Shop All Additional Selective and Differential Culture Media

CD BHK-21 Production Medium Gibco™

Gibco® CD BHK-21 Production Medium is a chemically defined, protein-free cell culture medium. It is designed to support the growth of BHK-21 cells in serum-free suspension culture. This medium is suitable for large-scale manufacturing applications.

CD BHK-21 Production Medium is manufactured as follows:

With	Without
• L-glutamine	• Phenol Red
• Dry powder format	Protein hydrolysates

Gibco® CD BHK-21 Production Medium is the first cell culture medium supporting complete serum-free and protein-free processes for BHK-21 cells. The dry powder format is easy to reconstitute, since it has no hydrolysates in the formulation. Adapting cells from serum-containing media is a rapid and simple procedure. BHK-21 cells can achieve high cell densities and maintain high cell viability in this medium.

Gibco® CD BHK-21 Production Medium is suitable for large-scale suspension culture. Because it does not require supplementation with serum or other undefined components, Gibco® CD BHK-21 Production Medium eliminates the risks associated with serum, while allowing you to achieve consistent virus titers and simplifying downstream purification.

cGMP Manufacturing
Gibco® CD BHK-21 Production Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

B-27™ Electrophysiology Kit Gibco™

The B-27® Electrophysiology Kit contains Neurobasal® Medium, Electro and B-27® Supplement, Electro, both specifically developed to enhance network spike rates in cultured neurons when compared with other media and supplement combinations. Electrophysiology is the fundamental technique to assess such electrical activities to study the functions and dysfunctions in cultured neurons.

Using the B-27® Electrophysiology Kit in your neuronal studies offers:

• Increased synapse densities
• More spontaneous spike rates
• Improved multielectrode array efficiency

More Synapses, More Signal, Simpler Testing
The B-27® Electrophysiology Kit is an improvement to Neurobasal® and B-27® combination for cultured networks. Hippocampal and cortical neurons cultured in this media system show an increased density of synapses and neurotransmitter receptors which produce higher spike rates in neural networks, helping to simplifying your study.

Relevant Results for Your Electrophysiology Studies
Using Invitrogen® B-27® Electrophysiology Kit, allows you to get more relevant results when studying neuron physiology and the biochemical basis of normal and disease processes. The B-27® Electrophysiology Kit can yield better results in other electrophysiological studies, including neurons used in multielectrode arrays in neurotoxicology and neuropharmacology studies.

For other applications, we offer alternative formulations of B-27® and Neurobasal®.

Leibovitz's L-15 Medium, GlutaMAX™ Supplement Gibco™

Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate. This medium is designed for supporting cell growth in environments without CO₂ equilibration.

This L-15 is modified as follows:

With	Without
• Galactose	• Glucose
• Phenol Red	• HEPES
• GlutaMAX™	• Sodium Bicarbonate
• Sodium Pyruvate

The complete formulation is available.

Gibco® L-15 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® L-15 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

L-15 contains no proteins, lipids, or growth factors. Therefore, L-15 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

Cell Therapy Systems Hibernate™-A CTS™ Medium Gibco™

CTS Hibernate-A Medium is a serum-free, nutrient basal medium for the short-term maintenance of postnatal and adult human tissue in ambient CO₂ conditions.

Quick and easy shipment of human tissues
CTS Hibernate-A Medium can be used for shipment of human postnatal and adult tissue such as brain tumors, umbilical cords, and foreskin at ambient temperature using a simple three step process:

1) Collect tissue in a conical tube containing CTS Hibernate-A Medium (brain tumor cells require additional supplements).
2) Ship tissues in CTS Hibernate-A Medium within 24 to 72 hours on cold packs.
3) Transfer received tissues into medium of choice and initiate cultivation.

CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485– and ISO 9001–certified.

Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.

CTS Hibernate-A Medium can be combined with CTS B-27 Supplements to yield a complete medium.

For other applications, we provide alternative formulations of CTS Hibernate Medium and CTS B-27 Supplements.

For established protocols using Hibernate Medium, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

Liver Perfusion Medium (1X) Gibco™

Liver Perfusion Medium is a buffered, balanced salt solution formulated to cleanse the liver of blood, prevent clotting, and initiate loosening of cell-to-cell contact.

Dispase II, powder Gibco™

Dispase II (neutral protease) is an amino-endo peptidase that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. This sequence is found with high frequency in collagen. Dispase II has a mild proteolytic action that makes it useful for the isolation and routine passaging of primary cells. Dispase II is relatively gentle, dissociates well at physiological temperature and pH, and generally maintains cell membrane integrity.

Gibco® Dispase II is produced in Bacillus polymyxa and packaged as a lyophilized, non-sterile powder for research use in cell or tissue dissociation, and to prevent clumping in suspension cultures. Gibco® Dispase II activity is guaranteed to be greater than 0.5 units/mg.

LHC-8 Medium (1X) Gibco™

LHC serum-free media are the most widely used products for culture of bronchial epithelial cells. For maximum flexibility and convenience, we offer these products with or without supplementation.

LHC Basal Medium (1X), liquid

Applications:
Formulated for the growth of bronchial epithelial cells with further supplements.

Recommended storage conditions:
2° to 8°C.

Shelf life:
12 months

Intended use(s)
Research use only

LHC-8 Medium and LHC-9 Medium

Applications:

Engineered specifically for the growth of bronchial epithelial cells such as IB3-1, S9, and C38, BEAS-2B, BZR, BBM, Het-1A, NHBE (normal human bronchial epithelial cells), and BBE (bovine bronchial epithelial cells) for asthma, allergy, lung cancer, cystic fibrosis, pulmonary, and esophageal cancer research without further supplements.

Performance and quality testing:
Performance tested in a growth assay using BEAS-2B cells.

Recommended storage conditions:
-5°C to -20°C.

Intended use(s)
Research use only

Reference(s):
Pawliczak R., et. al. (2001) J Biol Chem 276, 44613. Zhao Y.L., et. al. (2000) Carcinogenesis 21, 2005. Liu X., et. al. (1998) Am J Physiol 274, L58. Hei T.K., et. al. (1997) Environ Health Perspect 105, 1085. Lechner J.F., and LaVeck, M.A. (1985) J Tissue Cult Methods 9, 43.

Keratinocyte SFM (1X), without calcium chloride Gibco™

Keratinocyte SFM (serum-free medium) is optimized for the growth and maintenance of human keratinocytes and other types of epithelial cells without the need for a feeder layer of cells. The complete medium may be used to cultivate human epidermal keratinocytes for studies involving dermal substitutes, in vitro toxicology, and gene therapy. Keratinocyte SFM without calcium is available to allow researchers to adjust the calcium concentration to meet the demands of their specific application.

Keratinocyte SFM is a complete serum-free medium when supplemented with human recombinant epidermal growth factor (rEGF) and bovine pituitary extract (BPE) at the time of use. Both supplements, rEGF and BPE, are supplied in separate packaging with the basal medium.

CD OptiCHO™ AGT™ Medium Gibco™

CD OptiCHO™ medium is specifically designed to offer high performance and yield with recombinant CHO cells in a chemically defined fed-batch environment. The protein-free, animal origin free formulation is designed to provide consistency and reduces the need to screen for adventitious agents. CD OptiCHO™ medium builds on Gibco® CD CHO’s legacy of consistent cell growth and titers, while offering up to 40% better titer and cell growth over select competitors. Thanks to its low osmolality and advanced CHO CD EfficientFeed™ Liquid Nutrient Supplement Kit feeding options, CD OptiCHO™ medium is capable of gaining 2-5 fold increases in fed-batch productivity over batch culture.

When to Use CD OptiCHO™ Medium
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt

When to Consider Another Gibco® Medium

Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing

Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)

Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)

Liquid Media Formulations
Gibco® AGT™ media are also available in a liquid format that offers the same performance and formulation as the AGT™ media format. Gibco® liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.

Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

Dynamis™ AGT™ Medium Gibco™

Gibco® Dynamis™ AGT™ dry format medium is specifically designed to offer the highest batch and fed-batch culture performance and yield with recombinant CHO cells in a chemically defined environment. The chemically defined, protein-free, animal origin component-free formulation provides the power to achieve high titers, start process development faster, and streamline or simplify transfer to manufacturing scale.

Power to achieve higher titers
Dynamis™ AGT™ Medium is fortified to help maximize recombinant CHO cell growth and protein expression in suspension batch culture, offering 170% greater production than select competitive media with simple glucose supplementation by day 14. Cultures grown in Dynamis™ AGT™ Medium extend to even higher titers demonstrating good cell viability out to day 21. This medium can also provide up to 60% greater fed-batch growth and production over glucose-only fed-batch culture for selected cell lines and applications when used with EfficientFeed™ C+ AGT™ Supplement.

Power to start process development faster
As a fortified medium, Dynamis™ AGT™ provides the power to get to a high cell density over 10 million cells per mL faster than competitive products and sustains that cell density longer by just maintaining glucose levels. The viable cell growth achieved make it a good choice for scale-up if you have started with CD FortiCHO™ medium. When paired with EfficientFeed™ C+ AGT™ Supplement, it provides a fast and efficient way to develop a process.

The power to streamline transfer to manufacturing
By choosing Dynamis™ AGT™ Medium, you obtain not only the benefits of the medium formulation, but also the benefits of AGT™ dry format technology. This all-in-one product is designed to simplify scale-up and manufacturing by being easy to reconstitute (just add water), with no adjustments of pH or osmolarity necessary. This can help provide efficiencies through supply chain simplicity, reconstitution simplicity, and therefore help reduce expense.

Use Dynamis™ AGT™ Medium when:
• The cell line you are using is a transfected CHO K1, GS CHO, or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing

When to consider another Gibco® medium

Consider CD OptiCHO™ Medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO, or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is hard to grow or adapt

Consider CD CHO Medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)

Consider CD DG44 Medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)

Design your own media specific to your needs
Whether it’s a bench-level experiment or large-scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services, you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

Gibco® is a registered trademark of ThermoFisher Scientific Inc. Dynamis™ and CD FortiCHO™ are trademarks of Thermo Fisher Scientific Inc., ActiCHO(TM) is a trademark of GE Healthcare Biosciences AB, BalanCD(R) is a registered trademark of Irvine Scientific Sales Co., Cellvento(R) is a registered trademark of Merck KGAA, EXCELL(R) is a registered trademark of SAFC, HyCell(R) is a registered trademark of GE Healthcare Biosciences AB, and PowerCHO(TM) is a registered trademark of Lonza.

CTS™ OpTmizer™ T-Cell Expansion SFM, no phenol red, bottle format Gibco™

Gibco CTS OpTmizer T-Cell Expansion SFM supports the growth and expansion of human T lymphocytes. It is a complete serum-free, xeno-free medium consisting of CTS OpTmizer T-Cell Expansion Basal Medium and CTS OpTmizer T-Cell Expansion Supplement, which are mixed together prior to use.

CTS OpTmizer T-Cell Expansion SFM is available with and without phenol red. This version of CTS OpTmizer SFM lacks phenol red, and is available in both bottle and bag format.

Features of CTS OpTmizer T-Cell Expansion SFM include:
• Supports high-density T cell culture (>4 x 10⁶ CD3+ T-cells/mL)
• Supports T-cell activation using Dynabeads magnetic beads, soluble antibodies, and stimulatory antibody-presenting cell protocols
• Similar phenotype, function (e.g., cytokine secretion profile), and viability to T-cells cultured with conventional human AB serum-supplemented medium
• Supports a T-cell phenotype similar to human serum-supplemented medium
• Demonstrates enhanced efficacy and persistence of CART-19 cells when grown in medium supplemented with CTS Immune Cell Serum Replacement (ICSR)

Demonstrated functional performance
Cells grown in CTS OpTmizer T-Cell Expansion SFM with or without human serum demonstrate good cytokine production profiles in polyclonally activated T cells. CTS OpTmizer T-Cell Expansion SFM maintains the desired the CD62L+ central memory T-cell phenotype.

GMP manufactured
The primary manufacturing site for CTS OpTmizer T-Cell Expansion SFM is FDA-registered and has an ISO13485-certified quality management system. The methods and controls used for the manufacturing are in conformity with current Good Manufacturing Practices for medical devices, 21 CFR Part 820, of the regulation. Alternate manufacturing sites are available, providing risk mitigation by helping to ensure security of supply.

Versatility
CTS OpTmizer T-Cell Expansion SFM has been widely adopted in clinical T-cell research applications including the culture of CAR T-cells. It works with multiple culture formats, including culture flasks, culture bags, and rocking bioreactor systems. CTS OpTmizer T-Cell Expansion SFM was designed to support expansion of cultured human T-cells without additional serum supplementation. Increased performance is generally observed when supplemented with CTS Immune Cell Serum Replacement.

What you get
CTS OpTmizer T-Cell Expansion SFM includes two components: 1 liter of CTS OpTmizer T-Cell Expansion Basal Medium and 26 mLs of CTS OpTmizer T-Cell Expansion Auxiliary Concentrated Medium, which are mixed together to create the complete medium. Full traceability documentation such as Certificate of Origin and Certificate of Analysis are available.

Medium 199, Earle's Salts (10X) Gibco™

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Medium 199 is modified as follows:

With	Without
• Phenol Red	• HEPES
• L-glutamine	• Sodium Bicarbonate

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco® Medium 199 product made in our Grand Island facility (11825-015). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Concentrated forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Cell Therapy Systems Hibernate™-E CTS™ Medium Gibco™

CTS Hibernate-E Medium is a serum-free, nutrient basal medium for the short-term maintenance of embryonic human tissue in ambient CO₂ conditions.

Quick and easy shipment of human tissues
CTS Hibernate-E Medium can be used for shipment of human embryonic tissue such as brain tumors, umbilical cords, and foreskin at ambient temperature using a simple three step process:

1) Collect tissue in a conical tube containing CTS Hibernate-E Medium (brain tumor cells require additional supplements).
2) Ship tissues in CTS Hibernate-E Medium within 24 to 72 hours on cold packs.
3) Transfer received tissues into medium of choice and initiate cultivation.

CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485– and ISO 9001–certified.

Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.

CTS Hibernate-E Medium can be combined with CTS B-27 Supplements to yield a complete medium.

For other applications, we provide alternative formulations of CTS Hibernate Medium and CTS B-27 Supplements.

For established protocols using Hibernate Medium, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

Medium 200 Gibco™

Medium 200 and phenol red-free Medium 200 (Medium 200PRF) are sterile, liquid tissue culture media intended for use as one component in a complete culture environment for normal human large vessel endothelial cells. When supplemented with LSGS or LSGS Kit, these media will support the plating and proliferation of endothelial cells at culture densities between 2.5 x 10³ cells/cm² and 1 x 10⁵ cells/cm². Additional applications may include primary isolation of endothelial cells.

Both Medium 200 and Medium 200PRF are basal culture media containing essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. These media do not contain antibiotics, antimycotics, hormones, growth factors, or proteins. These media are HEPES and bicarbonate buffered and designed for use in an incubator with an atmosphere of 5% CO²/95% air. To support the plating and proliferation of human large vessel endothelial cells, these media must be supplemented with Low Serum Growth Supplement (LSGS) or Low Serum Growth Supplement Kit.

Medium 154CF/PRF, Kit Gibco™

A sterile, liquid medium for the culture of human epidermal keratinocytes. Medium 154CF⁄PRF is Medium 154 prepared without calcium chloride or phenol red. Calcium chloride is provided as a separate component with each bottle of medium. This basal medium requires the addition of HKGS (S-001-5) or HKGS Kit (S-001-K) prior to use.