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Genomics and Proteomics


Antibiotic Spectrum


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Without Additives

Bacto™ Neopeptone Gibco™

Bacto Neopeptone is an animal origin (AO) enzymatic digest of bovine (milk) and porcine protein. This nutritional supplement provides vitamins, nucleotides, minerals, and peptides in a variety of sizes to any culture formulation. Customers have used Bacto Neopeptone as a component of culture media in the cultivation of human pathogens, notably Bordetella pertussis and group A streptococci, as well as Borrelia burgdorferi and Clostridium species, to produce vaccines for animal health applications.

Features:
• Enzymatic digest of bovine (milk) and porcine protein
• Animal origin nutritional supplement
• Contains a wide variety of peptide sizes in combination with vitamins, nucleotides, and minerals

Applications of Bacto Neopeptone include:
• Recommended for use in media for detection of fungi
• Reported to provide nutrients for support of spirochetes and protozoa
• Microbial fermentation
• Production of vaccines for animal health applications

Difco™ Beef Extract Gibco™

Difco Beef Extract is an animal origin peptone derived from an infusion of beef. This nutritional supplement provides a mixture of peptides, individual amino acids, nucleotides, organic acids, minerals, and vitamins. Provided as a paste, customers have used Difco Beef Extract in a variety of microbial fermentation cell lines, including Escherichia coli, Bacillus subtillis, Pasteurella multocida, and Streptococcus species to produce numerous animal health vaccines.

Features:
• An animal origin nutritional supplement
• A mixture of peptides, individual amino acids, nucleotides, organic acids, minerals, and vitamins

Applications of Difco Beef Extract include:
• Use in a variety of microbial fermentation cell lines
• Used to produce numerous animal health vaccines

Feed Kit C+ Gibco™

The Feed Kit C+ provides two chemically defined supplements, GlygcanTune™ C+ Total Feed and EfficientFeed™ C+ Supplement, along with fed-batch supplementation strategies designed to optimize cell growth and protein production while offering the ability to target and fine tune glycosylation profiles. GlycanTune C+ Total Feed is based on the chemically-defined EfficientFeed C+ Supplement and is specifically designed for fed-batch cultures of multiple cell lines. When used alone or with EfficientFeed+ supplement, this single-part total feeding supplement can shift glycan profiles from heavily G0F to mostly G1F and G2F glycans. GlycanTune C+ Total Feed provides an easy-to-reconstitute, pH-neutral solution that can be super-concentrated for delivery of key nutrients at smaller volumes.

Dialing in a glycan profile
The manual included in the kit (and downloadable below) provides complete protocols for testing GlycanTune C+ Total Feed with EfficientFeed C+ Supplement or another feed to obtain a set of conditions similar to those shown in the charts below. The first chart depicts protocol conditions for establishing a glycan shift pattern and the second shows typical results from a shifting experiment to help you hone in on the best feeding strategy to achieve a desired galactosylation profile.

Simplified handling to reduce risk in scale-up
The ability to scale up using AGT dry format provides easier handling and storage in manufacturing and other bioprocess settings. Fewer parts and reconstitution steps mean fewer opportunities for operator error. Further, the ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature in the dark may lessen the chance of contamination by reducing the number of connections to the bioreactor to one or two. Customization of other feeding formulations is possible should that option suit your needs better after trying this kit. Contact your local Field Application Scientist through your Account Manager to learn more about our customization capabilities.

Cell Culture Contamination Detection Kit Invitrogen™

The Cell Culture Contamination Detection Kit is a simple and effective procedure for screening tissue cultures for contamination by microorganisms. The kit not only detects contaminants, but it also identifies the contaminant type. Three fluorescent dyes distinctly stain yeast (and other fungi), gram-positive and gram negative bacteria by slide preparation.

Resurge™ CD4 Supplement, without glucose and L-glutamine Gibco™

Resurge CD4 Supplement, without glucose and L-glutamine, is a chemically defined supplement formulation to complement a variety of base media. This supplement was developed using several host cell lines and in various culture systems, including shaker flasks and bench-scale bioreactors. Resurge CD4 Supplement, without glucose and L-glutamine, was developed for use in CHO mammalian culture systems and can be used either as a media supplement or a culture system feed.

Antibiotic-Antimycotic (100X) Gibco™

Gibco® Antibiotic-Antimycotic is used to prevent bacterial and fungal contamination. This solution contains 10,000 units/mL of penicillin, 10,000 µg/mL of streptomycin, and 25 µg/mL of Gibco Amphotericin B. The antibiotics penicillin and streptomycin prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Amphotericin B prevents fungal contamination of cell cultures due to its inhibition of multi-cellular fungus and yeast.

Penicillin was originally purified from the fungus Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus. It acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Amphotericin B is an antifungal agent that prevents the growth of fungi and yeast by causing an increase in fungal plasma membrane permeability.

pHrodo™ Red S. aureus Bioparticles™ Conjugate for Phagocytosis Invitrogen™

New pH-sensitive pHrodo® Red dye conjugates give faster and more accurate results than any other phagocytosis assay pHrodo® Red dye conjugates are non-fluorescent outside the cell, but fluoresce brightly red in phagosomes.

Get faster staining and more accurate results without the need for wash steps or quencher dye
• Specific detection of phagocytosis and endocytosis
• Reduced signal variability and improved timing in sensitive experiments
• Multiplex with green dyes such as GFP, Fluo-4, or calcein

The fluorescence of the novel pHrodo® Red dye dramatically increases as pH decreases from neutral to the acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes. Use the ready-made pHrodo® Red S. aureus BioParticles® conjugates in imaging or flow applications, or pHrodo® Red SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.

Cotton Peptone 200, ultra-filtered (UF) Gibco™

Cotton 200, UF, is rich in nucleoside content and digested to enhance peptide content. It is processed to provide higher concentrations of vitamins, which can help maintain performance in mammalian and microbial processes.

Cotton 200, UF, is also part of the Peptone Preview Pak which contains:
• Cotton 100, UF—rich in nucleoside content and digested to enhance peptide content. It is processed to provide higher concentrations of vitamins, which can help maintain performance in mammalian and microbial processes.
• Wheat 100, UF—ultra-filtered supplement and good source of glutamine, which makes it ideal for mammalian cell culture applications. It is digested for enhanced peptide content resulting in a low concentration of free amino acids and carbohydrates.
• Soy 100—highly digested soy protein peptone that is high in nucleosides and free amino acid content. It has high concentrations of vitamins and carbohydrates and is recommend for a wide variety of processes.
• Pea 100, UF—ultra-filtered peptone digested to enhance nucleoside and free amino acid content. The high total amino acid content and low carbohydrate content are similar to many high-performance animal origin peptones.
• Yeast 100—designed to enhance the content of vitamins and other key nutritional components found in yeast. It is intended for nutritional supplementation for mammalian, microbial, and insect cell culture.

Peptone Preview Pak supplements are non-GMP prototype products that are not for manufacture use. Larger sizes are available through the Gibco Custom Products Services.

Trypsin/EDTA Solution (TE) Gibco™

Gibco® Trypsin solutions are made from trypsin powder - a mixture of proteases derived from bovine pancreas. Due to its digestive strength, it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation. This Gibco® Trypsin/EDTA solution is formulation as 0.025% trypsin and 0.01% EDTA in Phosphate Buffered Saline (PBS). We offer a variety of Gibco® Trypsin and animal origin free TrypLE™ cell dissociation products to meet your needs.

This trypsin is manufactured as follows:

With
EDTA
Phenol Red
The complete formulation is available.

Product Use
For Research Use Only. Not for use in diagnostic procedures.

cGMP Manufacturing and Quality System
Gibco® Trypsin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Escherichia coli (K-12 strain) BioParticles™, Alexa Fluor™ 594 conjugate Invitrogen™

The Molecular Probes® BioParticles® product line consists of a series of fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities. These fluorescent BioParticles® products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry.

We offer E. coli (K-12 strain), S. aureus (Wood strain, without protein A) and zymosan (S. cerevisiae) BioParticles® conjugates covalently labeled with a variety of different fluorophores (special care has been taken to remove free dye after conjugation). Unlike the fluorescence of fluorescein-labeled BioParticles® conjugates, which is partially quenched in acidic environments, the fluorescence of the Alexa Fluor®, BODIPY® FL, tetramethylrhodamine and Texas Red® dye conjugates is uniformly intense over the pH range from 3 to 10.

BioParticles Specifications:
• Label (Ex/Em): Alexa Fluor® 594 (~590/617 nm)
• Particle: E. coli (K-12 strain)
Opsonizing reagent available


Using BioParticles Products
BioParticles® conjugates are provided as lyophilized powders. There are approximately 3 x 108 E. coli or S. aureus particles per mg solid and approximately 2 x 107 zymosan particles per mg solid. BioParticles® conjugates can be reconstituted in the buffer of your choice for use in phagocytosis assays. The fluorescence of BioParticles® conjugates that are bound to the surface of the cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers. In addition to cellular applications, fluorescent BioParticles® conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants. These small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways.

Find More BioParticles® Products
We offer a large range of dye-labeled and unlabeled E. coli (K-12 strain), S. aureus (Wood strain, without protein A), and zymosan (S. cerevisiae) BioParticles® products. Find out about these products and their applications by reviewing Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook.

For pH-sensitive endocytosis assays, see our pHrodo™ BioParticles® conjugates.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 647 Streptavidin (Alexa Fluor™ 647 EGF complex) Invitrogen™

We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green® 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor® dyes and Texas Red® dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor.

Epidermal Growth Factor Conjugate Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Biotin-XX complexed with Alexa Fluor® 647 streptavidin (~650/665 nm)
• Number of fluorophore molecules on each EGF molecule: ~2–3
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter


Find More Receptor Binding and Phagocytosis Probes
We offer a number of fluorescently labeled probes for studying receptor-mediated endocytosis, membrane markers for endocytosis and exocytosis, and methods for detecting internalized fluorescent ligands. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.

KaryoMAX™ Colcemid™ Solution in PBS Gibco™

Gibco® KaryoMAX® Colcemid™ Solution in PBS is a 10 µg/ml N-desacetyl-N-methylocolchicine (Colcemid™) solution made in Phosphate Buffered Saline (PBS). It is used for arresting cells in metaphase so the chromosomes can be separated for cytogenetic studies and in vitro diagnostic procedures. Colcemid™ prevents spindle formation during mitosis, causing metaphase arrest. The mechanism of action is similar to that of colchicine, but with lower mammalian toxicity. The complete formulation is available.

Product Use
For in vitro diagnostic use.

cGMP Manufacturing and Quality System
Gibco® KaryoMAX® Colcemid™ Solution in PBS is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Bacto™ Proteose Peptone Gibco™

Bacto Proteose Peptones are enzymatic digests of protein. Studies of peptic digests of animal tissue prepared under varying digestion parameters demonstrated that no single peptone is the most suitable nitrogen source for every microbiological application. These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone.

• Bacto Proteose Peptone is used in preparing microbiological culture media and in producing bacterial toxins
• Bacto Proteose Peptone No. 2 is used in preparing microbiological culture media
• Bacto Proteose Peptone No. 3 is used in preparing microbiological and mammalian cell culture media
• Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone. It offers the same beneficial nutrients as Bacto Proteose Peptone for growth promotion and toxin production in a wide range of fastidious microorganisms.

We also offer alternatives to Bacto Proteose Peptones for scale-up to production applications, including BiTek Proteose Peptone.

KnockOut™ Serum Replacement Gibco™

Knockout™ Serum Replacement (Knockout™ SR) is for serum-free, feeder-based culture of embryonic stem cells (ESCs) and induced Pluripotent stem cells (iPSCs) from multiple species. This defined, serum-free formulation directly replaces FBS in existing protocols.

Many of the drawbacks of using FBS in stem cell culture, (differentiation of cells and heat-inactivation) can be alleviated with the use of KnockOut™ Serum Replacement. ES and iPS cells grown in KnockOut™ SR-supplemented media are substantially less differentiated than those grown in FBS-supplemented media, and germline transmission should not be compromised. With over 2000 peer-reviewed publications covering applications such as stem cell culture, differentiation, iPSC generation, ESC derivation, and transgenic modeling, KnockOut™ Serum Replacement is widely cited for stem cell culture.

Trypsin Neutralizer Solution Gibco™

A sterile, phosphate-buffered saline solution (1X) containing calf serum as a trypsin inhibitor.
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