Hot Start PCR Reagents and Kits

Hot start PCR reduces non-specific DNA amplification that occurs at room or colder temperatures by preventing key reagents from reacting until optimal temperatures are reached. These products include kits, master mixes, polymerase, and buffers, that require thermal activation and can be used to increase product yield, specificity, and sensitivity.
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Platinum™ II Taq Hot-Start DNA Polymerase Invitrogen™

Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.

Platinum™ II Green PCR Buffer (5X) Invitrogen™

Invitrogen Platinum II Green PCR Buffer is an innovative buffer optimized for use with Platinum II Taq Hot-Start DNA Polymerase. Due to its unique composition, the annealing temperature is 60°C for most primer pairs designed following the general design rules.

Platinum™ II Taq Hot-Start DNA Polymerase Invitrogen™

Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.

Platinum™ II Taq Hot-Start DNA Polymerase Invitrogen™

Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.

AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2 Applied Biosystems™

AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation. Features of this enzyme: • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield • Time-released thermal activation improves sensitivity in low copy...

AmpliTaq Gold™ DNA Polymerase with Buffer I Applied Biosystems™

AmpliTaq Gold™ DNA Polymerase is a chemically modified form of AmpliTaq™ DNA Polymerase requiring thermal activation. Features of this enzyme: • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield • Time-released thermal activation improves sensitivity in low copy...

AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2 Applied Biosystems™

AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation. Features of this enzyme: • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield • Time-released thermal activation improves sensitivity in low copy...

GeneAmp™ 10X PCR Gold Buffer & MgCl2 Applied Biosystems™

Applied Biosystems GeneAmp 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold DNA Polymerase, resulting in highly specific, robust PCR amplification. The ionic strength and pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation...

Platinum™ Direct PCR Universal Master Mix Invitrogen™

Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA. It contains high-performing, engineered Platinum II Taq Hot-Start DNA Polymerase with dNTPs in an innovative buffer that enables universal primer annealing for...

Platinum™ II Hot-Start PCR Master Mix (2X) Invitrogen™

Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup. Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique...

Platinum™ II Hot-Start Green PCR Master Mix (2X) Invitrogen™

Invitrogen Platinum II Hot-Start Green PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup, as well as two tracking dyes for direct loading of PCR products on gels.

Phusion™ Hot Start II DNA Polymerase (2 U/µL) Thermo Scientific™

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high...

Phire Hot Start II PCR Master Mix Thermo Scientific™

Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.

Phire Green Hot Start II PCR Master Mix Thermo Scientific™

Thermo Scientific Phire Green Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.

Phire Hot Start II DNA Polymerase Thermo Scientific™

Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It outperforms every Taq-based hot start polymerase on the market. Phire Hot Start II DNA Polymerase is significantly faster, extremely robust, and also capable of...
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