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7500 Fast Real-Time PCR Systems Spectral Calibration Kit II Applied Biosystems™

This easy-to-use kit establishes the pure dye spectra and multicomponent values for Cy3®, Cy5®, and Texas Red® dyes on the 7500 Fast Real-Time PCR System. The Applied Biosystems® 7500 Real-Time PCR Systems Spectral Calibration Kit II contains three Optical 96-Well Fast Thermocycling Plates preloaded and sealed with Cy3®, Cy5®, and Texas Red® fluorescent dye standards.

• 96-well optical reaction plates preloaded with Cy3®, Cy5®, and Texas Red® dyes allow quick and easy calibration of the 7500 Fast Real-Time PCR System
• Easy-to-use, preloaded spectral calibration kit allows accurate discrimination of the individual dyes in a reaction mixture when all the dyes are excited simultaneously

Spend Your Time on Research, Not Instrument Calibration
This kit's easy-to-use, preloaded, 96-well optical reaction plates enable quick and easy calibration of the 7500 Fast Real-Time PCR System. Insert the reaction plates during system installation, and the system collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

AccuSEQ™ Real-Time PCR Detection Software, version 3.0, seat license Applied Biosystems™

This is a seat license for AccuSEQ Real-Time PCR Detection Software v3.0 (Cat. No. A40303). Seat licenses are purchased for each individual user of the software.

AccuSEQ Real-Time PCR Detection Software, version 3.0, is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing using the SEQ family of real-time PCR assays, including MycoSEQ, resDNASEQ, and ProteinSEQ assays. The software is designed to work with the Applied Biosystems QuantStudio 5 Real-Time PCR Instrument with 0.1-mL, 96-well plates for either Fast or standard cycling.

Features of AccuSEQ Real-Time PCR Detection Software include:
• Automated presence/absence calls for Mycoplasma detection
• Accurate quantitation of host cell line residual DNA
• Non-linear curve fitting of ProteinSEQ assay data for Protein A
• Single software platform for all current and future SEQ real-time PCR assays
• Security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance

Mycoplasma detection
For rapid and accurate Mycoplasma detection, AccuSEQ software offers a plug-in analysis module for automated presence/absence calls when using the MycoSEQ Mycoplasma Detection system. Automated analysis tools enable one-click processing of MycoSEQ assay data, helping deliver presence or absence calls within seconds of data collection being completed.

Residual DNA quantitation
For host cell residual DNA quantitation, a custom module with next-generation algorithms can be used with the resDNASEQ CHO Residual DNA Assay for accurate quantitation.

Protein quantitation
AccuSEQ software helps enable rapid and easy analysis of ProteinSEQ protein quantitation system data without the hassle of multiple exporting steps and reliance on third party software.

Region of Interest (ROI) and Background Plates, 384-well Applied Biosystems™

These Region-of-Interest (ROI) and Background Plates are used to maintain the ViiA™ 7, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7, and QuantStudio™ 12K Flex real-time PCR systems with 384-well blocks. See the instrument maintenance guide for full instructions on use.

7500 Real Time PCR Systems Spectral Calibration Kit I Applied Biosystems™

This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR® Green I, VIC®/JOE™, and NED™/TAMRA™/ROX™ dyes on the 7500 Real-Time PCR System. The Applied Biosystems® 7500 Real-Time PCR Systems Spectral Calibration Kit I contains one background plate, one region of interest (ROI) calibration plate, and seven spectral calibration plates with seven separate dye standards.

• 96-well optical reaction plates preloaded with dyes (FAM™/SYBR® Green I, VIC®/JOE™, NED™/TAMRA™/ROX™) are provided for quick and easy calibration of the 7500 Real-Time PCR System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research, Not Instrument Calibration
This kit is easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the 7500 Real-Time PCR System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

Replacement Lamp for ViiA™ 7 Real-Time PCR System Applied Biosystems™

Replacement lamp, light source, for the ViiA™ 7 Instrument. Calibration is recommended after lamp replacement.

TaqMan™ Low Density Array Spectral Calibration Kit Applied Biosystems™

The TaqMan® Low Density Array Spectral Calibration Kit is used to validate the performance of the Applied Biosystems® 7900HT Fast Real-Time PCR Systems with the TaqMan® Low Density Array Block Upgrade. The TaqMan® Low Density Array Spectral Calibration Kit contains four empty TaqMan® Low Density Array, one background tube, and three separate pure dye standards (FAM™, ROX™,VIC®). Insert the loaded calibration arrays during system installation, and the system collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis. To ensure accurate results, we recommend that the performance of your instrument be verified every six months.

AccuSEQ™ Real-Time PCR Detection Software, version 2.1.2, seat license Applied Biosystems™

This is a seat license for AccuSEQ Real-Time PCR Detection Software v2.1.2 (Cat. No. A45662). Seat licenses are purchased for each individual user of the software.

AccuSEQ Real-Time PCR Detection Software, version 2.1.2, is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing using the SEQ family of real-time PCR assays, including MycoSEQ, resDNASEQ, and ProteinSEQ assays. The software is designed to work with the Applied Biosystems 7500 Fast Real-Time PCR Instrument with 0.1-mL, 96-well plates and Windows 10 operating system.

Features of AccuSEQ Real-Time PCR Detection Software include:
• Automated presence/absence calls for Mycoplasma detection
• Accurate quantitation of host cell line residual DNA
• Non-linear curve fitting of ProteinSEQ assay data for Protein A
• Single software platform for all current and future SEQ real-time PCR assays
• Security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance

Mycoplasma detection
For rapid and accurate Mycoplasma detection, AccuSEQ software offers a plug-in analysis module for automated presence/absence calls when using the MycoSEQ Mycoplasma Detection system. Automated analysis tools enable one-click processing of MycoSEQ assay data, helping deliver presence or absence calls within seconds of data collection being completed.

Residual DNA quantitation
For host cell residual DNA quantitation, a custom module with next-generation algorithms can be used with the resDNASEQ CHO Residual DNA Assay for accurate quantitation.

Protein quantitation
AccuSEQ software helps enable rapid and easy analysis of ProteinSEQ protein quantitation system data without the hassle of multiple exporting steps and reliance on third party software.

VeriFlex™ 384-well Temperature Verification Kit for Veriti™ System, 9 channel, 0.02 mL Applied Biosystems™

The Temperature Verification Kits and Probes are engineered and manufactured specifically for Applied Biosystems to validate temperature calibration and uniformity in GeneAmp® PCR and the Veriti® Thermal Cycler instrument systems. This product is for use with the Veriti® 384-Well Thermal Cycler.

• Self-contained digital thermometer and battery operated for ease of use
• Interchangeable Platinum Resistant probes

Complete Offering
To expand the use of an existing Temperature Verification Kits, the individual platinum resistant probes are now available in 0.02 mL, 0.1mL, 0.2 mL, for purchase.

More information can also be found at Alpha Technics (Formerly Alpha Sensors, Inc., Eutechnics Division) (www.alphatechnics.com). Calibration standards are traceable to NIST (US National Institute of Standards and Technology). The kit contains one platinum resistant probe, hand held thermometer, mineral oil, cotton swabs and carrying case.

FAM™ Dye Spectral Calibration Plate, 96-well Applied Biosystems™

This FAM™ dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

SYBR™ Green Dye Spectral Calibration Plate, 384-well Applied Biosystems™

This kit is used for SYBR® spectral calibration of your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with a 384-well block. Please see your instrument maintenance guide for full instructions on use.

These calibration kits are used to maintain your real-time PCR system with 384-well block. They include calibration plates to perform a spectral calibration with FAM™, VIC®, ROX™, SYBR® Green, TAMRA™, NED™ dyes, plates to perform region-of-interest (ROI) calibration, normalization calibration, and to run an RNase P verification. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

QuantStudio™ 3D Digital PCR Tilt Base for GeneAmp™ PCR System 9700 Applied Biosystems™

The Applied Biosystems® QuantStudio™ 3D Digital PCR Tilt Base for GeneAmp® PCR System 9700 sits beneath the GeneAmp® 9700 thermal cycler and ensures consistent performance during the PCR amplification of the QuantStudio™ 3D Digital PCR Chips. Simply place the Tilt Base beneath the feet of the thermal cycler, load your prepared 3D digital PCR chips, and begin PCR amplification.

7300 Real Time PCR Systems Spectral Calibration Kit Applied Biosystems™

This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR® Green I, VIC®/JOE™, and NED™/TAMRA™/ROX™ dyes on the Applied Biosystems® 7300 Real-Time PCR System. The Applied Biosystems® 7300 Real-Time PCR Systems Spectral Calibration Kit I contains one background plate, one region of interest (ROI) calibration plate, and seven spectral calibration plates with seven separate dye standards.

• 96-well optical reaction plates preloaded with dyes (FAM™/SYBR® Green I, VIC®/JOE™, NED™/TAMRA™/ROX™) enable you to calibrate the 7300 Real-Time PCR System quickly and easily
• Easy-to-use, preloaded spectral calibration plates reduce setup time
• Provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research, Not Instrument Calibration
This kit is easy-to-use, preloaded, 96-well optical reaction plates enable quick and easy calibration of the 7300 Real-Time PCR System. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses this calibration information to ensure accurate data analysis.

7300 System SDS v1.4 Upgrade Kit with RQ Study Applied Biosystems™

This software includes an upgrade for the 7300 SDS Software to provide the capability and reliability of the most recent version. Powerful and user-friendly, the 7300 Sequence Detection Software includes:
• Simple plate setup wizard is streamlined for each application – option to setup before or after the completing the run
• Correct plate-setup mistakes after the run without losing collected data
• One-click graphical export to PowerPoint or JPG for top notch presentations
• Flexible system can be used as a plate reader or a regular thermal cycler if needed
• Real-time amplification monitoring and flexibility to add additional PCR cycles during a run
• Lamp-lifetime monitoring ensures highest possible instrument performance

Additionally, the 7300 System SDS RQ Study Software, a gene expression analysis software package with powerful data viewing capabilities, is included. Data from up to 10 96-well RQ plates can be simultaneously analyzed. In an RQ study, you can:
• Select the endogenous control and the calibrator sample.
• Select the control type when applicable.
• Set baseline and threshold values and RQ Min/Max
Confidence Levels.
• Omit individual wells or sample replicates.

The user is able to view post-run amplification in three different formats, while gene expression plots show expression levels of target samples relative to the calibrator.

For Research Use Only. Not for use in diagnostics procedures.

7500 Fast Precision Plate Holder, for 0.1 mL tube strips Applied Biosystems™

The Applied Biosystems™ 7500 Fast Precision Plate Holder contains one precision plate holder to be used with 0.1 ml tube strips on the 7500 Fast System, and the recommended usage procedure. Note: A29252 replaces Cat. No. 4403809.

JUN™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well Applied Biosystems™

This plate is used for JUN® dye spectral calibration of your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a 384-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.
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