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TaqMan™ Gene Expression Cells-to-CT™ Kit Invitrogen™

The TaqMan® Gene Expression Cells-to-CT™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification. This kit saves time and offers a simple workflow that is suitable for a few samples or can be easily incorporated into automated, high-throughput applications.

The TaqMan® Gene Expression Cells-to-CT Kit is:

• Complete—optimized workflow includes cell lysis reagents with gDNA removal, RT enzyme mix, buffer, and new TaqMan® Gene Expression Master Mix
• Fast—7-minute sample prep, including DNase treatment, at room temperature
• Easy—lyse samples in a tube or directly in culture plates
• Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
• Efficient—contains sufficient reagents to generate 500 real-time PCR results from 100 starting samples

Featuring a unique method for lysing cultured cells while removing genomic DNA and preserving RNA integrity, the TaqMan® Gene Expression Cells-to-CT™ Kit contains reverse transcription (RT) reagents for cDNA synthesis and TaqMan® Gene Expression Master Mix for real-time PCR analysis. TaqMan® primer⁄probe sets are sold separately.

Simple 7-minute sample preparation: part of a complete workflow
Whether you are using plates or tubes, the TaqMan® Gene Expression Cells-to-CT™ Kit, which uses the simple 7-minute sample preparation procedure outlined (see figure), is designed for 10–100,000 cultured cells⁄sample. Cells are washed in PBS and lysed in solution for 5 minutes at room temperature; DNase treatment can be performed simultaneously. Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution. The lysates are now ready for reverse transcription or storage at –20°C. Because samples can be processed directly in culture wells (96 or 384 wells), sample handling and the potential for sample loss or transfer error are minimized, facilitating rapid, high-throughput processing. Unlike old-fashioned, multi-step RNA isolation protocols, no heating, washing, or centrifugation steps are required. The kit greatly simplifies a laborious 30–60 minute process and reduces it to 7 minutes.

The TaqMan® Gene Expression Cells-to-CT™ Kit workflow enables unsurpassed gene expression evaluation with any of the >700,000 TaqMan® Gene Expression Assays. This kit has been extensively tested for specificity with a broad selection of TaqMan® Gene Expression Assays and shows performance equivalent to that obtained with purified RNA (see figure).

Achieve unsurpassed performance and sensitivity
Unlike some competitor kits that limit the amount of lysate in the RT reaction to 5%, the TaqMan® Gene Expression Cells-to-CT™ Kit can accommodate 45% of the total RT reaction volume as cell lysate. Additionally, cDNA can comprise up to 45% of the real-time PCR reaction volume. The large lysate volume in the optimized RT reaction, along with the large cDNA volume in the subsequent real-time PCR using the TaqMan® Gene Expression Master Mix, lead to maximum sensitivity. The master mix amplifies the target precisely and accurately, enabling the detection of small quantities of target, such as transcripts expressed at low levels.

The ability to detect limited target quantities was tested by mixing a constant number of human cells with various numbers of mouse cells. The cell mixtures were prepared using the TaqMan® Gene Expression Cells-to-CT™ and assayed for a mouse-specific and human-specific gene. Comparative data were generated in the same manner with RNA purified by traditional methodology. The data show that RNA from as few as 10 mouse cells was detectable in a background of RNA from 10,000 human cells (see figure). The ability of the TaqMan® Gene Expression Cells-to-CT™ Kit to detect relative low abundance transcripts was equivalent to that of purified RNA; at low levels, it was superior.

Additionally, the performance of the TaqMan® Gene Expression Cells-to-CT™ Kit was compared to competitor lysate kits and to purified RNA. Inputs of 100–100,000 cells⁄lysis reaction were examined. The sensitivity of the TaqMan® Cells-to-CT™ Kit protocol was equivalent to that obtained with purified RNA, and it surpassed competitor lysates (see figure). Furthermore, the lack of inhibition at high cellular inputs and the low variation among technical replicates demonstrate the reliability of this approach for gene expression studies using cultured cells.

Proven performance, proven together
All components of the TaqMan® Gene Expression Cells-to-CT™ Kit have been optimized for consistent and reliable performance. This removes the guesswork involved in assembling separate sample preparation, RT, and real-time PCR kits. And the TaqMan® Gene Expression Cells-to-CT™ Kit has been validated with TaqMan® Gene Expression Assays for added quality assurance.

Accessory product
The TaqMan® Cells-to-CT™ Control Kit was designed for use with the TaqMan® Gene Expression Cells-to-CT™ Kit. This kit contains an endogenous control (ACTB) to normalize for sample loading variability and an artifical XenoRNA™ control and corresponding TaqMan® Gene Expression Assay to monitor the effects of PCR inhibition.

qualyfast™ Legionella PCR and DNA Extraction Kits Thermo Scientific™

Detect both Legionella species and Legionella pneumophila from water samples with this rapid, sensitive and specific solution enabled by the qualyfast® Legionella PCR Assay, Free DNA Inactivator, DNA Extraction and Quantification Standards kits.The assay independently detects both Legionella species and L. pneumophilia, includes inhibition control to minimize false-negative results, and offers the option for quantification by using calibrated standards. All consumables required for the workflow are included within the kits, with lyophilized and pre-dosed PCR reagents for ease-of-use.

TaqMan™ Fast Cells-to-CT™ Kit Invitrogen™

The TaqMan® Fast Cells-to-CT™ Kit makes it possible to use Fast-cycling, real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification.

The TaqMan® Fast Cells-to-CT Kit is:

• Complete—optimized workflow includes cell lysis reagents with gDNA removal, RT enzyme mix, RT buffer, and TaqMan® Fast Universal PCR Master Mix
• Fast—prepare samples at room temperature in 7 minutes, including DNase treatment; TaqMan® Fast Universal PCR Master Mix delivers real-time PCR results in ~35 minutes
• Convenient—lyse samples in a tube or directly in 96- or 384-well culture plates
• Robust—perform gene expression analysis on 10 to 100,000 cells per sample, with results equivalent to those from purified RNA
• Efficient—contains sufficient reagents to generate 500 real-time PCR results from 100 starting samples

Complete, validated solution
TaqMan® Cells-to-CT™ technology features a unique method for lysing cultured cells while removing genomic DNA (gDNA) and preserving RNA integrity. Additionally, the kit contains reverse transcription (RT) reagents and TaqMan® Fast Universal PCR Master Mix for Fast real-time PCR analysis, and has been extensively tested and validated with gold-standard TaqMan® Gene Expression assays, sold separately. All kit components have been optimized to work together for consistent and reliable performance, removing the guesswork involved in assembling separate kits for sample preparation, reverse transcription, and Fast real-time PCR.

Fast, convenient workflow
The TaqMan® Fast Cells-to-CT™ Kit incorporates a simple 7-minute sample preparation procedure (see figure) for use with 10 to 100,000 cultured cells directly in 96- or 384-well culture plates or in tubes. Cells are washed in PBS and lysed for 5 minutes at room temperature; an optional DNase treatment can be performed concurrently. Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution. The kit greatly simplifies a traditionally time-consuming, labor intensive process and reduces it to 7 minutes.

The lysates are now ready for reverse transcription with the optimized RT system included in the kit, or they can be stored at -20°C for up to 5 months, for future analysis. The supplied TaqMan® Fast Universal PCR Master Mix delivers sensitive and reproducible results approximately 3 times faster than standard PCR reagents and instruments. The combination of fast and simple sample preparation, and Fast-cycling real-time PCR results (~35 minutes) enables more rapid quantitation of gene targets than previously available. The TaqMan® Fast Cells-to-CT™ Kit workflow reduces the time from sample to result by 40% or more, compared to traditional RNA purification and standard real-time PCR.

Robust, sensitive, and reliable results
Accurate gene expression analysis over a wide range of sample input for genes with various expression levels has traditionally been a challenge for researchers. Therefore, a robust solution is important for profiling RNA from a few or many cells. Shown is the demonstration of the excellent signal linearity (R2 = 0.997) observed using lysates from TaqMan® Fast Cells-to-CT™ Kit across 5 logs of cellular input (10–105 cells per lysis reaction) (see figure). Thus, a single kit can be used for analysis of gene expression from limited or dense samples, with no compromise in data integrity caused by PCR inhibitors.

PCR performance of cell lysates using the TaqMan® Fast Cells-to-CT™ Kit was equivalent to that from purified RNA, using the supplied TaqMan® Fast Universal PCR Master Mix (see figure). Both TaqMan® Cells-to-CT™ Kit lysates and purified RNA showed linear detection over a 5 log range of cell input equivalents. The enhanced sensitivity at the 10-cell input with TaqMan® Fast Cells-to-CT™ Kit lysates compared to purified RNA, using the same RT and PCR reagents, results from more efficient retention of target molecules during preparation, i.e., no loss of RNA typically associated with sample transfer or heating.

The TaqMan® Fast Cells-to-CT™ Kit also demonstrated superior performance compared to purified RNA which was reverse transcribed and PCR amplified with Competitor Q’s RT and Fast Master Mix. The observed dynamic range using Competitor Q’s reagents with Fast cycling was 102 to 105 cell equivalents, or 1 log less than TaqMan® Cells-to-CT™ Kit lysates or purified RNA amplified with TaqMan® Fast Universal PCR Master Mix (see figure). The TaqMan® Fast Universal PCR Master Mix is formulated to deliver sensitive and reproducible results faster than is possible with standard reagents and instruments, and is robust across a wide range of target genes and concentrations.

Megaplex™ RT Primers, Human Pool B v3.0 Applied Biosystems™

Designed to streamline experimental workflows, Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan® MicroRNA Array. Human Pool B v3.0 contains RT primers for 377 unique microRNAs and 4 controls. The microRNA targets represented in this pool tend be less well characterized, more narrowly expressed and⁄or expressed at a lower level compared to Human Pool A. Human Pool B v3.0 is intended for use with the TaqMan® Array Human MicroRNA B Card v3.0, however, it can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

VetMAX™ BTV NS3 All Genotypes Kit Thermo Scientific™

Note: The name of this product has changed. It was previously called LSI VetMAX Bluetongue Virus NS3 - All genotypes.

Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals.

The LSI VetMAX kits include:

• 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan real-time PCR master mix + an RT enzyme (only for RNA kits)
• 1 external positive control of the same nature as the target (inactivated bacteria or virus). Use as a quality control for PCR and/or extraction yield.
• 1 internal positive control (exogenous) to be added at the time of extraction. Use as a quality control for extraction yield.
• A quality control certificate

Regulatory requirements vary by country; products may not be available in your geographic area.

Biological hazard safety
WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. All work should be conducted in properly equipped facilities using the appropriate safety equipment (for example, physical containment devices). Safety equipment also may include items for personal protection, such as gloves, coats, gowns, shoe covers, boots, respirators, face shields, safety glasses, or goggles. Individuals should be trained according to applicable regulatory and company/institution requirements before working with potentially biohazardous materials. Follow all applicable local, state/provincial, and/or national regulations. The following references provide general guidelines when handling biological samples in laboratory environment.
U.S. Department of Health and Human Services, Biosafety in Microbiological and Biomedical Laboratories (BMBL), 5th Edition, HHS Publication No. (CDC) 21-1112, Revised December 2009
World Health Organization, Laboratory Biosafety Manual, 3rd Edition, WHO/CDS/CSR/LYO/2004.11

Megaplex™ RT Primers, Rodent Pool A Applied Biosystems™

Designed to streamline experimental workflows, Applied Biosystems® Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan® MicroRNA Array. Rodent Pool A contains RT primers for 335 and 238 unique microRNAs for mouse and rat respectively plus 4 species specific controls. The microRNA targets represented in this pool tend be better characterized, more broadly expressed and⁄or expressed at a higher level compared to Human Pool A. The pool can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

Megaplex™ PreAmp Primers, Rodent Pool A Applied Biosystems™

For use when assay sensitivity is of the utmost importance, Applied Biosystems® Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. With content matched to the Megaplex™ RT Primers, Megaplex™ PreAmp Primers, Rodent Pool A is designed to be used with Megaplex™ RT Primers, Rodent Pool A. Following reverse transcription using Megaplex™ RT Primers, Megaplex™ PreAmp Primers along with the TaqMan® PreAmp Master Mix uniformly amplify all microRNAs prior to quantitation by real-time PCR using TaqMan® MicroRNA Arrays.

VetMAX™-Gold BVDV PI Detection Kit Applied Biosystems™

The Bovine Virus Diarrhea RNA Test Kit (VetMAX™-Gold BVDV PI Detection Kit) is intended for use in the rapid, in vitro detection of bovine virus diarrhea virus (BVDV RNA) extracted from ear notches in acute and persistently infected (PI) cattle.

The VetMAX™-Gold BVDV PI Detection Kit is the first USDA-licensed real-time PCR test for the detection of BVDV in cattle. The assay is a single-tube, real-time, reverse transcription-polymerase chain reaction (RT-PCR) in which RNA is reverse transcribed into cDNA, and BVDV targets are amplified and detected in real time using fluorescent TaqMan® probes.

Have Confidence in Your Results:
• Proven repeatability and reproducibility in field studies
• Highly specific and sensitive detection of type 1 and type 2 BVDV
• Consistently detects <40 RNA copies
• Contains Xeno™ RNA internal positive control for confirmation of results
• Rapid and cost-effective method for detection of BVDV with results in ~1.5 hours
• Simple, fast workflow minimizes sample handling and reduces sample cross-contamination
• Easily automated for high-throughput processing
• Detects BVDV in ear-notch tissue
• Robust and reliable AmpliTaq Gold® DNA Polymerase and TaqMan® technology

Limitations:
• The kit is not intended for discriminating viral subtypes
• Samples should be handled as recommended to prevent degradation of any BVDV RNA that is present
• RNA extraction methods should yield RNA free of RT-PCR inhibitors, which can prevent amplification of target RNA
• Follow “good PCR practices" to prevent false positive amplifications due to contamination of test samples with PCR products

Megaplex™ PreAmp Primers, Rodent Pool Set v3.0 Applied Biosystems™

For use when assay sensitivity is of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. With content matched to the Megaplex™ RT Primers, Megaplex™ PreAmp Primers, Rodent Pools Set v3.0 is used with Megaplex™ RT Primers, Rodent Pools Set v3.0. Following reverse transcription using Megaplex™ RT Primers, Megaplex™ PreAmp Primers along with the TaqMan® PreAmp Master Mix uniformly amplify all microRNAs prior to quantitation by real-time PCR using TaqMan® MicroRNA Arrays.

TaqMan® Mutation Detection Assay with castPCR™ Technology Applied Biosystems™

Applied Biosystems TaqMan Mutation Detection Assays are powered by competitive allele-specific TaqMan castPCR technology to detect and measure somatic mutations in genes associated with cancer research. castPCR technology is highly specific and sensitive, and can detect rare amounts of mutated DNA in a sample that contains large amounts of normal, wild-type DNA. These assays are compatible with different sample types, such as cell lines, formalin-fixed paraffin-embedded (FFPE) tissue samples, and fresh-frozen tissue samples.

castPCR technology
Competitive, allele-specific TaqMan PCR utilizes an allele-specific primer for mutant allele detection that competes with an MGB blocker oligonucleotide to suppress the wild-type background.

Somatic mutation detection
By combining the power of TaqMan and castPCR technologies, TaqMan Mutation Detection Assays provide:

Higher specificity—designed to block the wild-type and amplify only the mutant
Higher sensitivity—designed to detect somatic mutations down to 1 cancer cell in 1,000 normal cells
Faster workflow—3 hours from sample to result

With 819 assays for 47 known cancer research genes available today like KRAS, BRAF, KIT, JAK2, the TaqMan Mutation Detection Assays provide an innovative tool for your cancer research.

All TaqMan Mutation Detection Assays have undergone extensive testing to ensure verify high sensitivity and specificity.

Custom TaqMan™ Campylobacter coli Assay Thermo Scientific™

This Taqman® assay provides a simple, reliable, and rapid procedure for detecting the presence of a specific bacterial or viral target. PCR is used to amplify a DNA target unique to the microorganism, then TaqMan® probe is used to detect and quantify the target in a easy-to-use, single tube format. This assay enables microbial detection capabilities with high-confidence results to will help accelerate your food and environmental testing needs. It has been optimized for use with the included Environmental Master Mix; a negative control is not included with this assay.

Features of TaqMan® assays:

• Include an internal positive control to monitor your PCR
• Suitable for single or multiplexed assays
• Easy-to-use, single tube format
• Run using universal conditions- analyze multiple assays in the same run
• Robust assay design provides highly accurate, reproducible, and reliable results
• Include passive internal reference based on proprietary ROX™ dye for precise data analysis

This product is for environmental testing, quality control/quality assurance testing, food and agricultural testing, and research purposes only.

Custom assays have been bioinformatically developed and tested for performance. Validation services for specific samples types are provided by our Custom Solutions team. Please contact your local Thermo Fisher Scientific representative for more information.

Megaplex™ RT Primers, Rodent Pool B v3.0 Applied Biosystems™

Designed to streamline experimental workflows, Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan® Array MicroRNA Card. Rodent Pool B v2.0 contains RT primers for 306 and 135 unique microRNAs for mouse and rat respectively plus 4 species specific controls. The microRNA targets represented in this pool tend be less well characterized, more narrowly expressed and⁄or expressed at a lower level when compared to Rodent Pool A. The pool can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

Luminaris Color HiGreen qPCR Master Mix, low ROX Thermo Scientific™

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris Color HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

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Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1). The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• Blue master mix and Yellow Sample Buffer for easy pipetting
• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: single copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix


Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris Color HiGreen Low ROX qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer with a blue dye. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with 40X Yellow Sample Buffer and nuclease-free water.

MeltDoctor™ HRM Reagent Kit Applied Biosystems™

Applied Biosystems® MeltDoctor™ HRM Reagent Kit provides individually packaged PCR components and the MeltDoctor™ HRM Dye required for high resolution melting analysis. High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences.
This kit includes:
• AmpliTaq Gold® 360 DNA Polymerase which, when combined with AmpliTaq Gold® 360 Buffer and 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold® 360 DNA Polymerase delivers 360° coverage for a full range of targets.
• GeneAmp® dNTP Blend
• MeltDoctor™ HRM Dye, a stabilized form of the fluorescent SYTO® 9 double-stranded nucleic acid stain developed by Molecular Probes

Luminaris HiGreen qPCR Master Mix, fluorescein Thermo Scientific™

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: single copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix

Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris HiGreen Fluorescein qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains fluorescein passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied nuclease-free water.
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