Direct PCR Reagents and Kits

These products include various kits, master mixes, enzymes, and reagents, for direct DNA amplification from unpurified samples.
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GC-Rich PCR Performance

Isolation Technology

Overhang

GeneArt™ Genomic Cleavage Detection Kit Invitrogen™

The GeneArt Genomic Cleavage Detection Kit is a fast, T7 endonuclease I based method to quantify how well your genome editing protocol causes insertions and deletions (indels) in the genome of your cell line.

GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit (with competent cells) Invitrogen™

The GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR/Cas9 genome editing in mammalian cells with a CD4 reporter. The CD4 reporter enables bead-based enrichment, an option for magnetic bead-based...

GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit Invitrogen™

The GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit is a vector system for expression of the functional components needed for CRISPR/Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter.

SequalPrep™ Normalization Plate Kit, 96-well Applied Biosystems™

Clean up and normalize the recovery of PCR reaction products in one step with SequalPrep™ Normalization Plates. For use with 5 µl of PCR reaction, extract up to 25 ng of products per well within a 2-3 fold concentration range.

Precision gRNA Synthesis Kit Invitrogen™

The Precision gRNA Synthesis Kit is a complete system for rapid synthesis of guide RNA (gRNA) ready to complex with TrueCut™ Cas9 Protein v2 for transfection-ready Cas9 protein/gRNA ribonucleoprotein (Cas9 RNP).

RevertAid H Minus First Strand cDNA Synthesis Kit Thermo Scientific™

Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity.

CellSensor™ SIE-bla THP-1 Cell Line Thermo Scientific™

The CellSensor™ SIE-bla THP-1 cell line contains a beta-lactamase reporter gene under control of the Sis Inducible Element (SIE), stably integrated into THP-1 cells. To construct this cell line, the SIE-bla construct was transduced into THP-1 cells by lentivirus.

GeneBLAzer™ FXR-UAS-bla HEK 293T Cells Thermo Scientific™

FXR-UAS-bla HEK 293T 293 cells contain the ligand-binding domain (LBD) of the human farnesoid X receptor (FXR) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

TaqMan™ STEC STX & EAE Assay Beads, MLG Thermo Scientific™

These TaqMan™ assay beads provide a simple, reliable, and rapid method for detecting the presence of a specific bacterial or viral target. The assay uses PCR to amplify a DNA target unique to the microorganism and TaqMan™ probe to detect and quantify the target, all in an an easy-to-use, lyophilized...

TaqMan™ STEC O26 & O111 Assay Beads, MLG Thermo Scientific™

These TaqMan™ assay beads provide a simple, reliable, and rapid method for detecting the presence of a specific bacterial or viral target. The assay uses PCR to amplify a DNA target unique to the microorganism and TaqMan™ probe to detect and quantify the target, all in an an easy-to-use, lyophilized...

SuperScript™ III Platinum™ One-Step qRT-PCR Kit Invitrogen™

The SuperScript III Platinum One-Step qRT-PCR Kit’s proven formula delivers reliable, accurate target detection when it matters most. The convenient one-step format and consistent performance make it ideal for a number of gene expression applications, including infectious disease surveillance.

EXPRESS One-Step SYBR™ GreenER™ Kit, universal Invitrogen™

EXPRESS One-Step SYBR GreenER kits are compatible with fast-cycling instruments for your SYBR-based qPCR experiments. This kit comes with a separate tube of ROX reference dye. EXPRESS One-Step SYBR GreenER SuperMix kits with premixed ROX reference dye are also available.

SuperScript™ IV First-Strand Synthesis System Invitrogen™

The SuperScript™ IV First-Strand Synthesis System for RT-PCR is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA. The kit includes SuperScript™ IV Reverse Transcriptase (RT), a proprietary MMLV mutant that produces robust and reliable cDNA synthesis in RT reactions.

Verso 1-step RT-qPCR Kit, with separate ROX vial Thermo Scientific™

The Thermo Scientific Verso 1-step RT-qPCR Kit for probe chemistry offers a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

CLA GlobalFiler™ PCR Amplification Kit Applied Biosystems™

The CLA (Cell Line Authentication) GlobalFiler PCR Amplification Kit simplifies the amplification step during authentication of cell lines. GlobalFiler PCR amplification kits are 6-dye, 24-locus short tandem repeat (STR) analysis kits that combine maximum compatibility with global databasing loci...
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