Shop All Additional Selective and Differential Culture Media

RPMI 1640 Medium, low HEPES, low bicarbonate, no glutamine Gibco™

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI 1640 is modified as follows:
WithWithout
• Phenol Red• L-glutamine
• HEPES

The complete formulation is available.

Gibco® RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® RPMI 1640 is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

AmnioMAX™-II Complete Medium Gibco™

Gibco® AmnioMAX™ - II Complete Medium is a fully-supplemented medium developed for the short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures.

Gibco® AmnioMAX™ - II Complete Medium features:

• Ready-to-use format
• Quality and performance testing
• Unique, optimized formulation

Ready-to-use format
Gibco® AmnioMAX™ - II Complete Medium is a frozen, 1X medium, ready to use upon thawing. No supplementation is required. Thawed medium can be stored at 2–8°C for up to ten days.

Quality and performance testing
Every lot of Gibco® AmnioMAX™ - II Complete Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Pooled primary human amniotic fluid samples are cultured for six days in Gibco® AmnioMAX™ - II Complete Medium before measuring the total number of colonies and total number of mitotic colonies. In addition, each lot is tested for sterility, pH and osmolality.

Unique, optimized formulation
Gibco® AmnioMAX™ - II Complete Medium contains Fetal Bovine Serum (FBS), gentamicin, and L-glutamine to maximize cell attachment and growth. This optimized medium also has an enhanced buffering system that provides greater pH stability during culture manipulations.

Product Use
For in vitro diagnostic use.

cGMP Manufacturing and Quality System
Gibco® AmnioMAX™ - II Complete Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Medium 106 Gibco™

A sterile, liquid medium for the culture of human dermal fibroblasts. This basal medium requires the addition of LSGS (S-003-10) or LSGS Kit (S-003-K) prior to use.

VP-SFM AGT™ Medium Gibco™

VP-SFM AGT™ is a serum free, ultra low protein medium (5 µg/ml) in a new easy-to-use granular format. VP-SFM AGT™ is designed for the growth of VERO cells for virus production. It is also suitable for the growth of COS-7, MDCK, BHK-21 and Hep2 cells and for recombinant protein production.

CD BHK-21 Production Medium Gibco™

Gibco® CD BHK-21 Production Medium is a chemically defined, protein-free cell culture medium. It is designed to support the growth of BHK-21 cells in serum-free suspension culture. This medium is suitable for large-scale manufacturing applications.

CD BHK-21 Production Medium is manufactured as follows:
WithWithout
• L-glutamine• Phenol Red
• Dry powder formatProtein hydrolysates

Gibco® CD BHK-21 Production Medium is the first cell culture medium supporting complete serum-free and protein-free processes for BHK-21 cells. The dry powder format is easy to reconstitute, since it has no hydrolysates in the formulation. Adapting cells from serum-containing media is a rapid and simple procedure. BHK-21 cells can achieve high cell densities and maintain high cell viability in this medium.

Gibco® CD BHK-21 Production Medium is suitable for large-scale suspension culture. Because it does not require supplementation with serum or other undefined components, Gibco® CD BHK-21 Production Medium eliminates the risks associated with serum, while allowing you to achieve consistent virus titers and simplifying downstream purification.

cGMP Manufacturing
Gibco® CD BHK-21 Production Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Expi293F™ GnTI- Cells Gibco™

The Expi293F GnTI- cell line is derived from engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity and therefore lack complex N-glycans. It is a powerful tool for the high-yield expression of homogeneously glycosylated recombinant proteins.

Features of Expi293F GnTI- cells include:
• Suspension, high-density culture in Expi293 Expression Medium
• Homogeneous N-glycosylation of expressed proteins
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• Significantly higher protein yields than other HEK293 GnTI- cell line alternatives

Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in Expi293 Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293 Expression Medium.

RPMI 1640 Medium Gibco™

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red

The complete formulation is available.

Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g ⁄ L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Product use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco® RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical RPMI 1640 product made in our Grand Island facility (11875-093). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Macrophage-SFM (1X) Gibco™

Macrophage-SFM is a serum-free medium designed for the culture of human peripheral blood monocytes and macrophages (1).

Contains L-glutamine, but no antibiotics.

CTS™ Neurobasal™-A Medium Gibco™

CTS Neurobasal-A Medium is designed with the translational stem cell researcher in mind. It can be used for long-term maintenance and maturation of pure post-natal and adult brain neurons without the need for an astrocyte feeder layer when used with CTS B-27 supplements.

CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485– and ISO 9001–certified.

Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.

For other applications, we provide alternative formulations of CTS Neurobasal media and CTS B-27 supplements.

For established protocols using Neurobasal media, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

VP-SFM AGT™ Medium Gibco™

VP-SFM AGT™ is a serum-free, ultra-low protein medium in an easy-to-use granular format. It is designed for the growth of VERO cells for virus production and for the growth of COS-7, MDCK, BHK-21 and Hep-2 cells for recombinant protein production.
Advanced Granulation Technology = AGT

HepExtend™ Media Kit Gibco™

The HepExtend™ Media Kit contains the new HepExtend Supplement (50X), as well as William's E Medium and Primary Hepatocyte Maintenance Supplement, providing the three components needed to optimize long term hepatocyte culture. HepExtend Supplement has been optimized for use with cryopreserved primary hepatocytes to improve cell viability, function and number of days in culture. This enables researchers to perform metabolic and toxicity experiments not achievable using standard culture conditions. Simply add the HepExtend Supplement and Primary Hepatocyte Maintenance Supplement to the William’s E Medium for a complete solution. The HepExtend supplement does not contain any small molecules or fetal bovine sera, components that are known to interfere with primary hepatocyte function.

Enabling—offers maximum cell viability and cell health when culturing primary plateable hepatocytes
Extended cell survival—days in culture can be extended to >10 days while maintaining normal cell morphology and bile canaliculi
Consistent—manufactured in a cGMP compliant facility using high quality materials

Maintain P450 activity
The HepExtend Media Kit can be used to help ensure metabolically competent hepatocytes for at least 10 days for use in downstream ADME/Tox applications not achieved with standard 5-day culture systems.

Enhanced gene expression
Using the components in the HepExtend Media Kit to culture primary human hepatocytes increases the basal gene expression of many key genes involved in drug metabolism and transport.

Retain inducibility
Nuclear receptor activation and gene inducibility are retained in cultures treated with HepExtend supplemented media.

MEM α, nucleosides, powder Gibco™

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco® MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:

With Without
• Ribonucleosides • Sodium Bicarbonate
• Deoxyribonucleosides
• L-glutamine
• Phenol Red

The complete formulation is available.

Gibco® MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.

cGMP Manufacturing and Quality System
Gibco® MEM α is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco® MEM α product made in our Grand Island facility (11900-024). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

CD BHK-21 Production Medium Gibco™

Gibco® CD BHK-21 Production Medium is a chemically defined, protein-free cell culture medium. It is designed to support the growth of BHK-21 cells in serum-free suspension culture. This medium is suitable for large-scale manufacturing applications.

CD BHK-21 Production Medium is manufactured as follows:
WithWithout
• L-glutamine• Phenol Red
• Dry powder formatProtein hydrolysates

Gibco® CD BHK-21 Production Medium is the first cell culture medium supporting complete serum-free and protein-free processes for BHK-21 cells. The dry powder format is easy to reconstitute, since it has no hydrolysates in the formulation. Adapting cells from serum-containing media is a rapid and simple procedure. BHK-21 cells can achieve high cell densities and maintain high cell viability in this medium.

Gibco® CD BHK-21 Production Medium is suitable for large-scale suspension culture. Because it does not require supplementation with serum or other undefined components, Gibco® CD BHK-21 Production Medium eliminates the risks associated with serum, while allowing you to achieve consistent virus titers and simplifying downstream purification.

cGMP Manufacturing
Gibco® CD BHK-21 Production Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Expi293™ Expression Medium Gibco™

Gibco Expi293 Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F cells in the Expi293 Expression System for scalable transient protein expression.

• Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
• Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine transfection reagent
• Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
• Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10L in disposable bioreactors
• Does not contain phenol red

Expi293 Expression Medium is formulated with GlutaMAX-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293 Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.

Product use
Customers using Expi293 Expression Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

B-27™ Electrophysiology Kit Gibco™

The B-27® Electrophysiology Kit contains Neurobasal® Medium, Electro and B-27® Supplement, Electro, both specifically developed to enhance network spike rates in cultured neurons when compared with other media and supplement combinations. Electrophysiology is the fundamental technique to assess such electrical activities to study the functions and dysfunctions in cultured neurons.

Using the B-27® Electrophysiology Kit in your neuronal studies offers:

• Increased synapse densities
• More spontaneous spike rates
• Improved multielectrode array efficiency

More Synapses, More Signal, Simpler Testing
The B-27® Electrophysiology Kit is an improvement to Neurobasal® and B-27® combination for cultured networks. Hippocampal and cortical neurons cultured in this media system show an increased density of synapses and neurotransmitter receptors which produce higher spike rates in neural networks, helping to simplifying your study.

Relevant Results for Your Electrophysiology Studies
Using Invitrogen® B-27® Electrophysiology Kit, allows you to get more relevant results when studying neuron physiology and the biochemical basis of normal and disease processes. The B-27® Electrophysiology Kit can yield better results in other electrophysiological studies, including neurons used in multielectrode arrays in neurotoxicology and neuropharmacology studies.

For other applications, we offer alternative formulations of B-27® and Neurobasal®.
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