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SlowFade™ Diamond Antifade Mountant with DAPI Invitrogen™

SlowFade Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. It provides superior antifade protection across the entire visual and IR spectrums. You can use SlowFade Diamond mountant with almost any fluorescent dye or fluorescent protein (e.g., GFP, RFP, mCherry) to achieve the brightest signal and lowest background.

Features of SlowFade Diamond Antifade Mountant include:
• Protects fluorescent dyes and fluorescent protein from fading, across entire visible and IR spectrums
• Ready-to-use liquid that does not cure; samples can be viewed immediately
• Ideal for Alexa Fluor and traditional dyes, such as FITC and Cy3, and fluorescent proteins like GFP, RFP, and mCherry
• Maintains fluorescence signal—little to no quenching

Select the antifade mounting or optical clearing reagent that matches your fluorescence imaging needs ›

For high resolution or to image thicker tissue or 3D cell culture with a focal depth of 0–500 µm, try SlowFade Glass Antifade Mountant. It is a RIMS mountant with refractive index of 1.52.

Select the best SlowFade Antifade reagents for your experimental needs ›

SlowFade Diamond Antifade Mountant comes already mixed and ready to use in a dropper bottle, with or without DAPI. Just apply a drop to the sample, add a coverslip, and image. When the coverslip edges are sealed with an appropriate coverslip sealant, samples can be stored for days to weeks. If long term storage is desired with a refractive index similar to glass or immersion oil, then ProLong Glass Antifade Mountant is recommended. With the no-curing-required properties of SlowFade Diamond mountant, samples can be evaluated and imaged right after labeling.

Get photobleaching protection across the entire visible and IR spectrums
No matter which fluorescent dyes or fluorescent proteins you are using, SlowFade Diamond mountant helps provide the best protection against photobleaching. It is proven to have best-in-class performance with a wide array of fluorescent dyes, including Alexa Fluor dyes, FITC, TRTC, Texas Red, Cy3, and Cy5. SlowFade Diamond mountant does not quench, and its ability to provide brighter signal, against no background, helps ensure that you obtain the best-quality, high resolution images for publishing.

Stable signals during imaging and for immediate viewing
SlowFade Diamond mountant is the ideal option for analyzing samples right after labeling. Its antifade properties for both fluorescent dyes and fluorescent proteins preserves signal strength during prolonged imaging sessions, such as repeated scanning under confocal laser scanning illumination, or with super resolution microscopy. And it's easy to use—simply remove excess water from the stained sample and mount. A slide sealant can be used to preserve the slides for days or weeks. If longer archiving is desired, then the SlowFade Diamond mountant can be washed away with PBS, and the samples remounted with ProLong Diamond Antifade Mountant.

Achieve excellent refractive index for crisp images
For modern high-resolution imaging applications, refractive index (RI) matching is critical. SlowFade® Diamond mountant has a refractive index of 1.42 and is recommended for cultured cells or thin slices of tissue (less then 10 µm in thickness).

SlowFade™ Diamond Antifade Mountant Invitrogen™

SlowFade Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. It provides superior antifade protection across the entire visual and IR spectrums. You can use SlowFade Diamond mountant with almost any fluorescent dye or fluorescent protein (e.g., GFP, RFP, mCherry) to achieve the brightest signal and lowest background.

Features of SlowFade Diamond Antifade Mountant include:
• Protects fluorescent dyes and fluorescent protein from fading, across entire visible and IR spectrums
• Ready-to-use liquid that does not cure; samples can be viewed immediately
• Ideal for Alexa Fluor and traditional dyes, such as FITC and Cy3, and fluorescent proteins like GFP, RFP, and mCherry
• Maintains fluorescence signal—little to no quenching

Select the antifade mounting or optical clearing reagent that matches your fluorescence imaging needs ›

For high resolution or to image thicker tissue or 3D cell culture with a focal depth of 0–500 µm, try SlowFade Glass Antifade Mountant. It is a RIMS mountant with refractive index of 1.52.

Select the best SlowFade Antifade reagents for your experimental needs ›

SlowFade Diamond Antifade Mountant comes already mixed and ready to use in a dropper bottle, with or without DAPI. Just apply a drop to the sample, add a coverslip, and image. When the coverslip edges are sealed with an appropriate coverslip sealant, samples can be stored for days to weeks. If long term storage is desired with a refractive index similar to glass or immersion oil, then ProLong Glass Antifade Mountant is recommended. With the no-curing-required properties of SlowFade Diamond mountant, samples can be evaluated and imaged right after labeling.

Get photobleaching protection across the entire visible and IR spectrums
No matter which fluorescent dyes or fluorescent proteins you are using, SlowFade Diamond mountant helps provide the best protection against photobleaching. It is proven to have best-in-class performance with a wide array of fluorescent dyes, including Alexa Fluor dyes, FITC, TRTC, Texas Red, Cy3, and Cy5. SlowFade Diamond mountant does not quench, and its ability to provide brighter signal, against no background, helps ensure that you obtain the best-quality, high resolution images for publishing.

Stable signals during imaging and for immediate viewing
SlowFade Diamond mountant is the ideal option for analyzing samples right after labeling. Its antifade properties for both fluorescent dyes and fluorescent proteins preserves signal strength during prolonged imaging sessions, such as repeated scanning under confocal laser scanning illumination, or with super resolution microscopy. And it's easy to use—simply remove excess water from the stained sample and mount. A slide sealant can be used to preserve the slides for days or weeks. If longer archiving is desired, then the SlowFade Diamond mountant can be washed away with PBS, and the samples remounted with ProLong Diamond Antifade Mountant.

Achieve excellent refractive index for crisp images
For modern high-resolution imaging applications, refractive index (RI) matching is critical. SlowFade® Diamond mountant has a refractive index of 1.42 and is recommended for cultured cells or thin slices of tissue (less then 10 µm in thickness).

ProLong™ Diamond Antifade Mountant with DAPI Invitrogen™

ProLong Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. It is formulated without DNA stain or with the blue DNA stain DAPI or with the deep red DNA stain SYTOX Deep Red. ProLong Diamond mountant is designed to provide unparalleled antifade protection across the entire visual and IR spectrums. You can use ProLong Diamond mountant with almost any fluorescent dye or fluorescent protein (e.g., GFP, RFP, mCherry) to achieve the brightest signal and lowest background, for bright, high definition fluorescent images.

Features of ProLong Diamond Antifade Mountant include:
• Protects fluorescent dyes and fluorescent protein from fading, across entire visible and IR spectrums
• Ready-to-use liquid that cures for longer-term storage
• Ideal for Alexa Fluor and traditional dyes, such as FITC and Cy3, and fluorescent proteins like GFP, RFP, and mCherry
• Mounted samples are stable for months
• Maintains fluorescence signal—little to no quenching

Learn more about ProLong Antifade mountants.

For mountants with 1.52 refractive index, optimized for oil immersion objective imaging, try curing mountant Prolong Glass Antifade Mountant or non-curing SlowFade Glass Soft-set Antifade Mountant.

ProLong Diamond Antifade Mountant comes already mixed and ready to use in a dropper bottle or 10-mL bottle with or without DNA stain. DAPI stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm, and is detected using a DAPI traditional filter. SYTOX Deep Red stain is excited by red light at 660 nm when bound to DNA, with an emission maximum at 682 nm, and is detected using a Cy5/deep red traditional filter. Just apply a drop to the sample, add a coverslip, cure, and image. ProLong Diamond mountant is a curing mountant that forms a near-perfect optical path (1.47 RI) and enables longer-term storage of your samples, while protecting them from photobleaching. ProLong Diamond mountant does not discolor or shrink when cured and stored long term, making it possible to take high quality fluorescent images weeks or even months after mounting the slides.

Get Photobleaching Protection Across the Entire Visible and IR Spectrums
No matter which fluorescent dyes or fluorescent proteins you are using, ProLong Diamond mountant helps provide superior protection against photobleaching. It is proven to have best-in-class performance with a wide array of fluorescent dyes, including Alexa Fluor dyes, FITC, TRTC, Texas Red, Cy3, and Cy5. ProLong Diamond mountant does not quench fluorophores, and its ability to provide brighter signal, against no background, helps ensure that you get the best-quality, high resolution images for publishing.

Stable Signals During Imaging and for Longer-term Storage
ProLong Diamond mountant is the ideal option for archiving samples with fluorescent dyes or fluorescent proteins. It is also an ideal option for preserving signal strength during prolonged imaging sessions, such as repeated scanning under confocal laser scanning illumination, or with super resolution microscopy. And it's easy to use—simply remove excess water from the stained sample and mount. For immediate viewing of the sample, choose our non-curing mountant, SlowFade Diamond Antifade Mountant.

Achieve Excellent Refractive Index Matching for Crisp Images
For modern high-resolution imaging applications, refractive index (RI) matching is critical. ProLong Diamond mountant will cure to an RI of 1.47. Additionally, this curing property means you do not need to use messy acrylic resins (i.e., finger nail polish), which can damage or ruin modern microscope objectives if it comes into contact with them.

SlowFade™ Glass Soft-set Antifade Mountant, with DAPI Invitrogen™

SlowFade Glass Antifade Mountant is a glycerol-based, ready-to-use, non-curing mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It is formulated to have a refractive index of 1.52 for sharper images and antifade components to resist photobleaching. The result is sharp, bright images with almost any fluorophore, with wide-field, confocal, or super-resolution microscopes, at focal depths of 0–500 µm.

Since SlowFade Glass Antifade Mountant is non-curing, cells can be imaged immediately after mounting, without any of the adverse effects on cell morphology that a curing mountant might cause. It comes ready to use—just apply a drop to the sample, add a coverslip, and image. It is available with or without DAPI nuclear stain.

Features of SlowFade Glass Antifade Mountant include:
• Ready-to-use, glycerol-based, non-curing antifade mountant
• Refractive index of 1.52, with minimized spherical aberrations
• Up to three times better axial resolution compared to regular mountant
• Visible tissue optical clearing, even in sections up to 1-mm thick
• Sharp, bright images at any focal depth from 0 to 500 µm

Sharp images for deep 3D imaging
SlowFade Glass Antifade Mountant features a refractive index of 1.52 (similar to glass coverslips), with superior photobleach protection. It is compatible with immersion oil and oil-immersion microscope optics, enabling a refraction-free light path and minimized spherical aberration, which improves axial resolution by three times (at 150-µm focal depth) as measured by point spread function. When 1-mm thick tissue sections are mounted, there is visible optical tissue clearing, enabling deep 3D imaging.

Inhibit photobleaching across the visible and near-IR spectra
No matter which fluorescent dyes or fluorescent proteins you are using, Slowfade Glass Antifade Mountant helps provide superior protection against photobleaching. It has been shown to have best-in-class performance with a wide array of fluorescent dyes, including Alexa Fluor, FITC, TRTC, Texas Red, Cy3, and Cy5 dyes, and fluorescent proteins, including GFP, RFP, and mCherry. SlowFade Glass mountant does not quench fluorophores, and its ability to provide brighter signal against no background helps ensure that you get the best-quality high-resolution images for publishing.

Non-curing mountant which does not expand or shrink specimens
Most tissue clearing techniques either expand or shrink the specimens, causing artifacts in specimen morphology. SlowFade Glass Antifade Mountant is non-curing and does not cause expansion or contraction of specimens, which we have verified using Hela spheroids and rat brain sections. This characteristic allows 3D imaging of truly native specimen morphology. The specimens are available for imaging immediately after mounting, and properly sealed slides can be stored for weeks or months for excellent

ProLong™ Gold Antifade Mountant with SYTOX™ Deep Red Invitrogen™

ProLong Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It is formulated without DNA stain or with the blue DNA stain DAPI or with the deep red DNA stain SYTOX Deep Red. ProLong Gold mountant contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments, and delivers this protection without significantly quenching the initial fluorescence signal. It is a curing mountant that forms a near-perfect optical path (1.47 RI) and allows longer-term storage of the sample. It comes ready-to-use (room temperatures storage and no mixing required)—just apply a drop to the sample, add a coverslip, cure, and image.

ProLong Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins, such as GFP. For superior antifade protection of fluorescent proteins and fluorescent dyes, ProLong Diamond Antifade Mountant is recommended. Learn more about key differences between ProLong Gold and ProLong Diamond antifade mountants.

Key Attributes:

• Protects dyes from fading during imaging
• Ready-to-use liquid that cures for longer-term storage
• Mounted samples are stable for months
• Maintains fluorescence signal—little to no quenching
• Ideal for Alexa Fluor dyes

For mountants with 1.52 refractive index, optimized for oil immersion objective imaging, try curing mountant Prolong Glass Antifade Mountant or non-curing SlowFade Glass Soft-set Antifade Mountant.

ProLong Gold Antifade Mountant comes already mixed and ready to use in a dropper bottle or 10-mL bottle with or without DNA stain. DAPI stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm, and is detected using a DAPI traditional filter. SYTOX Deep Red stain is excited by red light at 660 nm when bound to DNA, with an emission maximum at 682 nm, and is detected using a Cy5/deep red traditional filter.

Get Photobleaching Protection Without Quenching
Many mountants can destroy initial fluorescence signal strength by up to 80%. To create the Molecular Probes Gold series of mountants, our scientists developed a formula of chemical additives that minimize this initial signal quenching for the broadest range of colors and dyes.

Stable Signals During Imaging and for Longer-Term Storage
While epoxy-based mountants used in our FluoCells series of prepared cells can be stable for years and afford the highest level of antifade protection, they are difficult to use, requiring a series of steps in organic solvents prior to mounting. ProLong Gold Antifade Mountant is the next-best option for archiving samples. And it is the best option for preserving signal strength during prolonged imaging sessions, such as repeated scanning under confocal laser scanning illumination. It's easy to use—just air-dry your stained samples to remove excess water and mount. For immediate viewing of the sample, choose our non-curing mountant, SlowFade Gold Antifade Mountant.

Achieve Excellent Refractive Index Matching Without Acrylic Resins
For modern high-resolution imaging applications, refractive index matching is critical. ProLong Gold Antifade Mountant will cure to an Rf of 1.47. Additionally, the curing property means you can avoid messy acrylic resins (i.e., finger nail polish), which can damage or ruin modern microscope objectives if it comes into contact with them.

SlowFade™ Glass Soft-set Antifade Mountant Invitrogen™

SlowFade Glass Antifade Mountant is a glycerol-based, ready-to-use, non-curing mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It is formulated to have a refractive index of 1.52 for sharper images and antifade components to resist photobleaching. The result is sharp, bright images with almost any fluorophore, with wide-field, confocal, or super-resolution microscopes, at focal depths of 0–500 µm.

Since SlowFade Glass Antifade Mountant is non-curing, cells can be imaged immediately after mounting, without any of the adverse effects on cell morphology that a curing mountant might cause. It comes ready to use—just apply a drop to the sample, add a coverslip, and image. It is available with or without DAPI nuclear stain.

If a curing, hard-set mountant is needed, also with a refractive index of 1.52, for years of slide storage, then Prolong Glass Antifade Mountant is recommended.

Features of SlowFade Glass Antifade Mountant include:
• Ready-to-use, glycerol-based, non-curing antifade mountant
• Refractive index of 1.52, with minimized spherical aberrations
• Up to three times better axial resolution compared to regular mountant
• Visible tissue optical clearing, even in sections up to 1-mm thick
• Sharp, bright images at any focal depth from 0 to 500 µm

Sharp images for deep 3D imaging
SlowFade Glass Antifade Mountant features a refractive index of 1.52 (similar to glass coverslips), with superior photobleach protection. It is compatible with immersion oil and oil-immersion microscope optics, enabling a refraction-free light path and minimized spherical aberration, which improves axial resolution by three times (at 150-µm focal depth) as measured by point spread function. When 1-mm thick tissue sections are mounted, there is visible optical tissue clearing, enabling deep 3D imaging. For 3D imaging of whole organs (up to 10 mm), please see Clearing Reagents for Imaging 3D Cell Culture and Tissue.

Inhibit photobleaching across the visible and near-IR spectra
No matter which fluorescent dyes or fluorescent proteins you are using, Slowfade Glass Antifade Mountant helps provide superior protection against photobleaching. It has been shown to have best-in-class performance with a wide array of fluorescent dyes, including Alexa Fluor, FITC, TRTC, Texas Red, Cy3, and Cy5 dyes, and fluorescent proteins, including GFP, RFP, and mCherry. SlowFade Glass mountant does not quench fluorophores, and its ability to provide brighter signal against no background helps ensure that you get the best-quality high-resolution images for publishing.

Non-curing mountant which does not expand or shrink specimens
Most tissue clearing techniques either expand or shrink the specimens, causing artifacts in specimen morphology. SlowFade Glass Antifade Mountant is non-curing and does not cause expansion or contraction of specimens, which we have verified using Hela spheroids and rat brain sections. This characteristic allows 3D imaging of truly native specimen morphology. The specimens are available for imaging immediately after mounting, and properly sealed slides can be stored for weeks or months for excellent

ProLong™ Glass Antifade Mountant with SYTOX™ Deep Red Invitrogen™

ProLong Glass Antifade Mountant is a glycerol-based, hard-setting, ready-to-use mountant that can be applied directly to fluorescently labeled cells or tissue samples on microscope slides or coverslips. It is formulated without DNA stain or with the blue DNA stain NucBlue (Hoechst 33342) or with the deep red DNA stain SYTOX Deep Red. Prolong Glass mountant has a refractive index of 1.52 after curing, similar to that of glass coverslips, compatible immersion oil, and oil-immersion microscope optics, enabling superior resolution and sensitivity. ProLong Glass mountant is also designed to provide unparalleled photobleach protection across the visible and near infra-red spectra. You can use ProLong Glass Antifade Mountant with almost any fluorescent dye or fluorescent protein (e.g., GFP, RFP, mCherry) and with any cell/tissue type for image depths ranging from 0 to 100 µm for bright, high-resolution Z-Stack, 3D, and 2D images. For a non-curing mountant with 1.52 refractive index, try SlowFade Glass Soft-set Antifade Mountant.

With its high refractive index of 1.52 and superior antifade formulation, ProLong Glass Antifade Mountant delivers the following benefits:
• Up to 75% improvement in axial resolution for sharp images
• 3–4 times more imageable focal depth compared tomountants with refractive index of 1.47, with sharp images for monolayers of cells, tissue slices, or 3D cell cultures up to 150 μm thickness/focal depth
• Low background across the spectrum
• Preserved signals from inhibited photobleaching
• Weeks to months of stability for slide-mounted samples
• Ease of use
• Availability with or without DAPI

ProLong Glass Hard-set Antifade Mountant comes already mixed and ready to use in a dropper bottle or 10-mL bottle with or without DNA stain. NucBlue (Hoechst 33342) is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm, and is detected using a DAPI traditional filter. SYTOX Deep Red stain is excited by red light at 660 nm when bound to DNA, with an emission maximum at 682 nm, and is detected using a Cy5/deep red traditional filter. After removing excess water, just apply a drop of mountant to the sample, add a coverslip, cure, and image. ProLong Glass mountant is a hard-setting, curing mountant that minimizes optical refraction and spherical aberration in the optical path, enables longer-term storage of your samples, and helps protect your samples from photobleaching. ProLong Glass mountant does not discolor or shrink when cured, making it possible to take high-quality fluorescent images weeks, or even months, after mounting the slides.

ProLong Glass Antifade Mountant with NucBlue stain contains an optimized concentration of Hoechst 33342 stain, which provides superior nuclear labeling of most cells and tissues. ProLong Glass mountant with NucBlue stain is not recommended for specimens thicker than 30 µm. Thicker specimens can require longer incubations with labeling reagents, so a pre-incubation with NucBlue Live ReadyProbes Reagent is recommended.

Brightest and sharpest images
Studies show that the axial resolution of a microscopy system can be improved by matching the refractive index of the mounting medium, which maximizes light collection of the objective lens (1–3). Fouquet et. al. saw dramatic improvement in axial resolution while using a confocal microscope with high refractive index media (3). When Prolong Glass Antifade Mountant, which has a refractive index of 1.52, is com

ProLong™ Glass Antifade Mountant Invitrogen™

ProLong Glass Antifade Mountant is a glycerol-based, hard-setting, ready-to-use mountant that can be applied directly to fluorescently labeled cells or tissue samples on microscope slides or coverslips. It is formulated without DNA stain or with the blue DNA stain NucBlue (Hoechst 33342) or with the deep red DNA stain SYTOX Deep Red. Prolong Glass mountant has a refractive index of 1.52 after curing, similar to that of glass coverslips, compatible immersion oil, and oil-immersion microscope optics, enabling superior resolution and sensitivity. ProLong Glass mountant is also designed to provide unparalleled photobleach protection across the visible and near infra-red spectra. You can use ProLong Glass Antifade Mountant with almost any fluorescent dye or fluorescent protein (e.g., GFP, RFP, mCherry) and with any cell/tissue type for image depths ranging from 0 to 100 µm for bright, high-resolution Z-Stack, 3D, and 2D images. For a non-curing mountant with 1.52 refractive index, try SlowFade Glass Soft-set Antifade Mountant.

With its high refractive index of 1.52 and superior antifade formulation, ProLong Glass Antifade Mountant delivers the following benefits:
• Up to 75% improvement in axial resolution for sharp images
• 3–4 times more imageable focal depth compared tomountants with refractive index of 1.47, with sharp images for monolayers of cells, tissue slices, or 3D cell cultures up to 150 μm thickness/focal depth
• Low background across the spectrum
• Preserved signals from inhibited photobleaching
• Weeks to months of stability for slide-mounted samples
• Ease of use
• Availability with or without DAPI

Learn more about ProLong Antifade mountants >

ProLong Glass Hard-set Antifade Mountant comes already mixed and ready to use in a dropper bottle or 10-mL bottle with or without DNA stain. NucBlue (Hoechst 33342) is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm, and is detected using a DAPI traditional filter. SYTOX Deep Red stain is excited by red light at 660 nm when bound to DNA, with an emission maximum at 682 nm, and is detected using a Cy5/deep red traditional filter. After removing excess water, just apply a drop of mountant to the sample, add a coverslip, cure, and image. ProLong Glass mountant is a hard-setting, curing mountant that minimizes optical refraction and spherical aberration in the optical path, enables longer-term storage of your samples, and helps protect your samples from photobleaching. ProLong Glass mountant does not discolor or shrink when cured, making it possible to take high-quality fluorescent images weeks, or even months, after mounting the slides.

ProLong Glass Antifade Mountant with NucBlue stain contains an optimized concentration of Hoechst 33342 stain, which provides superior nuclear labeling of most cells and tissues. ProLong Glass mountant with NucBlue stain is not recommended for specimens thicker than 30 µm. Thicker specimens can require longer incubations with labeling reagents, so a pre-incubation with NucBlue Live ReadyProbes Reagent is recomended.

Brightest and sharpest images
Studies show that the axial resolution of a microscopy system can be improved by matching the refractive index of the mounting medium, which maximizes light collection of the objective lens (1–3). Fouquet et. al. saw dramatic improvement in axial resolution while using a confocal microscope with high refractive index media (3). When Prolong Glass Antifade Mountant, which has a refractive index of 1.52, is compared with a mounting medium with a refractive index of 1.47, the axial resolution is improved by 32% at a focal depth of 40 µm and 75% at a focal depth of 100 µm (see figure below). The signal intensity and sensitivity of fluorophores is also improved as the focal depth increases within the sample. The same or greater improvement in resolution and sensitivity is seen in the lateral direction. This makes Prolong Glass a superior mountant for acquisition of Z-Stack, 3D, and 2D images of cells and tissue at depths from 0 to 100 µm (see figure below).

Inhibit photobleaching across the visible and near-IR spectra
No matter which fluorescent dyes or fluorescent proteins you are using, ProLong Glass Antifade Mountant helps provide superior protection against photobleaching. It has been shown to have best-in-class performance with a wide array of fluorescent dyes, including Alexa Fluor, FITC, TRTC, Texas Red, Cy3, and Cy5 dyes (see figure below). ProLong Glass mountant does not quench fluorophores, and its ability to provide brighter signal against no background helps ensure that you get the best-quality high-resolution images for publishing.

Stable signals during imaging and for longer-term storage
ProLong Glass Antifade Mountant is the ideal option for archiving samples with fluorescent dyes or fluorescent proteins and for preserving signal strength during prolonged imaging sessions, such as repeated scanning under confocal laser scanning illumination or with super-resolution microscopy. And it's easy to use—simply remove excess water from the stained sample and mount.

Achieve excellent refractive index matching for crisp images
For modern high-resolution imaging applications, refractive index matching is critical. ProLong Glass Antifade Mountant cures to a refractive index of 1.52. Additionally, this curing property eliminates the need to use messy acrylic resins (i.e., fingernail polish), which can damage or ruin modern microscope objectives.

References
1: Cremer C, Masters BR. Resolution enhancement techniques in microscopy. Eur Phys J. 2013 38(3):281-344.
2: Shimi T, Kittisopikul M, Tran J, Goldman AE, Adam SA, Zheng Y, Jaqaman K, Goldman RD. Structural organization of nuclear lamins A, C, B1, and B2 revealed by superresolution microscopy. Mol Biol Cell. 2015 26(22):4075-86.
3: Fouquet C, Gilles JF, Heck N, Dos Santos M, Schwartzmann R, Cannaya V, Morel MP, Davidson RS, Trembleau A, Bolte S. Improving axial resolution in confocal microscopy with new high refractive index mounting media. PLoS One. 2015 10(3):e0121096.

Fluoromount-G™ Mounting Medium Invitrogen™

Fluoromount-G Mounting Medium is a clear liquid medium designed for use in mounting slides following immunofluorescent staining. For convenience, this medium is provided with and without DAPI, a blue nuclear counter stain. It is water soluble and used to mount slides in which the final step of staining is aqueous. It forms a semi-permanent seal for prolonged storage of slides at 2-8°C. Fluoromount-G Mounting Medium does not fluoresce and may reduce the amount of fluorochrome quenching and photobleaching during fluorescence microscopy. This medium is supplied as a 1X stock solution containing sodium azide. The refractive index of Fluoromount-G Mounting Medium is reported to be ~1.4, and it is best used when the thickness of the specimen is less than 10 µm.

Select the antifade mounting or optical clearing reagent that matches your fluorescence imaging needs ›

For mountants with a 1.52 refractive index, optimized for oil immersion objective imaging, try curing mountant Prolong Glass Antifade Mountant or non-curing SlowFade Glass Soft-set Antifade Mountant.

ProLong™ Diamond Antifade Mountant Invitrogen™

ProLong Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. It is formulated without DNA stain or with the blue DNA stain DAPI or with the deep red DNA stain SYTOX Deep Red. ProLong Diamond mountant is designed to provide unparalleled antifade protection across the entire visual and IR spectrums. You can use ProLong Diamond mountant with almost any fluorescent dye or fluorescent protein (e.g., GFP, RFP, mCherry) to achieve the brightest signal and lowest background, for bright, high definition fluorescent images.

Features of ProLong Diamond Antifade Mountant include:
• Protects fluorescent dyes and fluorescent protein from fading, across entire visible and IR spectrums
• Ready-to-use liquid that cures for longer-term storage
• Ideal for Alexa Fluor and traditional dyes, such as FITC and Cy3, and fluorescent proteins like GFP, RFP, and mCherry
• Mounted samples are stable for months
• Maintains fluorescence signal—little to no quenching

Learn more about ProLong Antifade mountants.

For mountants with 1.52 refractive index, optimized for oil immersion objective imaging, try curing mountant Prolong Glass Antifade Mountant or non-curing SlowFade Glass Soft-set Antifade Mountant.

ProLong Diamond Antifade Mountant comes already mixed and ready to use in a dropper bottle or 10-mL bottle with or without DNA stain. DAPI stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm, and is detected using a DAPI traditional filter. SYTOX Deep Red stain is excited by red light at 660 nm when bound to DNA, with an emission maximum at 682 nm, and is detected using a Cy5/deep red traditional filter. Just apply a drop to the sample, add a coverslip, cure, and image. ProLong Diamond mountant is a curing mountant that forms a near-perfect optical path (1.47 RI) and enables longer-term storage of your samples, while protecting them from photobleaching. ProLong Diamond mountant does not discolor or shrink when cured and stored long term, making it possible to take high quality fluorescent images weeks or even months after mounting the slides.

Get Photobleaching Protection Across the Entire Visible and IR Spectrums
No matter which fluorescent dyes or fluorescent proteins you are using, ProLong Diamond mountant helps provide superior protection against photobleaching. It is proven to have best-in-class performance with a wide array of fluorescent dyes, including Alexa Fluor dyes, FITC, TRTC, Texas Red, Cy3, and Cy5. ProLong Diamond mountant does not quench fluorophores, and its ability to provide brighter signal, against no background, helps ensure that you get the best-quality, high resolution images for publishing.

Stable Signals During Imaging and for Longer-term Storage
ProLong Diamond mountant is the ideal option for archiving samples with fluorescent dyes or fluorescent proteins. It is also an ideal option for preserving signal strength during prolonged imaging sessions, such as repeated scanning under confocal laser scanning illumination, or with super resolution microscopy. And it's easy to use—simply remove excess water from the stained sample and mount. For immediate viewing of the sample, choose our non-curing mountant, SlowFade Diamond Antifade Mountant.

Achieve Excellent Refractive Index Matching for Crisp Images
For modern high-resolution imaging applications, refractive index (RI) matching is critical. ProLong Diamond mountant will cure to an RI of 1.47. Additionally, this curing property means you do not need to use messy acrylic resins (i.e., finger nail polish), which can damage or ruin modern microscope objectives if it comes into contact with them.

ProLong™ Gold Antifade Mountant with DAPI

ProLong Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It is formulated without DNA stain or with the blue DNA stain DAPI or with the deep red DNA stain SYTOX Deep Red. ProLong Gold mountant contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments, and delivers this protection without significantly quenching the initial fluorescence signal. It is a curing mountant that forms a near-perfect optical path (1.47 RI) and allows longer-term storage of the sample. It comes ready-to-use (room temperatures storage and no mixing required)—just apply a drop to the sample, add a coverslip, cure, and image.

ProLong Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins, such as GFP. For superior antifade protection of fluorescent proteins and fluorescent dyes, ProLong Diamond Antifade Mountant is recommended. Learn more about key differences between ProLong Gold and ProLong Diamond antifade mountants.

Key Attributes:

• Protects dyes from fading during imaging
• Ready-to-use liquid that cures for longer-term storage
• Mounted samples are stable for months
• Maintains fluorescence signal—little to no quenching
• Ideal for Alexa Fluor dyes

For mountants with 1.52 refractive index, optimized for oil immersion objective imaging, try curing mountant Prolong Glass Antifade Mountant or non-curing SlowFade Glass Soft-set Antifade Mountant.

ProLong Gold Antifade Mountant comes already mixed and ready to use in a dropper bottle or 10-mL bottle with or without DNA stain. DAPI stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm, and is detected using a DAPI traditional filter. SYTOX Deep Red stain is excited by red light at 660 nm when bound to DNA, with an emission maximum at 682 nm, and is detected using a Cy5/deep red traditional filter.

Get Photobleaching Protection Without Quenching
Many mountants can destroy initial fluorescence signal strength by up to 80%. To create the Molecular Probes Gold series of mountants, our scientists developed a formula of chemical additives that minimize this initial signal quenching for the broadest range of colors and dyes.

Stable Signals During Imaging and for Longer-Term Storage
While epoxy-based mountants used in our FluoCells series of prepared cells can be stable for years and afford the highest level of antifade protection, they are difficult to use, requiring a series of steps in organic solvents prior to mounting. ProLong Gold Antifade Mountant is the next-best option for archiving samples. And it is the best option for preserving signal strength during prolonged imaging sessions, such as repeated scanning under confocal laser scanning illumination. It's easy to use—just air-dry your stained samples to remove excess water and mount. For immediate viewing of the sample, choose our non-curing mountant, SlowFade Gold Antifade Mountant.

Achieve Excellent Refractive Index Matching Without Acrylic Resins
For modern high-resolution imaging applications, refractive index matching is critical. ProLong Gold Antifade Mountant will cure to an Rf of 1.47. Additionally, the curing property means you can av

ProLong™ Diamond Antifade Mountant with SYTOX™ Deep Red Invitrogen™

ProLong Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. It is formulated without DNA stain or with the blue DNA stain DAPI or with the deep red DNA stain SYTOX Deep Red. ProLong Diamond mountant is designed to provide unparalleled antifade protection across the entire visual and IR spectrums. You can use ProLong Diamond mountant with almost any fluorescent dye or fluorescent protein (e.g., GFP, RFP, mCherry) to achieve the brightest signal and lowest background, for bright, high definition fluorescent images.

Features of ProLong Diamond Antifade Mountant include:
• Protects fluorescent dyes and fluorescent protein from fading, across entire visible and IR spectrums
• Ready-to-use liquid that cures for longer-term storage
• Ideal for Alexa Fluor and traditional dyes, such as FITC and Cy3, and fluorescent proteins like GFP, RFP, and mCherry
• Mounted samples are stable for months
• Maintains fluorescence signal—little to no quenching

Learn more about ProLong Antifade mountants.

For mountants with 1.52 refractive index, optimized for oil immersion objective imaging, try curing mountant Prolong Glass Antifade Mountant or non-curing SlowFade Glass Soft-set Antifade Mountant.

ProLong Diamond Antifade Mountant comes already mixed and ready to use in a dropper bottle or 10-mL bottle with or without DNA stain. DAPI stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm, and is detected using a DAPI traditional filter. SYTOX Deep Red stain is excited by red light at 660 nm when bound to DNA, with an emission maximum at 682 nm, and is detected using a Cy5/deep red traditional filter. Just apply a drop to the sample, add a coverslip, cure, and image. ProLong Diamond mountant is a curing mountant that forms a near-perfect optical path (1.47 RI) and enables longer-term storage of your samples, while protecting them from photobleaching. ProLong Diamond mountant does not discolor or shrink when cured and stored long term, making it possible to take high quality fluorescent images weeks or even months after mounting the slides.

Get Photobleaching Protection Across the Entire Visible and IR Spectrums
No matter which fluorescent dyes or fluorescent proteins you are using, ProLong Diamond mountant helps provide superior protection against photobleaching. It is proven to have best-in-class performance with a wide array of fluorescent dyes, including Alexa Fluor dyes, FITC, TRTC, Texas Red, Cy3, and Cy5. ProLong Diamond mountant does not quench fluorophores, and its ability to provide brighter signal, against no background, helps ensure that you get the best-quality, high resolution images for publishing.

Stable Signals During Imaging and for Longer-term Storage
ProLong Diamond mountant is the ideal option for archiving samples with fluorescent dyes or fluorescent proteins. It is also an ideal option for preserving signal strength during prolonged imaging sessions, such as repeated scanning under confocal laser scanning illumination, or with super resolution microscopy. And it's easy to use—simply remove excess water from the stained sample and mount. For immediate viewing of the sample, choose our non-curing mountant, SlowFade Diamond Antifade Mountant.

Achieve Excellent Refractive Index Matching for Crisp Images
For modern high-resolution imaging applications, refractive index (RI) matching is critical. ProLong Diamond mountant will cure to an RI of 1.47. Additionally, this curing property means you do not need to use messy acrylic resins (i.e., finger nail polish), which can damage or ruin modern microscope objectives if it comes into contact with them.

Fluoromount-G™ Mounting Medium, with DAPI Invitrogen™

Fluoromount-G Mounting Medium is a clear liquid medium designed for use in mounting slides following immunofluorescent staining. For convenience, this medium is provided with and without DAPI, a blue nuclear counter stain. It is water soluble and used to mount slides in which the final step of staining is aqueous. It forms a semi-permanent seal for prolonged storage of slides at 2-8°C. Fluoromount-G Mounting Medium does not fluoresce and may reduce the amount of fluorochrome quenching and photobleaching during fluorescence microscopy. This medium is supplied as a 1X stock solution containing sodium azide. The refractive index of Fluoromount-G Mounting Medium is reported to be ~1.4, and it is best used when the thickness of the specimen is less than 10 µm.

Select the antifade mounting or optical clearing reagent that matches your fluorescence imaging needs ›

For mountants with a 1.52 refractive index, optimized for oil immersion objective imaging, try curing mountant Prolong Glass Antifade Mountant or non-curing SlowFade Glass Soft-set Antifade Mountant.

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