Additional Selective and Differential Culture Media

NCTC-135 Medium Gibco™

NCTC-135 medium is a chemically defined medium that was originally developed at the National Cancer Institute (Bethesda, MD) and formulated for the culture of mouse Strain L sublines, NCTC clone 929 (L929 cells) in a serum-free environment.

Medium NCTC-109 (1X) Gibco™

NCTC-109 medium is a chemically defined medium that was originally developed by the National Cancer Institute and formulated for the culture of mouse Strain L sublines, NCTC clone 929 (L929 cells) in a serum-free environment.

VP-SFM (1X) Gibco™

VP-SFM is a serum-free, ultra-low protein (5 µg/ml) medium containing no proteins, peptides, or other components of animal or human origin. It is designed for the growth of VERO cells for virus production (1).

Macrophage-SFM (1X) Gibco™

Macrophage-SFM is a serum-free medium designed for the culture of human peripheral blood monocytes and macrophages (1). Contains L-glutamine, but no antibiotics.

CD 293 Medium (1X) Gibco™

CD 293 Medium is a chemically-defined, protein-free medium optimized for the growth of suspension cultures of 293 cells. CD 293 Medium contains no proteins or peptides of animal, plant, or synthetic origin. There are also no undefined lysates or hydrolysates in the formulation.

Defined Keratinocyte SFM (1X) Gibco™

Defined Keratinocyte SFM is a serum-free medium optimized for the isolation and expansion of human keratinocytes without the need for bovine pituitary extract (BPE) supplementation or the use of fibroblast feeder layers.

EpiSerf Medium Thermo Scientific™

Gibco™ EpiSerf Medium is a serum-free medium for the culture of a wide variety of adherent cells, including primary epithelial cells, MDBK, MDCK, VERO, and CHO. The growth and morphology is comparable to the same cells grown in a classic medium supplemented with Fetal Bovine Serum (FBS).

MEM, Hanks' Balanced Salts, powder Gibco™

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes.

FreeStyle™ F17 Expression Medium Gibco™

Gibco FreeStyle F17 Expression Medium is a chemically defined, protein-free, serum-free medium developed to support the growth and transfection of HEK293 cells in suspension. The addition of 4–8 mM L-glutamine or GlutaMAX supplement is required before use.

Fischer's Medium Gibco™

Fischer's medium was originally developed by Glen Fischer at the National Cancer Institute (Bethesda, MD) and formulated for the culture of mouse leukemic cells. Fischer's Medium is primarily used for the culture of hematopeitic cells, such as lymphoblasts and lymphocytes.

Dynamis™ Medium Gibco™

Gibco Dynamis liquid medium is specifically designed to offer the highest batch and fed-batch culture performance and yield with recombinant CHO cells in a chemically defined environment. Gibco Dynamis medium has also been shown to support robust growth of HEK293 cells for adenovirus production.

Human Large Vessel Endothelial Cell Basal Medium (formerly Medium 200) Gibco™

Human Large Vessel Endothelial Cell Basal Medium (formerly “Medium 200”) is a sterile, liquid tissue culture medium intended for use as one component in a complete culture environment for normal human large vessel endothelial cells.

Human Melanocyte Basal Medium Kit, calcium free (formerly Medium 254CF Kit) Gibco™

The Gibco Human Melanocyte Basal Medium Kit, Calcium Free (formerly Medium 254CF Kit), enables the culture of human epidermal melanocytes. The kit contains two components: • Human Melanocyte Basal Medium, Calcium Free (formerly Medium 254CF)—a calcium-free version of Human Melanocyte Basal Medium...

Human Mammary Epithelial Cell Basal Medium (formerly ''Medium 171'') Gibco™

Human Mammary Epithelial Cell Basal Medium (formerly ''Medium 171'') is a sterile, liquid tissue culture medium intended for use in a complete culture environment for the growth of normal human mammary epithelial cells.

RPMI 1640 Medium, HEPES, no glutamine Gibco™

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

Liver Digest Medium Gibco™

Liver Digest Medium is a collagenase-dispase medium for dissociation of viable liver cells.

Expi293™ Expression Medium Gibco™

Gibco Expi293 Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F cells in the Expi293...

Diploid Production Serum-Free Medium (SFM) Gibco™

The Gibco Diploid Serum-Reduced Medium (SRM) system is designed to support vaccine manufacture under serum-reduced or serum-free conditions for cell types traditionally cultured in classical media with 10% serum.

CTS™ OpTmizer™ Pro SFM, bag format Gibco™

Gibco CTS OpTmizer Pro SFM, no phenol red, is a novel serum-free medium (SFM) developed for the growth and expansion of human T lymphocytes (e.g., CD4+, CD8+, polyclonal, or antigen specific) used in allogeneic cell therapies.

Lung Cancer Cell Culture Starter Kit, North America Gibco™

Gibco Cancer Cell Culture Starter kits provide researchers with a convenient package of reagents for the culture and transfection of cancer cell types frequently used in cancer research. Cancer Cell Culture Starter kits are currently available for breast, lung, prostate, and liver cancer cells.

SILAC DMEM Flex Media, no glucose, no phenol red Gibco™

SILAC DMEM Flex is Dulbecco’s Modified Eagle Medium (DMEM) without L-glutamine, L-lysine, and L-arginine for use during SILAC protein labeling with stable isotopic labeled lysine and/or arginine. DMEM is a widely used basal medium for supporting the growth of many different mammalian cells.

CTS™ Opti-MEM™ I Medium Gibco™

Gibco CTS Opti-MEM I Medium is an improved minimal essential medium (MEM) that is recommended for use with the CTS LV-MAX Transfection Kit as a complexation buffer. This medium contains insulin, transferrin, hypoxanthine, thymidine, and trace elements.

Liver Cancer Cell Culture Starter Kit, North America Gibco™

Gibco Cancer Cell Culture Starter kits provide researchers with a convenient package of reagents for the culture and transfection of cancer cell types frequently used in cancer research. Cancer Cell Culture Starter kits are currently available for breast, lung, prostate, and liver cancer cells.