PCR Supplies

PCR Supplies

A large range of products designed to facilitate various polymerase chain reaction (PCR) procedures. Products include kits, PCR reagents, dNTPs, master mixes, enzymes, primers, etc. for standard, real-time, long range, direct, high fidelity, and hot start PCR.
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1-30 of 977 Products

PCR SuperMix Invitrogen™

PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Simply add template and primers, reducing set-up time by half. PCR SuperMix contains Mg2+, dNTPs, and recombinant Taq DNA Polymerase at concentrations sufficient for routine PCR of...

SYBR™ Green PCR Master Mix Applied Biosystems™

Notification of updated SYBR Green master mix formulation: PowerTrack SYBR Green Master Mix is our newest formulation designed to greatly simplify your work and deliver high sensitivity and specificity. It comes with an optional yellow dye that can be added to your DNA sample for pipetting QC.

SuperScript IV UniPrime One-Step RT-PCR System Invitrogen™

Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube. It combines the high-processivity SuperScript IV Reverse Transcriptase (RT) with a novel UniPrime RT-PCR Master Mix to provide...

DreamTaq Green PCR Master Mix (2X) Thermo Scientific™

Thermo Scientific DreamTaq Green PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq Green buffer, MgCl2, and dNTPs. The master mix retains all features of DreamTaq DNA Polymerase.

SuperScript™ IV One-Step RT-PCR System Invitrogen™

The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.

Power SYBR™ Green PCR Master Mix Applied Biosystems™

Get superior nucleic acid quantitation with Applied Biosystems™ Power SYBR™ Green Master Mix. Power SYBR™ Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

SuperScript™ IV Reverse Transcriptase Invitrogen™

SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions. It is significantly improved over SuperScript III RT in inhibitor resistance, processivity, and reaction speed, while retaining all the benefits of that enzyme, including...

Platinum™ Taq DNA Polymerase, high fidelity Invitrogen™

Platinum™ Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum™ Taq DNA Polymerase and the proofreading (3´→5´ exonuclease activity) enzyme Pyrococcus species GB-D...

Platinum™ SuperFi™ DNA Polymerase Invitrogen™

Invitrogen™ Platinum™ SuperFi™ DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum™ hot-start technology for the highest success in PCR. Featuring >300X Taq fidelity, Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and...

dNTP Set (100 mM) Invitrogen™

Invitrogen 100 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in purified water. Each nucleotide is at a concentration of 100 mM. 100 mM dNTP mix is suitable for use in polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and...

SuperScript™ VILO™ Master Mix Invitrogen™

SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a...

Platinum™ Taq DNA Polymerase Invitrogen™

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The 'hot start' property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA...

PCR Master Mix (2X) Thermo Scientific™

Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set...

Phusion Blood Direct PCR Kit Thermo Scientific™

Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase exhibits high resistance to PCR inhibitors found in blood...

RapidFinder™ STEC Confirmation Assay Applied Biosystems™

The Applied Biosystems™ RapidFinder™ STEC Confirmation Assay is part of a complete, validated screening and confirmation workflow enabling TaqMan™ real-time PCR detection of the Shiga toxin-producing E.

Quantifiler™ Trio DNA Quantification Kit Applied Biosystems™

The Quantifiler Trio DNA Quantification Kit is a robust kit that enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human and human male DNA in a single, highly sensitive real-time PCR reaction.

TaqMan™ STEC O45 & O121 Beads, MLG Thermo Scientific™

These TaqMan™ assay beads provide a simple, reliable, and rapid method for detecting the presence of a specific bacterial or viral target. The assay uses PCR to amplify a DNA target unique to the microorganism and TaqMan™ probe to detect and quantify the target, all in an an easy-to-use, lyophilized...

TaqMan™ Verotoxin-producing Escherichia coli VT1/VT2 Multiplex Assay Beads, HPA Thermo Scientific™

These TaqMan™ assay beads provide a simple, reliable, and rapid method for detecting the presence of a specific bacterial or viral target. The assay uses PCR to amplify a DNA target unique to the microorganism and TaqMan™ probe to detect and quantify the target, all in an an easy-to-use, lyophilized...

TaqMan™ Oncorhynchus keta Assay Beads Thermo Scientific™

These TaqMan™ assay beads provide a simple, reliable, and rapid method for detecting the presence of a specific bacterial or viral target. The assay uses PCR to amplify a DNA target unique to the microorganism and TaqMan™ probe to detect and quantify the target, all in an an easy-to-use, lyophilized...

TaqMan™ RNase P Assay, ABY™ dye/QSY™ probe, primer-limited Applied Biosystems™ Green features

The Applied Biosystems™ TaqMan™ RNase P Assay with ABY™ dye-labeled QSY™ probe (primer limited) provides a pre-formulated assay for quantitating human RNase P. This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays.

TaqMan™ Fast Virus 1-Step Multiplex Master Mix for qPCR (No ROX) Applied Biosystems™

TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX) is designed to enable dye flexibility for multiplexing up to four different RNA/DNA targets for high-throughput RT-qPCR. The 4X formulation enhances detection of both RNA and DNA viral pathogens, even in the presence of challenging PCR...

M-MLV Reverse Transcriptase (200 U/μL) Invitrogen™

M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H...

T4 DNA Polymerase Invitrogen™

T4 DNA Polymerase is a DNA polymerase that has a 3´-exodeoxyribonuclease activity, but lacks 5´a3´ exodeoxyribonuclease activity. A T4 DNA Polymerase Technical Bulletin is available. Applications: Labeling double-stranded linear DNA by replacement synthesis (1).

DNA Polymerase I, Large (Klenow) Fragment Invitrogen™

DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase enzyme that lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I, but does exhibit the 5' to 3' DNA polymerase and 3' to 5' exonuclease activities. Applications: Fill-in of 5´ overhangs (1).

DNA Polymerase I Invitrogen™

DNA Polymerase I is a DNA polymerase with 5´→3´ and 3´→5´ exodeoxyribonuclease activities. DNA Polymerase I also incorporates biotinylated nucleotides. • Applications—DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5´ overhangs • Source—purified from E.

SuperScript™ III First-Strand Synthesis System Invitrogen™

The SuperScript III First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system and the amount of starting material can vary from 1 pg to 5 μg of total RNA.

Human B2M (Beta-2-Microglobulin) Endogenous Control (VIC™/TAMRA™ probe, primer limited) Applied Biosystems™ Green features

The Applied Biosystems™ Human B2M (beta-2-microglobulin) Endogenous Control (VIC™ ⁄ TAMRA Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other TaqMan™ gene expression assays.

TaqMan™ MGB Probe Applied Biosystems™ Green features

Applied Biosystems TaqMan MGB (minor groove binder) probes are dual-labeled probes used for real-time PCR applications using TaqMan chemistry. TaqMan MGB Probes incorporate a 5' fluorescent reporter dye and a 3' nonfluorescent quencher (NFQ).

AmpliTaq Gold™ DNA Polymerase, LD (Low DNA) with Gold Buffer and MgCl2 Applied Biosystems™

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase, LD (Low DNA) is a highly purified version of AmpliTaq Gold™ DNA Polymerase that significantly reduces contaminating bacterial DNA sequences commonly present in recombinant protein preparations.

TaqMan™ Array, Format 96b Applied Biosystems™

The Custom TaqMan® Array Card is a 384-well microfluidic card designed to perform 384 simultaneous real-time PCR reactions without using liquid-handling robots or multichannel pipettors. Select target assays from thousands of inventoried TaqMan® Gene Expression Assays.
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