Shop All Dissociation Reagents

Genomics and Proteomics


Protein Family


Cholesterol Lipid Concentrate (250X) Gibco™

250X Cholesterol Lipid Concentrate is a protein-free, chemically-defined supplement used for growing NSO and other lipid dependent cell lines to high densities while supporting growth and protein expression without complicating downstream purification. The supplement can be added to 1X media such as CD Hybridoma and filtered with minimal loss of lipids. Custom formulations and packaging configurations as well as supplements used in feeding strategies are available through our Custom Products Services group.

KaryoMAX™ Colcemid™ Solution in PBS Gibco™

Gibco® KaryoMAX® Colcemid™ Solution in PBS is a 10 µg/ml N-desacetyl-N-methylocolchicine (Colcemid™) solution made in Phosphate Buffered Saline (PBS). It is used for arresting cells in metaphase so the chromosomes can be separated for cytogenetic studies and in vitro diagnostic procedures. Colcemid™ prevents spindle formation during mitosis, causing metaphase arrest. The mechanism of action is similar to that of colchicine, but with lower mammalian toxicity. The complete formulation is available.

Product Use
For in vitro diagnostic use.

cGMP Manufacturing and Quality System
Gibco® KaryoMAX® Colcemid™ Solution in PBS is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Expi293F™ Cells Gibco™

Expi293F human cells are derived from the 293 cell line, and are a core component of the Expi293 Expression System. They are maintained in suspension culture and will grow to high density in Expi293 Expression Medium. Expi293F cells are highly transfectable and generate superior protein yields compared to standard 293 cell lines in transient protein expression.

Expi293F Cells attributes include:
Suspension culture cells pre-adapted to Expi293 Expression Medium
Demonstrated higher protein expression levels than other 293 cells adapted to Expi293 Expression Medium
Exhibit more rapid cell growth and higher culture viabilities than standard suspension-adapted 293 cells
Consistent transient expression performance of Expi293F cells for over 40 passages beyond thaw
• Also available from a cGMP bank (Cat. No. 100044202)

Frozen cells are supplied in a vial containing 1 ml of cells at 1 x 107 viable cells/ml in Expi293 Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293 Expression Medium. The six-vial multipack eliminates the need to expand and rebank the cells in the lab.

Cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of <1 mL in multiwell plates to >10 L in bioreactors. Protein harvest can be performed at 2 to 7 days post-transfection, depending on the protein expressed. For expression of recombinant antibodies, harvest is typically at 5 to 7 days post-transfection. There is no need for culture medium supplementation or batch feeding during expression.

Trypan Blue Solution, 0.4% Gibco™

Trypan Blue Solution, 0.4%, is routinely used as a cell stain to assess cell viability using the dye exclusion test. This test is often performed while counting cells with the hemocytometer during routine subculturing, but can be performed any time cell viability needs to be determined quickly and accurately. The dye exclusion test is based upon the concept that viable cells do not take up impermeable dyes (like Trypan Blue), but dead cells are permeable and take up the dye.

cGMP manufacturing and quality system
Trypan Blue Solution is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

2-Mercaptoethanol Gibco™

Gibco™ 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture media to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, so most protocols require daily supplementation. Gibco 2-Mercaptoethanol contains 2-mercaptoethanol at a concentration of 55 mM in Dulbecco's phosphate buffered saline (DPBS).

cGMP manufacturing and quality system
Gibco Reducing Agent is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

BacMam Enhancer Solution (1000X) Invitrogen™

The BacMam Enhancer helps to increase expression of BacMam reagents in mammalian cells.

To use, simply add the BacMam enhancer to cell culture media with or without serum that have already been treated with the BacMam reagent, then incubate cells and replace with fresh medium. Different cell types vary in their response to the BacMam enhancer and for best results the amount and treatment time will need to be optimized.

Transferrin From Human Serum, Fluorescein Conjugate Invitrogen™

Transferrin is a monomeric serum glycoprotein (~80,000 daltons) that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe3+ atoms via receptor-mediated endocytosis—our labeled LDL complexes are useful tools for studying this phenomenon. Once iron-carrying transferrin proteins are inside endosomes, the acidic environment favors dissociation of iron from the transferrin–receptor complex. Following the release of iron, the apotransferrin is recycled to the plasma membrane, where it is released from its receptor to scavenge more iron. Fluorescent transferrin conjugates can therefore be used with fluorescent LDL to distinguish the lysosomally directed and recycling endosomal pathways.

These experiments are typically performed by adding fluorescently labeled transferrin to cultured cells and analyzing them by microscopy. We offer a biotinylated transferrin conjugate and more than 10 fluorescent versions.

Transferrin Specifications:

Label (Ex/Em): Fluorescein (495/518)

Amount: 15 mg solid (contains 5 mg of transferrin conjugate)

Key Applications of Labeled Transferrin
Some of the many applications for labeled transferrin include:
• Image transferrin receptor dynamics using FRET
• Observe receptor trafficking in live cells by confocal laser-scanning microscopy
• Investigate events occurring during endosomal acidification
• Measure transferrin receptor binding affinity in mammals and parasites

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Soybean Trypsin Inhibitor, powder Gibco™

Soybean Trypsin Inhibitor is one of several protease inhibitors found in soybeans. Trypsin is inhibited at a molar ratio of 1:1, while chymotrypsin and plasmin are inhibited to a lesser extent. Soybean trypsin inhibitor will also inhibit other serine proteases.

Gibco® Soybean Trypsin Inhibitor, powder has an activity greater than 7,000 BAEE units per mg. A 0.5 mg/mL Soybean Trypsin Inhibitor stock solution should be made by dissolving the non-sterile powder in 0.01M Borate Buffer or Dulbecco's Phosphate Buffered Saline (DPBS), without calcium and magnesium. Filter sterilize the stock solution and store for up to a week at 2 - 8°C.

Product Use
For Research Use Only. Not for use in diagnostic procedures.

Histone H1 From Calf Thymus, Alexa Fluor™ 488 Conjugate Invitrogen™

The Alexa Fluor® 488 histone H1 conjugate is labeled with the bright and photostable Alexa Fluor® 488 dye and exhibits fluorescence with excitation/emission maxima ~495/520 nm and can be visualized by fluorescence microscopy or flow cytometry.

Transferrin From Human Serum, Alexa Fluor™ 568 Conjugate Invitrogen™

Transferrin is a monomeric serum glycoprotein (~80,000 daltons) that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe3+ atoms via receptor-mediated endocytosis—our labeled LDL complexes are useful tools for studying this phenomenon. Once iron-carrying transferrin proteins are inside endosomes, the acidic environment favors dissociation of iron from the transferrin–receptor complex. Following the release of iron, the apotransferrin is recycled to the plasma membrane, where it is released from its receptor to scavenge more iron. Fluorescent transferrin conjugates can therefore be used with fluorescent LDL to distinguish the lysosomally directed and recycling endosomal pathways.

These experiments are typically performed by adding fluorescently labeled transferrin to cultured cells and analyzing them by microscopy. We offer a biotinylated transferrin conjugate and more than 10 fluorescent versions.

Transferrin Specifications:

Label (Ex/Em): Alexa Fluor® 568 (578/603)

Amount: 15 mg solid (contains 5 mg of transferrin conjugate)

Key Applications of Labeled Transferrin
Some of the many applications for labeled transferrin include:
• Image transferrin receptor dynamics using FRET
• Observe receptor trafficking in live cells by confocal laser-scanning microscopy
• Investigate events occurring during endosomal acidification
• Measure transferrin receptor binding affinity in mammals and parasites

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

HEPES (Ultra Pure) Gibco™

Gibco® HEPES (N-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10 – 25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incubator. HEPES is a good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stable, and has very low visible and UV light absorbance.

Product Use
For Research Use Only. Not for use in diagnostic procedures.

Low Density Lipoprotein from Human Plasma, DiI complex (DiI LDL) Invitrogen™

Human low-density lipoprotein (LDL) is large protein complex (~500,000 Da) that binds to a specific receptor on the surface of vertebrate cells and delivers cholesterol via receptor-mediated endocytosis—our labeled LDL complexes are useful tools for studying this phenomenon. These experiments are typically performed by adding fluorescently labeled LDL to cultured cells and analyzing them by microscopy or flow cytometry. Alternatively the fluorescently labeled LDL can be injected into test animals, and the distribution of the label can be analyzed after the specified time period. We offer an unlabeled LDL and two classes of labeled LDLs: those containing an unmodified apoprotein (used to study normal cholesterol delivery and internalization) and those with an acetylated (Ac) apoprotein (used to study cell types that express receptors specific for this acetylated version (i.e., endothelial and microglial cells)).

LDL Specifications:

Label (Ex/Em): Dil (554/571)
Acetylated: No
Amount: 200 µL (1.0 mg/mL)

Fresh LDL Produced Continually
We prepare our LDL and AcLDL products from fresh human plasma approximately every two months. The nonacetylated LDL products are shipped within two weeks of their preparation. All acetylated LDL products are available on a continuous basis.

Nonacetylated vs. Acetylated LDL
LDL containing an unmodified apoprotein is used to study normal cholesterol delivery and internalization. If the lysine residues of LDL’s apoprotein have been acetylated, the LDL complex no longer binds to the LDL receptor, but rather is taken up by endothelial and microglial cells that possess “scavenger” receptors specific for that modified form.

Key Applications for Labeled LDL
Some of the many applications for labeled LDL complexes include:
• Counting cell-surface LDL receptors, and analyzing their motion and clustering following internalization
• Quantitating LDL receptor activity in fibroblasts (replacing the radiolabeled LDL assay)
• Investigating LDL expression and identifying LDL receptor deficiencies in various cell lines

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Transferrin From Human Serum, Tetramethylrhodamine Conjugate Invitrogen™

Transferrin is a monomeric serum glycoprotein (~80,000 daltons) that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe3+ atoms via receptor-mediated endocytosis—our labeled LDL complexes are useful tools for studying this phenomenon. Once iron-carrying transferrin proteins are inside endosomes, the acidic environment favors dissociation of iron from the transferrin–receptor complex. Following the release of iron, the apotransferrin is recycled to the plasma membrane, where it is released from its receptor to scavenge more iron. Fluorescent transferrin conjugates can therefore be used with fluorescent LDL to distinguish the lysosomally directed and recycling endosomal pathways.

These experiments are typically performed by adding fluorescently labeled transferrin to cultured cells and analyzing them by microscopy. We offer a biotinylated transferrin conjugate and more than 10 fluorescent versions.

Transferrin Specifications:

Label (Ex/Em): Tetramethylrhodamine (555/580)

Amount: 15 mg solid (contains 5 mg of transferrin conjugate)

Key Applications of Labeled Transferrin
Some of the many applications for labeled transferrin include:
• Image transferrin receptor dynamics using FRET
• Observe receptor trafficking in live cells by confocal laser-scanning microscopy
• Investigate events occurring during endosomal acidification
• Measure transferrin receptor binding affinity in mammals and parasites

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Trypsin-EDTA (0.25%), phenol red Gibco™

This liquid formulation of trypsin contains EDTA and phenol red. Gibco Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco trypsin solutions and recommended dissociation conditions.

This trypsin solution is modified as follows:
With
EDTA
Phenol Red

The complete formulation is available.

We offer a variety of trypsin formulations, including animal origin-free TrypLE reagents.

Quality testing
Gibco Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, prior to manufacturing, the raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.

Documented traceability
We can provide detailed documentation to meet your regulatory needs. Gibco Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.

cGMP manufacturing and quality system
Gibco Trypsin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.



Transferrin From Human Serum, Alexa Fluor™ 647 Conjugate Invitrogen™

Transferrin is a monomeric serum glycoprotein (~80,000 daltons) that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe3+ atoms via receptor-mediated endocytosis—our labeled LDL complexes are useful tools for studying this phenomenon. Once iron-carrying transferrin proteins are inside endosomes, the acidic environment favors dissociation of iron from the transferrin–receptor complex. Following the release of iron, the apotransferrin is recycled to the plasma membrane, where it is released from its receptor to scavenge more iron. Fluorescent transferrin conjugates can therefore be used with fluorescent LDL to distinguish the lysosomally directed and recycling endosomal pathways.

These experiments are typically performed by adding fluorescently labeled transferrin to cultured cells and analyzing them by microscopy. We offer a biotinylated transferrin conjugate and more than 10 fluorescent versions.

Transferrin Specifications:

Label (Ex/Em): Alexa Fluor® 647 (650/665)

Amount: 15 mg solid (contains 5 mg of transferrin conjugate)

Key Applications of Labeled Transferrin
Some of the many applications for labeled transferrin include:
• Image transferrin receptor dynamics using FRET
• Observe receptor trafficking in live cells by confocal laser-scanning microscopy
• Investigate events occurring during endosomal acidification
• Measure transferrin receptor binding affinity in mammals and parasites

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Results per page
    spinner