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Custom TaqMan™ Small RNA Assay Applied Biosystems™

Custom TaqMan Small RNA Assays use TaqMan Real-Time PCR Assays for the analysis of any small RNA molecule. The novel adaptations in TaqMan Assay design developed for the study of miRNAs are ideal for analysis of any small nucleic acid less than 200 bases long, including newly discovered miRNAs, Piwi-interacting RNA (piRNA), small nuclear RNA (snRNA), and small nucleolar RNA (snoRNA).

Flexible and convenient ordering—easy process with our secure Custom TaqMan Small RNA Assay design tool
Versatile application—use for the analysis of any small RNA from any species
Reliable outcome—pre-formulated assays are optimized to run under universal conditions
Powerful miRNA quantitation—the same sensitivity, specificity, and reproducibility as predesigned TaqMan MicroRNA Assays

Sophisticated design pipeline
The miRPipe Small RNA Assay design pipeline is founded on our extensive background and bioinformatics expertise designing TaqMan Gene Expression Assays. To detect very small targets, it includes design features based on empirical data gathered over years of experiments. The resulting automated pipeline includes built-in flexibility features enabling design of assays for the broadest assortment of small RNA sequences. The benefits of TaqMan Assay technology are ideal for analysis of virtually any small RNA, enabling results you can trust from the very first experiment.

Ordering information
First, bioinformatically qualify your sequence target for specificity and sequence quality, then use the Custom TaqMan Small RNA Assay Design Tool to input your sequence and submit it for assay design. This easy-to-use tool is a secure and confidential. If we are unable to design or manufacture an assay for your sequence target of interest, you will not be charged.

TaqMan™ Gene Expression Assay, VIC primer-limited Applied Biosystems™

Applied Biosystems TaqMan Gene Expression Assays, VIC primer-limited, are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5’ end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3’ end. These assays are primer-limited and ideal for multiplex reactions looking at two different gene targets in the same qPCR well.

Features include:
Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
Accurate—identify small fold-changes with high accuracy of quantitation
Extensive content—over 1.8 million predesigned assays available for over 25 different species
Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology
Ideal for multiplexing—combine one FAM-labeled and one VIC-labeled assay to create a duplex assay for two different gene targets in the same qPCR well
Ideal for control assays—use a VIC primer-limited assay for high-expressing endogenous control genes

Approximate ship time
Made to order: 4–6 days in North America, 6–10 days in Europe

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents. All of our predesigned TaqMan Gene Expression assays are covered by the TaqMan Assays qPCR Guarantee.*

Recommended master mix: TaqMan Fast Advanced Master Mix

*Subject to terms and conditions. For complete details, go to www.thermofisher.com/taqmanguarantee.

TaqMan™ GAPDH Assay, JUN™ dye/QSY™ probe Applied Biosystems™

The Applied Biosystems® TaqMan® GAPDH Assay with JUN® dye-labeled QSY® probe provides a pre-formulated assay for quantitating human GAPDH. This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays. The assay consists of a JUN® dye-labeled QSY® probe plus sequence-specific forward and reverse primers, and it can be used for multiplex or singleplex PCR reactions.

Benefits of this assay include:

• Eliminate assay design time by using pre-designed primers and pre-designed JUN® dye-labeled TaqMan® probe
• Minimize experimental optimization time
• Use in conjunction with FAM™ or VIC® dye-labeled predesigned gene expression assays

Save time in assay design, formulation, and testing by using the TaqMan® GAPDH Assay as your control when quantifying gene expression. This pre-designed probe and primer set enables you to normalize the amount of sample RNA or DNA in a reaction when used with a control sample. Or use it for relative gene expression quantification in cDNA samples when used with other gene expression assays.

The JUN® dye is optimized for use with the 4th filter of our QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments. Please note that master mix containing ROX™ dye cannot be used with this assay (with JUN® dye) due to an overlap of spectra. TaqMan® Multiplex Master Mix with MUSTANG PURPLE® dye is recommended.

TaqMan™ Gene Expression Cells-to-CT™ Kit Invitrogen™

The TaqMan® Gene Expression Cells-to-CT™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification. This kit saves time and offers a simple workflow that is suitable for a few samples or can be easily incorporated into automated, high-throughput applications.

The TaqMan® Gene Expression Cells-to-CT Kit is:

• Complete—optimized workflow includes cell lysis reagents with gDNA removal, RT enzyme mix, buffer, and new TaqMan® Gene Expression Master Mix
• Fast—7-minute sample prep, including DNase treatment, at room temperature
• Easy—lyse samples in a tube or directly in culture plates
• Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
• Efficient—contains sufficient reagents to generate 500 real-time PCR results from 100 starting samples

Featuring a unique method for lysing cultured cells while removing genomic DNA and preserving RNA integrity, the TaqMan® Gene Expression Cells-to-CT™ Kit contains reverse transcription (RT) reagents for cDNA synthesis and TaqMan® Gene Expression Master Mix for real-time PCR analysis. TaqMan® primer⁄probe sets are sold separately.

Simple 7-minute sample preparation: part of a complete workflow
Whether you are using plates or tubes, the TaqMan® Gene Expression Cells-to-CT™ Kit, which uses the simple 7-minute sample preparation procedure outlined (see figure), is designed for 10–100,000 cultured cells⁄sample. Cells are washed in PBS and lysed in solution for 5 minutes at room temperature; DNase treatment can be performed simultaneously. Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution. The lysates are now ready for reverse transcription or storage at –20°C. Because samples can be processed directly in culture wells (96 or 384 wells), sample handling and the potential for sample loss or transfer error are minimized, facilitating rapid, high-throughput processing. Unlike old-fashioned, multi-step RNA isolation protocols, no heating, washing, or centrifugation steps are required. The kit greatly simplifies a laborious 30–60 minute process and reduces it to 7 minutes.

The TaqMan® Gene Expression Cells-to-CT™ Kit workflow enables unsurpassed gene expression evaluation with any of the >700,000 TaqMan® Gene Expression Assays. This kit has been extensively tested for specificity with a broad selection of TaqMan® Gene Expression Assays and shows performance equivalent to that obtained with purified RNA (see figure).

Achieve unsurpassed performance and sensitivity
Unlike some competitor kits that limit the amount of lysate in the RT reaction to 5%, the TaqMan® Gene Expression Cells-to-CT™ Kit can accommodate 45% of the total RT reaction volume as cell lysate. Additionally, cDNA can comprise up to 45% of the real-time PCR reaction volume. The large lysate volume in the optimized RT reaction, along with the large cDNA volume in the subsequent real-time PCR using the TaqMan® Gene Expression Master Mix, lead to maximum sensitivity. The master mix amplifies the target precisely and accurately, enabling the detection of small quantities of target, such as transcripts expressed at low levels.

The ability to detect limited target quantities was tested by mixing a constant number of human cells with various numbers of mouse cells. The cell mixtures were prepared using the TaqMan® Gene Expression Cells-to-CT™ and assayed for a mouse-specific and human-specific gene. Comparative data were generated in the same manner with RNA purified by traditional methodology. The data show that RNA from as few as 10 mouse cells was detectable in a background of RNA from 10,000 human cells (see figure). The ability of the TaqMan® Gene Expression Cells-to-CT™ Kit to detect relative low abundance transcripts was equivalent to that of purified RNA; at low levels, it was superior.

Additionally, the performance of the TaqMan® Gene Expression Cells-to-CT™ Kit was compared to competitor lysate kits and to purified RNA. Inputs of 100–100,000 cells⁄lysis reaction were examined. The sensitivity of the TaqMan® Cells-to-CT™ Kit protocol was equivalent to that obtained with purified RNA, and it surpassed competitor lysates (see figure). Furthermore, the lack of inhibition at high cellular inputs and the low variation among technical replicates demonstrate the reliability of this approach for gene expression studies using cultured cells.

Proven performance, proven together
All components of the TaqMan® Gene Expression Cells-to-CT™ Kit have been optimized for consistent and reliable performance. This removes the guesswork involved in assembling separate sample preparation, RT, and real-time PCR kits. And the TaqMan® Gene Expression Cells-to-CT™ Kit has been validated with TaqMan® Gene Expression Assays for added quality assurance.

Accessory product
The TaqMan® Cells-to-CT™ Control Kit was designed for use with the TaqMan® Gene Expression Cells-to-CT™ Kit. This kit contains an endogenous control (ACTB) to normalize for sample loading variability and an artifical XenoRNA™ control and corresponding TaqMan® Gene Expression Assay to monitor the effects of PCR inhibition.

TaqMan™ GAPDH Assay, JUN™ dye/QSY™ probe, primer-limited Applied Biosystems™

The Applied Biosystems® TaqMan® GAPDH Assay with JUN® dye-labeled QSY® probe (primer limited) provides a pre-formulated assay for quantitating human GAPDH. This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays. It consists of a JUN® dye-labeled QSY® probe plus sequence-specific forward and reverse primers. The assay can be used for multiplex or singleplex PCR reactions, and it is primer limited, so it can be used with samples that overexpress GAPDH.

Benefits of this assay include:

• Eliminate assay design time by using pre-designed primers and pre-designed JUN® dye-labeled TaqMan® probe
• Minimize experimental optimization time
• Use in conjunction with FAM™ or VIC® dye-labeled predesigned gene expression assays
• Formulated at a primer-limited concentration for use in multiplex reactions

Save time in assay design, formulation, and testing by using the TaqMan® GAPDH Assay as your control when quantifying gene expression. This pre-designed probe and primer set enables you to normalize the amount of sample RNA or DNA in a reaction when used with a control sample. Or use it for relative gene expression quantification in cDNA samples when used with other gene expression assays.

In order to facilitate multiplex experiments, the assay formulation is primer limited, allowing the assay to be used with high GAPDH expressors.

The JUN® dye is optimized for use with the 4th filter of our QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments. Please note that master mix containing ROX™ dye cannot be used with this assay (with JUN® dye) due to an overlap of spectra. TaqMan® Multiplex Master Mix with MUSTANG PURPLE® dye is recommended.

TaqMan™ Fast Cells-to-CT™ Kit Invitrogen™

The TaqMan® Fast Cells-to-CT™ Kit makes it possible to use Fast-cycling, real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification.

The TaqMan® Fast Cells-to-CT Kit is:

• Complete—optimized workflow includes cell lysis reagents with gDNA removal, RT enzyme mix, RT buffer, and TaqMan® Fast Universal PCR Master Mix
• Fast—prepare samples at room temperature in 7 minutes, including DNase treatment; TaqMan® Fast Universal PCR Master Mix delivers real-time PCR results in ~35 minutes
• Convenient—lyse samples in a tube or directly in 96- or 384-well culture plates
• Robust—perform gene expression analysis on 10 to 100,000 cells per sample, with results equivalent to those from purified RNA
• Efficient—contains sufficient reagents to generate 500 real-time PCR results from 100 starting samples

Complete, validated solution
TaqMan® Cells-to-CT™ technology features a unique method for lysing cultured cells while removing genomic DNA (gDNA) and preserving RNA integrity. Additionally, the kit contains reverse transcription (RT) reagents and TaqMan® Fast Universal PCR Master Mix for Fast real-time PCR analysis, and has been extensively tested and validated with gold-standard TaqMan® Gene Expression assays, sold separately. All kit components have been optimized to work together for consistent and reliable performance, removing the guesswork involved in assembling separate kits for sample preparation, reverse transcription, and Fast real-time PCR.

Fast, convenient workflow
The TaqMan® Fast Cells-to-CT™ Kit incorporates a simple 7-minute sample preparation procedure (see figure) for use with 10 to 100,000 cultured cells directly in 96- or 384-well culture plates or in tubes. Cells are washed in PBS and lysed for 5 minutes at room temperature; an optional DNase treatment can be performed concurrently. Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution. The kit greatly simplifies a traditionally time-consuming, labor intensive process and reduces it to 7 minutes.

The lysates are now ready for reverse transcription with the optimized RT system included in the kit, or they can be stored at -20°C for up to 5 months, for future analysis. The supplied TaqMan® Fast Universal PCR Master Mix delivers sensitive and reproducible results approximately 3 times faster than standard PCR reagents and instruments. The combination of fast and simple sample preparation, and Fast-cycling real-time PCR results (~35 minutes) enables more rapid quantitation of gene targets than previously available. The TaqMan® Fast Cells-to-CT™ Kit workflow reduces the time from sample to result by 40% or more, compared to traditional RNA purification and standard real-time PCR.

Robust, sensitive, and reliable results
Accurate gene expression analysis over a wide range of sample input for genes with various expression levels has traditionally been a challenge for researchers. Therefore, a robust solution is important for profiling RNA from a few or many cells. Shown is the demonstration of the excellent signal linearity (R2 = 0.997) observed using lysates from TaqMan® Fast Cells-to-CT™ Kit across 5 logs of cellular input (10–105 cells per lysis reaction) (see figure). Thus, a single kit can be used for analysis of gene expression from limited or dense samples, with no compromise in data integrity caused by PCR inhibitors.

PCR performance of cell lysates using the TaqMan® Fast Cells-to-CT™ Kit was equivalent to that from purified RNA, using the supplied TaqMan® Fast Universal PCR Master Mix (see figure). Both TaqMan® Cells-to-CT™ Kit lysates and purified RNA showed linear detection over a 5 log range of cell input equivalents. The enhanced sensitivity at the 10-cell input with TaqMan® Fast Cells-to-CT™ Kit lysates compared to purified RNA, using the same RT and PCR reagents, results from more efficient retention of target molecules during preparation, i.e., no loss of RNA typically associated with sample transfer or heating.

The TaqMan® Fast Cells-to-CT™ Kit also demonstrated superior performance compared to purified RNA which was reverse transcribed and PCR amplified with Competitor Q’s RT and Fast Master Mix. The observed dynamic range using Competitor Q’s reagents with Fast cycling was 102 to 105 cell equivalents, or 1 log less than TaqMan® Cells-to-CT™ Kit lysates or purified RNA amplified with TaqMan® Fast Universal PCR Master Mix (see figure). The TaqMan® Fast Universal PCR Master Mix is formulated to deliver sensitive and reproducible results faster than is possible with standard reagents and instruments, and is robust across a wide range of target genes and concentrations.

TaqPath™ 1-Step RT-qPCR Master Mix, CG Applied Biosystems™

TaqPath™ 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX™ dye, and thermostable Fast DNA polymerase, facilitating easy reaction set up—just add user-supplied assay and sample (Figure 1). All manufacturing lots are functionally tested to ensure remarkable lot-to-lot reproducibility for CT consistency and dynamic range across a wide variety of assays. TaqPath™ 1-Step RT-qPCR Master Mix, CG, is a General Purpose Reagent (GPR), manufactured according to applicable 21 CFR requirements and is labeled 'For Laboratory Use'. With stringent quality coupled with premier performance, TaqPath™ 1-Step RT-qPCR Master Mix, CG, is a superior choice for your diagnostic testing or development needs.

Features of the TaqPath™ 1-Step RT-qPCR Master Mix, CG, include:

• High sensitivity to detect low-copy targets with reproducible CT results
• 6 logs of dynamic range with RNA, DNA, and multiplexing applications*
• Tolerance of inhibitors commonly found in clinical samples
• Labeled 'For Laboratory Use'

High Sensitivity
With understanding of the importance of reproducible detection for low-titer pathogen or transcripts in clinical diagnostic testing, TaqPath™ 1-Step RT-qPCR Master Mix, CG, has been optimized as a higher-concentration 4X master mix that allows you to input more sample into each reaction, increasing sensitivity even in low-volume reactions. Figure 2 shows consistent CT results obtained from three unique lots when detecting 10 copy inputs of RNA target. Figure 3 demonstrates CT lot-to-lot consistency is preserved across multiple assays with different attributes and expression levels.

Wide Dynamic Range Compatible with RNA, DNA, and Multiplexing
TaqPath™ 1-Step RT-qPCR Master Mix, CG, has been optimized to provide high specificity and dynamic range for use with both RNA and DNA targets. Since virology labs often test for both RNA and DNA viruses, TaqPath™ 1-Step RT-qPCR Master Mix, CG, is designed to use a single protocol to assay both types of nucleic acid. This input flexibility can help streamline the number of different workflows in your lab to improve efficiency. Figure 4 demonstrates the excellent PCR linearity across a 6-log input range for both RNA (Figure 4a) and DNA targets (Figure 4b).

TaqPath™ 1-Step RT-qPCR Master, CG, is compatible with multiplexing reactions, allowing for additional exogenous or endogenous controls or targets to be run simultaneously for quality control or efficiency purposes. Figure 5 demonstrates TaqPath™ 1-Step RT-qPCR Master Mix performance in a triplex reaction. The formulation can be run in either fast or standard cycling conditions with equivalent performance across a wide variety of qPCR platforms.

TaqPath™ 1-Step RT-qPCR Master Mix, CG, has been benchmarked against similar competitor master mixes and demonstrates equivalent or better sensitivity and dynamic range across a variety of targets (Figure 6).

Inhibitor Tolerant
Unlike other master mixes on the market, TaqPath™ 1-Step RT-qPCR Master Mix’s unique proprietary formulation allows robust performance even in the presence of substances that can normally inhibit PCR, such as heparin, hematin, or EDTA, increasing your confidence when working with a variety of complex clinical samples. TaqPath™ master mixes demonstrate increased tolerance of inhibitors compared to competitor mixes (Figure 7).

Manufacturing Production and Process Controls
TaqPath™ qPCR Master Mix is manufactured under a strong quality management system that utilizes traceable quality raw materials and validated operating procedures. With established controls from purchasing through QC release, TaqPath™ product manufacturing is designed to produce consistent product performance lot after lot.

General Purpose Reagent
For customers in the USA, TaqPath™ 1-Step RT-qPCR Master Mix is a General Purpose Reagent and 'For Laboratory Use'.

ABsolute Blue QPCR Mix, low ROX Thermo Scientific™

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Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of ABsolute Blue QPCR Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific ABsolute Blue qPCR Mixes are 2X master mixes for probe chemistry. There are suitable alternatives available for all common real-time instruments. ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity. The master mixes incorporate an inert blue dye to enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

Highlights

High performance—ABsolute sensitivity, consistency & convenience
Enhanced contrast and reduced errors—easily identify correctly aliquotted reaction mix in all plates and tubes
Universal—compatible with all major qPCR platforms
Adaptable—available with different ROX passive reference dye concentrations

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

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High-Capacity cDNA Reverse Transcription Kit Applied Biosystems™

The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single-stranded cDNA in a single 20 µL reaction. The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

Features of this kit include:

• Linear target amplification for real-time PCR
• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
• 10-fold greater dynamic range than other kits

Extensively tested with a variety of templates
Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.

TaqMan™ RNase P Assay, ABY™ dye/QSY™ probe Applied Biosystems™

The Applied Biosystems® TaqMan® RNaseP Assay with ABY® dye-labeled QSY® probe provides a pre-formulated assay for quantitating human RNase P. This assay enables relative gene expression quantification in cDNA samples when used with other gene expression assays. It consists of an ABY® dye-labeled QSY® probe plus sequence-specific forward and reverse primers, and it can be used for multiplex or singleplex PCR reactions.

Benefits of this assay include:

• Eliminate assay design time using pre-designed primers and pre-designed ABY® dye-labeled TaqMan® probe
• Minimize experimental optimization time
• Use in conjunction with FAM™ or VIC® dye-labeled predesigned gene expression assays

Save time in assay design, formulation, and testing by using the TaqMan® RNase P Assay as your control when quantifying gene expression. This pre-designed probe and primer set enables you to normalize the amount of sample RNA or DNA in a reaction when used with a control sample. Or use it for relative gene expression quantification in cDNA samples when used with other gene expression assays.

The ABY® dye is optimized for use with the 3rd filter of our QuantStudio™, ViiA™ 7, and 7500 series real-time PCR instruments.

Megaplex™ RT Primers, Human Pool A v2.1 Applied Biosystems™

Designed to streamline experimental workflows, Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan® MicroRNA Array. Human Pool A v2.1 contains RT primers for 377 unique microRNAs and 4 controls. The microRNA targets represented in this pool tend be better characterized, more broadly expressed and⁄or expressed at a higher level compared to Human Pool B. Human Pool A v2.1 is intended for use with the TaqMan® Array, Human MicroRNA A Card v2.0, however, it can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

Custom TaqMan™ Escherichia coli O104:H4 Assay Beads Thermo Scientific™

These TaqMan® assay beads provide a simple, reliable, and rapid method for detecting the presence of a specific bacterial or viral target. The assay uses PCR to amplify a DNA target unique to the microorganism and TaqMan® probe to detect and quantify the target, all in an an easy-to-use, lyophilized bead format. TaqMan® assay beads deliver the fastest RT-PCR run times on the market (<40 minutes in Fast mode). Simply add 30 µL of purified DNA to the beads and run on Fast or standard instrumentation. These TaqMan® assay beads provide microbial detection capabilities and high-confidence results to help accelerate your food and environmental testing needs.

Features of TaqMan® assay beads:

• Convenience—simply add your nucleic acid sample to the bead-containing assay tube and run; no pre-mixing or master mix preparation required
• Consistency—fewer operator steps means reduced error and increased reproducibility across time, users, and laboratories
• Improved data accuracy—an internal positive control is included to monitor your PCR
• Fast chemistry—40-min run time with Fast cycling, compatible with standard or Fast modes

TaqMan® assay beads are suitable for single or multiplex analysis. Because they are run using universal conditions, multiple bead-based assays can be analyzed in the same run. The robust assay design provides highly accurate, reproducible, and reliable results, with the enhanced stability of a lyophilized format. A passive internal reference based on proprietary ROX™ dye is included for precise data analysis.

This product is for environmental testing, quality control/quality assurance testing, food and agricultural testing, and research purposes only.

Custom assays have been bioinformatically developed and tested for performance. Validation services for specific samples types are provided by our Custom Solutions team. Please contact your local Thermo Fisher Scientific representative for more information.

NGS Reverse Transcription Kit Ion Torrent™

The Ion Torrent NGS Reverse Transcription (RT) Kit is the first cDNA synthesis kit developed specifically for next-generation sequencing (NGS) applications. It combines the superior performance of SuperScript IV Reverse Transcriptase with a novel master mix optimized for NGS library preparation. The NGS RT Kit is compatible with Ion AmpliSeq and Ion AmpliSeq HD assays and can be used with both manual and automated workflows.

Benefits of the NGS RT Kit include:
• Optimized for a variety of sample types, including FFPE, degraded samples, and those with low template amounts
• Superior NGS results with novel RT formulation optimized for NGS library preparation
• Helps increase NGS library yield, improve detection of long amplicons, and rescue under-performing amplicons
• Helps maximize mapped reads and improve detection of RNA fusions
• Trusted SuperScript IV RT technology offers superior cDNA synthesis performance with even the most challenging RNA samples
• Universal kit for wide variety of Oncomine oncology research assays and Ion AmpliSeq panels

Simple reaction setup
The NGS RT Kit contains a tube of 10X SuperScript IV enzyme mix and a tube of 5X buffer. A one-step, 25-minute reaction reverse transcribes total RNA into cDNA and can be used directly for NGS library preparation, without any additional quantification, purification, or dilution steps. A variety of sample types can be used, including RNA from blood, bone marrow, FFPE and partially degraded samples. This product is compatible with Oncomine and Ion AmpliSeq workflows, as well as Ion AmpliSeq HD panels. Consult the cDNA synthesis section of each assay manual for specific RT reaction volumes and setup instructions.

Performance and quality testing
The NGS RT Kit is assayed for endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease, as well as yield and length of cDNA product. It is QC tested with a full NGS workflow to evaluate library yield and ensure performance meets key sequencing metrics.

VetMAX™ C. burnetii & Chlamydophila spp. Kit Thermo Scientific™

Note: The name of this product has changed. It was previously called LSI VetMAX LSI VetMAX™ Triplex Coxiella burnetii & Chlamydophila spp..

Same as TaqVet Triplex Coxiella burnetii & Chlamydophila spp. Real-Time PCR Kit (TFQQCHP-CY5)

TaqMan® probes - DNA - triplex - One well reaction

Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals.

The LSI VetMAX™ kits include:

• 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for RNA kits).
• 1 external positive control of the same nature as the target (inactivated bacteria or virus). Use as a quality control for PCR and/or extraction yield.
• 1 internal positive control (exogenous) to be added at the time of extraction. Use as a quality control for extraction yield.
• A quality control certificate.

For veterinary use only.

Regulatory requirements vary by country; products may not be available in your geographic area.

Biological hazard safety

WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. All work should be conducted in properly equipped facilities using the appropriate safety equipment (for example, physical containment devices). Safety equipment also may include items for personal protection, such as gloves, coats, gowns, shoe covers, boots, respirators, face shields, safety glasses, or goggles. Individuals should be trained according to applicable regulatory and company/institution requirements before working with potentially biohazardous materials. Follow all applicable local, state/provincial, and/or national regulations. The following references provide general guidelines when handling biological samples in laboratory environment.

U.S. Department of Health and Human Services, Biosafety in Microbiological and Biomedical Laboratories (BMBL), 5th Edition, HHS Publication No. (CDC) 21-1112, Revised December 2009

World Health Organization, Laboratory Biosafety Manual, 3rd Edition, WHO/CDS/CSR/LYO/2004.11

TaqMan™ Protein Assays Core Reagents Kit with MasterMix Applied Biosystems™

The TaqMan® Protein Assays Core Reagents Kit with MasterMix contains both the Core Reagents Base Kit and the TaqMan Protein Assays Fast Master Mix Kit. The Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan® protein expression experiments. The Universal PCR Assay contains optimized concentrations of TaqMan® probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems real-time PCR system. The enzyme concentration, ionic strength, and the pH of the TaqMan® Protein Expression Fast Master Mix are optimized for the amplification of ligated oligonucleotide target sequences prepared using TaqMan® Protein Expression Assay reagents. The DNA polymerase included in the master mix is designed for maximum PCR efficiency and specificity when used with the ligation chemistry.
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