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Escherichia coli (K-12 strain) BioParticles™, Texas Red™ conjugate Invitrogen™

The Molecular Probes® BioParticles® product line consists of a series of fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities. These fluorescent BioParticles® products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry.

We offer E. coli (K-12 strain), S. aureus (Wood strain, without protein A) and zymosan (S. cerevisiae) BioParticles® conjugates covalently labeled with a variety of different fluorophores (special care has been taken to remove free dye after conjugation). Unlike the fluorescence of fluorescein-labeled BioParticles® conjugates, which is partially quenched in acidic environments, the fluorescence of the Alexa Fluor®, BODIPY® FL, tetramethylrhodamine and Texas Red® dye conjugates is uniformly intense over the pH range from 3 to 10.

BioParticles Specifications:
• Label (Ex/Em): Texas Red® (~595/615 nm)
• Particle: E. coli (K-12 strain)
Opsonizing reagent available


Using BioParticles Products
BioParticles® conjugates are provided as lyophilized powders. There are approximately 3 x 108 E. coli or S. aureus particles per mg solid and approximately 2 x 107 zymosan particles per mg solid. BioParticles® conjugates can be reconstituted in the buffer of your choice for use in phagocytosis assays. The fluorescence of BioParticles® conjugates that are bound to the surface of the cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers. In addition to cellular applications, fluorescent BioParticles® conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants. These small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways.

Find More BioParticles® Products
We offer a large range of dye-labeled and unlabeled E. coli (K-12 strain), S. aureus (Wood strain, without protein A), and zymosan (S. cerevisiae) BioParticles® products. Find out about these products and their applications by reviewing Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook.

For pH-sensitive endocytosis assays, see our pHrodo™ BioParticles® conjugates.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Trypsin/EDTA Solution (TE) Gibco™

Gibco® Trypsin solutions are made from trypsin powder - a mixture of proteases derived from bovine pancreas. Due to its digestive strength, it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation. This Gibco® Trypsin/EDTA solution is formulation as 0.025% trypsin and 0.01% EDTA in Phosphate Buffered Saline (PBS). We offer a variety of Gibco® Trypsin and animal origin free TrypLE™ cell dissociation products to meet your needs.

This trypsin is manufactured as follows:

With
EDTA
Phenol Red
The complete formulation is available.

Product Use
For Research Use Only. Not for use in diagnostic procedures.

cGMP Manufacturing and Quality System
Gibco® Trypsin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Escherichia coli BioParticles™ Opsonizing Reagent Invitrogen™

The Escherichia coli BioParticles® opsonizing agent is derived from highly purified rabbit polyclonal IgG antibodies and specifically designed to enhance the uptake of the Escherichia coli BioParticals® conjugates.

Accutase - Enzyme Cell Detachment Medium Invitrogen™

Accutase performs exceptionally well in detaching cells for analysis of cell surface markers, virus growth assay, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, cell haptotaxsis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry. Accutase has been shown effective on: fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.

Reported Application
Cell Culture

Trypsin Neutralizer Solution Gibco™

A sterile, phosphate-buffered saline solution (1X) containing calf serum as a trypsin inhibitor.

Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 647 Streptavidin (Alexa Fluor™ 647 EGF complex) Invitrogen™

We offer several conjugates of epidermal growth factor that are useful for the detection of EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. The conjugates incorporating fluorescein (FITC), Oregon Green® 514, and tetramethylrhodamine were constructed with the dye directly attached to EGF. The conjugates of EGF with Alexa Fluor® dyes and Texas Red® dye are complexes of the dye–streptavidin molecule and biotinylated EGF. The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe’s affinity for the EGF receptor.

Epidermal Growth Factor Conjugate Specifications:
• EGF molecule: 53 amino acids, MW=6,045 Da
• Label (Ex/Em): Biotin-XX complexed with Alexa Fluor® 647 streptavidin (~650/665 nm)
• Number of fluorophore molecules on each EGF molecule: ~2–3
• Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter


Find More Receptor Binding and Phagocytosis Probes
We offer a number of fluorescently labeled probes for studying receptor-mediated endocytosis, membrane markers for endocytosis and exocytosis, and methods for detecting internalized fluorescent ligands. Review Probes for Following Receptor Binding and Phagocytosis—Section 16.1 in the Molecular Probes® Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.

HuMEC Supplement Kit Gibco™

HuMEC Supplement Kit contains growth factors and other components and is designed to work with HuMEC Basal Serum-Free Medium (12753018). Together, these products are engineered specifically for the culture of human mammary epithelial cells (A10565). This translates to greater experimental validity, more reproducible results, and reduction of lot-to-lot variability and aberrations that can occur with the addition of serum. Human mammary epithelial cells cultured in HuMEC Medium have gene expression profiles consistent with normal mammary epithelial cells, as tested on a cDNA microarray.

Applications:
HuMEC Medium is optimized specifically for the growth of human mammary epithelial cells, and has been performance tested in a growth assay using normal human mammary epithelial cells. HuMEC Medium promotes superior growth of normal human mammary epithelial cells, making it ideal for researchers in breast cancer, drug discovery, and other aspects of breast biology.

Contents and Storage:
The HuMEC Supplement Kit includes 5 ml of a supplement mix containing epidermal growth factor, hydrocortisone, isoproterenol, transferrin, and insulin, and 25 mg of bovine pituitary extract. Store the supplement mix at -5°C to -20°C.

KaryoMAX™ Colcemid™ Solution in HBSS Gibco™

Gibco® KaryoMAX® Colcemid™ Solution in HBSS is a 10 µg/mL N-desacetyl-N-methylocolchicine (Colcemid™) solution made up in Hanks' Balanced Salt Solution (HBSS). Colcemid™ prevents spindle formation during mitosis, arresting cells in metaphase so that the chromosomes can be separated for cytogenetic studies and in vitro diagnostic procedures. The mechanism of action is similar to that of colchicine, but with lower mammalian toxicity.

The complete formulation is available.

cGMP Manufacturing and Quality System
Gibco® KaryoMAX® Colcemid™ Solution in HBSS is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Difco™ Soytone Gibco™

Difco Soytone is an enzymatic digest of soy. With high concentrations of vitamins and carbohydrates uniquely present in soy, this supplement offers an alternative carbohydrate profile to yeast supplements for mammalian cell processes, such as CHO.

Features:
• An enzymatic digest of soy
• High concentrations of vitamins and carbohydrate

Applications of Difco Soytone include:
• An alternative carbohydrate profile to yeast supplements for mammalian cell processes, such as CHO

Geneticin™ Selective Antibiotic (G418 Sulfate), Powder Gibco™

Geneticin® Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin® Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 - 200 µg/ml for bacteria, or 200 – 500 µg/ml for most mammalian cells. This product is supplied as a powder and should be made into a stock solution of 10 – 50 mg/ml in water. We offer a variety of cell culture antibiotics for your convenience.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Dual site cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

Accumax - Cell Aggregate Dissociation Medium Invitrogen™

Accumax performs exceptionally well in dissociating cell clumps for cell counting, viral transfection assays, cell sorting, and flow cytometry as well as bioreactor scale-up. Accumax has been shown effective in dissociating cell clumps in a variety of cell lines including hybridomas, CHO, BHK, 293, COS and Sf9 cells.

Notes:

1. The use of Accumax™ may effect the integrity of certain antigenic epitopes resulting in diminished antibody staining. This should be determined experimentally for individual antibody clones.

2. Thaw Accumax at room temperature or 4C.

Reported Application
Cell Culture

Defined Trypsin Inhibitor Gibco™

A sterile, phosphate-buffered saline solution (1X) containing purified soybean trypsin inhibitor. Animal origin-free.

Penicillin-Streptomycin (10,000 U/mL) Gibco™

This solution contains 10,000 units/mL of penicillin and 10,000 µg/mL of streptomycin. The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacterial cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus. It acts by binding to the 30S subunit of the bacterial ribosome, leading to inhibition of protein synthesis and death in susceptible bacteria.

We offer a wide range of antibiotics and antimycotics in both powder and liquid formats. Learn more about the following types of products:

Cell Culture Antibiotics
Selection Antibiotics (including recommended working concentrations)

Learn more about the use of antibiotics and antimycotics in cell culture and review guidelines for decontaminating cultures.

Transferrin From Human Serum, Texas Red™ Conjugate Invitrogen™

Transferrin is a monomeric serum glycoprotein (~80,000 daltons) that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe3+ atoms via receptor-mediated endocytosis—our labeled LDL complexes are useful tools for studying this phenomenon. Once iron-carrying transferrin proteins are inside endosomes, the acidic environment favors dissociation of iron from the transferrin–receptor complex. Following the release of iron, the apotransferrin is recycled to the plasma membrane, where it is released from its receptor to scavenge more iron. Fluorescent transferrin conjugates can therefore be used with fluorescent LDL to distinguish the lysosomally directed and recycling endosomal pathways.

These experiments are typically performed by adding fluorescently labeled transferrin to cultured cells and analyzing them by microscopy. We offer a biotinylated transferrin conjugate and more than 10 fluorescent versions.

Transferrin Specifications:

Label (Ex/Em): Texas Red® (595/615)

Amount: 15 mg solid (contains 5 mg of transferrin conjugate)

Key Applications of Labeled Transferrin
Some of the many applications for labeled transferrin include:
• Image transferrin receptor dynamics using FRET
• Observe receptor trafficking in live cells by confocal laser-scanning microscopy
• Investigate events occurring during endosomal acidification
• Measure transferrin receptor binding affinity in mammals and parasites

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Attachment Factor Protein (1X) Gibco™

A sterile solution (1X) containing gelatin as an attachment factor. When used to coat culture surfaces, AF enhances the growth of microvascular endothelial cells.
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