Additional Selective and Differential Culture Media

EpiLife™ CF Kit Gibco™

A sterile, liquid medium prepared without calcium chloride for the long-term, serum-free culture of human epidermal keratinocytes and human corneal epithelial cells. This basal medium requires the addition of calcium plus an appropriate supplement prior to use.

StemFlex™ Medium Gibco™

Gibco™ StemFlex™ Medium supports the robust expansion of feeder-free pluripotent stem cells (PSCs) and is optimized to support novel applications, including single-cell passaging, gene editing, and reprogramming.

Human Vascular Smooth Muscle Cell Basal Medium (formerly ''Medium 231'') Gibco™

Human Vascular Smooth Muscle Cell Basal Medium (formerly “Medium 231”) is a sterile, liquid culture medium intended for use as one component in a complete culture environment for the growth of normal human vascular smooth muscle cells.

Human Fibroblast Expansion Basal Medium (formerly Medium 106) Gibco™

Human Fibroblast Expansion Basal Medium (formerly “Medium 106”) is a sterile, liquid tissue culture medium intended for use as one component in a complete culture environment for the growth of normal human dermal fibroblasts.

MEM, glutamine, HEPES, powder Gibco™

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes.

CTS™ OpTmizer™ T Cell Expansion SFM, bottle format Gibco™

GIBCO OpTmizer™ CTS™ T-Cell Expansion SFM has been developed for the growth and expansion of human T lymphocytes. OpTmizer™ CTS™ T-Cell Expansion medium is a complete serum-free, xeno-free 1X medium consisting of OpTmizer™ T-Cell Expansion Basal Medium (1 L bottle) and OpTmizer™ T-Cell Expansion...

EBSS, calcium, magnesium, phenol red Gibco™

Earle's Balanced Salt Solution (EBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.

Leibovitz's L-15 Medium, GlutaMAX™ Supplement Gibco™

Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate.

LHC-8 Medium (1X) Gibco™

LHC serum-free media are the most widely used products for culture of bronchial epithelial cells. For maximum flexibility and convenience, we offer these products with or without supplementation. LHC Basal Medium (1X), liquid Applications: Formulated for the growth of bronchial epithelial cells with...

CHO-S-SFM II (without hypoxanthine or thymidine) Gibco™

CHO-S-SFM II, is a complete, serum-free, low-protein (less than 100 µg/ml) medium optimized for the growth and maintenance of Chinese hamster ovary (CHO) cells in suspension culture for production of recombinant proteins.

Chromosome Medium 1A Gibco™

Gibco™ Chromosome Medium 1A is a complete medium for the culture of lymphocytes isolated from bone marrow for cytogenetic studies. Lymphocytes from peripheral blood can also be cultured in Gibco™ Chromosome Medium 1A, with the addition of phytohemagglutinin (PHA).

McCoy's 5A (Modified) Medium, GlutaMAX™ Supplement Gibco™

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat...

Keratinocyte SFM (1X), without calcium chloride Gibco™

Keratinocyte SFM (serum-free medium) is optimized for the growth and maintenance of human keratinocytes and other types of epithelial cells without the need for a feeder layer of cells. The complete medium may be used to cultivate human epidermal keratinocytes for studies involving dermal...

Medium 154CF, Kit Gibco™

A sterile, liquid medium for the culture of human epidermal keratinocytes. Medium 154CF is Medium 154 prepared without calcium chloride. Calcium chloride is provided as a separate component with each bottle of medium.

EpiLife™ CF/PRF Kit Gibco™

A sterile, liquid medium for the long-term, serum-free culture of human epidermal keratinocytes and human corneal epithelial cells. EpiLife® CF⁄PRF is prepared without calcium chloride and phenol red. Because this product is phenol red free, this product is ideal for oncology studies and when...

Medium 154CF/PRF, Kit Gibco™

A sterile, liquid medium for the culture of human epidermal keratinocytes. Medium 154CF⁄PRF is Medium 154 prepared without calcium chloride or phenol red. Calcium chloride is provided as a separate component with each bottle of medium.

Medium 154 Gibco™

A sterile, liquid medium prepared with 200 µM calcium chloride for the culture of human epidermal keratinocytes. This basal medium requires the addition of HKGS (S-001-5) or HKGS Kit (S-001-K) prior to use.

Medium 254 Gibco™

Medium 254 is a sterile, liquid tissue culture medium prepared with 200 µM calcium chloride for the culture of normal human epidermal melanocytes. This medium is intended for use as one component in a complete culture environment.

TAP Growth Media, optimized for Chlamydomonas culture Gibco™

Gibco™ TAP Media is optimized for the growth and maintenance of Chlamydomonas reinhardtii. The formulation comes ready-to-use at 1X to let you avoid laborious media preparation steps. Each Gibco™ TAP Media comes in the award winning Gibco™ bottle designed for easier use in the biosafety cabinet,...

CD BHK-21 Production Medium Gibco™

Gibco™ CD BHK-21 Production Medium is a chemically defined, protein-free cell culture medium. It is designed to support the growth of BHK-21 cells in serum-free suspension culture. This medium is suitable for large-scale manufacturing applications.

SILAC RPMI 1640 Flex Media, no glucose, no phenol red Gibco™

SILAC RPMI 1640 Medium is RPMI 1640 basal cell culture medium without L-arginine, L-glutamine, and L-lysine for use during SILAC protein labeling with stable isotope-labeled lysine and/or arginine. RPMI 1640 Medium is widely used for supporting the growth of many different mammalian cells.

CTS™ Neurobasal™-A Medium Gibco™

CTS Neurobasal-A Medium is designed with the translational stem cell researcher in mind. It can be used to culture post-natal neurons without the need for an astrocyte feeder layer when used with B-27 supplements.

CD OptiCHO™ AGT™ Medium Gibco™

CD OptiCHO™ medium is specifically designed to offer high performance and yield with recombinant CHO cells in a chemically defined fed-batch environment. The protein-free, animal origin free formulation is designed to provide consistency and reduces the need to screen for adventitious agents.

HepExtend™ Media Kit Gibco™

The HepExtend™ Media Kit contains the new HepExtend Supplement (50X), as well as William's E Medium and Primary Hepatocyte Maintenance Supplement, providing the three components needed to optimize long term hepatocyte culture.

Diploid Growth Serum-Reduced Medium (SRM) Gibco™

The Gibco Diploid Serum-Reduced Medium (SRM) system is designed to support vaccine manufacture under serum-reduced or serum-free conditions for cell types traditionally cultured in classical media with 10% serum.

CTS™ OpTmizer™ T Cell Expansion SFM, bag format Gibco™

Gibco™ OpTmizer™ CTS™ T-Cell Expansion SFM has been developed for the growth and expansion of human T lymphocytes. OpTmizer™ CTS™ T-Cell Expansion medium is a complete serum-free and xeno-free 1X medium consisting of OpTmizer™ T-Cell Expansion Basal Medium (1 L bag) and OpTmizer™ T-Cell Expansion...

McCoy's 5A Medium (Park and Terasaki modification) Thermo Scientific™

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat...

Breast Cancer Cell Culture Starter Kit, North America Gibco™

Gibco Cancer Cell Culture Starter kits provide researchers with a convenient package of reagents for the culture and transfection of cancer cell types frequently used in cancer research. Cancer Cell Culture Starter kits are currently available for breast, lung, prostate, and liver cancer cells.

Prostate Cancer Cell Culture Starter Kit, North America Gibco™

Gibco Cancer Cell Culture Starter kits provide researchers with a convenient package of reagents for the culture and transfection of cancer cell types frequently used in cancer research. Cancer Cell Culture Starter kits are currently available for breast, lung, prostate, and liver cancer cells.

CTS™ OpTmizer™ T-Cell Expansion SFM, no phenol red, bottle format Gibco™

Gibco CTS OpTmizer T-Cell Expansion SFM supports the growth and expansion of human T lymphocytes. It is a complete serum-free, xeno-free medium consisting of CTS OpTmizer T-Cell Expansion Basal Medium and CTS OpTmizer T-Cell Expansion Supplement, which are mixed together prior to use.

CTS™ OpTmizer™ T-Cell Expansion SFM, no phenol red, bag format Gibco™

Gibco CTS OpTmizer T-Cell Expansion SFM supports the growth and expansion of human T lymphocytes. It is a complete serum-free, xeno-free medium consisting of CTS OpTmizer T-Cell Expansion Basal Medium and CTS OpTmizer T-Cell Expansion Supplement, which are mixed together prior to use.

CTS™ OpTmizer™ Pro SFM, bottle format Gibco™

Gibco CTS OpTmizer Pro SFM, no phenol red, is a novel serum-free medium (SFM) developed for the growth and expansion of human T lymphocytes (e.g., CD4+, CD8+, polyclonal, or antigen specific) used in allogeneic cell therapies.

Medium 199, Earle's Salts (10X) Gibco™

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse...

Liver Perfusion Medium (1X) Gibco™

Liver Perfusion Medium is a buffered, balanced salt solution formulated to cleanse the liver of blood, prevent clotting, and initiate loosening of cell-to-cell contact.

CMRL Medium (10X), no glutamine Gibco™

CMRL Medium was originally developed by Connaught Medical Research Laboratories for the growth of Earle's 'L' cells under serum-free conditions. Gibco™ CMRL Medium is also especially useful for cloning monkey kidney cells and for growth of many other mammalian cell lines when supplemented with horse...

AIM V™ Medium, liquid (research grade) Gibco™

Contains L-glutamine, streptomycin sulfate at 50 µg/ml, and gentamicin sulfate at 10 µg/ml.

Adenovirus Expression Medium (AEM), for PER.C6™ cells Gibco™

Adenovirus Expression Medium (AEM) is recommended for the growth of PER.C6® cells in suspension and for the production of viruses and viral vectors in these cells. It is an animal-origin free cell culture medium without components directly derived from human or animal sources.

AmnioMAX™ C-100 Complete Medium Gibco™

Gibco™ AmnioMAX™ C-100 Complete Medium is a kit used to prepare a fully-supplemented medium developed for the short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures.

CD DG44 Medium Gibco™

CD DG44 Medium is a chemically defined, protein-free medium optimized for the growth of dihydrofolate reductase deficient (DHFR-) Chinese Hamster Ovary (CHO) cells and expression of recombinant proteins in suspension culture.

AmnioMAX™-II Complete Medium Gibco™

Gibco™ AmnioMAX™ - II Complete Medium is a fully-supplemented medium developed for the short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures. Gibco™ AmnioMAX™ - II Complete Medium features: • Ready-to-use format • Quality and performance testing...

PFHM-II Protein-Free Hybridoma Medium (liquid) Gibco™

Gibco® Protein Free Hybridoma Medium II (PFHM II) is an animal origin-free, serum-free, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production.

CHO-S-SFM II, with hypoxanthine and thymidine Gibco™

CHO-S-SFM II, is a complete, serum-free, low-protein (less than 100 µml) medium optimized for the growth and maintenance of Chinese hamster ovary (CHO) cells in suspension culture for production of recombinant proteins.

Ham's F-10 Nutrient Mix, GlutaMAX™ Supplement Gibco™

Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate...

RPMI 1640 Medium, low HEPES, low bicarbonate, no glutamine Gibco™

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

Keratinocyte SFM (1X) Gibco™

Keratinocyte SFM (serum-free medium) is optimized for the growth and maintenance of human keratinocytes and other types of epithelial cells without the need for a feeder layer of cells. The complete medium may be used to cultivate human epidermal keratinocytes for studies involving dermal...

HepatoZYME-SFM Gibco™

Hepatozyme-SFM is a serum-free medium for the long-term maintenance of hepatocyte phenotypic expression, including active and inducible forms of cytochromes P450 and active phase II enzymes. Contains no phenol red.

MEM Rega-3 Gibco™

Minimum Essential Medium (MEM) REGA-3 is a modification of the commonly used MEM. The Rega-3 modification is often used to culture HEP-G2 (human liver carcinoma) cells and occasionally other human tumor cells. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications.

293 SFM II Gibco™

293 SFM II is a serum-free, low-protein (<10 µg/ml) formulation optimized for high-density suspension culture of 293 cells (1). (It is not suitable for adherent 293 cell cultures.) This product replaces 293 SFM (Cat. No. 10970-010). 293 SFM II is animal-origin free.

CD 293 AGT™ Medium Gibco™

CD 293 AGT™ is a chemically-defined, protein-free medium in a new easy-to-use granular format. CD 293 AGT™ is optimized for the growth of suspension cultures of 293 cells. Advanced Granulation Technology = AGT

William's E Medium, no glutamine Gibco™

William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. Gibco™ William's E Medium can also be used for growing hepatocyte cells of different species, such as human derived HepaRG™ cells.

Protein Expression Medium (PEM) Gibco™

• Supports the large-scale, high-density culture of transfected PER.C6™ cells in shake flasks, roller bottles, and bioreactors • Contains no components directly derived from human or animal sources and one low-level, recombinant protein, minimizing the opportunity to introduce adventitious agents •...

LHC-8 Medium (1X), without gentamicin Gibco™

LHC serum-free media are the most widely used products for culture of bronchial epithelial cells. This is a supplemented medium. For maximum flexibility and convenience, we also offer LHC basal medium.

SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red Gibco™

SILAC Advanced DMEM/F-12 Flex is Advanced DMEM/F-12 without L-arginine, L-glutamine, and L-lysine for use during SILAC protein labeling with stable isotopic labeled lysine and/or arginine. Advanced DMEM/F-12 is a widely used basal medium for supporting the growth of many different mammalian cells,...