Shop All Real Time PCR Master Mixes

TaqPath™ 1-Step Multiplex Master Mix (No ROX) Applied Biosystems™

TaqPath 1-Step Multiplex Master Mix (No ROX) is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction. Particularly useful for diagnostics, virus detection, and high throughput gene expression workflows, the mix includes thermostable MMLV reverse transcriptase, dNTPs, UNG, and thermostable Fast DNA polymerase all in a single tube. TaqPath is manufactured under a strong ISO13485 quality management system and is designed to produce consistent product performance lot after lot.

Request an evaluation sample >

This single-tube, 4X format facilitates easy reaction set up—just add user-supplied assay and sample (Figure 1). With reproducible performance even in the presence of inhibitors, coupled with stringent quality control to minimize lot-to-lot variation, TaqPath 1-Step Multiplex Master Mix (No ROX) is a superior choice for your multiplex diagnostic testing or development needs.

Features of the TaqPath 1-Step Multiplex Master Mix include:
• High sensitivity to detect low-copy targets with reproducible CT results
• Six logs of dynamic range with both RNA and DNA targets
• Optimized for multiplexing up to four targets simultaneously without a passive reference dye
• Tolerant of inhibitors commonly found in clinical samples

High sensitivity
To enable reproducible detection of low-titer pathogen and transcripts, TaqPath 1-Step Multiplex Master Mix (No ROX) has been optimized as a higher-concentration 4X master mix that allows you to input more sample into each reaction, increasing sensitivity even in low-volume reactions. Figure 1 shows the consistency of results obtained when detecting 10 copy inputs of RNA target.

Wide dynamic range compatible with RNA and DNA
TaqPath 1-Step Multiplex Master Mix (No ROX) has been optimized to provide high specificity and dynamic range for use with both RNA and DNA targets. Since virology labs often test for both RNA and DNA viruses, TaqPath 1-Step Multiplex Master Mix (No ROX) is designed to use a single protocol to assay both types of nucleic acid. This input flexibility can help streamline the number of different workflows in your lab to improve efficiency. Figure 2 demonstrates the excellent PCR linearity across a 6-log RNA dilution series, while Figure 3 exhibits a 6-log linear dynamic range for both RNA and DNA targets.

Optimized for multiplexing
TaqPath 1-Step Multiplex Master Mix has been validated for multiplexing up to four targets simultaneously, allowing for additional targets and/or controls to be run simultaneously for efficiency or quality control purposes. The mix does not include a passive reference dye, enabling the measurement of the JUN dye, or a similar emission wavelength dye, in the channel previously used to measure ROX dye. Figure 3 demonstrates TaqPath 1-Step Multiplex Master Mix (No ROX) performance in 4-plex reactions with both RNA and cDNA targets.

Inhibitor tolerant
Unlike other master mixes on the market, the unique proprietary formulation of TaqPath 1-Step RT-qPCR Master Mix (No ROX) allows robust performance even in the presence of substances that can normally inhibit PCR, such as heparin, hematin, or EDTA, increasing your confidence when working with a variety of complex clinical samples. TaqPath master mixes demonstrate increased tolerance of inhibitors compared to competitor mixes (Figure 4).

Manufacturing production and process controls
TaqPath 1-Step Multiplex Master Mix (No ROX) is manufactured under a strong ISO13485 quality management system that uses traceable quality raw materials and validated operating procedures. With established controls from purchasing through QC release, TaqPath product manufacturing is designed to produce consistent product performance lot after lot. Figure 5 demonstrates CT lot-to-lot consistency is preserved across multiple assays with different attributes and input target levels.

Broad instrument compatibility
The TaqPath 1-Step Multiplex Master Mix (No ROX) formulation can be run in either fast or standard cycling conditions with equivalent performance across a wide variety of real-time PCR systems including the Applied Biosystems 7500, 7500 Fast, 7500 Fast Dx, QuantStudio 3, 5, 6, 7, 12k, Dx, and ViiA 7 real-time PCR systems.

General purpose reagent
TaqPath 1-Step RT-qPCR Master Mix is a General Purpose Reagent (GPR), labeled for laboratory use.

Related products
TaqPath 1-Step Master Mix is available in three different formulations:
TaqPath 1-Step qRT-PCR Master Mix—includes ROX dye as the passive reference
TaqPath 1-Step Multiplex Master Mix—includes MUSTANG PURPLE dye as the passive reference
TaqPath 1-Step Multiplex Master Mix (no ROX) (this page)—does not include a passive reference dye

Maxima SYBR Green/ROX qPCR Master Mix (2X) Thermo Scientific™

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Maxima SYBR Green qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).

Highlights

SpecificityMaxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity—detects low copy number targets
Wide linear range—accurate quantification across 9 orders of magnitude
Reproducibility and convenience—ready-to-use 2x master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Maxima SYBR Green/ROX qPCR Master Mix (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water.

Related Products
Maxima SYBR Green/ROX qPCR Master Mix (2X)
Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

EXPRESS SYBR™ GreenER™ qPCR Supermix, with premixed ROX Invitrogen™

Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of EXPRESS SYBR GreenER SuperMix and added additional capabilities for your gene expression analysis.

EXPRESS SYBR GreenER qPCR SuperMix kits are compatible with fast-cycling instruments for your SYBR-based qPCR experiments. This EXPRESS SYBR GreenER qPCR SuperMix comes with premixed ROX reference dye. EXPRESS SYBR GreenER qPCR Supermix Universal, with separate ROX reference dye, is also available.

This kit provides:
• Fast-activating, antibody-mediated Platinum™ Taq DNA Polymerase
• SYBR GreenER dye
• UDG carryover protection—uracil-DNA-glycosylase (UDG)/dUTP to reduce carryover contamination

SYBR™ Select Master Mix for CFX Applied Biosystems™

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Select Master Mix for CFX and added additional capabilities for your gene expression analysis.

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SYBR® Select Master Mix for CFX is a superior and cost-effective solution for your real-time PCR applications.

• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR® GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• For use with the Bio-Rad CFX96™ Touch or CFX384™ Touch systems
• Standard and fast cycling parameters

Formulated for Maximum Specificity
SYBR® Select Master Mix for CFX contains all the components needed for your real-time PCR reactions, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq® DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.

Obtain Reproducible Results Across a Wide Dynamic Range
SYBR® Select Master Mix for CFX is specially formulated to provide robust results from 100 ng to 0.1 pg cDNA per reaction (Figure 1) and can reliably detect a single copy of genomic DNA (Figure 2).

Contains SYBR® GreenER™ Dye and Heat-labile UDG
• SYBR® GreenER™ dye is less inhibitory to PCR than SYBR® Green I dye, resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

View performance data.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

TaqPath™ qPCR Master Mix, CG Applied Biosystems™

The master mix delivers confident results in both single and duplex reactions, even in the presence of inhibitors commonly found in clinical samples. All lots are functionally tested to help ensure lot-to-lot reproducibility for Ct consistency and dynamic range across a wide variety of assays. This General Purpose Reagent is manufactured according to applicable CFR requirements and labeled 'For Laboratory Use'. With stringent quality and premier performance, TaqPath qPCR Master Mix is a superior choice for your diagnostic testing or development needs.

Features of the TaqPath qPCR Master Mix include:
• Efficient and linear detection of up to eight logs with gene expression or miRNA assays
• Enables reliable detection of low-copy templates with reproducible CT results
• Robust multiplexing performance with exogenous or endogenous targets
• Manufactured with stringent production and process controls to help ensure lot-to-lot consistency

TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.

Validated with a breadth of workflows
TaqPath qPCR Master Mix, CG, has been validated to provide high specificity and dynamic range for use in multiple real-time PCR applications. The formulation can be run in either fast or standard cycling conditions on a wide variety of qPCR platforms. The figure below demonstrates the excellent PCR linearity over an 8-log input range of template with both gene expression and miRNA assays.

In addition, TaqPath qPCR Master Mix, CG, has been engineered to retain consistent performance in preassembled reactions for up to 48 hours. The stability of this mix provides users of high-throughput, liquid handling systems the assurance that the results on the first plate will mimic those of the last plate. TaqPath qPCR Master Mix has been benchmarked against similar competitor master mixes and demonstrates equivalent or better sensitivity and dynamic range across a variety of targets.

Reproducible sensitive detection
We understand the importance of reliably detecting low-copy targets for your test quality and data interpretation. TaqPath qPCR Master Mix, CG, helps generate significant and reproducible CT values for ≤10 copy detection. The figure below shows the consistent CT results obtained from three unique lots when detecting 10-copy inputs. This lot-to-lot CT consistency is preserved across multiple assays with different attributes and expression levels to maximize confidence in your results.

Optimized for multiplexing
Simultaneous amplification of multiple assays can be beneficial not only as a control to normalize and detect anomalies in experiments, but also to improve efficiency and cost-savings for labs. TaqPath qPCR Master Mix has been optimized with enzymes and component concentrations to facilitate multiplexing while preserving specificity, and it is validated for duplex performance in each manufacturing lot.

Inhibitor tolerant
Unlike other master mixes on the market, TaqPath qPCR Master Mix’s unique proprietary formulation allows robust performance even in the presence of substances that can normally inhibit PCR, such as heparin, hematin, or EDTA, increasing your confidence when working with a variety of complex clinical samples. TaqPath master mixes demonstrate increased tolerance to inhibitors compared to competitor mixes.

SYBR™ Select Master Mix Applied Biosystems™

SYBR® Select Master Mix is a superior and cost-effective solution for your real-time PCR applications.

Features of the SYBR® Select Master Mix include:

• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR® GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• Can be used in either standard or fast cycling mode

Formulated for Maximum Specificity
SYBR® Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq® DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.

Obtain Reproducible Results Across a Wide Dynamic Range
SYBR Select Master Mix is specially formulated to provide robust results at 100 ng to 0.1 pg cDNA per reaction (Figure 1) and can reliably detect a single copy of genomic DNA (Figure 2).

Contains SYBR® GreenER™ Dye and Heat-labile UDG
• SYBR® GreenER™ dye is less inhibitory to PCR than SYBR® Green I dye resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

Instrument Compatibility
SYBR® Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments except the 7900HT real-time PCR system. It is also compatible with the Bio-Rad IQ™5, Roche LightCycler® LC480, and Stratagene® MX3005P® systems.

View performance data.

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Select Master Mix and added additional capabilities for your gene expression analysis.

TaqPath™ 1-Step Multiplex Master Mix Applied Biosystems™

TaqPath 1-Step Multiplex Master Mix is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction. Particularly useful for diagnostics, virus detection, and high throughput gene expression workflows, the mix includes thermostable MMLV reverse transcriptase, dNTPs, UNG, MUSTANG PURPLE dye (passive reference), and thermostable Fast DNA polymerase all in a single tube. TaqPath is manufactured under a strong ISO13485 quality management system and is designed to produce consistent product performance lot after lot.

This single-tube, 4X format facilitates easy reaction set up—just add user-supplied assay and sample (Figure 1). With reproducible performance even in the presence of inhibitors, coupled with stringent quality control to minimize lot-to-lot variation, TaqPath 1-Step Multiplex Master Mix is a superior choice for your multiplex diagnostic testing or development needs.

Features of the TaqPath 1-Step Multiplex Master Mix include:
• High sensitivity to detect low-copy targets with reproducible CT results
• Broad dynamic range with both RNA and DNA targets
• Optimized for multiplexing up to four targets simultaneously with MUSTANG PURPLE reference dye
• Tolerant of inhibitors commonly found in clinical samples
• Advanced manufacturing production and process controls insure lot-to-lot consistency

High sensitivity
To enable reproducible detection of low-titer pathogen and transcripts, TaqPath 1-Step Multiplex Master Mix has been optimized as a higher-concentration 4X master mix that allows you to input more sample into each reaction, increasing sensitivity even in low-volume reactions. Figure 1 shows the consistency of results obtained when detecting 10 copy inputs of RNA target.

Wide dynamic range compatible with RNA and DNA
TaqPath 1-Step Multiplex Master Mix has been optimized to provide high specificity and dynamic range for use with both RNA and DNA targets. Since virology labs often test for both RNA and DNA viruses, TaqPath 1-Step Multiplex Master Mix is designed to use a single protocol to assay both types of nucleic acid. This input flexibility can help streamline the number of different workflows in your lab to improve efficiency. Figure 2 demonstrates the excellent PCR linearity across a 6-log RNA dilution series, while Figure 3 exhibits a 6-log linear dynamic range for both RNA and DNA targets.

Optimized for multiplexing
TaqPath 1-Step Multiplex Master Mix has been validated for multiplexing up to four targets simultaneously, allowing for additional targets and/or controls to be run simultaneously for efficiency or quality control purposes. Rather than including ROX dye as a passive reference, the TaqPath 1-step Multiplex Master Mix includes the longer emission wavelength MUSTANG PURPLE dye as a passive reference. This longer wavelength passive reference dye enables measurement of the JUN dye, or a similar emission wavelength dye, in the channel previously used to measure ROX dye. Figure 3 demonstrates TaqPath 1-Step Multiplex Master Mix performance in 4-plex reactions with both RNA and cDNA targets.

Inhibitor tolerant
Unlike other master mixes on the market, the unique proprietary formulation of TaqPath 1-Step RT-qPCR Master Mix allows robust performance even in the presence of substances that can normally inhibit PCR, such as heparin, hematin, or EDTA, increasing your confidence when working with a variety of complex clinical samples. TaqPath master mixes demonstrate increased tolerance of inhibitors compared to competitor mixes (Figure 4).

Manufacturing production and process controls
TaqPath 1-Step Multiplex Master Mix is manufactured under

EXPRESS One-Step SYBR™ GreenER™ Kit, with premixed ROX Invitrogen™

EXPRESS One-Step SYBR GreenER kits are compatible with fast-cycling instruments for your SYBR-based qPCR experiments. This kit comes with premixed ROX reference dye. EXPRESS One-Step SYBR GreenER SuperMix kits with a separate tube of ROX reference dye are also available.

This kit provides:
• Fast-activating, antibody-mediated Platinum™ Taq DNA Polymerase
• The One-Step SuperScript™ III Reverse Transcriptase for one-step qRT-PCR
• SYBR GreenER dye
• UDG carryover protection—uracil-DNA-glycosylase (UDG)/dUTP to reduce carryover contamination

PowerUp™ SYBR™ Green Master Mix Applied Biosystems™

PowerUp™ SYBR® Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUp™ SYBR® Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.

PowerUp™ SYBR® Green Master Mix features include:
• A dual hot-start mechanism for excellent specificity
• Highly reproducible CTs over a broad dynamic range
• Inclusion of UDG to help prevent carryover contamination
• Stability of pre-assembled reactions for up to 72 hours
• Compatibility with most real-time qPCR instruments

Exceptional specificity with dual hot-start mechanism
Specificity is paramount in obtaining high-quality data in SYBR® Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding. PowerUp™ SYBR® Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures. In an experiment evaluating 24 different primer sets, PowerUp™ SYBR® Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification.

Tight reproducibility in CTs over a wide dynamic range
PowerUp™ SYBR® Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested (see figure). In addition, this master mix provides efficient amplification over 6 logs of sample input. Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR® Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality (see figure).

UDG included for carryover contamination control
The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR® Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives.

Stability of pre-assembled reactions
The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR® Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data (see figure). This consistency allows users to have confidence and flexibility in the use of PowerUP™ SYBR® Green Master Mix across numerous workflow scenarios1.

Broad instrument compatibility
PowerUp™ SYBR® Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments2. It is also compatible with the Bio-Rad IQ™5 and CFX96™ / CFX384™ systems, Roche LightCycler® LC480, and Stratagene® MX3005P® systems. For optimal results, recommended primer concentrations are 300–800 nM.

Learn more about PowerUp™ SYBR® Green Master Mix >

1 Pre-PCR stability is influenced not only by the master mix, but also by the target being analyzed. For maximum confidence, researchers should confirm stability profiles of their specific targets.
2 For optimal performance, standard cycling using the Applied Biosystems® 7900 system is recommended.

Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial Thermo Scientific™

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Maxima SYBR Green qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).

Highlights

SpecificityMaxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity—detects low copy number targets
Wide linear range—accurate quantification across 9 orders of magnitude
Reproducibility and convenience—ready-to-use 2x master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Maxima SYBR Green qPCR Master Mix, ROX Solution provided (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer. Supplied with nuclease-free water and a separate ROX vial.

Related Products
Maxima SYBR Green/ROX qPCR Master Mix (2X)
Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)
Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Luminaris Color Probe qPCR Master Mix, low ROX Thermo Scientific™

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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Luminaris Color Probe qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color Probe qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue Master Mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
• Sensitivitity—single copy detection
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience—ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color Probe Low ROX qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with 40X Yellow Sample Buffer and nuclease-free water.

Path-ID™ qPCR Master Mix with Manual Applied Biosystems™

Ambion® Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan® probe-based, real-time PCR detection of DNA targets. It is optimized for targets of importance in animal health and to function in the presence of PCR inhibitors. The mix is supplied in one tube suitable for 100 reactions. With the exception of PCR primers and probes for your target, the master mix contains everything you need for real-time PCR, including:
• AmpliTaq Gold® ultrapure thermostable DNA polymerase designed for automatic hot start, to minimize nonspecific PCR products
• Optimized buffer and dNTP concentration, for reliable PCR
• Passive internal reference, based on proprietary ROX™ dye, for precise data analysis.

Luminaris Color Probe qPCR Master Mix, high ROX Thermo Scientific™

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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Luminaris Color Probe qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Luminaris Color Probe and Luminaris Probe qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added.

The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color Probe qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color Probe and Luminaris Probe qPCR Master Mixes with ROX at different concentrations as passive reference dye are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue Master Mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Specificity—Hot Start Taq DNA Polymerase and the optimized buffer reduce primer dimers
• Sensitivitity—single copy detection
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience— ready-to-use 2X master mix
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX for different real-time platforms
• Standard cycling protocols

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color Probe High ROX qPCR Master Mix (2X) includes Hot Start Taq Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with 40X Yellow Sample Buffer and nuclease-free water.

ABsolute QPCR Mix, SYBR Green, low ROX Thermo Scientific™

Thermo Scientific ABsolute qPCR SYBR Green Mixes are optimized for SYBR Green chemistry and contain all the components necessary to perform quantitative PCR, with the exception of template and primers. The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or no reference dye. A master mix optimized for capillary instruments is also available.

Highlights

• High Sensitivity & Specificity—The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
• Optimised buffer containing SYBR Green I
• Reproducibility—All master mixes undergo extensive QC to ensure batch to batch consistency
• Broad dynamic range

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

Related Products
ABsolute QPCR Mix, no ROX
ABsolute QPCR Mix, low ROX
ABsolute QPCR Mix, ROX
ABsolute QPCR Capillary Mix
ABsolute QPCR Mix, SYBR Green, no ROX
ABsolute QPCR Mix, SYBR Green, low ROX
ABsolute QPCR Mix, SYBR Green, ROX
ABsolute QPCR Mix, SYBR Green, fluorescein
ABsolute QPCR Capillary Mix, SYBR Green

Maxima Probe/ROX qPCR Master Mix (2X) Thermo Scientific™

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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Maxima Probe qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (see Reference 1). The use of Maxima Probe qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates.

Maxima Probe qPCR Master Mixes are compatible with most real-time thermal cyclers.

Highlights

• Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity—detects low copy number targets
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience—ready-to-use 2X master mix

Applications

• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes
Maxima Probe/ROX qPCR Master Mix (2X) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water.

Related Products
Maxima Probe/ROX qPCR Master Mix (2X)
Maxima Probe qPCR Master Mix (2X), with separate ROX vial
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