Antibiotics and Supplements

Antibiotics and supplements that enhance cell differentiation, cultivation, proliferation, and kill or slow the growth of cell culture contamination.
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Trypsin (1:250), powder Gibco™

Gibco® Trypsin 1:250 powder is an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength it is widely used for cell dissociation, during routine cell culture passaging, and primary tissue dissociation.

Polypeptone™ Peptone Gibco™

Polypeptone Peptone is a mixture of peptones made up of equal parts of pancreatic digest of casein and peptic digest of animal tissue. Polypeptone Peptone includes the high content of amino acids and small polypeptides characteristic of pancreatic digest of casein and the larger polypeptides...

Sodium Bicarbonate 7.5% solution Gibco™

Sodium Bicarbonate is buffer commonly used for maintaining the pH of cell culture medium in the presence of 4–10% carbon dioxide. In addition to buffering, sodium bicarbonate provides some nutritional benefit, while rarely displaying any cell toxicity.

Trypsin-EDTA (0.05%), phenol red Gibco™

Gibco® Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation.

L-Glutamine (200 mM) Gibco™

L-Glutamine is an amino acid that is required for cell culture. L-Glutamine participates in the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, and other amino acids, as well as in protein synthesis and glucose production.

Difco™ Beef Extract Gibco™

Difco Beef Extract is an animal origin peptone derived from an infusion of beef. This nutritional supplement provides a mixture of peptides, individual amino acids, nucleotides, organic acids, minerals, and vitamins.

Bacto™ Yeast Extract Gibco™

Bacto Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells. They are derived from primary grown baker’s yeast. These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial...

Starter Pak No. 3 Gibco™

Starter Pak No. 3 provides a variety of yeast and soy products, ideal for processes where an animal origin-free medium is preferred. It provides conveniently packaged, 100-g, non-GMP samples of these peptones that are best suited for human and animal vaccine production and contains: • Bacto Yeast...

2-Mercaptoethanol Gibco™

Gibco™ 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture media to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, so most protocols require daily supplementation.

Bacto™ Yeast Extract, technical Gibco™

Bacto Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells. They are derived from primary grown baker’s yeast. These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial...

Tryptic Soy Broth, without dextrose Gibco™

Tryptic Soy Broth without Dextrose is an enzymatic digest of casein and soybean meal. It is a low carbohydrate basal medium used for cultivating fastidious and nonfastidious microorganisms. Dextrose is omitted from the formula to permit use of the medium in fermentation studies where carbohydrates...

Trypsin-EDTA (0.25%), phenol red Gibco™

This liquid formulation of trypsin contains EDTA and phenol red. Gibco Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary...

Bacto™ TC Yeastolate Gibco™

Bacto TC Yeastolate is an animal origin-free hydrolysate from the water-soluble portion of autolyzed yeast. This supplement contains a mixture of peptides, amino acids, carbohydrates (simple and complex), nucleosides, and vitamins.

Bacto™ Casamino Acids Gibco™

Bacto Casamino Acids is an animal origin (AO) acid hydrolysis of casein. This supplement contains low salt and iron content making it an excellent supplement for media formulations where nitrogen requirements are minimal.

Bacto™ Soytone Gibco™

Bacto Soytone is an enzymatic digest of soy that uses an animal origin (AO) (porcine) enzyme for digestion. The naturally high concentrations of vitamins and carbohydrates found in soy make Bacto Soytone a nutritious and excellent carbohydrate source for those not concerned about the processing step...
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