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Acidicase™ Peptone Gibco™
Acidicase Peptone is a hydrochloric acid hydrolysate of casein. The manufacturing process produces a casein hydrolysate, which has a high salt content of approximately 37% and nitrogen content of approximately 8%. The hydrolysis of the casein, a milk protein rich in amino acid nitrogen, is carried out until all the nitrogen is converted to amino acids or other compounds of relative simplicity. Casein contains little cystine and is deficient in tryptophan, which is destroyed by the acid treatment.
Features:
• Hydrochloric acid hydrolysate of casein
• Animal origin (AO) nutritional supplement
• Milk protein rich in amino acid nitrogen
Applications of Acidicase Peptone include:
• Intended for use as a nutritional supplement in vitamin assays, susceptibility testing, and other laboratory media and microbial fermentation in which the high salt content will not interfere
Features:
• Hydrochloric acid hydrolysate of casein
• Animal origin (AO) nutritional supplement
• Milk protein rich in amino acid nitrogen
Applications of Acidicase Peptone include:
• Intended for use as a nutritional supplement in vitamin assays, susceptibility testing, and other laboratory media and microbial fermentation in which the high salt content will not interfere
Cryopreserved Hepatocyte Plating Medium (CHPM) Gibco™
Cryopreserved Hepatocyte Plating Medium (CHPM) used for plating Gibco® cryopreserved human hepatocytes for optimal attachment and monolayer formation.
For research use only. Not intended for human or animal therapeutic or diagnostic use.
Related Link
Learn more about our Hepatic Cell Culture reagents
For research use only. Not intended for human or animal therapeutic or diagnostic use.
Related Link
Learn more about our Hepatic Cell Culture reagents
Bacto™ Peptone Gibco™
Bacto Peptone is an animal origin (AO) enzymatic digest of bovine and porcine animal proteins. The nutritive value of Bacto Peptone is largely dependent on a combination of a negligible quantity of proteases, high concentration of complex constituents, and a unique amino acid content that makes this peptone a strong nitrogen source. Customers have successfully used this supplement with a variety of cell types including Saccharomyces cerevisiae, Escherichia coli, Pasteurella multocida, Vibrio cholerae, and porcine kidney cells to produce biotherapeutics and vaccines for animal health applications.
Features:
• A strong nitrogen source
• Unique amino acid content
• High concentration of complex constituents
Applications of Bacto Peptone include:
• Vaccines for animal health applications
• Production of biotherapeutics
• Mammalian cell culture
• Microbial fermentation
Features:
• A strong nitrogen source
• Unique amino acid content
• High concentration of complex constituents
Applications of Bacto Peptone include:
• Vaccines for animal health applications
• Production of biotherapeutics
• Mammalian cell culture
• Microbial fermentation
GlutaMAX™ Supplement Thermo Scientific™
Gibco® GlutaMAX™ Supplement is an alternative to L-glutamine, with increased stability that improves cell health. GlutaMAX™ Supplement is suitable for both adherent and suspension culture of mammalian cells, with no adaptation required. GlutaMAX™ Supplement is offered as a 200 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl. GlutaMAX™ Supplement is also included in a variety of media formulations. Compared to L-glutamine, GlutaMAX™ Supplement:
Features:
• Minimizes toxic ammonia build-up
• Improves cell viability and growth
• Remains stable across a wide range of temperatures
Minimizes toxic ammonia build-up
Unlike L-glutamine, GlutaMAX™ Supplement does not spontaneously break down to form ammonia. Instead, cells cleave the dipeptide bond to release L-glutamine as needed. This system prevents waste build-up and maintains a fresh supply of L-glutamine during long-term culture.
Improves cell viability and growth
Cultures with lower levels of ammonia show improved cell viability. In addition, culture conditions are more controlled due to the consistent concentration of GlutaMAX™ Supplement that is not possible with L-glutamine.
Remains stable across a wide range of temperatures
GlutaMAX™ Supplement is room-temperature stable and ready to use. GlutaMAX™ Supplement retains full functionality for 24 months at room temperature, 2 to 8°C, or -5 to -30°C for convenient storage and handling. GlutaMAX™ Supplement is even stable at 37°C for a week or more.
cGMP manufacturing and quality system
Gibco® GlutaMAX™ supplement is manufactured at a cGMP compliant facility, located in Paisley, Scotland. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® GlutaMAX™ supplement made in our Grand Island, New York facility (35050-061). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Features:
• Minimizes toxic ammonia build-up
• Improves cell viability and growth
• Remains stable across a wide range of temperatures
Minimizes toxic ammonia build-up
Unlike L-glutamine, GlutaMAX™ Supplement does not spontaneously break down to form ammonia. Instead, cells cleave the dipeptide bond to release L-glutamine as needed. This system prevents waste build-up and maintains a fresh supply of L-glutamine during long-term culture.
Improves cell viability and growth
Cultures with lower levels of ammonia show improved cell viability. In addition, culture conditions are more controlled due to the consistent concentration of GlutaMAX™ Supplement that is not possible with L-glutamine.
Remains stable across a wide range of temperatures
GlutaMAX™ Supplement is room-temperature stable and ready to use. GlutaMAX™ Supplement retains full functionality for 24 months at room temperature, 2 to 8°C, or -5 to -30°C for convenient storage and handling. GlutaMAX™ Supplement is even stable at 37°C for a week or more.
cGMP manufacturing and quality system
Gibco® GlutaMAX™ supplement is manufactured at a cGMP compliant facility, located in Paisley, Scotland. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® GlutaMAX™ supplement made in our Grand Island, New York facility (35050-061). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Gentamicin (10 mg/mL) Gibco™
Gentamicin sulfate is a water-soluble antibiotic drug originally purified from the fungus Micromonospora purpurea. Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition protein synthesis and death in susceptible bacteria. Gibco® Gentamicin is effective against a wide variety of gram-positive and gram-negative bacteria, and is used for the prevention of bacterial contamination on cell cultures. The recommended working concentration ranges from 0.5 to 50 µg/ml. We offer a variety of antibiotics and antimycotics for cell culture applications.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
Dual-Site cGMP Manufacturing
Gibco® Gentamicin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® Gentamicin product made in our Scotland facility (15710-049). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
Dual-Site cGMP Manufacturing
Gibco® Gentamicin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® Gentamicin product made in our Scotland facility (15710-049). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Gurr Buffer Tablets Gibco™
Gibco® Gurr Buffer Tablets offer a convenient way to prepare a pH 6.8 phosphate buffer for dilution of Giemsa stain used in G-banding of chromosomes for cytogenetic analysis. Simply add one buffer tablet to one liter of distilled water and stir until dissolved.
Ampicillin, sodium salt, irradiated Gibco™
Ampicillin is an antibiotic in the broad-spectrum penicillin group. Ampicillin differs from penicillin only by the presence of an amino group, which facilitates penetration through the outer membrane of some gram-negative bacteria. Ampicillin acts by interfering directly with the turnover of the bacterial cell wall and indirectly by triggering the release of enzymes that further alter the cell wall.
Gibco Ampicillin is used as a selective antibiotic generally at a concentration of 20–125 µg/mL. Ampicillin selection is based on hydrolysis and inactivation of its beta-lactam ring by beta-lactamase expressed by the bla gene. Sometimes, beta-lactamase produced by the bacteria is secreted into the medium resulting in inactivation of ampicillin in the culture medium. Ampicillin degradation on agar plates can lead to the formation of satellite colonies. Growth of these colonies can be halted by adding fresh ampicillin. To avoid beta-lactamase buildup, special care must be taken while handling cultures and determining the correct dose of antibiotic.
Ampicillin antibiotic in powder form is an economical choice that can be used in a wide range of gram-positive and gram-negative infections. This product is provided as a powder and should be made into a stock solution at a concentration of 10 mg/mL in water.
cGMP manufacturing
Gibco Ampicillin is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Gibco Ampicillin is used as a selective antibiotic generally at a concentration of 20–125 µg/mL. Ampicillin selection is based on hydrolysis and inactivation of its beta-lactam ring by beta-lactamase expressed by the bla gene. Sometimes, beta-lactamase produced by the bacteria is secreted into the medium resulting in inactivation of ampicillin in the culture medium. Ampicillin degradation on agar plates can lead to the formation of satellite colonies. Growth of these colonies can be halted by adding fresh ampicillin. To avoid beta-lactamase buildup, special care must be taken while handling cultures and determining the correct dose of antibiotic.
Ampicillin antibiotic in powder form is an economical choice that can be used in a wide range of gram-positive and gram-negative infections. This product is provided as a powder and should be made into a stock solution at a concentration of 10 mg/mL in water.
cGMP manufacturing
Gibco Ampicillin is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Bacto™ Neopeptone Gibco™
Bacto Neopeptone is an animal origin (AO) enzymatic digest of bovine (milk) and porcine protein. This nutritional supplement provides vitamins, nucleotides, minerals, and peptides in a variety of sizes to any culture formulation. Customers have used Bacto Neopeptone as a component of culture media in the cultivation of human pathogens, notably Bordetella pertussis and group A streptococci, as well as Borrelia burgdorferi and Clostridium species, to produce vaccines for animal health applications.
Features:
• Enzymatic digest of bovine (milk) and porcine protein
• Animal origin nutritional supplement
• Contains a wide variety of peptide sizes in combination with vitamins, nucleotides, and minerals
Applications of Bacto Neopeptone include:
• Recommended for use in media for detection of fungi
• Reported to provide nutrients for support of spirochetes and protozoa
• Microbial fermentation
• Production of vaccines for animal health applications
Features:
• Enzymatic digest of bovine (milk) and porcine protein
• Animal origin nutritional supplement
• Contains a wide variety of peptide sizes in combination with vitamins, nucleotides, and minerals
Applications of Bacto Neopeptone include:
• Recommended for use in media for detection of fungi
• Reported to provide nutrients for support of spirochetes and protozoa
• Microbial fermentation
• Production of vaccines for animal health applications
GlutaMAX™ Supplement Gibco™
Gibco® GlutaMAX™ Supplement is an alternative to L-glutamine, with increased stability that improves cell health. GlutaMAX™ Supplement is suitable for both adherent and suspension culture of mammalian cells, with no adaptation required. GlutaMAX™ Supplement is offered as a 200 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl. GlutaMAX™ Supplement is also included in a variety of media formulations. Compared to L-glutamine, GlutaMAX™ Supplement:
• Minimizes toxic ammonia build-up
• Improves cell viability and growth
• Remains stable across a wide range of temperatures
Minimizes toxic ammonia build-up
Unlike L-glutamine, GlutaMAX™ Supplement does not spontaneously break down to form ammonia. Instead, cells cleave the dipeptide bond to release L-glutamine as needed. This system prevents waste build-up and maintains a fresh supply of L-glutamine during long-term culture.
Improves cell viability and growth
Cultures with lower levels of ammonia show improved cell viability. In addition, culture conditions are more controlled due to the consistent concentration of GlutaMAX™ Supplement that is not possible with L-glutamine.
Remains stable across a wide range of temperatures
GlutaMAX™ Supplement is room-temperature stable and ready to use. GlutaMAX™ Supplement retains full functionality for 24 months at room temperature, 2 to 8°C, or -5 to -30°C for convenient storage and handling. GlutaMAX™ Supplement is even stable at 37°C for a week or more.
cGMP manufacturing and quality system
Gibco® GlutaMAX™ Supplement is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® PBS product made in our Scotland facility (35050-087). This facility is also registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
• Minimizes toxic ammonia build-up
• Improves cell viability and growth
• Remains stable across a wide range of temperatures
Minimizes toxic ammonia build-up
Unlike L-glutamine, GlutaMAX™ Supplement does not spontaneously break down to form ammonia. Instead, cells cleave the dipeptide bond to release L-glutamine as needed. This system prevents waste build-up and maintains a fresh supply of L-glutamine during long-term culture.
Improves cell viability and growth
Cultures with lower levels of ammonia show improved cell viability. In addition, culture conditions are more controlled due to the consistent concentration of GlutaMAX™ Supplement that is not possible with L-glutamine.
Remains stable across a wide range of temperatures
GlutaMAX™ Supplement is room-temperature stable and ready to use. GlutaMAX™ Supplement retains full functionality for 24 months at room temperature, 2 to 8°C, or -5 to -30°C for convenient storage and handling. GlutaMAX™ Supplement is even stable at 37°C for a week or more.
cGMP manufacturing and quality system
Gibco® GlutaMAX™ Supplement is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® PBS product made in our Scotland facility (35050-087). This facility is also registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Difco™ Beef Extract Gibco™
Difco Beef Extract is an animal origin peptone derived from an infusion of beef. This nutritional supplement provides a mixture of peptides, individual amino acids, nucleotides, organic acids, minerals, and vitamins. Provided as a paste, customers have used Difco Beef Extract in a variety of microbial fermentation cell lines, including Escherichia coli, Bacillus subtillis, Pasteurella multocida, and Streptococcus species to produce numerous animal health vaccines.
Features:
• An animal origin nutritional supplement
• A mixture of peptides, individual amino acids, nucleotides, organic acids, minerals, and vitamins
Applications of Difco Beef Extract include:
• Use in a variety of microbial fermentation cell lines
• Used to produce numerous animal health vaccines
Features:
• An animal origin nutritional supplement
• A mixture of peptides, individual amino acids, nucleotides, organic acids, minerals, and vitamins
Applications of Difco Beef Extract include:
• Use in a variety of microbial fermentation cell lines
• Used to produce numerous animal health vaccines
B-27™ Supplement, XenoFree, minus insulin Gibco™
B-27 Supplement XenoFree Minus Insulin is based on the classic B-27 Supplement formulation and contains no animal-derived components at the primary component level. The absence of insulin makes this formulation ideal for the growth of hippocampal and other CNS neurons in applications where the presence of insulin causes interference or in the derivation of cardiomyocytes and mesodermal cells from stem cells.
Quality
B-27 Supplement XenoFree Minus Insulin is manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485– and ISO 9001–certified.
Formulated xeno-free
The preparation contains no animal-derived components at the primary component level.
For other applications, we provide alternative formulations of B-27 supplements and Neurobasal media.
For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.
Quality
B-27 Supplement XenoFree Minus Insulin is manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485– and ISO 9001–certified.
Formulated xeno-free
The preparation contains no animal-derived components at the primary component level.
For other applications, we provide alternative formulations of B-27 supplements and Neurobasal media.
For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.
Insulin, human recombinant, zinc solution Gibco™
4 mg/ml
Potency: (dry basis) >= 27 USP units/mg.
Suggested range for supplementation: 2 to 20 µg/ml.
Potency: (dry basis) >= 27 USP units/mg.
Suggested range for supplementation: 2 to 20 µg/ml.
Bacto™ Yeast Extract, technical Gibco™
Bacto Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells. They are derived from primary grown baker’s yeast. These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications. Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation.
Bacto Yeast Extract is one of the most complete and versatile fermentation supplements available. It is an important ingredient for the microbiological assay of vitamins. Yeast extract is also of value in the assay of antibiotics. B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract. Bacto Yeast Extract, Technical, was developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics.
Related yeast extract products:
• Gibco Yeast Extract—developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Difco Yeast Extract UF—ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g.
• Difco Yeast Extract Low-Dusting (LD)—designed to limit the amount of dust generated compared to other spray-dried yeast extracts
Bacto Yeast Extract is one of the most complete and versatile fermentation supplements available. It is an important ingredient for the microbiological assay of vitamins. Yeast extract is also of value in the assay of antibiotics. B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract. Bacto Yeast Extract, Technical, was developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics.
Related yeast extract products:
• Gibco Yeast Extract—developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Difco Yeast Extract UF—ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g.
• Difco Yeast Extract Low-Dusting (LD)—designed to limit the amount of dust generated compared to other spray-dried yeast extracts
Bacto™ Soytone Gibco™
Bacto Soytone is an enzymatic digest of soy that uses an animal origin (AO) (porcine) enzyme for digestion. The naturally high concentrations of vitamins and carbohydrates found in soy make Bacto Soytone a nutritious and excellent carbohydrate source for those not concerned about the processing step involving an animal-based material. This supplement offers a unique nutritional profile as compared to other soy products, and so can be a valuable alternative to consider for supplementing both mammalian and microbial processes.
Staphylococcus aureus (Wood strain without protein A) BioParticles™, fluorescein conjugate Invitrogen™
The Molecular Probes® BioParticles® product line consists of a series of fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities. These fluorescent BioParticles® products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry.
We offer E. coli (K-12 strain), S. aureus (Wood strain, without protein A) and zymosan (S. cerevisiae) BioParticles® conjugates covalently labeled with a variety of different fluorophores (special care has been taken to remove free dye after conjugation). Unlike the fluorescence of fluorescein-labeled BioParticles® conjugates, which is partially quenched in acidic environments, the fluorescence of the Alexa Fluor®, BODIPY® FL, tetramethylrhodamine and Texas Red® dye conjugates is uniformly intense over the pH range from 3 to 10.
BioParticles Specifications:
• Label (Ex/Em): Fluorescein (~494/518 nm)
• Particle: S. aureus (Wood strain, without protein A)
• Opsonizing reagent available
Using BioParticles Products
BioParticles® conjugates are provided as lyophilized powders. There are approximately 3 x 108 E. coli or S. aureus particles per mg solid and approximately 2 x 107 zymosan particles per mg solid. BioParticles® conjugates can be reconstituted in the buffer of your choice for use in phagocytosis assays. The fluorescence of BioParticles® conjugates that are bound to the surface of the cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers. In addition to cellular applications, fluorescent BioParticles® conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants. These small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways.
Find More BioParticles® Products
We offer a large range of dye-labeled and unlabeled E. coli (K-12 strain), S. aureus (Wood strain, without protein A), and zymosan (S. cerevisiae) BioParticles® products. Find out about these products and their applications by reviewing Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook.
For pH-sensitive endocytosis assays, see our pHrodo™ BioParticles® conjugates.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
We offer E. coli (K-12 strain), S. aureus (Wood strain, without protein A) and zymosan (S. cerevisiae) BioParticles® conjugates covalently labeled with a variety of different fluorophores (special care has been taken to remove free dye after conjugation). Unlike the fluorescence of fluorescein-labeled BioParticles® conjugates, which is partially quenched in acidic environments, the fluorescence of the Alexa Fluor®, BODIPY® FL, tetramethylrhodamine and Texas Red® dye conjugates is uniformly intense over the pH range from 3 to 10.
BioParticles Specifications:
• Label (Ex/Em): Fluorescein (~494/518 nm)
• Particle: S. aureus (Wood strain, without protein A)
• Opsonizing reagent available
Using BioParticles Products
BioParticles® conjugates are provided as lyophilized powders. There are approximately 3 x 108 E. coli or S. aureus particles per mg solid and approximately 2 x 107 zymosan particles per mg solid. BioParticles® conjugates can be reconstituted in the buffer of your choice for use in phagocytosis assays. The fluorescence of BioParticles® conjugates that are bound to the surface of the cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers. In addition to cellular applications, fluorescent BioParticles® conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants. These small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways.
Find More BioParticles® Products
We offer a large range of dye-labeled and unlabeled E. coli (K-12 strain), S. aureus (Wood strain, without protein A), and zymosan (S. cerevisiae) BioParticles® products. Find out about these products and their applications by reviewing Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook.
For pH-sensitive endocytosis assays, see our pHrodo™ BioParticles® conjugates.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
