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Cotton 100, ultra-filtered (UF) Gibco™
Cotton 100, UF, is rich in nucleoside content and digested to enhance peptide content. It is processed to provide higher concentrations of vitamins, which can help maintain performance in mammalian and microbial processes.
Cotton 100, UF, is also part of the Peptone Preview Pak which contains:
• Cotton 200, UF—ultra-filtered and rich in nucleoside content, processed to enhance carbohydrate concentrations, which is important for some cell culture and microbial processes
• Wheat 100, UF—an ultra-filtered supplement and good source of glutamine, which makes it ideal for mammalian cell culture applications. It is digested for enhanced peptide content resulting in a low concentration of free amino acids and carbohydrates.
• Soy 100—a highly digested soy protein peptone that is high in nucleosides and free amino acid content. It has high concentrations of vitamins and carbohydrates and is recommend for a wide variety of processes.
• Pea 100, UF—an ultra-filtered peptone digested to enhance nucleoside and free amino acid content. The high total amino acid content and low carbohydrate content are similar to many high-performance animal origin peptones.
• Yeast 100—designed to enhance the content of vitamins and other key nutritional components found in yeast. It is intended for nutritional supplementation for mammalian, microbial, and insect cell culture.
Peptone Preview Pak supplements are non-GMP prototype products that are not for manufacture use. Larger sizes are available through the Gibco Custom Products Services.
Cotton 100, UF, is also part of the Peptone Preview Pak which contains:
• Cotton 200, UF—ultra-filtered and rich in nucleoside content, processed to enhance carbohydrate concentrations, which is important for some cell culture and microbial processes
• Wheat 100, UF—an ultra-filtered supplement and good source of glutamine, which makes it ideal for mammalian cell culture applications. It is digested for enhanced peptide content resulting in a low concentration of free amino acids and carbohydrates.
• Soy 100—a highly digested soy protein peptone that is high in nucleosides and free amino acid content. It has high concentrations of vitamins and carbohydrates and is recommend for a wide variety of processes.
• Pea 100, UF—an ultra-filtered peptone digested to enhance nucleoside and free amino acid content. The high total amino acid content and low carbohydrate content are similar to many high-performance animal origin peptones.
• Yeast 100—designed to enhance the content of vitamins and other key nutritional components found in yeast. It is intended for nutritional supplementation for mammalian, microbial, and insect cell culture.
Peptone Preview Pak supplements are non-GMP prototype products that are not for manufacture use. Larger sizes are available through the Gibco Custom Products Services.
Cotton Peptone 200, ultra-filtered (UF) Gibco™
Cotton 200, UF, is rich in nucleoside content and digested to enhance peptide content. It is processed to provide higher concentrations of vitamins, which can help maintain performance in mammalian and microbial processes.
Cotton 200, UF, is also part of the Peptone Preview Pak which contains:
• Cotton 100, UF—rich in nucleoside content and digested to enhance peptide content. It is processed to provide higher concentrations of vitamins, which can help maintain performance in mammalian and microbial processes.
• Wheat 100, UF—ultra-filtered supplement and good source of glutamine, which makes it ideal for mammalian cell culture applications. It is digested for enhanced peptide content resulting in a low concentration of free amino acids and carbohydrates.
• Soy 100—highly digested soy protein peptone that is high in nucleosides and free amino acid content. It has high concentrations of vitamins and carbohydrates and is recommend for a wide variety of processes.
• Pea 100, UF—ultra-filtered peptone digested to enhance nucleoside and free amino acid content. The high total amino acid content and low carbohydrate content are similar to many high-performance animal origin peptones.
• Yeast 100—designed to enhance the content of vitamins and other key nutritional components found in yeast. It is intended for nutritional supplementation for mammalian, microbial, and insect cell culture.
Peptone Preview Pak supplements are non-GMP prototype products that are not for manufacture use. Larger sizes are available through the Gibco Custom Products Services.
L-Glutamine (200 mM) Gibco™
L-Glutamine is an amino acid that is required for cell culture. L-Glutamine participates in the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, and other amino acids, as well as in protein synthesis and glucose production. Unlike most other amino acids, L-glutamine is not stable in solution. The rate at which degradation proceeds is a function of time, temperature, and pH. Gibco® L-glutamine is a ready-to-use 200 mM stock solution that acts as a cell culture supplement. The optimal concentration is dependent upon the cell type and medium used to culture the cells, but generally falls in the range of 2–6 mM.
We also offer Gibco® GlutaMAX™ Supplement as a stable alternative to L-glutamine.
Dual-site cGMP Manufacturing and Quality System
Gibco® L-glutamine is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® L-glutamine product made in our Grand Island facility (25030-081). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
We also offer Gibco® GlutaMAX™ Supplement as a stable alternative to L-glutamine.
Dual-site cGMP Manufacturing and Quality System
Gibco® L-glutamine is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® L-glutamine product made in our Grand Island facility (25030-081). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Tryptose Phosphate Broth
Gibco® Tryptose Phosphate Broth is a cell culture supplement for BHK-21 cells used for vaccine production. Tryptose Phosphate Broth contains tryptose (an enzymatic hydrolysis of protein), dextrose, sodium chloride and disodium phosphate.
Product Use
For Research Use Only. Not for use in diagnostic procedures.
cGMP Manufacturing and Quality System
Gibco® Tryptose Phosphate Broth is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Product Use
For Research Use Only. Not for use in diagnostic procedures.
cGMP Manufacturing and Quality System
Gibco® Tryptose Phosphate Broth is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
StemPro™ Accutase™ Cell Dissociation Reagent Gibco™
StemPro® Accutase® Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. Accutase® does not contain mammalian or bacterial derived products and is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex™, CELLStart™ and polymers. Accutase® performs exceptionally well in detaching primary and stem cells and maintaining high cell viabilty compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase® treatment include analysis of cell surface markers, virus growth assay, cell proliferation, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry. Cell lines tested for Accutase® application include human embryonic stem cells, human mesenchymal stem cells, human neural stem cells and primary cells including macrophages, fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, and established cell lines such as adherent CHO and BHK cells and 293 cells. A complete list of published references is available on our website. Accutase® is a trademark of ICT, Inc.
Anti-Clumping Agent Gibco™
GIBCO® Anti-Clumping Agent is a concentrated liquid reagent that reduces cell clumping to assist in attaining higher viable cell densities in suspension culture. Anti-Clumping Agent is a gentle, animal origin-free, chemically defined formulation that contains no proteins, enzymes, hydrolysates, or components of unknown composition.
• Reduced cell clumping in suspension culture
• Higher viable cell densities
• Animal origin-free and chemically defined components
• Convenient packaging with a larger size now available
• Customization available to configure packaging and delivery
Simple and Gentle Cell Dissociation
Cell lines adapted to suspension culture can revert to a more adherent or aggregated “clumping" state. When this occurs, culture condition can be greatly improved by adding a gentle reagent to reduce clumping and adhesion instead of the harsh enzymes often found in cell dissociation products. Anti-Clumping Agent is a simple, gentle, concentrated solution which, when added to culture media (usually titrated between a 1:100 and 1:1000 dilution), can reduce cell clumping significantly.
Reduced Clumping, Increased Viability, Improved Protein Expression
Reduced cell clumping achieved through use of Anti-Clumping Agent is illustrated below (Fig 1). See also the data demonstrating improved viable cell density (Fig 2). Higher viable cell density contributes to the stabilization of culture conditions for better protein yield.
For actual cell dissociation, we recommend our animal origin-free TrypLE™ products.
Use Anti-Clumping Agent after Transfection
Anti-Clumping Agent is not recommended in all suspension cell culture conditions. For example, when creating a cell line using lipid cationic transfection, Anti-Clumping Agent can interfere with the transfection process, so in this case we recommend that Anti-Clumping Agent be used after transfection.
Animal Origin-free and Chemically Defined Components
Because Anti-Clumping Agent is made with only animal origin free and chemically defined components, you can be assured that the risk of adding adventitious agents to your culture system is minimized. Avoiding additives in your system that are not well defined can lead to more robust results from batch to batch.
Convenient and Customizable Packaging
Previously, Anti-Clumping Agent was only available in a 20 mL bottle. However, it is now available in a more economical and convenient 100 mL bottle. For even larger packaging, you can custom configure this product by clicking on the 'Customize this Product' link under the 'Add to Cart' button above or by emailing us at CustomMedia@lifetech.com.
Additional Information and Support
Please review the Manuals and Protocols provided below for additional information on successful use of this product. Direct any unanswered questions to our Technical Services group.
For Research Use Only. Not for use in diagnostics procedures.
• Reduced cell clumping in suspension culture
• Higher viable cell densities
• Animal origin-free and chemically defined components
• Convenient packaging with a larger size now available
• Customization available to configure packaging and delivery
Simple and Gentle Cell Dissociation
Cell lines adapted to suspension culture can revert to a more adherent or aggregated “clumping" state. When this occurs, culture condition can be greatly improved by adding a gentle reagent to reduce clumping and adhesion instead of the harsh enzymes often found in cell dissociation products. Anti-Clumping Agent is a simple, gentle, concentrated solution which, when added to culture media (usually titrated between a 1:100 and 1:1000 dilution), can reduce cell clumping significantly.
Reduced Clumping, Increased Viability, Improved Protein Expression
Reduced cell clumping achieved through use of Anti-Clumping Agent is illustrated below (Fig 1). See also the data demonstrating improved viable cell density (Fig 2). Higher viable cell density contributes to the stabilization of culture conditions for better protein yield.
For actual cell dissociation, we recommend our animal origin-free TrypLE™ products.
Use Anti-Clumping Agent after Transfection
Anti-Clumping Agent is not recommended in all suspension cell culture conditions. For example, when creating a cell line using lipid cationic transfection, Anti-Clumping Agent can interfere with the transfection process, so in this case we recommend that Anti-Clumping Agent be used after transfection.
Animal Origin-free and Chemically Defined Components
Because Anti-Clumping Agent is made with only animal origin free and chemically defined components, you can be assured that the risk of adding adventitious agents to your culture system is minimized. Avoiding additives in your system that are not well defined can lead to more robust results from batch to batch.
Convenient and Customizable Packaging
Previously, Anti-Clumping Agent was only available in a 20 mL bottle. However, it is now available in a more economical and convenient 100 mL bottle. For even larger packaging, you can custom configure this product by clicking on the 'Customize this Product' link under the 'Add to Cart' button above or by emailing us at CustomMedia@lifetech.com.
Additional Information and Support
Please review the Manuals and Protocols provided below for additional information on successful use of this product. Direct any unanswered questions to our Technical Services group.
For Research Use Only. Not for use in diagnostics procedures.
CTS™ TrypLE™ Select Enzyme Gibco™
TrypLE™ Select CTS™, a non-animal alternative for porcine Trypsin, is a recombinant enzyme derived from microbial fermentation. The GIBCO® CTS™ product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications.
•GIBCO® TrypLE™ Select ™ CTS™ enables you to reduce your burden in qualifying reagents during your transition from research to clinical applications.
•Non-animal or human origin
•Reduced risk of viral or prion contamination
•Removal kinetics and recovery viability comparable to porcine trypsin
•Demonstration of activity on multiple cell lines, including strongly attached lines
•Stable at room temperature (15 to 30°C) for up to 24 months.
•Manufactured using dedicated, autoclaved or disposable equipment.
TrypLE™ Select CTS™, a non-animal alternative for porcine Trypsin, is a recombinant enzyme derived from microbial fermentation. This material is used for the dissociation of attachment-dependent cell lines from plasticware. TrypLE Select has demonstrated the ability to dissociate cells cultured both in serum-free and serum supplemented systems. The product is provided as a 1X stock solution, formulated in DPBS with 1mM EDTA. The concentration of rProtease is proprietary to
Invitrogen Corp.
GIBCO® CTS™ products are of high quality, and are supplied with harmonized documentation such as Certificates of Analysis, Certificates of Origin, and access to authorization letters for our Drug Master Files, as appropriate. The GIBCO® CTS™ product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications.
For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals
•GIBCO® TrypLE™ Select ™ CTS™ enables you to reduce your burden in qualifying reagents during your transition from research to clinical applications.
•Non-animal or human origin
•Reduced risk of viral or prion contamination
•Removal kinetics and recovery viability comparable to porcine trypsin
•Demonstration of activity on multiple cell lines, including strongly attached lines
•Stable at room temperature (15 to 30°C) for up to 24 months.
•Manufactured using dedicated, autoclaved or disposable equipment.
TrypLE™ Select CTS™, a non-animal alternative for porcine Trypsin, is a recombinant enzyme derived from microbial fermentation. This material is used for the dissociation of attachment-dependent cell lines from plasticware. TrypLE Select has demonstrated the ability to dissociate cells cultured both in serum-free and serum supplemented systems. The product is provided as a 1X stock solution, formulated in DPBS with 1mM EDTA. The concentration of rProtease is proprietary to
Invitrogen Corp.
GIBCO® CTS™ products are of high quality, and are supplied with harmonized documentation such as Certificates of Analysis, Certificates of Origin, and access to authorization letters for our Drug Master Files, as appropriate. The GIBCO® CTS™ product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications.
For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals
Neomycin Sulfate Gibco™
Gibco® Neomycin is produced by Streptomyces fradiae. Polymyxin B Sulfate acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Gibco® Neomycin Sulfate is used for the prevention of bacterial contamination of cell cultures. This antibiotic is highly active against gram-negative bacteria. The recommended working concentration is 50 µg/ml. We offer a variety of antibiotics and antimycotics for cell culture applications.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
Phytone™ Peptone Gibco™
Phytone Peptone is an animal origin-free (AOF) enzymatic digest of soy. Due to the naturally high concentrations of vitamins and carbohydrates in soy, Phytone Peptone is a nutritious and excellent carbohydrate source. Customers have successfully used this supplement in CHO-based production of monoclonal antibodies for oncological and rheumatological therapeutics, as well as with many different microbial cell types to produce animal health vaccines for swine, such as Clostridial vaccines.
Features:
• Animal origin-free (AOF) supplement
• Enzymatic digests of soybean meal/flour
• Retains the high vitamin and high carbohydrate content of the soy plant tissue
Applications of Phytone Peptone include:
• It is an excellent plant peptone for the cultivation of fungi and fastidious types of bacteria, such as members of the Clostridium and Neisseria genera
• It has been used in mammalian cell culture applications due to its high carbohydrate content
Features:
• Animal origin-free (AOF) supplement
• Enzymatic digests of soybean meal/flour
• Retains the high vitamin and high carbohydrate content of the soy plant tissue
Applications of Phytone Peptone include:
• It is an excellent plant peptone for the cultivation of fungi and fastidious types of bacteria, such as members of the Clostridium and Neisseria genera
• It has been used in mammalian cell culture applications due to its high carbohydrate content
KaryoMAX™ Giemsa Stain Solution Gibco™
Gibco® KaryoMAX® Giemsa Stain Solution is a solution used for G-banding of chromosomes for cytogenetic analysis. Gibco® KaryoMAX® Giemsa Stain Solution is formulated as 6.0 g Azur II Eosin and 1.6 g Azur II per liter in glycerol/methanol.
Product Use
For in vitro diagnostic use.
cGMP Manufacturing and Quality System
Gibco® KaryoMAX® Giemsa Stain Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Product Use
For in vitro diagnostic use.
cGMP Manufacturing and Quality System
Gibco® KaryoMAX® Giemsa Stain Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
EfficientFeed™ A+ 3X Supplement Gibco™
Gibco EfficientFeed A+ 3X Supplement is a single-part liquid supplement for fed batch addition to the culture of multiple cell lines to assist with process development and maximization of bioreactor utilization. Compared to original CHO CD EfficientFeed A AGT nutrient supplement, EfficientFeed A+ 3X Supplement has components that are super-concentrated from 66.4 g/L (1X AGT) to 199.2 g/L (3X), helping reduce product dilution or increase titers with additional feeding. The supplement is an animal origin-free, chemically defined formulation that contains no proteins, hydrolysates, or components of incompletely defined composition.
Which EfficientFeed+ supplement to choose?
Consider EfficientFeed A+ Supplement when:
• You have used CHO CD EfficientFeed A supplement in the past and wish to explore simpler reconstitution and super-concentration benefits during delivery of key components
• You are currently using CD OptiCHO as your base cell growth medium
Consider EfficientFeed B+ Supplement when:
• You have used CHO CD EfficientFeed B supplement in the past and wish to explore simpler reconstitution and super-concentration benefits during delivery of key components
• You are currently using CD CHO as your base cell growth medium
Consider EfficientFeed C+ Supplement when:
• You have used CD EfficientFeed C supplement in the past and wish to explore super-concentration benefits during delivery of key components
• You want higher productivity in GS CHO, CHO-K1, and CHO-S derived cell lines
• You are currently using CD FortiCHO as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at Day 0
Increasing titers
As a complete feed, EfficientFeed+ supplements have been shown to provide multiple-fold increases in titer versus batch culture and are an excellent starting point for process development as ready-to-use single-part liquid feeds.
Maximizing bioreactor utilization
Have you ever wished for more working volume at the start of a bioreactor run to produce more protein, but need too much feeding volume space? Have you ever thought that room for more feeding might achieve a new level of titer from a process and maybe even reduce the number of bioreactor runs needed per year? Using super-concentrated ready-to-feed liquid feeding supplements helps reduce product dilution, increase space for additional feeding, and/or increase the working volume at the start of the run. In fact, you may gain about 20% more bioreactor utilization with this super-concentrated EfficientFeed+ product.
Simplified handling to reduce risk in scale-up
The ability to scale up in liquid or AGT dry format provides ease of handling and storage in manufacturing and other bioprocess settings. Fewer parts and steps in reconstitution mean fewer opportunities for operator error. Further, the ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature, in the dark, helps reduce chances of contamination during closed system connections by reducing the number of connections to the bioreactor to one.
Which EfficientFeed+ supplement to choose?
Consider EfficientFeed A+ Supplement when:
• You have used CHO CD EfficientFeed A supplement in the past and wish to explore simpler reconstitution and super-concentration benefits during delivery of key components
• You are currently using CD OptiCHO as your base cell growth medium
Consider EfficientFeed B+ Supplement when:
• You have used CHO CD EfficientFeed B supplement in the past and wish to explore simpler reconstitution and super-concentration benefits during delivery of key components
• You are currently using CD CHO as your base cell growth medium
Consider EfficientFeed C+ Supplement when:
• You have used CD EfficientFeed C supplement in the past and wish to explore super-concentration benefits during delivery of key components
• You want higher productivity in GS CHO, CHO-K1, and CHO-S derived cell lines
• You are currently using CD FortiCHO as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at Day 0
Increasing titers
As a complete feed, EfficientFeed+ supplements have been shown to provide multiple-fold increases in titer versus batch culture and are an excellent starting point for process development as ready-to-use single-part liquid feeds.
Maximizing bioreactor utilization
Have you ever wished for more working volume at the start of a bioreactor run to produce more protein, but need too much feeding volume space? Have you ever thought that room for more feeding might achieve a new level of titer from a process and maybe even reduce the number of bioreactor runs needed per year? Using super-concentrated ready-to-feed liquid feeding supplements helps reduce product dilution, increase space for additional feeding, and/or increase the working volume at the start of the run. In fact, you may gain about 20% more bioreactor utilization with this super-concentrated EfficientFeed+ product.
Simplified handling to reduce risk in scale-up
The ability to scale up in liquid or AGT dry format provides ease of handling and storage in manufacturing and other bioprocess settings. Fewer parts and steps in reconstitution mean fewer opportunities for operator error. Further, the ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature, in the dark, helps reduce chances of contamination during closed system connections by reducing the number of connections to the bioreactor to one.
Resurge™ CD Pak Gibco™
Resurge CD Pak are a diverse set of chemically defined supplement formulations to complement a variety of base media. The CD PAK contains one of each Resurge supplement: CD1, CD2, CD3, CD4, and CD5 at 100 grams each. The supplements have been developed using several host cell lines and in various culture systems, including shaker flasks and bench-scale bioreactors. In order to increase versatility of the formulation, glucose and L-glutamine are not included in the final formulations. Therefore, these two components should be added by users and monitored as appropriate.
Difco™ TC Yeastolate, ultra-filtered (UF) Gibco™
Difco TC Yeastolate, ultra-filtered (UF), is an animal origin-free (AOF) product derived from water-soluble portions of autolyzed yeast (Saccharomyces cerevisiae). This product is intended as a nutritional supplement for bacterial, insect, and mammalian cell culture.
Gentamicin/Amphotericin Solution Gibco™
A sterile, concentrated (500×) antibiotic⁄antimycotic solution of gentamicin and amphotericin B (10 × 1 ml vials⁄pkg). One Gentamicin⁄Amphotericin Solution 10-Pak is enough to supplement ten 500 ml bottles of basal medium. Animal origin-free.
Bacto™ Peptone Gibco™
Bacto Peptone is an animal origin (AO) enzymatic digest of bovine and porcine animal proteins. The nutritive value of Bacto Peptone is largely dependent on a combination of a negligible quantity of proteases, high concentration of complex constituents, and a unique amino acid content that makes this peptone a strong nitrogen source. Customers have successfully used this supplement with a variety of cell types including Saccharomyces cerevisiae, Escherichia coli, Pasteurella multocida, Vibrio cholerae, and porcine kidney cells to produce biotherapeutics and vaccines for animal health applications.
Features:
• A strong nitrogen source
• Unique amino acid content
• High concentration of complex constituents
Applications of Bacto Peptone include:
• Vaccines for animal health applications
• Production of biotherapeutics
• Mammalian cell culture
• Microbial fermentation
Features:
• A strong nitrogen source
• Unique amino acid content
• High concentration of complex constituents
Applications of Bacto Peptone include:
• Vaccines for animal health applications
• Production of biotherapeutics
• Mammalian cell culture
• Microbial fermentation
