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Genomics and Proteomics


Protein Family


Bacto™ Peptone Gibco™

Bacto Peptone is an animal origin (AO) enzymatic digest of bovine and porcine animal proteins. The nutritive value of Bacto Peptone is largely dependent on a combination of a negligible quantity of proteases, high concentration of complex constituents, and a unique amino acid content that makes this peptone a strong nitrogen source. Customers have successfully used this supplement with a variety of cell types including Saccharomyces cerevisiae, Escherichia coli, Pasteurella multocida, Vibrio cholerae, and porcine kidney cells to produce biotherapeutics and vaccines for animal health applications.

Features:
• A strong nitrogen source
• Unique amino acid content
• High concentration of complex constituents

Applications of Bacto Peptone include:
• Vaccines for animal health applications
• Production of biotherapeutics
• Mammalian cell culture
• Microbial fermentation

Gurr Buffer Tablets Gibco™

Gibco® Gurr Buffer Tablets offer a convenient way to prepare a pH 6.8 phosphate buffer for dilution of Giemsa stain used in G-banding of chromosomes for cytogenetic analysis. Simply add one buffer tablet to one liter of distilled water and stir until dissolved.

Acidicase™ Peptone Gibco™

Acidicase Peptone is a hydrochloric acid hydrolysate of casein. The manufacturing process produces a casein hydrolysate, which has a high salt content of approximately 37% and nitrogen content of approximately 8%. The hydrolysis of the casein, a milk protein rich in amino acid nitrogen, is carried out until all the nitrogen is converted to amino acids or other compounds of relative simplicity. Casein contains little cystine and is deficient in tryptophan, which is destroyed by the acid treatment.

Features:
• Hydrochloric acid hydrolysate of casein
• Animal origin (AO) nutritional supplement
• Milk protein rich in amino acid nitrogen

Applications of Acidicase Peptone include:
• Intended for use as a nutritional supplement in vitamin assays, susceptibility testing, and other laboratory media and microbial fermentation in which the high salt content will not interfere

Bacto™ Yeast Extract Gibco™

Bacto Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells. They are derived from primary grown baker’s yeast. These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications. Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation.

Bacto Yeast Extract is one of the most complete and versatile fermentation supplements available. It is an important ingredient for the microbiological assay of vitamins. Yeast extract is also of value in the assay of antibiotics. B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract. Bacto Yeast Extract, Technical, was developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics.

Related yeast extract products:
• Gibco Yeast Extract—developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Difco Yeast Extract UF—ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g.
• Difco Yeast Extract Low-Dusting (LD)—designed to limit the amount of dust generated compared to other spray-dried yeast extracts

Bacto™ Proteose Peptone No. 3 Gibco™

Bacto Proteose Peptone No. 3 is a member of the Bacto Proteose Peptones, which are enzymatic digests of protein. Studies of peptic digests of animal tissue prepared under varying digestion parameters demonstrated that no single peptone is the most suitable nitrogen source for every microbiological application. These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone.

• Bacto Proteose Peptone is used in preparing microbiological culture media and in producing bacterial toxins
• Bacto Proteose Peptone No. 2 is used in preparing microbiological culture media
• Bacto Proteose Peptone No. 3 is used in preparing microbiological and mammalian cell culture media
• Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone. It offers the same beneficial nutrients as Bacto Proteose Peptone for growth promotion and toxin production in a wide range of fastidious microorganisms.

We also offer alternatives to Bacto Proteose Peptones for scale-up to production applications, including BiTek Proteose Peptone.

EfficientFeed™ C+ 2X Supplement Gibco™

Gibco EfficientFeed C+ 2X Supplement is a single-part liquid supplement for fed batch addition to the culture of multiple cell lines to assist with process development and maximization of bioreactor utilization. This product is designed to work with CHO cell lines expressing endogenous glutamine synthetase (GS) as a single feed without the need for additional GS supplements. Compared to the original CD EfficientFeed C AGT nutrient supplement, EfficientFeed C+ 2X Supplement has components that are super-concentrated from 81.2 g/L (1X AGT) to 162.4 g/L (2X), helping reduce product dilution or increase titers with additional feeding. The product is an animal origin-free, chemically defined formulation that contains no proteins, hydrolysates, or components of incompletely defined composition.

Which EfficientFeed+ supplement to choose?

Consider EfficientFeed A+ Supplement when:
• You have used CHO CD EfficientFeed A supplement in the past and wish to explore simpler reconstitution and super-concentration benefits during delivery of key components
• You are currently using CD OptiCHO as your base cell growth medium

Consider EfficientFeed B+ Supplement when:
• You have used CHO CD EfficientFeed B supplement in the past and wish to explore simpler reconstitution and super-concentration benefits during delivery of key components
• You are currently using CD CHO as your base cell growth medium

Consider EfficientFeed C+ Supplement when:
• You have used CD EfficientFeed C supplement in the past and wish to explore super-concentration benefits during delivery of key components
• You want higher productivity in GS CHO, CHO-K1, and CHO-S derived cell lines
• You are currently using CD FortiCHO as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at Day 0

Increasing titers
As a complete feed, EfficientFeed+ supplements have been shown to provide multiple-fold increases in titer versus batch culture and are an excellent starting point for process development as ready-to-use single-part liquid feeds.

Maximizing bioreactor utilization
Have you ever wished for more working volume at the start of a bioreactor run to produce more protein, but need too much feeding volume space? Have you ever thought that room for more feeding might achieve a new level of titer from a process and maybe even reduce the number of bioreactor runs needed per year? Using super-concentrated ready-to-feed liquid feeding supplements helps reduce product dilution, increase space for additional feeding, and/or increase the working volume at the start of the run. In fact, you may gain about 20% more bioreactor utilization with this super-concentrated EfficientFeed+ product.

Simplified handling to reduce risk in scale-up
The ability to scale up in liquid or AGT dry format provides ease of handling and storage in manufacturing and other bioprocess settings. Fewer parts and steps in reconstitution mean fewer opportunities for operator error. Further, the ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature, in the dark, helps reduce chances of contamination during closed system connections by reducing the number of connections to the bioreactor to one.

Resurge™ CD2 Supplement, without glucose and L-glutamine Gibco™

Resurge CD2 Supplement, without glucose and L-glutamine, is a chemically defined supplement formulation to complement a variety of base media. This supplement was developed using several host cell lines and in various culture systems, including shaker flasks and bench-scale bioreactors. Resurge CD2 Supplement, without glucose and L-glutamine, was developed for use in CHO mammalian culture systems and can be used either as a media supplement or a culture system feed.

Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 647 Streptavidin (Alexa Fluor™ 647 EGF complex) Invitrogen™

We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green® 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor® dyes and Texas Red® dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor.

Epidermal Growth Factor Conjugate Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Biotin-XX complexed with Alexa Fluor® 647 streptavidin (~650/665 nm)
• Number of fluorophore molecules on each EGF molecule: ~2–3
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter


Find More Receptor Binding and Phagocytosis Probes
We offer a number of fluorescently labeled probes for studying receptor-mediated endocytosis, membrane markers for endocytosis and exocytosis, and methods for detecting internalized fluorescent ligands. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.

Bacto™ Tryptone Gibco™

Bacto Tryptone is an animal origin (AO) pancreatic digest of casein. It is included in standard methods manual applications and is listed in the Reagent section of the United States Pharmacopeia as meeting the specifications for a pancreatic digest of casein, a component in many of the media listed. The nutritional profile of this supplement is unique in that it provides a range of nutrients. Customers have used this supplement in fermentation applications and shown that it successfully supports the growth of many bacterial species during the production of animal health vaccines, including Escherichia coli, and Fusobacterium and Agrobacterium species when used together with Bacto Yeast Extract.

Features:
• Pancreatic digest of casein
• Developed while investigating a peptone particularly suitable for the elaboration of indole by bacteria
• Notable for the absence of detectable levels of carbohydrates
• Animal origin nutritional supplement

Applications of Bacto Tryptone include:
• Works well in fermentation applications
• Use with common organisms such as Escherichia coli, as well as uncommon organisms such as the diatom Nitzschia laevis

Propidium Iodide Ready Flow™ Reagent Invitrogen™

Propidium Iodide Ready Flow™ Reagent is a bright, easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays. This dye has excitation/emission maxima of 535/617 nm.

• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh. or pipette
• Rapid staining of dead cells without wash steps

Propidium Iodide Ready Flow Reagent is a commonly used dead-cell stain that emits red fluorescence when bound to DNA. Add 2 drops from the dropper bottle directly into 1 x 106 cells, incubate, and analyze.

Propidium iodide is membrane impermeant and therefore excluded from viable cells. It binds to DNA by intercalating between the bases, thereby binding both DNA and RNA. Propidium iodide is commonly excited with the 488 nm laser, but others such as the 532 and 561 nm lasers are also suited for PI excitation.

Bacto™ Casamino Acids Gibco™

Bacto Casamino Acids is an animal origin (AO) acid hydrolysis of casein. This supplement contains low salt and iron content making it an excellent supplement for media formulations where nitrogen requirements are minimal. This supplement is successfully used in fermentation applications for pneumococcal, diphtheria, pertussis, tetanus, and zoster vaccines. It maintains many of the nutritional benefits of hydrolyzed casein, but is further supplemented with growth factors, cystine, maltose, and an optimum amount of iron and inorganic salts.

Customers have used Bacto Casamino Acids to support the growth of Bordetella, Corynebacterium, Pseudomonas, Haemophilus, and Escherichia species. Additionally, together with Bacto Yeast Extract, technical, customers have used this supplement successfully in the production of animal and human health vaccines.

Phytone™ Peptone Gibco™

Phytone Peptone is an animal origin-free (AOF) enzymatic digest of soy. Due to the naturally high concentrations of vitamins and carbohydrates in soy, Phytone Peptone is a nutritious and excellent carbohydrate source. Customers have successfully used this supplement in CHO-based production of monoclonal antibodies for oncological and rheumatological therapeutics, as well as with many different microbial cell types to produce animal health vaccines for swine, such as Clostridial vaccines.

Features:
• Animal origin-free (AOF) supplement
• Enzymatic digests of soybean meal/flour
• Retains the high vitamin and high carbohydrate content of the soy plant tissue

Applications of Phytone Peptone include:
• It is an excellent plant peptone for the cultivation of fungi and fastidious types of bacteria, such as members of the Clostridium and Neisseria genera
• It has been used in mammalian cell culture applications due to its high carbohydrate content

Polypeptone™ Peptone Gibco™

Polypeptone Peptone is a mixture of peptones made up of equal parts of pancreatic digest of casein and peptic digest of animal tissue. Polypeptone Peptone includes the high content of amino acids and small polypeptides characteristic of pancreatic digest of casein and the larger polypeptides characteristic of peptic digest of animal tissue.

Features:
• Animal origin mixture of peptones
• Includes the high content of amino acids

Applications of Polypeptone Peptone include:
• Found to meet nutritional requirements of various bacteria, fungi, and mammalian cells for which a single source of casein or meat peptones has been unsatisfactory

TrypLE™ Select Enzyme (10X), no phenol red Gibco™

TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A549, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE™ formulations for a range of cell culture applications.

Compared to trypsin and other dissociation reagents, Gibco® TrypLE™ Select is:

Gentle on cells—maintains cell health for reproducible results
Room-temperature stable—convenient to use
Easy to use—substitutes directly into existing protocols, with no inactivation required
Animal origin free (AOF)—pure, consistent, and effective

This TrypLE™ Select is modified as follows:

WithWithout

• EDTA

• Phenol Red

The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.

Gentle on cells
TrypLE™ Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE™ Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room-temperature stable
TrypLE™ Select is room-temperature stable and ready to use. TrypLE™ Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use
TrypLE™ Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Select, avoiding the need for trypsin inhibitors, such as FBS.

Animal Origin Free (AOF)
Unlike porcine trypsin and other enzymes, TrypLE™ Select is free of animal-derived components, and is formulated on dedicated animal origin-free equipment.

Product Intended Use
For Research Use or Further Manufacturing. Not for use in diagnostic procedures. Customers using Gibco® TrypLE™ Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco® TrypLE™ Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Concentrated forms of TrypLE™ can be aseptically diluted to 1x using a balanced salt solution containing 1 mM EDTA.

CHO Cell Culture Medium Gibco™

CHO Cell Culture Medium is a complete animal origin-free (AOF) and serum-free, ready-to-use formulation containing a plant hydrolysate for maximum productivity. This medium is formulated to support cell growth and production of antibodies and recombinant proteins in suspension CHO cell cultures. CHO Cell Culture Medium can be used in various culture vessels from T-flasks, shaker flasks, and roller bottles to spinners and bioreactors. The medium can be used in research or manufacturing applications. It has been developed after careful screening of a wide variety of hydrolysates for protein yield increases in CHO cells.

CHO Cell Culture Medium is appropriate for suspension CHO cells. It is also appropriate for a selection system such as glutamine synthetase (GS), which does not require the selective pressure of hypoxanthine/thymidine (HT)-deficient medium.

Features:
• Complete animal origin-free (AOF) and serum-free, ready-to-use formulation
• Supports cell growth and production of antibodies and recombinant proteins in suspension CHO cell cultures

Applications of CHO Cell Culture Medium:
• Appropriate for suspension CHO cells
• Appropriate for a selection system such as glutamine synthetase (GS), which does not require the selective pressure of hypoxanthine/thymidine (HT)-deficient medium

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