Oligonucleotides

Oligonucleotides are short RNA or DNA oligomers with a wide range of applications in microarrays, gel shift assays, artificial gene synthesis, PCR, DNA sequencing, molecular cloning, forensics, and other research applications. Oligos are available in various primer lengths and may be pre-designed, customized, or randomized.
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Custom Primer Invitrogen™
Use this tool to design a custom DNA oligonucleotide, with any of a variety of 3' and 5' modifications, at scales ranging from 25 nmole to 10 μmole. Select desired purification method (normal or reversed-phase chromatography, HPLC, or PAGE).
Oligo(dT)20 Primer Invitrogen™
Used for first-strand cDNA synthesis with reverse transcriptase at temperatures of ≥50°C, Oligo(dT)20 Primer is a string of 20 deoxythymidylic acid residues that hybridizes to the poly(A) tail of mRNA.
Oligo(dT)18 Primer Thermo Scientific™
Thermo Scientific Oligo(dT)18 Primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends. The primer is supplied as a ready-to-use, 20X concentrated aqueous solution. Applications • cDNA Synthesis • RT-qPCR • RT-PCR Related products Anchored Oligo dT Random Hexamer...
Random Hexamer Primer Thermo Scientific™
Thermo Scientific Random Hexamer Primer is a mixture of single-stranded random hexanucleotides with 5'- and 3'-hydroxyl ends. The primer is supplied as a ready-to-use, 20X concentrated aqueous solution (24 μg at 100 μM).
Invitrogen Oligo(dT)16 Primer is a homo-oligomeric deoxyribonucleotide poly(dT) that is non-phosphorylated and chemically synthesized. It is used for priming and reverse transcription of polyadenylated poly(A)+ mRNA. Five nmoles of Oligo (dT)16 Primer is supplied in 100 μL of 10 mM TE, pH 7.
Random Hexamers are short oligodeoxyribonucleotides of random sequence [d(N)6] that anneal to random complementary sites on a target DNA or RNA to serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase.
Random Primers Invitrogen™
Random Primers are oligodeoxyribonucleotides (mostly hexamers) used to prepare labeled DNA probes from templates for filter hybridization or in situ hybridization and to prime mRNAs with or without poly(A) for cDNA synthesis.
Bio-16-UTP (10 mM) Invitrogen™
Ambion Bio-16-UTP 10 mM (Biotinylated UTP) is intended for use as substrate in in vitro transcription reactions. These modified nucleotides can be utilized by a variety of RNA polymerases, including T7, T3, and SP6 RNA polymerases, as well as other RNA modifying enzymes.
Oligo(dT)12-18 Primer is suitable for use in first-strand cDNA synthesis with reverse transcriptase. The primer hybridizes to the poly(A) tail of mRNA. It is phosphorylated on the 5´ end to facilitate cloning of cDNA.
This set of oligonucleotides is designed for template switching reverse transcription reaction and following preamplification of obtained cDNA. Template Switching Oligonucleotide (TSO) and Capturing Oligo (dT) are used during cDNA synthesis reaction to incorporate adaptor sequences in both ends of...
Exo-Resistant Random Primer Thermo Scientific™
Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions. This primer has two 3'-terminal phosphorothioate (PTO) modifications that are resistant to the 3'→5' exonuclease...
Random Decamers are the same primers currently included in the RETROscript™ Kit (Cat. No. AM1710). They are provided at a stock concentration of 50 μM, and are functionally tested using the RETROscript™ Kit.
These are the same Ambion® primers that are currently included in the RETROscript® Kit (SKU# AM1710). They are provided at a stock concentration of 50 μM and are functionally tested using the RETROscript® Kit.
Bio-11-UTP (10 mM) Invitrogen™
Ambion Bio-11-UTP 10 mM (Biotinylated UTP) is intended for use as substrate in in vitro transcription reactions. These modified nucleotides can be utilized by a variety of RNA polymerases, including T7, T3, and SP6 RNA polymerases, as well as other RNA modifying enzymes.
Ambion® Lambda DNA is digested to completion with Hind III. This generates fragments of the sizes 23130, 9416, 6557, 4361, 2322, 2027, 564, and 125 bp. These DNA molecular mass markers are supplied with a protocol describing easy labeling of the markers with radioisotopes.
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For easy ordering, check out out the Invitrogen Custom DNA oligos page., Invitrogen Custom DNA oligos are synthetic oligonucleotides made according to your specifications and can be used in a variety of applications, from PCR and sequencing to probes for gene detection.
Review selected protocols that are commonly used to spectrophotometrically quantify the concentration of an oligonucleotide or primer solution: how to concentrate or precipitate an oligo, how to PAGE purify oligos, and how to build adaptors with your oligos.
Documents & Support (4,676)
RISH Rembrandt Biotin and Digoxigenin-Labeled RNA Probes
What size of amine-modified oligonucleotides can be used with Alexa Fluor Oligonucleotide Amine Labeling Kits?