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Genomics and Proteomics


Protein Family


GlutaMAX™ Supplement Thermo Scientific™

Gibco® GlutaMAX™ Supplement is an alternative to L-glutamine, with increased stability that improves cell health. GlutaMAX™ Supplement is suitable for both adherent and suspension culture of mammalian cells, with no adaptation required. GlutaMAX™ Supplement is offered as a 200 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl. GlutaMAX™ Supplement is also included in a variety of media formulations. Compared to L-glutamine, GlutaMAX™ Supplement:

Features:

Minimizes toxic ammonia build-up
Improves cell viability and growth
Remains stable across a wide range of temperatures

Minimizes toxic ammonia build-up
Unlike L-glutamine, GlutaMAX™ Supplement does not spontaneously break down to form ammonia. Instead, cells cleave the dipeptide bond to release L-glutamine as needed. This system prevents waste build-up and maintains a fresh supply of L-glutamine during long-term culture.

Improves cell viability and growth
Cultures with lower levels of ammonia show improved cell viability. In addition, culture conditions are more controlled due to the consistent concentration of GlutaMAX™ Supplement that is not possible with L-glutamine.

Remains stable across a wide range of temperatures
GlutaMAX™ Supplement is room-temperature stable and ready to use. GlutaMAX™ Supplement retains full functionality for 24 months at room temperature, 2 to 8°C, or -5 to -30°C for convenient storage and handling. GlutaMAX™ Supplement is even stable at 37°C for a week or more.

cGMP manufacturing and quality system
Gibco® GlutaMAX™ supplement is manufactured at a cGMP compliant facility, located in Paisley, Scotland. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® GlutaMAX™ supplement made in our Grand Island, New York facility (35050-061). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Gentamicin (10 mg/mL) Gibco™

Gentamicin sulfate is a water-soluble antibiotic drug originally purified from the fungus Micromonospora purpurea. Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition protein synthesis and death in susceptible bacteria. Gibco® Gentamicin is effective against a wide variety of gram-positive and gram-negative bacteria, and is used for the prevention of bacterial contamination on cell cultures. The recommended working concentration ranges from 0.5 to 50 µg/ml. We offer a variety of antibiotics and antimycotics for cell culture applications.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Dual-Site cGMP Manufacturing
Gibco® Gentamicin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® Gentamicin product made in our Scotland facility (15710-049). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Bacto™ Neopeptone Gibco™

Bacto Neopeptone is an animal origin (AO) enzymatic digest of bovine (milk) and porcine protein. This nutritional supplement provides vitamins, nucleotides, minerals, and peptides in a variety of sizes to any culture formulation. Customers have used Bacto Neopeptone as a component of culture media in the cultivation of human pathogens, notably Bordetella pertussis and group A streptococci, as well as Borrelia burgdorferi and Clostridium species, to produce vaccines for animal health applications.

Features:
• Enzymatic digest of bovine (milk) and porcine protein
• Animal origin nutritional supplement
• Contains a wide variety of peptide sizes in combination with vitamins, nucleotides, and minerals

Applications of Bacto Neopeptone include:
• Recommended for use in media for detection of fungi
• Reported to provide nutrients for support of spirochetes and protozoa
• Microbial fermentation
• Production of vaccines for animal health applications

GlutaMAX™ Supplement Gibco™

Gibco® GlutaMAX™ Supplement is an alternative to L-glutamine, with increased stability that improves cell health. GlutaMAX™ Supplement is suitable for both adherent and suspension culture of mammalian cells, with no adaptation required. GlutaMAX™ Supplement is offered as a 200 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl. GlutaMAX™ Supplement is also included in a variety of media formulations. Compared to L-glutamine, GlutaMAX™ Supplement:

Minimizes toxic ammonia build-up
Improves cell viability and growth
Remains stable across a wide range of temperatures

Minimizes toxic ammonia build-up
Unlike L-glutamine, GlutaMAX™ Supplement does not spontaneously break down to form ammonia. Instead, cells cleave the dipeptide bond to release L-glutamine as needed. This system prevents waste build-up and maintains a fresh supply of L-glutamine during long-term culture.

Improves cell viability and growth
Cultures with lower levels of ammonia show improved cell viability. In addition, culture conditions are more controlled due to the consistent concentration of GlutaMAX™ Supplement that is not possible with L-glutamine.

Remains stable across a wide range of temperatures
GlutaMAX™ Supplement is room-temperature stable and ready to use. GlutaMAX™ Supplement retains full functionality for 24 months at room temperature, 2 to 8°C, or -5 to -30°C for convenient storage and handling. GlutaMAX™ Supplement is even stable at 37°C for a week or more.

cGMP manufacturing and quality system
Gibco® GlutaMAX™ Supplement is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® PBS product made in our Scotland facility (35050-087). This facility is also registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gurr Buffer Tablets Gibco™

Gibco® Gurr Buffer Tablets offer a convenient way to prepare a pH 6.8 phosphate buffer for dilution of Giemsa stain used in G-banding of chromosomes for cytogenetic analysis. Simply add one buffer tablet to one liter of distilled water and stir until dissolved.

Ampicillin, sodium salt, irradiated Gibco™

Ampicillin is an antibiotic in the broad-spectrum penicillin group. Ampicillin differs from penicillin only by the presence of an amino group, which facilitates penetration through the outer membrane of some gram-negative bacteria. Ampicillin acts by interfering directly with the turnover of the bacterial cell wall and indirectly by triggering the release of enzymes that further alter the cell wall.

Gibco Ampicillin is used as a selective antibiotic generally at a concentration of 20–125 µg/mL. Ampicillin selection is based on hydrolysis and inactivation of its beta-lactam ring by beta-lactamase expressed by the bla gene. Sometimes, beta-lactamase produced by the bacteria is secreted into the medium resulting in inactivation of ampicillin in the culture medium. Ampicillin degradation on agar plates can lead to the formation of satellite colonies. Growth of these colonies can be halted by adding fresh ampicillin. To avoid beta-lactamase buildup, special care must be taken while handling cultures and determining the correct dose of antibiotic.

Ampicillin antibiotic in powder form is an economical choice that can be used in a wide range of gram-positive and gram-negative infections. This product is provided as a powder and should be made into a stock solution at a concentration of 10 mg/mL in water.

cGMP manufacturing
Gibco Ampicillin is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Bacto™ Yeast Extract, technical Gibco™

Bacto Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells. They are derived from primary grown baker’s yeast. These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications. Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation.

Bacto Yeast Extract is one of the most complete and versatile fermentation supplements available. It is an important ingredient for the microbiological assay of vitamins. Yeast extract is also of value in the assay of antibiotics. B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract. Bacto Yeast Extract, Technical, was developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics.

Related yeast extract products:
• Gibco Yeast Extract—developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Difco Yeast Extract UF—ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g.
• Difco Yeast Extract Low-Dusting (LD)—designed to limit the amount of dust generated compared to other spray-dried yeast extracts

Sodium Bicarbonate 7.5% solution Gibco™

Sodium Bicarbonate is buffer commonly used for maintaining the pH of cell culture medium in the presence of 4–10% carbon dioxide. In addition to buffering, sodium bicarbonate provides some nutritional benefit, while rarely displaying any cell toxicity. Formulated as 75 g per liter of water, Gibco® Sodium Bicarbonate, 7.5% Solution is perfect for supplementing dry powder medium during reconstitution. The final Sodium Bicarbonate concentration used in the medium depends on the media formulation and the carbon dioxide concentration used in the incubator.

Product Use
For Research Use Only. Not for use in diagnostic procedures.

Dual-site cGMP Manufacturing and Quality System
Gibco® Sodium Bicarbonate Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® Sodium Bicarbonate Solution product made in our Scotland facility (25080-060). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix Gibco™

Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix is a soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors and is free of viruses, including lactose dehydrogenase elevating virus (LDEV), sometimes found in this type of preparation. The major components of Geltrex™ matrix include laminin, collagen IV, entactin, and heparin sulfate proteoglycans. It is ideal for the promotion and maintenance of differentiated phenotypes in a variety of cell cultures including primary epithelial cells, endothelial cells, smooth muscle cells, and human induced pluripotent stem cells (iPSC). Geltrex™ matrix offers, on average, a 2-fold reduction in several growth factors. As a result, analysis of specific growth factor effects in applications requiring basement membrane extract can be carried out in a controlled and reproducible format.

Key Features of Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix:

• Free of lactose dehydrogenase elevating virus (LDEV) making it ideal for all types of cell culture and mouse in vivo applications
• Consistent protein concentration lot-to-lot helps reduce the need to screen lots
• Protocols for various applications, including tube formation assays, are available

Gain Reliability and Consistency in Your hESC Studies
Recent advances in processing EHS tumor cells have resulted in virus free basement membrane extracts. Every lot is tested by RT-PCR methods so you can now have confidence that every lot of Geltrex™ is virus free. Additionally, our consistent manufacturing process helps ensure a protein concentration with very little variation from lot-to-lot, averaging 15 mg/ml so you can have confidence in every purchase.

Investigate Angiogenesis with Validated Gibco® Protocols
There are many applications for Geltrex™ LDEV-Free reduced growth factor basement membrane matrix, each requiring different thicknesses and concentrations. In general, a protein concentration of >5 mg/ml is used for differentiation studies of primary cells. Geltrex™ LDEV-Free reduced growth factor basement membrane matrix has been used to develop tube formation assay protocols using Gibco® primary cells and specialty media. See this and other angiogenesis assay protocols

Insulin, human recombinant, zinc solution Gibco™

4 mg/ml

Potency: (dry basis) >= 27 USP units/mg.
Suggested range for supplementation: 2 to 20 µg/ml.

Escherichia coli (K-12 strain) BioParticles™, fluorescein conjugate Invitrogen™

The Molecular Probes® BioParticles® product line consists of a series of fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities. These fluorescent BioParticles® products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry.

We offer E. coli (K-12 strain), S. aureus (Wood strain, without protein A) and zymosan (S. cerevisiae) BioParticles® conjugates covalently labeled with a variety of different fluorophores (special care has been taken to remove free dye after conjugation). Unlike the fluorescence of fluorescein-labeled BioParticles® conjugates, which is partially quenched in acidic environments, the fluorescence of the Alexa Fluor®, BODIPY® FL, tetramethylrhodamine and Texas Red® dye conjugates is uniformly intense over the pH range from 3 to 10.

BioParticles Specifications:
• Label (Ex/Em): Fluorescein (~494/518 nm)
• Particle: E. coli (K-12 strain)
Opsonizing reagent available


Using BioParticles Products
BioParticles® conjugates are provided as lyophilized powders. There are approximately 3 x 108 E. coli or S. aureus particles per mg solid and approximately 2 x 107 zymosan particles per mg solid. BioParticles® conjugates can be reconstituted in the buffer of your choice for use in phagocytosis assays. The fluorescence of BioParticles® conjugates that are bound to the surface of the cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers. In addition to cellular applications, fluorescent BioParticles® conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants. These small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways.

Find More BioParticles® Products
We offer a large range of dye-labeled and unlabeled E. coli (K-12 strain), S. aureus (Wood strain, without protein A), and zymosan (S. cerevisiae) BioParticles® products. Find out about these products and their applications by reviewing Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook.

For pH-sensitive endocytosis assays, see our pHrodo™ BioParticles® conjugates.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Staphylococcus aureus (Wood strain without protein A) BioParticles™, unlabeled Invitrogen™

The Molecular Probes® unlabeled BioParticles® product line comprises heat- or chemically killed S. aureus (Wood strain, without protein A) and zymosan (S. cerevisiae) cells. These nonfluorescent BioParticles® products are useful in phagocytosis assays, both as controls and for custom-labeling with reactive dyes.

BioParticles Specifications:
• Label (Ex/Em): Unlabeled
• Particle: S. aureus (Wood strain, without protein A)
Opsonizing reagent available


Using BioParticles® Products
BioParticles® conjugates are provided as lyophilized powders. There are approximately 3 x 108 S. aureus particles per mg solid and approximately 2 x 107 zymosan particles per mg solid. BioParticles® conjugates can be reconstituted in the buffer of your choice for use in phagocytosis assays.

Find More BioParticles® Products
We offer a large range of dye-labeled and unlabeled E. coli (K-12 strain), S. aureus (Wood strain, without protein A), and zymosan (S. cerevisiae) BioParticles® products. Find out about these products and their applications by reviewing Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook.

For pH-sensitive endocytosis assays, see our fluorescent pHrodo™ BioParticles® conjugates.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Bacto™ Proteose Peptone Gibco™

Bacto Proteose Peptones are enzymatic digests of protein. Studies of peptic digests of animal tissue prepared under varying digestion parameters demonstrated that no single peptone is the most suitable nitrogen source for every microbiological application. These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone.

• Bacto Proteose Peptone is used in preparing microbiological culture media and in producing bacterial toxins
• Bacto Proteose Peptone No. 2 is used in preparing microbiological culture media
• Bacto Proteose Peptone No. 3 is used in preparing microbiological and mammalian cell culture media
• Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone. It offers the same beneficial nutrients as Bacto Proteose Peptone for growth promotion and toxin production in a wide range of fastidious microorganisms.

We also offer alternatives to Bacto Proteose Peptones for scale-up to production applications, including BiTek Proteose Peptone.

Histone H1 From Calf Thymus, Alexa Fluor™ 488 Conjugate Invitrogen™

The Alexa Fluor® 488 histone H1 conjugate is labeled with the bright and photostable Alexa Fluor® 488 dye and exhibits fluorescence with excitation/emission maxima ~495/520 nm and can be visualized by fluorescence microscopy or flow cytometry.

CTS™ LV-MAX™ Transfection Kit Gibco™

The Gibco CTS LV-MAX Transfection Kit is an essential component of the CTS LV-MAX lentiviral production system. Based on extensive Design of Experiment (DOE) methodologies, the cationic lipid-based transfection reagent, lentiviral production supplement, and LV enhancer are specifically screened for maximal delivery of multiple DNA plasmids to achieve the highest possible lentiviral titer when used in conjunction with CTS Viral Production Cells cultured in CTS LV-MAX Production Medium.

All CTS LV-MAX Transfection Kit components are produced under cGMP manufacturing conditions with extensive quality-control testing to ensure proper preclinical, clinical, and commercial therapeutic viral vector applications.

CTS LV-MAX Transfection Kit features include:
• Uniquely designed to enhance transient transfection of multiple DNA plasmids into high density suspension HEK293-derived cell culture
• Enables high viral titers of up to 1 x 108 TU/mL (unconcentrated LVV-GFP) to allow for a cost-effective alternative to PEI-based transfection reagents
• Scalable protocol for 96-deep well blocks, flasks, and bioreactors
• Regulatory-compliant reagents produced in conformity with cGMP for medical devices, 21 CFR, Part 820, and certified to the ISO 13485. Drug Master File and Regulatory Support File available upon request.

The CTS LV-MAX Transfection Kit requires CTS Opti-MEM I (not included in this kit) for complexation. The chemically-defined, serum-free, animal origin-free features of the CTS LV-MAX Transfection Kit result in high reproducibility and lot-to-lot reliability. The formulation of this product is identical to the research-grade LV-MAX Transfection Kit, but the CTS LV-MAX Transfection Kit is validated for clinical and commercial therapeutic applications.

Custom configuration and packaging available upon request.

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