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AmpliTaq™ 360 DNA Polymerase Applied Biosystems™

The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. Compared to the original AmpliTaq DNA polymerase, AmpliTaq 360 polymerase is purified by an additional proprietary separation process which reduces contaminating bacterial DNA sequences from the enzyme preparation. This ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and when combined with the proprietary AmpliTaq 360 Buffer Kit, promotes the amplification of a variety of templates including those from bacterial and human genomes.

• Optimized for the broadest range of targets—from everyday to challenging
• Unmatched sensitivity and yield
• Market-leading GC enhancer for robust amplification of GC-rich sequences
• Achieves the highest quality sequencing data

For superior PCR performance, we recommend DreamTaq DNA Polymerase.

AmpliTaq Gold™ 360 DNA Polymerase Applied Biosystems™

The Applied Biosystems® AmpliTaq Gold® 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold® 360 DNA Polymerase delivers 360° coverage for a full range of targets.
* Optimized for the broadest range of targets—from everyday to challenging
* Unmatched sensitivity, specificity, and yield
* Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
* Achieves the highest quality sequencing data

Optimized for Easy and Challenging Targets

Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions (Figure 1). Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold® 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (Table 1).
As shown in Figure 1, GC-rich regions are especially poorly amplified with competitor DNA polymerases and the original AmpliTaq Gold, while AmpliTaq Gold 360 provides successful, robust amplification.

Optimized for Hot-Start PCR

AmpliTaq Gold 360 DNA Polymerase provides the same hot-start specificity as AmpliTaq Gold DNA polymerase. A high-temperature incubation step is required to activate AmpliTaq Gold DNA Polymerase, which ensures that the active enzyme is generated only at temperatures in which the DNA is fully denatured and when the primers are not annealed.
When the polymerase is added to the reaction mixture at room temperature, primer extension does not occur because the enzyme is inactivated. Any low stringency mispriming events that may have occurred will not be enzymatically extended and will not be amplified. Hence, PCR setup can be performed at room temperature without concern for extension at misprimed sites. The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. Excellent specificity across a broad range of targets is shown in Figure 1 and is summarized in Figure 2. The exquisite specificity allows easier multiplexing and allelic discrimination.
The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. Excellent specificity across a broad range of targets is shown in Figure 1 and is summarized in Figures 2A & B. The exquisite specificity allows easier multiplexing and allelic discrimination.

Superior Sensitivity and Amplification Length

Compared to the original AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates including those from bacterial and human genomes.
AmpliTaq Gold 360 DNA Polymerase efficiently amplifies targets present at low copy number (Figure 3), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection, and amplification of targets from degraded DNA samples. The extreme purity of the enzyme contributes to its unmatched sensitivity. AmpliTaq Gold 360 DNA Polymerase efficiently and reproducibly amplifies long (up to 5 Kb) sequences. Figure 4 demonstrates robust PCR amplification of long human and plasmid DNA.
 

Note:

See user's manual or package insert for limited label license, and trademark information.
For Research Use Only. Not for use in diagnostics procedures.

AmpliTaq™ 360 Buffer, 25 mM MgCl2 and 360 GC Enhancer Applied Biosystems™

The Applied Biosystems® AmpliTaq® 360 Buffer Kit is used in conjection with the AmpliTaq 360 DNA polymerase for those few experiments when the reagents supplied with the enzyme are not sufficient for the researcher's needs. When used together with or without the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq® 360 DNA Polymerase delivers 360° coverage for a full range of targets.

* Optimized for the broadest range of targets—from everyday to challenging
* Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
* Achieves the highest quality sequencing data

Optimized for Easy and Challenging Targets
Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions (Figure 1). Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold® 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (Table 1).

As shown in Figure 1, GC-rich regions are especially poorly amplified with competitor DNA polymerases and the original AmpliTaq Gold, while AmpliTaq Gold 360 provides successful, robust amplification.

Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.

Axiom™ Cotton Genotyping Array Applied Biosystems™

Axiom Cotton Genotyping Array was designed through Affymetrix- Expert Design Program, and the markers on the array were selected from public databases in collaboration with the National Botanical Research Institute, India. The array includes 35,550 markers that were discovered in G. hirsutum and G. barbadense.

Axiom Cotton Genotyping Array can be used to analyze samples from different populations by adding up to 380,000 custom markers or by transferring polymorphic markers with 100%; fidelity on to the Axiom 384HT myDesign™ breeders array. The Axiom GT1 algorithm can automatically cluster, assign genotypes, and classify the markers into six different categories for easy visualization.

Features of Axiom Cotton Genotyping Array
Comprehensive content:
The Axiom Cotton genotyping array includes a total of 35,550 markers:
• 28,158 intra-specific markers identified using gene-enriched genomic sequences of G. hirsutum.
• 7,392 markers discovered using genome reduction methodology that is based on restriction site conservation (GR-RSC).
   - 5,286 markers were discovered in an inter-specific assembly of G. hirsutum and G. barbadense.
   - 2,106 markers are intra-specific to G. hirsutum.

Applications of Axiom Cotton Genotyping Array
Complex trait research and molecular breeding:
• GWAS and QTL mapping
• Identification of economically-important traits
• Improved accuracy marker-assisted selection programs through genomic selection

Required Products
GeneChip Command Console Software
GeneTitan Multi-Channel Instrument

AmpliTaq Gold™ 360 Buffer Kit Applied Biosystems™

The AmpliTaq Gold® 360 Buffer Kit is used in conjunction with AmpliTaq Gold® 360 DNA Polymerase and is intended for those occasions when the quantity of reagents supplied with the enzyme are not sufficient. The kit includes 1.5 mL (each) of 10X AmpliTaq Gold 360 Buffer, 25 mM MgCl2, and 3360 GC Enhancer. Typically, 1.5 mL is sufficient for use with 250 U of enzyme.

TaqMan™ Fast Cells-to-CT™ Kit Invitrogen™

The TaqMan® Fast Cells-to-CT™ Kit makes it possible to use Fast-cycling, real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification.

The TaqMan® Fast Cells-to-CT Kit is:

• Complete—optimized workflow includes cell lysis reagents with gDNA removal, RT enzyme mix, RT buffer, and TaqMan® Fast Universal PCR Master Mix
• Fast—prepare samples at room temperature in 7 minutes, including DNase treatment; TaqMan® Fast Universal PCR Master Mix delivers real-time PCR results in ~35 minutes
• Convenient—lyse samples in a tube or directly in 96- or 384-well culture plates
• Robust—perform gene expression analysis on 10 to 100,000 cells per sample, with results equivalent to those from purified RNA
• Efficient—contains sufficient reagents to generate 500 real-time PCR results from 100 starting samples

Complete, validated solution
TaqMan® Cells-to-CT™ technology features a unique method for lysing cultured cells while removing genomic DNA (gDNA) and preserving RNA integrity. Additionally, the kit contains reverse transcription (RT) reagents and TaqMan® Fast Universal PCR Master Mix for Fast real-time PCR analysis, and has been extensively tested and validated with gold-standard TaqMan® Gene Expression assays, sold separately. All kit components have been optimized to work together for consistent and reliable performance, removing the guesswork involved in assembling separate kits for sample preparation, reverse transcription, and Fast real-time PCR.

Fast, convenient workflow
The TaqMan® Fast Cells-to-CT™ Kit incorporates a simple 7-minute sample preparation procedure (see figure) for use with 10 to 100,000 cultured cells directly in 96- or 384-well culture plates or in tubes. Cells are washed in PBS and lysed for 5 minutes at room temperature; an optional DNase treatment can be performed concurrently. Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution. The kit greatly simplifies a traditionally time-consuming, labor intensive process and reduces it to 7 minutes.

The lysates are now ready for reverse transcription with the optimized RT system included in the kit, or they can be stored at -20°C for up to 5 months, for future analysis. The supplied TaqMan® Fast Universal PCR Master Mix delivers sensitive and reproducible results approximately 3 times faster than standard PCR reagents and instruments. The combination of fast and simple sample preparation, and Fast-cycling real-time PCR results (~35 minutes) enables more rapid quantitation of gene targets than previously available. The TaqMan® Fast Cells-to-CT™ Kit workflow reduces the time from sample to result by 40% or more, compared to traditional RNA purification and standard real-time PCR.

Robust, sensitive, and reliable results
Accurate gene expression analysis over a wide range of sample input for genes with various expression levels has traditionally been a challenge for researchers. Therefore, a robust solution is important for profiling RNA from a few or many cells. Shown is the demonstration of the excellent signal linearity (R2 = 0.997) observed using lysates from TaqMan® Fast Cells-to-CT™ Kit across 5 logs of cellular input (10–105 cells per lysis reaction) (see figure). Thus, a single kit can be used for analysis of gene expression from limited or dense samples, with no compromise in data integrity caused by PCR inhibitors.

PCR performance of cell lysates using the TaqMan® Fast Cells-to-CT™ Kit was equivalent to that from purified RNA, using the supplied TaqMan® Fast Universal PCR Master Mix (see figure). Both TaqMan® Cells-to-CT™ Kit lysates and purified RNA showed linear detection over a 5 log range of cell input equivalents. The enhanced sensitivity at the 10-cell input with TaqMan® Fast Cells-to-CT™ Kit lysates compared to purified RNA, using the same RT and PCR reagents, results from more efficient retention of target molecules during preparation, i.e., no loss of RNA typically associated with sample transfer or heating.

The TaqMan® Fast Cells-to-CT™ Kit also demonstrated superior performance compared to purified RNA which was reverse transcribed and PCR amplified with Competitor Q’s RT and Fast Master Mix. The observed dynamic range using Competitor Q’s reagents with Fast cycling was 102 to 105 cell equivalents, or 1 log less than TaqMan® Cells-to-CT™ Kit lysates or purified RNA amplified with TaqMan® Fast Universal PCR Master Mix (see figure). The TaqMan® Fast Universal PCR Master Mix is formulated to deliver sensitive and reproducible results faster than is possible with standard reagents and instruments, and is robust across a wide range of target genes and concentrations.

Axiom™ Trout Genotyping Array (384HT format) Applied Biosystems™

Axiom Trout Genotyping Array (384HT format) was designed under the Affymetrix Expert Design Program in collaboration with the National Center for Cool and Cold Water Aquaculture, USDA-ARS, USA, and AquaGen, Trondheim, Norway. The array includes 57,501 markers and is available in both 96 format and 384 format.

Axiom Trout Genotyping Array offers a standard high-throughput, cost-effective, and robust genotyping technology to conduct genome-wide association studies (GWAS), to study genetic architecture, perform marker-trait association, and to increase accuracy of breeding programs. The high marker density on the array ensures a broad coverage of the trout genome to provide representation of all regions in the genome.

Features of Axiom Trout Genotyping Array
Comprehensive content: The array includes 57,501 markers spaced across the genome as follows:
    - 17,000 markers that are unique to SNPs discovered in a previous USDA study1
    - 20,000 markers unique to an outbred Norwegian commercial population
    - Amino acid shifting SNPs
    - SNPs preferentially located within a gene and with minor allele frequency (MAF) >0.2
    - Y chromosome-specific SNPs near the sdY gene (male-specific in rainbow trout)2

Applications of Axiom Trout Genotyping Array
Complex trait research and molecular breeding
    - GWAS and quantitative trait locus (QTL) mapping
    - Identification of economically important traits
    - Improved accuracy in aquaculture breeding programs through genomic selection

Population and evolutionary genetics
    - Development of new breeding populations
    - Differentiation between fish of different origins
    - Gender determination via sdY chromosome-specific probes
    - Differentiation of farmed and wild populations

Required Products
Axiom Analysis Suite
GeneChip Command Console Software
GeneTitan Multi-Channel Instrument

BackDrop™ Background Suppressor for Microplate Assays Invitrogen™

BackDrop® Background Suppressor is a novel reagent designed to effectively suppress background fluorescence in live cell assays. It helps improve signal-to-noise ratios when used with green-emitting fluorescent dyes or proteins.

• Can be added to complete media
• Improves sensitivity of green-emitting fluorescent dyes or proteins
• Validated with many cellular assays, including ion indicator and membrane potential assays
• Minimal cell toxicity—demonstrated to not cause osmotic shock to neuronal cells

The sensitivity of fluorescent assays can be compromised by autofluorescence or background fluorescent signals. These background signals result from cellular proteins or growth media components and contribute to a decrease in the signal-to-noise ratio and reduced assay sensitivity. Addition of BackDrop® Background Suppressor significantly reduce background signal, helping improve experimental results.

BackDrop® Background Suppressor can be added to growth media and has been formulated to not contribute to cell toxicity. It has been successfully used with many different cell types and fluorescent reagents (including ion indicators) to efficiently suppress background fluorescence.

TaqMan™ Array Human Phagocytosis of Microbes Applied Biosystems™

The Applied Biosystems® TaqMan® Array Human Phagocytosis of Microbes 96-well Plate contains 92 assays to Phagocytosis of Microbes associated genes and 4 assays to candidate endogenous control genes. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment.

• Affordable; minimum order of three plates; ideal for small, medium or large-sized projects
• Fast delivery; on your bench in days
• Convenient; dried PCR primer and TaqMan® probe sets supplied in a 96- well plate; just add master mix and your sample
• Same TaqMan®Assay Quality, New Format

TaqMan® Array Plates contain the same high quality TaqMan® Gene Expression Assays (PCR primers and TaqMan® probe sets) that have been commercially available for years in a convenient, dried, 96-well format. Extensive tests have found no difference in performance between TaqMan®Assays supplied in the traditional liquid format and assays dried into a TaqMan® Array Plate. Figure 1 shows the results of an experiment comparing the linear range of the two formats, where no difference in performance was observed.

Just Add Master Mix and Sample, and Begin Cycling
Ideal for projects such as validation of microarray and RNAi data, and pathway studies, the plates include just the right amount of TaqMan®Assay in each well: enough for one 20 µl reaction. You can order as few as three Gene Signature Plates, making these an affordable option for many laboratories and minimizing the waste of unused individually purchased TaqMan®Assays. Save a significant amount of time setting up your experiments. Just add master mix and sample, and begin cycling.

Figure 2 is an example Plate Layout with Gene Symbols and Assay IDs

Plate Map Ensures Proper Data Labeling and Easy Re-Ordering
Each pack of TaqMan® Array Plates is shipped with a CD containing the plate configuration map (see Figure 2) along with the details of each TaqMan®Assay. This information is imported into the real-time PCR instrument to enable proper labeling of experimental data and easy access to Assay ID numbers for simple reordering.

Simple to Use
Each TaqMan® Array Plate well contains a single TaqMan®Assay for one 20 µl reaction. The plates can be stored at room temperature or at 4°C. When you are ready to run your assays, simply add PCR master mix and your cDNA sample, seal the plate, and begin cycling on a real-time PCR instrument.

Compatibility
TaqMan® Array Plates have been optimized for use with TaqMan® Gene Expression Master Mix, but are also compatible with TaqMan® Universal PCR Master Mix. For studies using cultured cells, TaqMan® Array Plates are also compatible (and recommended) with the TaqMan® Gene Expression Cells-to-Ct™ Kit. TaqMan® Array Plates must be run on a real-time PCR instrument with 96-well plate capability. The plates have been validated for use on Applied Biosystems® 7000, 7300, 7500 and 7900HT Fast Real-Time PCR Systems using standard 96-well blocks, standard PCR cycling conditions, and 20 µl reaction volumes.

TaqPath™ Zika Virus Kit Applied Biosystems™

Emergency use authorization
This test has been issued Emergency Use Authorization (EUA) for TaqPath Zika Virus (ZIKV) Kit, 0.1 mL Format, by the USA FDA.
• This test has been authorized by the FDA under an EUA for use by authorized laboratories.
• This test has been authorized only for the detection of RNA from Zika virus and diagnosis of Zika virus infection, not for any other viruses or pathogens.
• This test is only authorized for the duration of the declaration that circumstances exist justifying the authorization of the emergency use of in vitro diagnostic tests for detection of Zika virus and/or diagnosis of Zika virus infection under section 564(b)(1) of the Act, 21 U.S.C. § 360bbb3(b)(1), unless the authorization is terminated or revoked sooner.

Intended use
The TaqPath Zika Virus Kit (ZIKV) is a real-time RT-PCR test intended for the qualitative detection of RNA from the Zika virus in serum and urine (collected alongside a patient-matched serum specimen) from individuals meeting Centers for Disease Control and Prevention (CDC) Zika virus clinical criteria (e.g., clinical signs and symptoms associated with Zika virus infection) and/or CDC Zika virus epidemiological criteria (e.g., history of residence in or travel to a geographic region with active Zika transmission at the time of travel, or other epidemiologic criteria for which Zika virus testing may be indicated), by laboratories in the United States that are certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA), 42 U.S.C. §263a, to perform high complexity tests, or by similarly qualified non-U.S. laboratories.

Results are for the identification of Zika virus RNA, which is generally detectable in serum and/or urine during the acute phase of infection and up to 14 days following onset of symptoms, if present. Positive results are indicative of current infection. Laboratories are required to report all positive results to the appropriate public health authorities.

Negative results do not preclude Zika virus infection and should not be used as the sole basis for patient management decisions. Negative results must be combined with clinical observations, patient history, and epidemiological information.

The TaqPath Zika Virus Kit (ZIKV) is intended for use by trained clinical laboratory personnel who have received specific training on the use of the TaqPath Zika Virus Kit (ZIKV) on the Applied Biosystems QuantStudio Dx Real-time PCR instrument and software system. The assay is only for use under the Food and Drug Administration’s Emergency Use Authorization.

About the kit
The TaqPath Zika Virus Kit (ZIKV) is a lyophilized real-time rt-pcr assay for qualitative detection of RNA from the Zika virus in serum or urine (collected alongside a patient-matched serum specimen). This kit includes primers, probes, and one-step real-time PCR master mix for the molecular detection of Zika RNA. It also includes a primer-probe set for human PPIA (cyclophilin A) as an endogenous extraction, RT-PCR, and PCR amplification control.

Features:
• Emergency Use Authorization (EUA) issued from the FDA
• Lyophilized with one-step RT-PCR master mix; simply add RNA
• Simple workflow for detection of Zika virus RNA from urine or serum
• No cold-chain management required; ship and store at room temperature

AmpliTaq Gold™ 360 Master Mix Applied Biosystems™

AmpliTaq Gold® 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package—all components are premixed and premeasured. AmpliTaq Gold® 360 Master Mix was designed for 360° coverage of a full range of targets. The master mix is supplied at 2X the recommended usage concentration for easy dilution when adding template and primers. Designed for convenience, the AmpliTaq Gold® 360 PCR Master Mix scales to various reaction volumes for greater application and format flexibility. The main ingredient of the master mix is AmpliTaq Gold® 360 DNA Polymerase but it also contains the world-class 360 GC Enhancer which can be added optionally for high GC–content templates. With the wide template coverage, optimization of PCR reaction conditions, is virtually eliminated with the use of AmpliTaq Gold® 360 Master Mix. Key features:

• Optimized for the broadest range of targets—from everyday to challenging
• Unmatched sensitivity, specificity, and yield
• Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
• Achieves the highest-quality sequencing data
• Easy-to-use, premixed master mix

Optimized for easy and challenging targets
Challenging targets include AT-rich, GC-rich, primer-dimer–forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions (see figure). Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold® 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (see table).

Optimized for automated, hot-start PCR
AmpliTaq Gold® 360 DNA Polymerase is the key ingredient in an automated, convenient, and efficient hot-start PCR. When AmpliTaq Gold® 360 PCR Master Mix is added to the reaction mixture at room temperature, the inactive enzyme is not capable of primer extension. Any low-stringency mispriming events that may have occurred will not be enzymatically extended and subsequently amplified. An initial thermal incubation step is required for activation and ensures that active enzyme is generated only at temperatures where the DNA is fully denatured. The amount of AmpliTaq Gold® 360 DNA polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. Excellent specificity across a broad range of targets (see figure). The extreme specificity allows easier multiplexing and allelic discrimination.

Amplify low-copy amplicons and long targets
AmpliTaq Gold® 360 DNA Polymerase efficiently amplifies targets present at low copy number (see figure), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection, and amplification of targets from degraded DNA samples. The extreme purity of the enzyme contributes to its unmatched sensitivity. AmpliTaq Gold® 360 DNA Polymerase efficiently and reproducibly amplifies long (up to 5 kb) sequences. The figure shown demonstrates robust PCR amplification of long human and plasmid DNA.

ABsolute QPCR Mix, SYBR Green, fluorescein Thermo Scientific™

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of ABsolute QPCR Mix, SYBR Green, and added additional capabilities for your gene expression analysis.

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Thermo Scientific ABsolute qPCR SYBR Green Mixes are optimized for SYBR Green chemistry and contain all the components necessary to perform quantitative PCR, with the exception of template and primers. The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or no reference dye. A master mix optimized for capillary instruments is also available.

Highlights

• High Sensitivity & Specificity—The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
• Optimised buffer containing SYBR Green I
• Reproducibility—All master mixes undergo extensive QC to ensure batch to batch consistency
• Broad dynamic range

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute QPCR Mix, no ROX
ABsolute QPCR Mix, low ROX
ABsolute QPCR Mix, ROX
ABsolute QPCR Capillary Mix
ABsolute QPCR Mix, SYBR Green, no ROX
ABsolute QPCR Mix, SYBR Green, low ROX
ABsolute QPCR Mix, SYBR Green, ROX
ABsolute QPCR Mix, SYBR Green, fluorescein
ABsolute QPCR Capillary Mix, SYBR Green

Phusion High-Fidelity PCR Kit Thermo Scientific™

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

Highlights

• High fidelity (52X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts
• Available as a Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Amplification of difficult (GC-rich) templates
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
• Microarray
Thermo Scientific Phusion High-Fidelity PCR Kit contains all the necessary reagents for PCR including control lambda DNA template and primers for 1.3 kb and 10 kb amplicons. The DNA template amount is sufficient for performing 20 control reactions in 50 µL volume or 50 control reactions in 20 µL volume.

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

Axiom™ Apple Genotyping Array Applied Biosystems™

Axiom Apple Genotyping Array (Axiom_Apple480) was designed through the Affymetrix Expert Design Program in collaboration with the FruitBreedomics consortium (www.FruitBreedomics.com). The sequencing and marker selection was conducted by experts from Fondazione Edmund Mach, INRA, Dalhousie University, Wageningen UR, University Di Bologna, and Universita Degli Studi Di Milano.

The 96-format array includes markers identified using whole-genome sequence data from 63 Malus domestica cultivars and two double haploid accessions. Table 1 lists the number of cultivars and corresponding country of origin used in the SNP discovery process.

Axiom Apple Genotyping Array includes 480,000 markers and together with Axiom Analysis Suite software overcomes the genotyping challenges associated with polyploidy, rapid linkage disequilibrium (LD) decay, and high polymorphism observed in the apple genome.

Array highlights
• Very high diversity
• Includes markers discovered in 63 worldwide M. domestica cultivars• High resolution to address rapid decay of LD   - 487,249 markers on the array
   - Bias towards common variants with minor allele frequency (MAF) >0.05
   - Includes 21,463 previously validated markers: 19,990 markers from an existing in-;market 20K Fruitbreedomics apple array and 1,473 markers identified using genotyping-by-sequencing (GBS)
• Absence of paralogous variants through the use of double haploid accessions in SNP discovery

Applications of Axiom Apple Genotyping Array
• Construction of high-resolution genetic maps
• Fine mapping of quantitative trait loci
• Genome-wide association studies

Required Products
GeneChip Command Console Software
GeneTitan Multi-Channel InstrumentAxiom Apple Genotyping Array (Axiom_Apple480) was designed through the Expert Design Program at Affymetrix in collaboration with the FruitBreedomics consortium (www.FruitBreedomics.com). The sequencing and marker selection was conducted by experts from Fondazione Edmund Mach, INRA, Dalhousie University, Wageningen UR, University Di Bologna, and Universita- Degli Studi Di Milano.

The 96-format array includes markers identified using whole-genome sequence data from 63 Malus domestica cultivars and two double haploid accessions. Table 1 lists the number of cultivars and corresponding country of origin used in the SNP discovery process.

Axiom Apple Genotyping Array includes 480,000 markers and together with Axiom™ Analysis Suite software overcomes the genotyping challenges associated with polyploidy, rapid linkage disequilibrium (LD) decay, and high polymorphism observed in the apple genome.

Array highlights
  • Very high diversity
  • Includes markers discovered in 63 worldwide M. domestica cultivars
  • High resolution to address rapid decay of LD
    • 487,249 markers on the array
    • Bias towards common variants with minor allele frequency (MAF) >0.05
    • Includes 21,463 previously validated markers: 19,990 markers from an existing in-market 20K Fruitbreedomics apple array and 1,473 markers identified using genotyping-by-sequencing (GBS)
  • Absence of paralogous variants through the use of double haploid accessions in SNP discovery
Applications of Axiom Apple Genotyping Array
  • Construction of high-resolution genetic maps
  • Fine mapping of quantitative trait loci
  • Genome-wide association studies

Table 1: The country of origin and the number of cultivars used in the SNP discovery process for Axiom Apple Genotyping Array.
Country of origin Number of cultivars
Australia 1
Central Europe 39
Canada 1
Iran 1
Simmental 16
Japan 1
New Zealand 1
Northern Europe 4
Russia 9
Tunisia 1
United States 5
 



TaqMan™ Array Human RHO Family GTPases Applied Biosystems™

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human RHO Family GTPases 96-well Plate contains 92 assays to RHO family GTPases associated genes and 4 assays to candidate endogenous control genes.
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