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Blasticidin S HCl (10 mg/mL) Gibco™

Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in in bacteria and eukaryotes, while also active against fungi, nematodes, and tumor cells. Blasticidin S acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used as a selection agent in both mammalian cells and bacterial cells. The recommended working concentration ranges from 1 to 30 µg/mL depending on the cell line and 25–100 µg/mL for bacterial selection. Cell death occurs rapidly, and blasticidin-resistant stable mammalian cell lines can be generated in less than one week.

Resistance to blasticidin S is conferred by BSR and BSD, isolated from Bacillus cereus K55-S and Aspergillus terreus respectively. The BSR resistance gene encodes blasticidin S deaminase, which catalyzes the conversion of blasticidin S to deaminohydroxyblasticidin S. Deaminohydroxyblasticidin S is a biologically inactive derivative of blasticidin S and does not interact with or inhibit prokaryotic or eukaryotic ribosomes. The BSD resistance gene also encodes a blasticidin S deaminase, which catalyzes a similar reaction to the BSR deaminase. For bacterial selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0 to maintain the activity of basticidin S. A kill curve is recommended in order to determine the minimum effective blasticidin S concentration to kill non-resistant cells.

Applications
View detailed protocols on blasticidin selection in mammalian cells, E. coli, and yeast.

Tetracycline Hydrochloride Gibco™

Tetracycline is a yellow, odorless, crystalline powder. It is commonly used as an agent to activate gene expression from an inducible expression plasmid in engineered cells expressing the tetracycline repressor protein. Tetracycline Hydrochloride is the hydrochloride salt of tetracycline.

CAS Number: 64-75-5
Formula: C22H24N2O2HCl (molecular weight: 480.91)
Solubility: Soluble in water, DMSO, methanol, and ethanol. For cell culture applications, we recommend reconstituting in water.

Tetracycline Hydrochloride is supplied in a glass vial containing 500 mg with 96% purity.

Neomycin Sulfate Gibco™

Gibco® Neomycin is produced by Streptomyces fradiae. Polymyxin B Sulfate acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Gibco® Neomycin Sulfate is used for the prevention of bacterial contamination of cell cultures. This antibiotic is highly active against gram-negative bacteria. The recommended working concentration is 50 µg/ml. We offer a variety of antibiotics and antimycotics for cell culture applications.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Ampicillin, sodium salt, irradiated Gibco™

Ampicillin is an antibiotic in the broad-spectrum penicillin group. Ampicillin differs from penicillin only by the presence of an amino group, which facilitates penetration through the outer membrane of some gram-negative bacteria. Ampicillin acts by interfering directly with the turnover of the bacterial cell wall and indirectly by triggering the release of enzymes that further alter the cell wall.

Gibco Ampicillin is used as a selective antibiotic generally at a concentration of 20–125 µg/mL. Ampicillin selection is based on hydrolysis and inactivation of its beta-lactam ring by beta-lactamase expressed by the bla gene. Sometimes, beta-lactamase produced by the bacteria is secreted into the medium resulting in inactivation of ampicillin in the culture medium. Ampicillin degradation on agar plates can lead to the formation of satellite colonies. Growth of these colonies can be halted by adding fresh ampicillin. To avoid beta-lactamase buildup, special care must be taken while handling cultures and determining the correct dose of antibiotic.

Ampicillin antibiotic in powder form is an economical choice that can be used in a wide range of gram-positive and gram-negative infections. This product is provided as a powder and should be made into a stock solution at a concentration of 10 mg/mL in water.

cGMP manufacturing
Gibco Ampicillin is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Hygromycin B (50 mg/mL) Gibco™

Hygromycin B is a water-soluble antibiotic purified from the bacterium Streptomyces hydroscopicus. Hygromycin B acts by binding to the 80S subunit of the bacterial ribosome and inhibiting protein synthesis. Gibco® Hygromycin B is used as a bacterial selection antibiotic in the concentration range of 200–500 µg/mL. This product is supplied as a 50 mg/mL solution.

Resistance to Hygromycin B is conferred by the E. coli hygromycin resistance gene (hyg or hph). Hygromycin B has a different mode of action than Geneticin®, Blasticidin S, or Zeocin™ selection reagents, making it an excellent choice for dual-selection experiments (see selection antibiotics).

Other Choices and More Information
We offer a wide range of antibiotics and antimycotics in both powder and liquid formats.

See the complete list, or find products for:
Contamination control
Eukaryotic and bacterial selection

See recommendations for working concentrations for selection antibiotics.

Learn more about the use of antibiotics and antimycotics in cell culture, and review guidelines for decontaminating cultures.

Puromycin Dihydrochloride Gibco™

Puromycin Dihydrochloride is an aminonucleoside antibiotic produced by Streptomyces alboniger. Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of the pac gene.

Puromycin is widely used in cell biology as a selection antibiotic agent in mammalian cell culture systems. The recommended working concentration ranges from 0.2–5.0 µg/mL, although it can be toxic to eukaryotic cells at concentrations as low as 1 µg/mL. Gibco® Puromycin Dihydrochloride is supplied at 10 mg/mL in 20 mM HEPES buffer (pH 6.2–6.8) in 10 vials each containing 1 mL.

Other Choices and More Information
We offer a wide range of antibiotics and antimycotics in both powder and liquid formats.

See the complete list, or find products for:
Contamination control
Eukaryotic and bacterial selection

See recommendations for working concentrations for selection antibiotics.

Learn more about the use of antibiotics and antimycotics in cell culture, and review guidelines for decontaminating cultures.

(+)-1-Deoxynojirimycin Alfa Aesar™

(+)-1-Deoxynojirimycin

Gentamicin (50 mg/mL) Gibco™

Gentamicin sulfate is a water-soluble antibiotic originally purified from the fungus Micromonospora purpurea. Gentamicin acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. Gibco® Gentamicin is effective against a wide variety of gram-positive and gram-negative bacteria, and is used to prevent the contamination of cell cultures by bacteria. The recommended working concentration ranges from 0.5 to 50 µg /mL.

We offer a wide range of antibiotics and antimycotics in both powder and liquid formats.

Learn more about the use of antibiotics and antimycotics in cell culture and review guidelines for decontaminating cultures.

Dual-Site cGMP Manufacturing
Gibco® Gentamicin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® Gentamicin product made in our Scotland facility (15750-037). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Amphotericin B Gibco™

Amphotericin B is the generic version of Fungizone. 'Fungizone' is a trademark of E.R. Squibb & Sons, LLC. Amphotericin B is an antifungal, produced by Streptomyces nodosus. It prevents the growth of fungi by causing an increase in fungal plasma membrane permeability. It actively binds to sterols and leads to the formation of pores. It is used to prevent the contamination of cell cultures by yeast and multicellular fungi. Gibco Amphotericin B contains 250 µg of amphotericin B and 205 µg of sodium deoxycholate per mL of distilled water. The recommended working concentration ranges from 0.25 to 2.50 µg/mL.

Dual-site cGMP manufacturing
For supply chain continuity, we manufacture Gibco Amphotericin B at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to the ISO 13485 standard, and are registered with the FDA as medical device manufacturers.

Penicillin-Streptomycin-Neomycin (PSN) Antibiotic Mixture Gibco™

The antibiotics Penicillin, Streptomycin, and Neomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungus Penicillium and acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus and Neomycin from Streptomyces fradiae. Streptomycin and Neomycin act by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria. We offer a variety of antibiotics and antimycotics for cell culture applications.

This solution contains 5 mg of penicillin, 5 mg of streptomycin, and 10 mg of neomycin per ml in 0.85% saline.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Geneticin™ Selective Antibiotic (G418 Sulfate), Powder Gibco™

Geneticin® Selective Antibiotic (G418 Sulfate) is produced by the bacterium Micromonospora rhodorangea and acts by binding the ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells. Resistance to Geneticin® Selective Antibiotic (G418 Sulfate) is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes. Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) is used as a selective antibiotic in the concentration range of 100 - 200 µg/ml for bacteria, or 200 – 500 µg/ml for most mammalian cells. This product is supplied as a powder and should be made into a stock solution of 10 – 50 mg/ml in water. We offer a variety of cell culture antibiotics for your convenience.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Dual site cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture Gibco® Geneticin® Selective Antibiotic (G418 Sulfate) at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

Penicillin-Streptomycin (10,000 U/mL) Gibco™

This solution contains 10,000 units/mL of penicillin and 10,000 µg/mL of streptomycin. The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. Penicillin was originally purified from the fungi Penicillium and acts by interfering directly with the turnover of the bacterial cell wall and indirectly by triggering the release of enzymes that further alter the cell wall. Streptomycin was originally purified from Streptomyces griseus. It acts by binding to the 30S subunit of the bacterial ribosome, leading to inhibition of protein synthesis and death in susceptible bacteria.

We offer a wide range of antibiotics and antimycotics in both powder and liquid formats. Learn more about the following types of products:

Cell Culture Antibiotics
Selection Antibiotics (including recommended working concentrations)

Learn more about the use of antibiotics and antimycotics in cell culture and review guidelines for decontaminating cultures.

Blasticidin S HCl, powder Gibco™

Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in bacteria and eukaryotes, while also active in fungi, nematodes, and tumor cells. Blasticidin S acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used as a selection agent in both mammalian and bacterial cells. The recommended working concentration ranges from 1 to 30 µg/mL depending on the cell line and 25–100 µg/mL for bacterial selection. Cell death occurs rapidly, and blasticidin-resistant stable mammalian cell lines can be generated in less than one week.

Resistance to blasticidin S is conferred by BSR and BSD, isolated from Bacillus cereus K55-S and Aspergillus terreus respectively. The BSR resistance gene encodes blasticidin S deaminase, which catalyzes the conversion of blasticidin S to deaminohydroxyblasticidin S. Deaminohydroxyblasticidin S is a biologically inactive derivative of blasticidin S and does not interact with or inhibit prokaryotic or eukaryotic ribosomes. The BSD resistance gene also encodes a blasticidin S deaminase, which catalyzes a similar reaction to the BSR deaminase.

For bacterial selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0 to maintain the activity of basticidin S. A kill curve is recommended in order to determine the minimum effective blasticidin S concentration to kill non-resistant cells.

Applications
View detailed protocols on blasticidin selection in mammalian cells, E. coli, and yeast.

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