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AmpliTaq Gold™ 360 Buffer Kit Applied Biosystems™

The AmpliTaq Gold® 360 Buffer Kit is used in conjunction with AmpliTaq Gold® 360 DNA Polymerase and is intended for those occasions when the quantity of reagents supplied with the enzyme are not sufficient. The kit includes 1.5 mL (each) of 10X AmpliTaq Gold 360 Buffer, 25 mM MgCl2, and 3360 GC Enhancer. Typically, 1.5 mL is sufficient for use with 250 U of enzyme.

AmpliTaq Gold™ 360 Master Mix Applied Biosystems™

AmpliTaq Gold® 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package—all components are premixed and premeasured. AmpliTaq Gold® 360 Master Mix was designed for 360° coverage of a full range of targets. The master mix is supplied at 2X the recommended usage concentration for easy dilution when adding template and primers. Designed for convenience, the AmpliTaq Gold® 360 PCR Master Mix scales to various reaction volumes for greater application and format flexibility. The main ingredient of the master mix is AmpliTaq Gold® 360 DNA Polymerase but it also contains the world-class 360 GC Enhancer which can be added optionally for high GC–content templates. With the wide template coverage, optimization of PCR reaction conditions, is virtually eliminated with the use of AmpliTaq Gold® 360 Master Mix. Key features:

• Optimized for the broadest range of targets—from everyday to challenging
• Unmatched sensitivity, specificity, and yield
• Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
• Achieves the highest-quality sequencing data
• Easy-to-use, premixed master mix

Optimized for easy and challenging targets
Challenging targets include AT-rich, GC-rich, primer-dimer–forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions (see figure). Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold® 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (see table).

Optimized for automated, hot-start PCR
AmpliTaq Gold® 360 DNA Polymerase is the key ingredient in an automated, convenient, and efficient hot-start PCR. When AmpliTaq Gold® 360 PCR Master Mix is added to the reaction mixture at room temperature, the inactive enzyme is not capable of primer extension. Any low-stringency mispriming events that may have occurred will not be enzymatically extended and subsequently amplified. An initial thermal incubation step is required for activation and ensures that active enzyme is generated only at temperatures where the DNA is fully denatured. The amount of AmpliTaq Gold® 360 DNA polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. Excellent specificity across a broad range of targets (see figure). The extreme specificity allows easier multiplexing and allelic discrimination.

Amplify low-copy amplicons and long targets
AmpliTaq Gold® 360 DNA Polymerase efficiently amplifies targets present at low copy number (see figure), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection, and amplification of targets from degraded DNA samples. The extreme purity of the enzyme contributes to its unmatched sensitivity. AmpliTaq Gold® 360 DNA Polymerase efficiently and reproducibly amplifies long (up to 5 kb) sequences. The figure shown demonstrates robust PCR amplification of long human and plasmid DNA.

Axiom™ Equine Genotyping Array Applied Biosystems™

Axiom Equine Genotyping Array (Axiom MNEC670) for equine genotyping was designed through the Expert Design Program by Affymetrix in collaboration with the University of Minnesota and members of the Equine Genetic Diversity Consortium.*

Axiom Equine Genotyping Array is a novel array and the only array that can be used for genotyping 20 different breeds. It offers maximum utility for research in equine genetics. It features 670,796 markers that were selected through a screening of 2 million markers for optimal genomic coverage of known genetic diversity among domestic horse breeds.1 Powered by Axiom Genotyping Solution, the array offers the highest data completeness, call rates, and reproducibility of any technology available today.

Features of Axiom® Equine Genotyping Array:
• Expert design: developed by key opinion leaders in the equine community
• High resolution: available in 96 format; supporting a wide range of genotyping applications for equine research

Highlights
Content:
• Axiom Equine Genotyping Array includes a total of 670,796 markers:
   - Approximately 400,000 markers between population tag SNPs
   - Approximately 200,000 markers within population tag SNPs
   - Approximately 70,000 SNPs from legacy arrays
   - Additional SNP density within the equine major histocompatibility complex

Diversity:
Axiom Equine Genotyping Array includes markers that were selected to maximize accuracy of imputation up to 1.8 million markers both within and across 20 breeds(Fig. 1). An emphasis was placed on breeds with high commercial relevance.

Applications of Axiom Equine Genotyping Array:
• Complex trait research
   - Genome-wide analysis and fine mapping
   - Diversity analyses
   - Genomic prediction of disease risk
• Molecular breeding
   - Association mapping
   - Improved efficiency of breeding programs
   - Selection signature analyses

*The Equine Genetic Diversity Consortium (EGDC) led by the University of Minnesota represents a collaborative international community of equine researchers who are working to build a comprehensive understanding of genetic diversity among equine populations across the world. (http://www.cvm.umn.edu/equinegenetics/egdc/)

Required Products
GeneChip Command Console Software
GeneTitan Multi-Channel Instrument

TaqMan™ Array Human RHOA Pathway Applied Biosystems™

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human RHOA Pathway 96-well Plate contains 92 assays to genes associated with RHOA pathways and 4 assays to candidate endogenous control genes.

VetMAX™ BRSV PI3 Kit Thermo Scientific™

Note: The name of this product has changed. It was previously called LSI VetMAX Triplex bRSV & PI3.

Same as TaqVet Triplex bRSV & PI3 Real-Time PCR Kit

TaqMan® probes - RNA - triplex - One well reaction

Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals.

The LSI VetMAX™ kits include:

• 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for RNA kits).
• 1 external positive control of the same nature as the target (inactivated bacteria or virus). Use as a quality control for PCR and/or extraction yield.
• 1 internal positive control (exogenous) to be added at the time of extraction. Use as a quality control for extraction yield.
• A quality control certificate.

For veterinary use only.

Regulatory requirements vary by country; products may not be available in your geographic area.

Biological hazard safety

WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. All work should be conducted in properly equipped facilities using the appropriate safety equipment (for example, physical containment devices). Safety equipment also may include items for personal protection, such as gloves, coats, gowns, shoe covers, boots, respirators, face shields, safety glasses, or goggles. Individuals should be trained according to applicable regulatory and company/institution requirements before working with potentially biohazardous materials. Follow all applicable local, state/provincial, and/or national regulations. The following references provide general guidelines when handling biological samples in laboratory environment.

U.S. Department of Health and Human Services, Biosafety in Microbiological and Biomedical Laboratories (BMBL), 5th Edition, HHS Publication No. (CDC) 21-1112, Revised December 2009

World Health Organization, Laboratory Biosafety Manual, 3rd Edition, WHO/CDS/CSR/LYO/2004.11

Axiom™ Porcine Genotyping Array Applied Biosystems™

Axiom Porcine Genotyping Array (Axiom_PigHDv1) for pig genotyping was designed through the Affymetrix- Expert Design Program by Dr. Martien Groenen at Wageningen University, Netherlands, and Dr. Alan Archibald at The Roslin Institute, United Kingdom.

Axiom Porcine Genotyping Array is available in 96-array format and includes 658,692 markers discovered using whole-genome sequence data from 210 animals covering a broad range of commercial and non-commercial pig breeds that were selected for their relevance to world-wide breeding programs.1 Powered by Axiom Genotyping Solution, the array offers the highest data completeness, call rates, and reproducibility of any technology available today.

This high-density array offers the power and resolution for a wide range of applications in pig breeding and genomics that include studying marker-trait association, evaluating pure lines, identifying multi-line reference populations, as well as research applications for genome-wide analysis and selective sweep analysis studies. A key benefit of using Axiom Porcine Genotyping Array is the ability to genotype samples without experiencing marker dropout or missing data, which have been observed when using other porcine genotyping products.

Features of Axiom Porcine Genotyping Array
Expert design: developed by key opinion leaders in the porcine community
High resolution
   - 658,692 markers on the array
   - Equal spacing on chromosomes
   - Bias towards common variants
   - 56,000 of the most informative markers from an existing in-market 60,000 marker array, allowing compatibility with previous studies
High diversity: includes markers from a diverse set of commercially relevant breeds, including rare/traditional breeds

Applications of Axiom Porcine Genotyping Array
• Construction of high-resolution genetic maps
• Genetic improvement of pure lines
• Fine mapping of quantitative trait loci
• Developing training populations for calculating breeding values with genomic selection models

Required Products
GeneChip Command Console Software
GeneTitan Multi-Channel Instrument

TaqMan™ Array Human Pathogenesis of ALS Applied Biosystems™

The Applied Biosystems® TaqMan® Array Human Pathogenesis of ALS 96-well Plate contains 28 assays to pathogenesis of ALS associated genes and 4 assays to candidate endogenous control genes. All assays are plated in triplicate. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment.

• Affordable; minimum order of three plates; ideal for small, medium or large-sized projects
• Fast delivery; on your bench in days
• Convenient; dried PCR primer and TaqMan® probe sets supplied in a 96- well plate; just add master mix and your sample
• Same TaqMan®Assay Quality, New Format

TaqMan® Array Plates contain the same high quality TaqMan® Gene Expression Assays (PCR primers and TaqMan® probe sets) that have been commercially available for years in a convenient, dried, 96-well format. Extensive tests have found no difference in performance between TaqMan®Assays supplied in the traditional liquid format and assays dried into a TaqMan® Array Plate. Figure 1 shows the results of an experiment comparing the linear range of the two formats, where no difference in performance was observed.

Just Add Master Mix and Sample, and Begin Cycling
Ideal for projects such as validation of microarray and RNAi data, and pathway studies, the plates include just the right amount of TaqMan®Assay in each well: enough for one 20 µl reaction. You can order as few as three Gene Signature Plates, making these an affordable option for many laboratories and minimizing the waste of unused individually purchased TaqMan®Assays. Save a significant amount of time setting up your experiments. Just add master mix and sample, and begin cycling.

Figure 2 is an example Plate Layout with Gene Symbols and Assay IDs

Plate Map Ensures Proper Data Labeling and Easy Re-Ordering
Each pack of TaqMan® Array Plates is shipped with a CD containing the plate configuration map (see Figure 2) along with the details of each TaqMan®Assay. This information is imported into the real-time PCR instrument to enable proper labeling of experimental data and easy access to Assay ID numbers for simple reordering.

Simple to Use
Each TaqMan® Array Plate well contains a single TaqMan®Assay for one 20 µl reaction. The plates can be stored at room temperature or at 4°C. When you are ready to run your assays, simply add PCR master mix and your cDNA sample, seal the plate, and begin cycling on a real-time PCR instrument.

Compatibility
TaqMan® Array Plates have been optimized for use with TaqMan® Gene Expression Master Mix, but are also compatible with TaqMan® Universal PCR Master Mix. For studies using cultured cells, TaqMan® Array Plates are also compatible (and recommended) with the TaqMan® Gene Expression Cells-to-Ct™ Kit. TaqMan® Array Plates must be run on a real-time PCR instrument with 96-well plate capability. The plates have been validated for use on Applied Biosystems® 7000, 7300, 7500 and 7900HT Fast Real-Time PCR Systems using standard 96-well blocks, standard PCR cycling conditions, and 20 µl reaction volumes.

GeneAmp™ 10X PCR Gold Buffer & MgCl2 Applied Biosystems™

Applied Biosystems GeneAmp 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold DNA Polymerase, resulting in highly specific, robust PCR amplification.

The ionic strength and pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold DNA Polymerase.

GeneAmp 10X PCR Gold Buffer is both magnesium ion-free and gelatin-free. A separate MgCl2 solution is provided. When used together, GeneAmp 10X PCR Gold Buffer and the MgCl2 solution offer more versatility in optimizing the magnesium ion concentration to achieve optimal PCR amplification with any specific set of primers and DNA template.

AmpliTaq Gold™ 360 DNA Polymerase Applied Biosystems™

The Applied Biosystems® AmpliTaq Gold® 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold® 360 DNA Polymerase delivers 360° coverage for a full range of targets.
* Optimized for the broadest range of targets—from everyday to challenging
* Unmatched sensitivity, specificity, and yield
* Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
* Achieves the highest quality sequencing data

Optimized for Easy and Challenging Targets

Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions (Figure 1). Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold® 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (Table 1).
As shown in Figure 1, GC-rich regions are especially poorly amplified with competitor DNA polymerases and the original AmpliTaq Gold, while AmpliTaq Gold 360 provides successful, robust amplification.

Optimized for Hot-Start PCR

AmpliTaq Gold 360 DNA Polymerase provides the same hot-start specificity as AmpliTaq Gold DNA polymerase. A high-temperature incubation step is required to activate AmpliTaq Gold DNA Polymerase, which ensures that the active enzyme is generated only at temperatures in which the DNA is fully denatured and when the primers are not annealed.
When the polymerase is added to the reaction mixture at room temperature, primer extension does not occur because the enzyme is inactivated. Any low stringency mispriming events that may have occurred will not be enzymatically extended and will not be amplified. Hence, PCR setup can be performed at room temperature without concern for extension at misprimed sites. The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. Excellent specificity across a broad range of targets is shown in Figure 1 and is summarized in Figure 2. The exquisite specificity allows easier multiplexing and allelic discrimination.
The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers. Excellent specificity across a broad range of targets is shown in Figure 1 and is summarized in Figures 2A & B. The exquisite specificity allows easier multiplexing and allelic discrimination.

Superior Sensitivity and Amplification Length

Compared to the original AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates including those from bacterial and human genomes.
AmpliTaq Gold 360 DNA Polymerase efficiently amplifies targets present at low copy number (Figure 3), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection, and amplification of targets from degraded DNA samples. The extreme purity of the enzyme contributes to its unmatched sensitivity. AmpliTaq Gold 360 DNA Polymerase efficiently and reproducibly amplifies long (up to 5 Kb) sequences. Figure 4 demonstrates robust PCR amplification of long human and plasmid DNA.
 

Note:

See user's manual or package insert for limited label license, and trademark information.
For Research Use Only. Not for use in diagnostics procedures.

ABsolute QPCR Capillary Mix, SYBR Green Thermo Scientific™

Thermo Scientific ABsolute qPCR SYBR Green Mixes are optimized for SYBR Green chemistry and contain all the components necessary to perform quantitative PCR, with the exception of template and primers. The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or no reference dye. A master mix optimized for capillary instruments is also available.

Highlights

• High Sensitivity & Specificity—The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
• Optimised buffer containing SYBR Green I
• Reproducibility—All master mixes undergo extensive QC to ensure batch to batch consistency
• Broad dynamic range

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute QPCR Mix, no ROX
ABsolute QPCR Mix, low ROX
ABsolute QPCR Mix, ROX
ABsolute QPCR Capillary Mix
ABsolute QPCR Mix, SYBR Green, no ROX
ABsolute QPCR Mix, SYBR Green, low ROX
ABsolute QPCR Mix, SYBR Green, ROX
ABsolute QPCR Mix, SYBR Green, fluorescein

Phusion U Green Hot Start DNA Polymerase Thermo Scientific™

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP and read through uracil present in DNA templates.

In addition to uracil usage possibility, Phusion U features all the superior properties of other Phusion DNA Polymerases - great accuracy, speed, ability to amplify long amplicons up to 20 kb and a high specificity with Affibody based hot start. These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.

Phusion U Hot Start Green DNA Polymerase is a combination of Phusion U Hot Start DNA Polymerase and 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion U performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Highlights

Accuracy—high fidelity DNA polymerase (25x Taq)
Uracil-tolerance —engineered to incorporate dUTP and amplify uracil containing templates
Specificity—hot start for reduced nonspecific amplification and primer degradation
Speed —short extension times (15-30 s/kb)
Green format—permits direct loading of PCR products on gels

Applications

• Amplification of bisulphite-converted DNA
• Amplification of damaged or aged DNA
• Carry-over contamination control
• Uracil-excision based (USER) cloning methods

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

VetMAX™ M. bovis Kit Thermo Scientific™

Note: The name of this product has changed. It was previously called LSI VetMAX MYCOPLASMA BOVIS.

Same as TaqVet Mycoplasma bovis Real-Time PCR Kit (MPBO)

Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals.

The LSI VetMAX™ kits include:
• 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for RNA kits)
• 1 external positive control of the same nature as the target (inactivated bacteria or virus). Use as a quality control for PCR and/or extraction yield.
• A quality control certificate

For veterinary use only.

Regulatory requirements vary by country; products may not be available in your geographic area.

WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. All work should be conducted in properly equipped facilities using the appropriate safety equipment (for example, physical containment devices). Safety equipment also may include items for personal protection, such as gloves, coats, gowns, shoe covers, boots, respirators, face shields, safety glasses, or goggles. Individuals should be trained according to applicable regulatory and company/institution requirements before working with potentially biohazardous materials. Follow all applicable local, state/provincial, and/or national regulations. The following references provide general guidelines when handling biological samples in laboratory environment.

1. U.S. Department of Health and Human Services, Biosafety in Microbiological and Biomedical Laboratories (BMBL), 5th Edition, HHS Publication No. (CDC) 21-1112, Revised December 2009; found at: www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf.

2. World Health Organization, Laboratory Biosafety Manual, 3rd Edition, WHO/CDS/CSR/LYO/2004.11; found at: www.who.int/csr/resources/publications/biosafety/Biosafety7.pdf.

TaqMan™ Array Human Signaling in Gap Junctions Applied Biosystems™

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Human Signaling In Gap Junctions 96-well Plate contains 92 assays to genes associated with signaling in gap junctions and 4 assays to candidate endogenous control genes.

Luminaris Color HiGreen qPCR Master Mix, high ROX Thermo Scientific™

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris Color HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue master mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: single copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix


Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with 40X Yellow Sample Buffer and nuclease-free water.

Platinum™ Direct PCR Universal Master Mix Invitrogen™

Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA. It contains high-performing, engineered Platinum II Taq Hot-Start DNA Polymerase with dNTPs in an innovative buffer that enables universal primer annealing for superior performance in direct PCR applications.

Features of Platinum Direct PCR Universal Master Mix include:
• Direct DNA amplification from various samples due to engineered, inhibitor-tolerant Platinum II Taq Hot-Start DNA Polymerase
• Universal primer annealing temperature due to innovative buffer that enables primer annealing at 60°C
• Superior specificity, sensitivity, and yields due to Platinum hot-start technology

Platinum Direct PCR Universal Master Mix is designed to work with a variety of samples of different origins such as animal, human, plant, insect, worm, and bacterial samples. The kit includes optimized reagents to achieve superior results. The quick lysis protocol enables efficient amplification from templates up to 8 kb in size and co-cycling of different length fragments. Samples in the lysis buffer can be stored for later use. Platinum Direct PCR Universal Master Mix is provided with the optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets.

Engineered Platinum II Taq DNA polymerase confers faster cycling and tolerance to reaction inhibitors originating from sample material. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step. This automatic 'hot start' provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.

Due to the unique composition of the Platinum Direct PCR Universal Master Mix, the annealing temperature of 60°C can be used for most primer pairs that follow the general design rules. Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. Different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.

View more information about Platinum Direct PCR Universal Master Mix ›

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