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80 30 17 40

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800 872 987

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00800 5345 5345

Netherlands

0800 099 3310

Portugal

800 206 639

Spain

900 181 461

Sweden

020 26 34 52

Switzerland

0800 84 88 00

United Kingdom

0800 269 210

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Australia

1300 735 292

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800 820 8982

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86 800 820 8982

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86 800 820 8982

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852 2407 8450

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81 3 6832 6980

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0800 933 966

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65 6861 8638

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886 2 2358 2838

Shop All Real Time PCR Reagents and Kits

Activation Time


Compatibility


Labeling Target


Overhang


Promoter


Sample Type


resDNASEQ™ Quantitative Vero DNA Kit with PrepSEQ™ Residual DNA Sample Preparation Kit Applied Biosystems™

The resDNASEQ Quantitative Vero DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Vero (African Green Monkey) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of Vero cell DNA, typically in less than four hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high molecular weight or sheared DNA.

This kit is bundled together with sample preparation reagents in the form of a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts. For the quantitation kit alone without the sample preparation kit, please see resDNASEQ Quantitative Vero DNA Kit.

• Highly sensitive quantitation using proven TaqMan real-time qPCR technology
• Offers consistent performance across the expected range of DNA fragment sizes
• Integrated system includes sample preparation kit, master mix, TaqMan primer/probe mix, and Vero genomic DNA standard

The resDNASEQ Quantitative Vero DNA Kit is designed to provide highly sensitive detection of Vero DNA, allowing you to use small sample volumes to help generate accurate results. The broad linear range of TaqMan real-time qPCR technology allows you to test samples with variable levels of Vero DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts.

Easy to use, results in under 4 hours
The easy workflow of the resDNASEQ Quantitative Vero DNA Kit consists of sample preparation, assay setup, and instrument run and data analysis—all of which you can perform typically in under 4 hours. Start by extracting DNA from your in-process purification and drug substance samples. Then perform qPCR analysis to compare the amount of Vero DNA in your test samples to a standard curve generated with known amounts of purified Vero genomic DNA standard. The resDNASEQ system includes almost everything you need to carry out these steps, from sample preparation kit to master mix to TaqMan primer/probe mix to Vero genomic DNA standard, as well as step-by-step instructions.

Consistent performance even with fragmented DNA
For accurate quantitation of residual Vero DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ system you get consistent assay performance across a range of DNA fragment sizes.

VetMAX™ Ruminant Respiratory Screening Kit Thermo Scientific™

Note: The name of this product has changed. It was previously called LSI VetMAX Screening Pack - Ruminant Respiratory Pathogens.

Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals.

The LSI VetMAX™ kits include:

• 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for RNA kits)
• 1 external positive control of the same nature as the target (inactivated bacteria or virus). Use as a quality control for PCR and/or extraction yield.
• 1 internal positive control (exogenous) to be added at the time of extraction. Use as a quality control for extraction yield.
• A quality control certificate

For veterinary use only.

Regulatory requirements vary by country; products may not be available in your geographic area.

WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. All work should be conducted in properly equipped facilities using the appropriate safety equipment (for example, physical containment devices). Safety equipment also may include items for personal protection, such as gloves, coats, gowns, shoe covers, boots, respirators, face shields, safety glasses, or goggles. Individuals should be trained according to applicable regulatory and company/institution requirements before working with potentially biohazardous materials. Follow all applicable local, state/provincial, and/or national regulations. The following references provide general guidelines when handling biological samples in laboratory environment.

1. U.S. Department of Health and Human Services, Biosafety in Microbiological and Biomedical Laboratories (BMBL), 5th Edition, HHS Publication No. (CDC) 21-1112, Revised December 2009; found at: www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf.

2. World Health Organization, Laboratory Biosafety Manual, 3rd Edition, WHO/CDS/CSR/LYO/2004.11; found at: www.who.int/csr/resources/publications/biosafety/Biosafety7.pdf.

SureTect™ Salmonella Species PCR Assay Thermo Scientific™

Detect Salmonella species  in food and environmental samples with the Thermo Scientific™ SureTect™ Salmonella Species PCR Assay which incorporates optimized primer and probe components for highly-specific detection of Salmonella species. The assay has been AOAC-RI and NF VALIDATION™ validated with a variety of matrices and a range of samples sizes and enrichments, following the AOAC or NF validated methods, to reduce the need for in-house validation prior to use. The internal amplification control confirms correct assay conditions have occurred and results can be relied on.

TaqMan™ OpenArray™ RT PCR, Custom Format 56 QuantStudio™ 12K Flex Applied Biosystems™

Design your own plate, building from custom, inventoried, and pre-designed TaqMan® Gene expression assays. The OpenArray® Real-Time PCR System workflow enables one person to validate or screen up to 576 samples in one day without the use of robotics.

TaqMan™ Array, Format 48 Applied Biosystems™

The Custom TaqMan® Array Card is a 384-well microfluidic card designed to perform 384 simultaneous real-time PCR reactions without using liquid-handling robots or multichannel pipettors. Select target assays from thousands of inventoried TaqMan® Gene Expression Assays.

Luminaris HiGreen qPCR Master Mix, high ROX Thermo Scientific™

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: ingle copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix


Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris HiGreen High ROX qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains high concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with nuclease-free water.

SureTect™ Listeria Species PCR Assay Thermo Scientific™

Detect Listeria species simply, reliably and rapidly in even the most challenging food types with the Thermo Scientific™ SureTect™ Listeria Species PCR Assay which incorporates optimized primer and probe components for highly-specific detection of Listeria species. The internal amplification control confirms correct assay conditions have occurred and results can be relied on. The assay is AOAC RI and NF VALIDATION™ validated by AFNOR Certification for all human foods and environmental production samples.

TaqMan™ OpenArray™ RT PCR, Custom Format 112 QuantStudio™ 12K Flex Applied Biosystems™

Design your own plate, building from custom, inventoried, and pre-designed TaqMan® Gene expression assays. The OpenArray® Real-Time PCR System workflow enables one person to validate or screen up to 576 samples in one day without the use of robotics.

VetMAX™ PRRSV EU & NA 2.0 Kit Applied Biosystems™

The VetMAX PRRSV EU & NA 2.0 Kit is a ready-to-use, single-well real-time RT-PCR kit that enables reliable, rapid, and accurate detection of porcine reproductive and respiratory syndrome virus (PRRSV) strains in porcine samples. The European (EU) and North American (NA) strains are detected and differentiated using fluorescent hydrolysis probe chemistry. An exogenous internal positive control (IPC) is included.

The advanced technology of the VetMAX PRRSV EU & NA 2.0 Kit offers several benefits:
Highly accurate―updated design detects the four subtypes of the EU PRRSV Type 1 and NA PRRSV strains, including the HP-PRRSV strain
Fast―results obtained in less than two hours
Flexible―fast and standard amplification modes
Versatile―compatible with a variety of sample matrices, including serum, whole blood, semen, tissue, oral fluid, and cell culture supernatant samples in pools of up to five samples (serum, whole blood, and semen)

The kit offers the convenience of a simple and fast workflow combining the MagMAX CORE nucleic acid purification method with a fast PCR amplification mode for a result in less than two hours.

More than 780 field samples from 13 origins identified as positive or negative for PRRS virus by partner laboratories have been tested. The results showed 99.5% sensitivity and 99.6% specificity.

SuperScript™ III First-Strand Synthesis System Invitrogen™

The SuperScript® III First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system. The amount of starting material can vary from 1 pg–5 µg of total RNA. SuperScript® III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42–55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript® III RT is not significantly inhibited by ribosomal and transfer RNA, it may be used to synthesize first-strand cDNA from a total RNA preparation.

Using the SuperScript® III First-Strand System
cDNA synthesis is performed in the first step using either total RNA or poly(A)+-selected RNA primed with oligo(dT), random primers, or a gene-specific primer. In the second step, PCR is performed in a separate tube using primers specific for the gene of interest. For the PCR reaction, we recommend one of the following DNA polymerases: Platinum® Taq DNA Polymerase provides automatic hot-start conditions for increased specificity up to 4 kb, Platinum® Taq DNA Polymerase High Fidelity provides increased yield and high fidelity for targets up to 15 kb, and Platinum® Pfx DNA Polymerase provides maximum fidelity for targets up to 12 kb.

TaqMan™ SARS-CoV-2, Flu A/B, RSV RNA Control Applied Biosystems

The TaqMan SARS-CoV-2, Flu A/B, RSV RNA Control contains targets specific to the SARS-CoV-2, influenza A/B, and RSV genomic regions targeted by the TaqMan SARS-CoV-2, FluA/B RSV RT-PCR Assay Kit. It is recommended for use with this assay as a positive control to ensure reaction setup and reagent integrity when interpreting test results.

Luminaris HiGreen qPCR Master Mix, low ROX Thermo Scientific™

**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Luminaris HiGreen qPCR Master Mix and added additional capabilities for your gene expression analysis.

**********************
Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR. The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

The Hot Start Taq DNA polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control (see Reference 1).

The use of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms (see Real-time PCR instrument compatibility chart).

Highlights

• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance:
Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: single copy detection
Wide linear range: accurate quantification across 9 orders of magnitude
Reproducibility and convenience: ready-to-use 2X master mix


Applications

• Gene expression analysis
• siRNA validation
• Genotyping
• Pathogen detection

Includes

Luminaris HiGreen Low ROX qPCR Master Mix (2X) includes Hot Start Taq DNA Polymerase, UDG and dNTPs (also dUTP) in an optimized PCR buffer. The master mix contains low concentration of ROX passive reference dye for specific real-time platforms (see Real-Time PCR instrument compatibility chart) and is supplied with nuclease-free water.

SureTect™ Listeria monocytogenes PCR Assay Thermo Scientific™

Detect Listeria monocytogenes in even the most challenging food types with the Thermo Scientific™ SureTect™ Listeria monocytogenes PCR Assay which incorporates optimized primer and probe components for highly-specific detection of L. monocytogenes. The internal amplification control confirms correct assay conditions have occurred and results can be relied on. The assay is AOAC RI and NF certified for all human foods and environmental samples for additional confidence in your results.

TaqMan™ Fast Cells-to-CT™ Kit Invitrogen™

The TaqMan® Fast Cells-to-CT™ Kit makes it possible to use Fast-cycling, real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification.

The TaqMan® Fast Cells-to-CT Kit is:

• Complete—optimized workflow includes cell lysis reagents with gDNA removal, RT enzyme mix, RT buffer, and TaqMan® Fast Universal PCR Master Mix
• Fast—prepare samples at room temperature in 7 minutes, including DNase treatment; TaqMan® Fast Universal PCR Master Mix delivers real-time PCR results in ~35 minutes
• Convenient—lyse samples in a tube or directly in 96- or 384-well culture plates
• Robust—perform gene expression analysis on 10 to 100,000 cells per sample, with results equivalent to those from purified RNA
• Efficient—contains sufficient reagents to generate 500 real-time PCR results from 100 starting samples

Complete, validated solution
TaqMan® Cells-to-CT™ technology features a unique method for lysing cultured cells while removing genomic DNA (gDNA) and preserving RNA integrity. Additionally, the kit contains reverse transcription (RT) reagents and TaqMan® Fast Universal PCR Master Mix for Fast real-time PCR analysis, and has been extensively tested and validated with gold-standard TaqMan® Gene Expression assays, sold separately. All kit components have been optimized to work together for consistent and reliable performance, removing the guesswork involved in assembling separate kits for sample preparation, reverse transcription, and Fast real-time PCR.

Fast, convenient workflow
The TaqMan® Fast Cells-to-CT™ Kit incorporates a simple 7-minute sample preparation procedure (see figure) for use with 10 to 100,000 cultured cells directly in 96- or 384-well culture plates or in tubes. Cells are washed in PBS and lysed for 5 minutes at room temperature; an optional DNase treatment can be performed concurrently. Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution. The kit greatly simplifies a traditionally time-consuming, labor intensive process and reduces it to 7 minutes.

The lysates are now ready for reverse transcription with the optimized RT system included in the kit, or they can be stored at -20°C for up to 5 months, for future analysis. The supplied TaqMan® Fast Universal PCR Master Mix delivers sensitive and reproducible results approximately 3 times faster than standard PCR reagents and instruments. The combination of fast and simple sample preparation, and Fast-cycling real-time PCR results (~35 minutes) enables more rapid quantitation of gene targets than previously available. The TaqMan® Fast Cells-to-CT™ Kit workflow reduces the time from sample to result by 40% or more, compared to traditional RNA purification and standard real-time PCR.

Robust, sensitive, and reliable results
Accurate gene expression analysis over a wide range of sample input for genes with various expression levels has traditionally been a challenge for researchers. Therefore, a robust solution is important for profiling RNA from a few or many cells. Shown is the demonstration of the excellent signal linearity (R2 = 0.997) observed using lysates from TaqMan® Fast Cells-to-CT™ Kit across 5 logs of cellular input (10–105 cells per lysis reaction) (see figure). Thus, a single kit can be used for analysis of gene expression from limited or dense samples, with no compromise in data integrity caused by PCR inhibitors.

PCR performance of cell lysates using the TaqMan® Fast Cells-to-CT™ Kit was equivalent to that from purified RNA, using the supplied TaqMan® Fast Universal PCR Master Mix (see figure). Both TaqMan® Cells-to-CT™ Kit lysates and purified RNA showed linear detection over a 5 log range of cell input equivalents. The enhanced sensitivity at the 10-cell input with TaqMan® Fast Cells-to-CT™ Kit lysates compared to purified RNA, using the same RT and PCR reagents, results from more efficient retention of target molecules during preparation, i.e., no loss of RNA typically associated with sample transfer or heating.

The TaqMan® Fast Cells-to-CT™ Kit also demonstrated superior performance compared to purified RNA which was reverse transcribed and PCR amplified with Competitor Q’s RT and Fast Master Mix. The observed dynamic range using Competitor Q’s reagents with Fast cycling was 102 to 105 cell equivalents, or 1 log less than TaqMan® Cells-to-CT™ Kit lysates or purified RNA amplified with TaqMan® Fast Universal PCR Master Mix (see figure). The TaqMan® Fast Universal PCR Master Mix is formulated to deliver sensitive and reproducible results faster than is possible with standard reagents and instruments, and is robust across a wide range of target genes and concentrations.

resDNASEQ™ Quantitative Vero DNA Kit Applied Biosystems™

The resDNASEQ Quantitative Vero DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Vero (African Green Monkey) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of Vero cell DNA, typically in less than four hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high molecular weight or sheared DNA.

This kit does not include sample preparation reagents—for this, please see resDNASEQ Quantitative Vero DNA Kit with PrepSEQ Residual DNA Sample Preparation Kit.

• Highly sensitive quantitation using proven TaqMan real-time qPCR technology
• Offers consistent performance across the expected range of DNA fragment sizes
• Integrated system includes sample preparation kit, master mix, TaqMan primer/probe mix, and Vero genomic DNA standard
• Manual and automated sample preparation, optimized for quantitative recovery from complex sample matrices

The resDNASEQ Quantitative Vero DNA Kit is designed to provide highly sensitive detection of Vero DNA, allowing you to use small sample volumes to help generate accurate results. The broad linear range of TaqMan real-time qPCR technology allows you to test samples with variable levels of Vero DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts.

Easy to use, results in under 4 hours
The easy workflow of the resDNASEQ Quantitative Vero DNA Kit consists of sample preparation, assay setup, and instrument run and data analysis—all of which you can perform typically in under 4 hours. Start by extracting DNA from your in-process purification and drug substance samples. Then perform qPCR analysis to compare the amount of Vero DNA in your test samples to a standard curve generated with known amounts of purified Vero genomic DNA standard. The resDNASEQ system includes almost everything you need to carry out these steps, from sample preparation kit to master mix to TaqMan primer/probe mix to Vero genomic DNA standard, as well as step-by-step instructions.

Consistent performance even with fragmented DNA
For accurate quantitation of residual Vero DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample. With the resDNASEQ system you get consistent assay performance across a range of DNA fragment sizes.
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    Thermo Fisher Scientific - Site Down

    Sorry, our website is temporarily unavailable

    Thank you for visiting us—we appreciate your business

    Our website is temporarily unavailable while we make enhancements to improve your experience. Sorry for the inconvenience and please check back later or contact us via phone during normal business hours.

    North America

    United States: 1 760 603 7200
    (Toll Free): 1 800 955 6288

    Europe

    United Kingdom:
    +44 (0) 141 814 6100
    (Free phone): +44 (0) 800 269 210

    Service & Support

    Have a question or want to speak with a live representative?

    Phone   US Only: 1 800 955 6288

    North America

    Canada

    800 263 6236

    United States

    800 955 6288

    South America

    Argentina

    54 11 4556 0844

    Brasil

    0800 722 5433

    Chile

    56 2 228875500

    México

    52 (55) 3098 3800

    Europe

    Austria

    0800 20 10 87

    Belgium

    0800 14894

    Denmark

    80 30 17 40

    France

    0800 23 20 79

    Germany

    0800 083 09 02

    Italy

    800 872 987

    Norway

    00800 5345 5345

    Netherlands

    0800 099 3310

    Portugal

    800 206 639

    Spain

    900 181 461

    Sweden

    020 26 34 52

    Switzerland

    0800 84 88 00

    United Kingdom

    0800 269 210

    Asia Pacific

    Australia

    1300 735 292

    China (Beijing)

    800 820 8982

    China (Guangzhou)

    86 800 820 8982

    China (Shanghai)

    86 800 820 8982

    China (Hong Kong)

    852 2407 8450

    Japan

    81 3 6832 6980

    New Zealand

    0800 933 966

    Singapore

    65 6861 8638

    China (Taiwan)

    886 2 2358 2838