Shop All Additional Selective and Differential Culture Media

FunctionMAX™ TiterEnhancer uses a new technology to combine key nutrients in a highly concentrated (150 g/L), pH-neutral, chemically-defined, AOF (animal origin-free) functional additive that is designed to reinforce existing feeds and amplify the productivity of standard high cell density fed-batch platforms. FunctionMAX™ TiterEnhancer is available in both this liquid format and an AGT™ dry format and is stable for over 1 year. FunctionMAX™ TiterEnhancer offers:

• Up to double prior fed-batch titers
• Highly concentrated ingredients
• Easy to use pH-neutral format
• Specialized delivery of ingredients found in base media—nothing exotic
• Use that is economical

When to Choose FunctionMAX™ TiterEnhancer:

• If you have a drop off in specific productivity late in a high density fed-batch culture, FunctionMAX™ TiterEnhancer may be able to improve your titers
• If you have an extremely low peak cell density fed-batch culture, you may want to consult with PD-Direct® Services for advice on cell adaptation and/or fed-batch optimization
• If you have no drop off in specific productivity late in a high density fed-batch culture and still need higher titers, you may want to consult with PD-Direct® Services for advice on fed-batch optimization

Up to Double Prior Fed-batch Titers
We have demonstrated 35–120% product titer improvements when FunctionMAX™ TiterEnhancer was added on top of existing processes or as a partial feed replacement (Figures 1–3), resulting in a lower total proportion of feed addition. These increases were achieved primarily by maintaining specific productivity throughout each fed-batch process. Improvements were seen immediately and without process optimization. We have also shown that low-volume addition of FunctionMAX™ TiterEnhancer was sufficient to improve titers for several processes using different commercially available media and feeds, with those having higher cell densities seeing the most benefit. This additive is not designed to completely replace other feeding supplements that provide additional ingredients not found in FunctionMAX™ TiterEnhancer.

Highly Concentrated Ingredients
The technology used to deliver this functional additive promises to transform recombinant drug bioprocessing by raising key ingredient concentrations to levels that are out of reach using conventional approaches. A concentration of 150 g/L is achieved with FunctionMAX™ TiterEnhancer (compared to 20–25 g/L for most basal media), and it has been shown to be stable in dry or liquid format for over 1 year.

Easy-to-Use pH-neutral Format
Reconstitution of the AGT™ dry format is surprisingly simple (add water and stir), with all components going into solution. There is no pH adjustment required, and being pH neutral it is safer in handling and use than extreme pH alternatives. As a pH neutral additive, this product will help avoid bioreactor complications often seen with extreme pH supplements. Further, the liquid is very stable—so much so that the liquid format version is made from the same AGT™ dry format material that is also available for purchase. The liquid is provided for small-scale trials and the AGT™ dry format for seamless scale-up in use.

Specialized Delivery of Ingredients Found in Base Media—Nothing Exotic
FunctionMAX™ TiterEnhancer does not introduce new ingredients to your fed-batch process; it simply provides the same key ingredients found in basal media that the cells need late in culture to maintain their productivity. Further, the technology used to combine these ingredients does not introduce any additional ingredients. Nor does it contain any growth factors or undefined components like hydrolysates. A DMF (drug master file) reference can be requested for this product for assurance of regulatory authorities that there are no substances or ingredients in the formulation that might create regulatory concerns.

Economies in Use
FunctionMAX™ TiterEnhancer is designed to deliver highly concentrated and high value addition to your fed-batch process. Consider that it provides 7-fold the ingredient concentration of a basal medium and can provide up to a doubling of titer. Further, in normal use at about 10% of working volume, the actual price per liter of use is similar to that of basal media.

VP-SFM AGT™ is a serum-free, ultra-low protein medium in an easy-to-use granular format. It is designed for the growth of VERO cells for virus production and for the growth of COS-7, MDCK, BHK-21 and Hep-2 cells for recombinant protein production.
Advanced Granulation Technology = AGT

VP-SFM AGT™ is a serum free, ultra low protein medium (5 µg/ml) in a new easy-to-use granular format. VP-SFM AGT™ is designed for the growth of VERO cells for virus production. It is also suitable for the growth of COS-7, MDCK, BHK-21 and Hep2 cells and for recombinant protein production.

FreeStyle™ F17 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation. This is a multi-platform medium compatible with a range of transient expression systems. The addition of 4–8 mM L-glutamine or GlutaMAX™ supplement is required before use. Gibco® FreeStyle™ F17 Expression Medium features:

• Little or no required adaptation
• Ability to support suspension growth and transfection of 293-F and CHO cells
• Animal origin–free, protein-free, and chemically defined formulation
• Scalability in spinner flasks and bioreactors

Little or no required adaptation
Gibco® 293-F, 293-H, FreeStyle™ 293-F, and CHO-S cells in suspension can be cultured directly into Gibco® FreeStyle™ F17 Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® FreeStyle™ F17 Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations.

Ability to support suspension growth and transfection of 293-F and CHO cells
Gibco® FreeStyle™ F17 Medium provides equal or better performance compared to Gibco® FreeStyle™ 293 Expression Medium and Gibco® FreeStyle™ CHO Medium. Gibco® FreeStyle™ F17 Medium supports 293-F cells at a peak density of 6–7 × 10⁶ viable cells per mL, and supports CHO-S cells at a peak density of 8–9 × 10⁶ viable cells per mL. Cells cultured in Gibco® FreeStyle™ F17 can be transfected using a wide variety of transfection reagents, including lipid-based transfection reagents, such as 293fectin™ and Freestyle™ MAX Reagent. Protein expression typically exceeds 75–150 µg/mL IgG in 293-F cells.

Animal origin–free, protein-free, and chemically defined formulation
Gibco® FreeStyle™ F17 Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

Scalability in spinner flasks and bioreactors
Protein production using Gibco® FreeStyle™ F17 Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco® FreeStyle™ F17 Medium with additional Pluronic® F-68 to avoid sheer stress in the culture.

cGMP manufacturing and quality system
Gibco® FreeStyle™ F17 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Fischer's medium was originally developed by Glen Fischer at the National Cancer Institute (Bethesda, MD) and formulated for the culture of mouse leukemic cells. Fischer's Medium is primarily used for the culture of hematopeitic cells, such as lymphoblasts and lymphocytes.

This Fischer's medium is manufactured as follows:
The complete formulation is available.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

cGMP Manufacturing
Gibco® Fischer's medium is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Fischer's medium contains no proteins, lipids, or growth factors. Fischer's medium typically requires supplementation of Fetal Bovine Serum (FBS). Fischer's medium uses a sodium bicarbonate buffer system (1.125 g / L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Dispase II (neutral protease) is an amino-endo peptidase that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. This sequence is found with high frequency in collagen. Dispase II has a mild proteolytic action that makes it useful for the isolation and routine passaging of primary cells. Dispase II is relatively gentle, dissociates well at physiological temperature and pH, and generally maintains cell membrane integrity.

Gibco® Dispase II is produced in Bacillus polymyxa and packaged as a lyophilized, non-sterile powder for research use in cell or tissue dissociation, and to prevent clumping in suspension cultures. Gibco® Dispase II activity is guaranteed to be greater than 0.5 units/mg.

Engineered specifically for culture of human mammary epithelial cells, HuMEC Medium is a serum-free formulation with frozen supplements containing growth factor and other components. This translates to greater experimental validity, more reproducible results, and reduction of lot-to-lot variability and aberrations that can occur with the addition of serum. Human mammary epithelial cells cultured in HuMEC Medium have gene expression profiles consistent with normal mammary epithelial cells, as tested on a cDNA microarray.

Applications:
HuMEC Medium is optimized specifically for the growth of human mammary epithelial cells, and has been performance tested in a growth assay using normal human mammary epithelial cells. HuMEC Medium promotes superior growth of normal human mammary epithelial cells, making it ideal for researchers in breast cancer, drug discovery, and other aspects of breast biology.

Contents and Storage:
HuMEC Ready Medium consists of HuMEC Basal Serum-Free Medium (12753018) supplemented with HuMEC Supplement Kit (12755013). The HuMEC Supplement Kit includes 5 ml of a supplement mix containing epidermal growth factor, hydrocortisone, isoproterenol, transferrin, and insulin, and 25 mg of bovine pituitary extract. Store HuMEC Ready Medium at -5°C to -20°C. Store the supplement mix at -5°C to -20°C.

CHO-S-SFM II, is a complete, serum-free, low-protein (less than 100 µ/ml) medium optimized for the growth and maintenance of Chinese hamster ovary (CHO) cells in suspension culture for production of recombinant proteins. It is supplemented with hypoxanthine and thymidine (the non-supplemented equivalent is also available). This ready-to-use medium is suitable for the suspension culturing of CHO and other cells in batch, continuous, and perfusion culture systems. A CHO-S-SFM II medium Drug Master File (DMF) has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative.

This medium contains components of animal origin. If growth factors or hydrolysates, found in competitor media, are to be avoided, consider CD CHO, CD OptiCHO™ or CD FortiCHO™ media.

When to use CHO-S-SFM II Medium
• Your cells have been successfully cultured in CHO-S-SFM II medium in the past and your process is nearing a regulatory filing (DMF available)

When to Consider Another Gibco® Medium

Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing

Consider CD OptiCHO™ medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt

Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)

Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)

Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

Gibco® Glucose Solution contains D-glucose at a concentration of 200 g/L and is used as a serum-free medium supplement suitable for cell culture. Glucose Solution can be added to a variety of Gibco® cell culture media including but not limited to Dulbecco’s Modified Eagle Medium (DMEM), RPMI 1640, DMEM/F-12, IMDM, and Minimum Essential Medium. Each lot of Glucose Solution is tested for sterility, osmolality, pH, and endotoxin level. We offer a variety of media to which Glucose Solution can be added. Find the right medium using the media selector tool.

cGMP Manufacturing and Quality System
Glucose Solution is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Gibco Dynamis liquid medium is specifically designed to offer the highest batch and fed-batch culture performance and yield with recombinant CHO cells in a chemically defined environment. The chemically defined, protein-free, animal origin component-free formulation provides the power to achieve high titers, start process development faster and streamline or simplify transfer to manufacturing scale.

Power to achieve higher titers
Dynamis Medium is fortified to help maximize recombinant CHO cell growth and protein expression in suspension batch culture, offering 170% greater production than select competitive media with simple glucose supplementation by day 14. Cultures grown in Dynamis Medium extend to even higher titers demonstrating good cell viability out to day 21. This medium can also provide up to 60% greater fed-batch growth and production over glucose-only fed-batch culture for selected cell lines and applications when used with EfficientFeed C+ Supplement.

Power to start process development faster
As a fortified medium, Dynamis provides the power to get to a high cell density over 10 million cells per mL faster than competitive products and sustains that cell density longer by just maintaining glucose levels. The viable cell growth achieved make it a good choice for scale-up if you have started with CD FortiCHO medium. When paired with EfficientFeed C+ Supplement, it provides a fast and efficient way to develop a process.

When to Use Dynamis Medium
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing

When to Consider Another Gibco Medium

Consider CD OptiCHO Medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky,' i.e. hard to grow or adapt

Consider CD CHO Medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)

Consider CD DG44 Medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)

Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

ActiCHO is a trademark of GE Healthcare Biosciences AB, BalanCD is a registered trademark of Irvine Scientific Sales Co., Cellvento is a registered trademark of Merck KGAA, EXCELL is a registered trademark of SAFC, HyCell is a registered trademark of GE Healthcare Biosciences AB, and PowerCHO is a registered trademark of Lonza.

FreeStyle™ 293 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F cells in suspension without adaptation. The medium is complete and ready-to-use, with no supplementation required. Gibco® FreeStyle™ 293 Expression Medium features:

• Complete and ready-to-use format containing GlutaMAX™ supplement
• Ability to support suspension growth and transfection of 293-F cells
• Animal origin–free, protein-free and chemically defined formulation
• Easy use with the FreeStyle™ 293 Expression systems
• Scalability in spinner flasks and bioreactors

Complete and ready-to-use format containing GlutaMAX™ supplement
Gibco® FreeStyle™ 293 Expression Medium is ready to use. It does not require the addition of serum, glutamine, or surfactants. Gibco® FreeStyle™ 293 Expression Medium contains GlutaMAX™ supplement to minimize toxic ammonia build-up and improve cell viability and growth in an easy-to-use format.

Ability to support suspension growth and transfection of 293-F cells
Gibco® 293-F, 293-H, and FreeStyle™ 293-F cells in suspension can be cultured directly into Gibco® FreeStyle™ 293 Expression Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® FreeStyle™ 293 Expression Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations. Cells cultured in Gibco® FreeStyle™ 293 Expression Medium can be transfected using lipid based transfection reagents, such as 293fectin™ reagent and Freestyle™ MAX reagent.

Animal origin–free, protein-free and chemically defined formulation
Gibco® FreeStyle™ 293 Expression Medium is animal origin–free, protein-free and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

Easy use as part of the FreeStyle™ 293 Expression System and FreeStyle™ MAX Expression System for protein production
The FreeStyle™ 293 Expression System is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein (see figure). The FreeStyle™ 293 Expression System combines Gibco® FreeStyle™ 293 Expression Medium with 293fectin™ reagent, a cationic-lipid formulation designed to transfect suspension FreeStyle™ 293-F cells at high efficiency. The FreeStyle™ MAX Expression System is a breakthrough technology for rapid and high-yield mammalian protein production. The FreeStyle™ MAX Expression System combines Gibco® FreeStyle™ Media, FreeStyle™ MAX Reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293-F cells.

Scalability in spinner flasks and bioreactors
Protein production using Gibco® FreeStyle™ 293 Expression Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco® FreeStyle™ 293 Expression Medium with additional Pluronic® F-68 to avoid sheer stress in the culture.

Universal bag packaging
Gibco® universal bags offer flexible packaging that allows you to integrate a single-use, disposable large volume media container with a wide variety of automated cell culture instruments, disposable bioreactor systems, and sterile connection processes, minimizing the need for costly customization.

Product use
Customers using Gibco® FreeStyle™ 293 Expression Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
Gibco® FreeStyle™ 293 Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco® MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.

Gibco® MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B₁₂, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.

cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture Gibco® MEM α at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH. Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.

Gibco® MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂. This product is made with Earle’s salts.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® MEM is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Powder forms of Gibco® L-15 cell culture medium require pH adjustment and filtration at the time of preparation (see protocol for details).

Irradiated Gibco B6-Puro Mouse Embryonic Fibroblasts (MEFs) are puromycin-resistant feeder cells that support the culture of healthy undifferentiated human and mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. No. GSC-6220G, these MEF cells are isolated from C57BL/6J-Tg(pPwL88puro)2EMS/J mice, mitotically arrested by irradiation, and tested to help ensure safety and performance. For other variants of drug-resistant MEFs, see CF6-Neo Mouse Embryonic Fibroblasts, Irradiated or DR4 Mouse Embryonic Fibroblasts, Irradiated. You may also choose from our selection of non-drug-resistant MEFs to suit your application.

• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Perform drug selection with puromycin-resistant cells

Help save time with ready-to-use MEFs
Irradiated B6-Puro MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, as well as expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.

Culture with meticulously tested feeder cells
Each lot of B6-Puro MEFs is tested for bacteria, fungi, mycoplasma, and various mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ES cells to ensure robust and consistent performance. These MEFs have successfully been used with ESC/iPSC lines from other species, but should be tested for compatibility if used for such an application.

Perform drug selection with puromycin-resistant cells
B6-Puro MEFs express puromycin N-acetyltransferase (pac) from a pPWL88puro transgene, making them highly resistant to puromycin. Each lot is tested for tolerance to various puromycin concentrations, and the threshold range for survival is reported in the certificate of analysis to aid in designing experiments. Use these feeder cells to support undifferentiated ESC or iPSC culture while performing puromycin selection.

The B-27 Electrophysiology Kit contains Neurobasal Medium, Electro and B-27 Supplement, Electro, both specifically developed to enhance network spike rates in cultured neurons when compared with other media and supplement combinations. Electrophysiology is the fundamental technique to assess such electrical activities to study the functions and dysfunctions in cultured neurons.

Using the B-27 Electrophysiology Kit in your neuronal studies offers:

• Increased synapse densities
• More spontaneous spike rates
• Improved multielectrode array efficiency

More Synapses, More Signal, Simpler Testing
The B-27 Electrophysiology Kit is an improvement to Neurobasal and B-27 combination for cultured networks. Hippocampal and cortical neurons cultured in this media system show an increased density of synapses and neurotransmitter receptors which produce higher spike rates in neural networks, helping to simplifying your study.

Relevant Results for Your Electrophysiology Studies
Using Invitrogen B-27 Electrophysiology Kit, allows you to get more relevant results when studying neuron physiology and the biochemical basis of normal and disease processes. The B-27 Electrophysiology Kit can yield better results in other electrophysiological studies, including neurons used in multielectrode arrays in neurotoxicology and neuropharmacology studies.

For other applications, we offer alternative formulations of B-27 and Neurobasal.