer designed to accurately measure DNA, RNA, and protein quantity, and now also RNA integrity and quality, using the highly sensitive Qubit assays. The Qubit 4 Fluorometer was re-engineered to enable data transfer via WiFi as well as to run the Qubit RNA IQ (integrity & quality) assay. The Qubit 4 Fluorometer and RNA IQ Assay Kit work together to accurately distinguish intact from degraded RNA in just two easy steps.

For all Qubit assays, the concentration or quality of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants on the result. The easy-to-use touch-screen menus make it easy to select and run the assays you need, with results displayed in just a few seconds.

Key features of the Qubit 4 Fluorometer include:
• Fast and highly accurate quantitation of DNA, RNA, and protein in less than three seconds per sample
• Measures intact RNA in less than 5 seconds per sample
• High levels of accuracy using only 1–20 μL of sample, even with very dilute samples
• On-board Reagent Calculator quickly generates Qubit working solution preparation instructions
• Stores results from up to 1000 samples
• Large 5.7-inch, state-of-the-art color touch screen for easy workflow navigation
• Graphical display indicates when samples are in the extended range or out of range
• Small footprint saves space on your bench
• Flexibility to export data to using the included WiFi dongle, a USB drive, or directly to your computer via a USB cable
• Ability to personalize your Qubit fluorometer with the assays you run most, add new assays, or even create your own assays with the MyQubit software and web tool
• Language of your choice including English, French, Spanish, Italian, German, simplified Chinese, and Japanese
• Pre-loaded program to test performance of fluorometer using the Qubit Fluorometer Verification Assay Kit

Learn more about the Qubit Fluorometer ›

The Qubit 4 Flurometer can also be used to directly measure the fluorescence of samples using Fluorometer mode. Additionally, Ion Sphere Particle quality can be evaluated on the Qubit 4 Fluorometer using the Ion Sphere Quality Control Kit prior to performing a sequencing run on the Ion PGM sequencer.

Enables more accurate results than with UV absorbance at low concentrations
The Qubit 4 Fluorometer is far more sensitive than UV absorbance, which measures anything absorbing at 260 nm, including DNA, RNA, protein, free nucleotides, or excess salts. Moreover, UV spectrophotometry is often not sensitive enough to accurately measure low concentrations of DNA and RNA. With the Qubit fluorometer, your research is enhanced by more accurate measurements because the dyes in the Qubit assay kits fluoresce only when bound to the selected molecule (DNA, RNA or protein) in your sample. This allows you to avoid repeating work due to inaccurate measurements.

Fast, simple, and accurate measurement of RNA quality
The Qubit RNA IQ assay provides a fast, simple method to check whether an RNA sample has degraded. The assay utilizes two unique dyes: one that binds to large, intact RNA, and another that selectively binds to small, degraded RNA. Together they enable you to quickly assess the quality and integrity of an RNA sample. To use, simply add your samples to the RNA IQ working solution, then measure on the Qubit 4 Fluorometer.

Save samples and bench space
The thoughtfully designed Qubit 4 Fluorometer requires samples of only 1–20 µL. It is intended for use at room temperature and occupies only a small amount of bench space. The Qubit 4 Fluorometer features advanced optics and data analysis algorithms, and comes equipped with a WiFi dongle, a USB thumb drive, and cable for data transfer to Excel™ software and for software downloads, as well as a universal power supply, four plug adapters, and electromagnetic CE certification. The Qubit 4 Fluorometer enables maximum flexibility for data export using WiFi, USB drive, or USB cable direct to computer. Additionally, all data can be saved in the Qubit cloud application, including direct transfer from the instrument via WiFi.

Single sample measurements are a breeze
This user-friendly fluorometer is combined with quick and simple assay procedures. Calculations and settings are automatically performed by the instrument. The Qubit assays for use with the Qubit 4 Fluorometer are all performed using the same general protocol, which uses a simple mix-and-read format with incubation times of only two minutes for the DNA and RNA assays. 500 L thin-walled PCR tubes (Cat. No. Q32856) are required.

NanoDrop™ 3300 Fluorospectrometer Thermo Scientific™

Choose the versatile, cost-effective Thermo Scientific™ NanoDrop 3300 Fluorospectrometer for broad spectrum fluorescence analysis. Using a patented technology, it uses as little as 1 μL of sample volume.

Qubit™ Flex NGS Starter Kit Invitrogen™

The Qubit Flex NGS Starter Kit includes:
• Qubit Flex Fluorometer (Cat. No. Q33326)
• Qubit Flex Assay Tubes Strips (125 strips) (Cat. No. Q33252)
• Qubit 1X dsDNA HS Assay Kit (500 assays) (Cat. No. Q33231)
• Qubit Flex Assay Reservoirs (100 reservoirs) (Cat. No. Q33253)
• WiFi Dongle (Cat. No. A26774)
Qubit Flex SmartStart Digital Educational Experience—includes interactive visual demonstrations of how to install, operate, and maintain your instrument
Three-year Extended Warranty—if your instrument needs repair we will send you a factory-certified refurbished replacement

The Qubit Flex Fluorometer is a benchtop fluorometer designed for highly accurate quantification of DNA, RNA, microRNA and protein. With the Qubit Flex Fluorometer you have the flexibility to directly measure fluorescence of up to eight samples simultaneously, which reduces assay variability. Paired with Qubit reagents, the Qubit Flex Fluorometer is part of an optimized workflow generating highly accurate and reproducible results. The fluorometer has a large screen and intuitive user interface making it easy to select and run your desired assay with results displayed in just a few seconds.

For all Qubit assays, the concentration of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants on the result. The Qubit 1X dsDNA HS Assay Kit is included in this NGS starter kit. Simply combine the reagent and the buffer, creating a ready-to-use formulation that is stable for up to one year after preparation.

Qubit™ RNA IQ Standards Invitrogen™

The Qubit RNA IQ (integrity & quality) Assay RNA standards are designed for use with the Qubit RNA IQ Assay Kit. Sufficient amounts of the small and large and/or structured RNA standard required for an IQ standard curve are supplied to perform standards with up to 500 samples. The RNA IQ standards are designed to measure RNA degradation and are not interchangeable with the standards from the RNA quantitation assays, including the Qubit RNA HS, BR, or XR assay kits.

Note: 500-mL thin-walled PCR tubes (Cat. No. Q32856) are required for all Qubit assays and are not included with these Qubit assay standards.

Qubit™ 4 NGS Starter Kit, with WiFi Invitrogen™

The Qubit 4 NGS Starter Kit for next-generation sequencing includes:
• One Qubit 4 Fluorometer (Cat. No. Q33226)
• Qubit Assay Tubes (set of 500) (Cat. No. Q32856)
• WiFi Dongle (Cat. No. A26774)
• Qubit 1X dsDNA HS Assay Kit, 500 assays

The Qubit 4 Fluorometer with WiFi is the latest version of the popular Qubit fluorometer designed to accurately measure DNA, RNA, and protein quantity, and now also RNA integrity and quality, using the highly sensitive Qubit assays. The Qubit 4 Fluorometer was re-engineered to enable data transfer via WiFi as well as to run the Qubit RNA IQ (integrity & quality) assay. The Qubit 4 Fluorometer and RNA IQ Assay Kit work together to accurately distinguish intact from degraded RNA in just two easy steps.

For all Qubit assays, the concentration or quality of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants on the result. The easy-to-use touch-screen menus make it easy to select and run the assays you need, with results displayed in just a few seconds.

Key features of the Qubit 4 Fluorometer include:
• Fast and highly accurate quantitation of DNA, RNA, and protein in less than three seconds per sample
• Measures intact RNA in less than 5 seconds per sample
• High levels of accuracy using only 1–20 μL of sample, even with very dilute samples
• On-board Reagent Calculator quickly generates Qubit working solution preparation instructions
• Stores results from up to 1000 samples
• Large 5.7-inch, state-of-the-art color touch screen for easy workflow navigation
• Graphical display indicates when samples are in the extended range or out of range
• Small footprint saves space on your bench
• Flexibility to export data to using the included WiFi dongle, a USB drive, or directly to your computer via a USB cable
• Ability to personalize your Qubit fluorometer with the assays you run most, add new assays, or even create your own assays with the MyQubit software and web tool
• Language of your choice including English, French, Spanish, Italian, German, simplified Chinese, and Japanese
• Pre-loaded program to test performance of fluorometer using the Qubit Fluorometer Verification Assay Kit

Learn more about the Qubit Fluorometer ›

The Qubit 4 Fluorometer can also be used to directly measure the fluorescence of samples using Fluorometer mode. Additionally, Ion Sphere Particle quality can be evaluated on the Qubit 4 Fluorometer using the Ion Sphere Quality Control Kit prior to performing a sequencing run on the Ion PGM sequencer.

Enables more accurate results than with UV absorbance at low concentrations
The Qubit 4 Fluorometer is far more sensitive than UV absorbance, which measures anything absorbing at 260 nm, including DNA, RNA, protein, free nucleotides, or excess salts. Moreover, UV spectrophotometry is often not sensitive enough to accurately measure low concentrations of DNA and RNA. With the Qubit fluorometer, your research is enhanced by more accurate measurements because the dyes in the Qubit assay kits fluoresce only when bound to the selected molecule (DNA, RNA or protein) in your sample. This allows you to avoid repeating work due to inaccurate measurements.

Fast, simple, and accurate measurement of RNA quality
The Qubit RNA IQ assay provides a fast, simple method to check whether an RNA sample has degraded. The assay utilizes two unique dyes: one that binds to large, intact RNA, and another that selectively binds to small, degraded RNA. Together they enable you to quickly assess the quality and integrity of an RNA sample. To use, simply add your samples to the RNA IQ working solution, then measure on the Qubit 4 Fluorometer.

Save samples and bench space
The thoughtfully designed Qubit 4 Fluorometer requires samples of only 1–20 µL. It is intended for use at room temperature and occupies only a small amount of bench space. The Qubit 4 Fluorometer features advanced optics and data analysis algorithms, and comes equipped with a WiFi dongle, a USB thumb drive, and cable for data transfer to Excel™ software and for software downloads, as well as a universal power supply, four plug adapters, and electromagnetic CE certification. The Qubit 4 Fluorometer enables maximum flexibility for data export using WiFi, USB drive, or USB cable direct to computer. Additionally, all data can be saved in the Qubit cloud application, including direct transfer from the instrument via WiFi.

Single sample measurements are a breeze
This user-friendly fluorometer is combined with quick and simple assay procedures. Calculations and settings are automatically performed by the instrument. The Qubit assays for use with the Qubit 4 Fluorometer are all performed using the same general protocol, which uses a simple mix-and-read format with incubation times of only two minutes for the DNA and RNA assays. 500 L thin-walled PCR tubes (Cat. No. Q32856) are required.

Qubit™ Flex Fluorometer Invitrogen™

The Qubit Flex Fluorometer is a benchtop fluorometer designed for highly accurate quantification of DNA, RNA, microRNA, and protein. With the Qubit Flex Fluorometer you have the flexibility to directly measure fluorescence of up to eight samples simultaneously, which reduces assay variability. Paired with Qubit reagents, the Qubit Flex Fluorometer is part of an optimized workflow generating highly accurate and reproducible results. The fluorometer has a large screen and intuitive user interface, making it easy to select and run your desired assay with results displayed in just a few seconds.

Features of the Qubit Flex Fluorometer include:
Improved throughput—measure multiple samples and quantify DNA, RNA, and protein up to 50% faster than single-sample readers
Flexibility—measure up to eight samples simultaneously
Complete optimized system—works seamlessly with Qubit reagents to generate reliable, sensitive, and specific results
Accurate quantification—high levels of accuracy using only 1–20 μL of sample, even with very dilute samples
On-board calculators—quickly generates Qubit working solution preparation instructions and determines the molarity of your samples
Easy to use—a large 8-inch, state-of-the-art color touchscreen and intuitive user interface results in easy workflow navigation
Data export flexibility—data is transferred easily using the included WiFi dongle, a USB drive, or directly to your computer
Several languages available—choices include English, French, Spanish, Italian, German, simplified Chinese, and Japanese
Verification test—a pre-loaded program is included to verify performance of the instrument
Qubit Flex SmartStart Digital Educational Experience—includes interactive visual demonstrations of how to install, operate, and maintain your instrument
Three-year Extended Warranty—if your instrument needs repair we will send you a factory-certified refurbished replacement

Higher throughput, less variability than single sample readers
The Qubit Flex Fluorometer has the flexibility to measure multiple DNA, RNA, and protein samples up to 50% faster than single-sample readers. In addition to saving time, the ability to measure eight samples simultaneously reduces variability, resulting in highly reproducible data. The Qubit Flex Fluorometer includes Reagent, Range, and Molarity calculators, further reducing the time to results. The Reagent and Range calculators help to determine which reagent and amount of sample to use to effectively quantify the amount of DNA, RNA, or protein. The Molarity calculator allows you to calculate the molarity of your samples based on nucleic acid length and the measured concentration.

Enables more accurate results than UV absorbance at low concentrations
The Qubit Flex Fluorometer is far more sensitive than UV absorbance, which measures anything absorbing at 260 nm, including DNA, RNA, protein, free nucleotides, and excess salts. Moreover, UV spectrophotometry is often not sensitive enough to accurately measure low concentrations of DNA and RNA. With the Qubit Flex fluorometer, your research is enhanced by more accurate measurements because the dyes in the Qubit assay kits fluoresce only when bound to the target molecule (DNA, RNA, or protein) in your sample. This allows you to avoid repeating work due to inaccurate measurements.

Optimized solution consisting of instrument and reagents results in highly reproducible data
The thoughtfully designed Qubit Flex Fluorometer requires sample volumes of only 1–20 µL. The advanced optics and data analysis algorithms were designed and optimized to work in perfect harmony with Qubit reagents, resulting in a seamless solution that generates highly reliable, sensitive, and specific results.

Qubit™ 1X dsDNA HS Assay Lambda Standard Invitrogen™

The Qubit dsDNA HS (High-Sensitivity) Assay Lambda Standard can be used with either the Qubit 1X dsDNA HS Assay Kit or the Qubit dsDNA HS Assay Kit. Sufficient material is supplied to perform standards with up to 500 samples.

Note: 500-mL thin-walled PCR tubes (Cat. No. Q32856) are required for all Qubit assays and are not included with this Qubit assay standard.
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