Shop All Stem Cell Culture Media

StemPro™ MSC SFM XenoFree Gibco™

GIBCO® StemPro® MSC SFM XenoFree has been developed for the growth and expansion of human mesenchymal stem cells (MSCs) and Adipose-derived Stem Cells (ADSCs) under completely serum-free and xenogeneic (xeno)-free conditions. Using StemPro® MSC SFM XenoFree, human MSCs or ADSCs can be expanded for multiple passages while maintaining their multipotent phenotype (i.e. ability to differentiate into osteogenic, chondrogenic and adipogenic lineages). StemPro® MSC SFM XenoFree is cGMP compliant which allows for traceability and manufacturing reliability.

CTS™ StemPro™ HSC Expansion Medium Gibco™

CTS StemPro HSC Expansion Medium was designed to help meet regulatory compliance requirements for ancillary materials and provides more functional cells for cell therapy applications. This xeno-free, serum-free medium is manufactured in accordance with cGMP for medical devices (ISO13485 guidelines). The phenotypes of the hematopoietic stem cells (HSCs) targeted for expansion are the total CD34+ cell population with an emphasis on enrichment of the CD34+CD90+CD45RA- cell subset. CTS StemPro HSC Expansion Medium can be used to study hematopoietic cells from human mobilized peripheral blood, bone marrow, or cord blood.

CTS StemPro HSC Expansion Medium consists of a liquid basal medium and a frozen supplement that is added to the basal medium at the time of use. Growth factors or cytokines are not included.

Features of CTS StemPro Expansion Medium include:
• Manufactured following cGMP for medical devices and designed to help meet regulatory requirements for ancillary materials used in cell and gene therapy manufacturing
• Superior expansion of total CD34+ cell population and enrichment of CD34+CD90+CD45RA- subset
• Maintenance of in vivo functionality as demonstrated in animal engraftment models

Designed to help meet regulatory requirements
CTS StemPro HSC Expansion Medium is manufactured in accordance with cGMP for medical devices and supported by a drug master file (DMF) and regulatory support file (RSF) to assist in global regulatory filings and approvals. The formulation is serum-free and xeno-free, and USP/EP grade components are preferentially chosen where possible. In addition, each lot undergoes quality control testing to ensure safety and performance for cell and gene therapy applications.

Superior expansion
CD34+ cells and the CD34+CD90+CD45RA- subset are found in low proportions in mobilized peripheral blood, bone marrow, and cord blood. They are important for proper hematopoietic and immune function and are studied in a variety of research areas, including transplantation biology, stem cell biology, and hematopoietic development. CTS StemPro HSC Expansion Medium enables superior expansion of not only the total CD34+ compartment, but also enriches for CD34+CD90+CD45RA- stem cells, providing more cells to work with (see figures below).

Maintenance of functionality
Functionality of expanded HSCs is critical to success in cell and gene therapy applications. The most widely accepted method for functional testing of HSCs is engraftment in mouse models. The theory is that if human cells persist after transplantation into immunodeficient mice, the cells have demonstrated long term repopulation and differentiation capacity, which are both defining factors of stem cells. See the figure below for representative mouse model engraftment data of CD34+ cells expanded in CTS StemPro HSC Expansion Medium.

CF1 Mouse Embryonic Fibroblasts, MitC-treated Gibco™

Mitomycin C (MitC)-treated Gibco CF1 Mouse Embryonic Fibroblasts (MEFs) are feeder cells that are ideal for supporting healthy undifferentiated human and mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) in culture. Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. Nos. GSC-6001M and GSC-6201M, these widely-used MEF cells are isolated from outbred CF1 mice, mitotically arrested by MitC-treatment, and tested to ensure safety and performance.

• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Rely on a widely-used product

Help save time with ready-to-use MEFs
Irradiated CF1 MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, and expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.

Culture with meticulously tested feeder cells
Each lot of CF1 MEFs is tested for bacteria, fungi, mycoplasma, and mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ES cells to ensure robust and consistent performance. These MEFs have successfully been used with ESC/iPSC lines from other species, but should be tested for compatibility if used for such an application.

Rely on a widely-used product
These CF1 MEFs are used in many labs worldwide and have been cited in various publications. They have been successfully used to support undifferentiated pluripotent stem cells at densities ranging from 2 x 104–5.3 x 104 cells/cm2.

CTS™ AIM-V™ Medium, without phenol red, without antibiotics Gibco™

Gibco CTS AIM V SFM (Therapeutic Grade) is the first commercially available, defined, serum-free medium (SFM) for the proliferation and/or manipulation of T cells and dendritic cells manufactured in compliance with cGMP. It contains L-glutamine and is formulated without phenol red and without antibiotics. CTS AIM-V Medium can be supplemented with human AB serum or CTS Immune Cell SR and various cytokines or growth factors depending on the application.

• cGMP manufactured in compliance with 21 CFR part 820
• Supports expansion of human peripheral blood T-cells including CAR T cells
• Supports expansion of activated human dendritic cells and other immune cells
• Proven clinical use as an ancillary reagent in Provenge™ (sipuleucel-T), the first therapeutic cancer vaccine approved by the FDA [1]
• Adherence to supplier-related responsibilities of USP <1043>*

Security of supply
CTS AIM-V Medium is a defined serum-free formulation that contains no bovine-derived components at the primary component level. The product can be scaled to meet future clinical and commercial demand. Alternate manufacturing sites are available as further risk mitigation.

CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform to cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485- and ISO 9001–certified. Gibco CTS products follow USP <1043> 'ancillary materials for cell, gene, and tissue-engineered products' within the responsibilities applicable to a supplier.*

Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.

Immune cell applications
CTS AIM-V Medium is cited in many peer-reviewed journals and used in multiple immune cell clinical applications such as activated dendritic cells. CTS AIM-V Medium supports expansion of T cells, T cell subsets, and virus-specific T cells. CTS AIM-V Medium can also be used to support natural killer cells combined with feeders. Each lot of CTS AIM-V Medium is pre-qualified using Jurkat cells, an immortalized human T lymphocyte line, to help ensure consistent performance.

What you get
CTS AIM-V Medium is available with L-glutamine, but phenol red, gentamicin sulfate, and streptomycin sulfate have been removed to meet global regulatory requirements for ancillary materials used in cell therapy manufacturing. For convenience, CTS AIM-V Medium is available in a 1000-mL bottle for small volume users as well as a 10-L bag for larger volume users interested in closed systems.

*Other aspects of USP <1043> will be the responsibility of the end-user to assess. Thermo Fisher Scientific cannot fulfill USP <1043> in regards to application and therapy-specific aspects (e.g., residual assessment and removal of the AM).

1. Madan RA, Gulley JL. Sipuleucel-T: harbinger of a new age of therapeutics for prostate cancer. Expert Rev Vaccines. 2011 10(2):141-50.

CTS™ Essential 8™ Medium Gibco™

CTS Essential 8 Medium is a fully defined feeder-free medium formulated for the growth and expansion of both embryonic and induced human pluripotent stem cells (PSCs). Essential 8 Medium is the most defined PSC medium as described in the original publication by Guokai Chen et al. in the laboratory of James Thomson. CTS Essential 8 Medium enables the transition from bench to clinic through the direct replacement of research-use-only (RUO) Essential 8 Medium.

CTS Essential 8 Medium:
• Reduces risks through a formulation with components not directly derived from animals
• Facilitates customer regulatory filings with cGMP manufacturing, traceability of raw materials, and available documentation, including certificates of origin, drug master files, and regulatory support files
• Simplifies the transition from bench to clinic with the same 8-component formulation as RUO Essential 8 Medium, but with components not directly derived from animals

Reduces risks from animal-origin material
CTS Essential 8 Medium contains no animal- or human-derived components at the primary component level. It is a fully defined medium for pluripotent stem cell culture: competitors’ similar-grade products contain human serum albumin (HSA), which brings additional risks in a clinical environment.

Facilitates regulatory filings
Gibco CTS products are manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing site is ISO 13485-certified. Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available for CTS Essential 8 Medium.

Simplifies transition from bench to clinic
Like RUO Essential 8 Medium, CTS Essential 8 Medium contains only the eight essential components needed for stem cell culture, but with components not directly derived from animals. Combined with cGMP manufacturing, this simple, fully defined formulation improves consistency compared to other media that contain over 20 highly variable ingredients. The close similarity between Essential 8 Medium and CTS Essential 8 Medium enables a smooth transition from one medium to the other.

CTS Essential 8 Medium is commercialized in partnership with Cellular Dynamics International.

CTS™ LV-MAX™ Production Medium Gibco™

Gibco CTS LV-MAX Production Medium is a chemically-defined, serum-free, protein-free medium validated for recombinant lentiviral vector production. In addition to supporting high cell density suspension cultures of CTS Viral Production Cells (HEK293F-derived cells), this medium is suitable for preclinical-, clinical-, and commercial-scale transient transfection and lentiviral production when used with the CTS LV-MAX Transfection Kit. CTS LV-MAX Production Medium undergoes stringent QC testing to ensure it is appropriate for the manufacturing of cell and gene therapy applications.

CTS LV-MAX Production Medium may be applicable for other recombinant viral vector production that uses a HEK293 medium.

CTS LV-MAX Production Medium features include:
• Supports CTS Viral Production Cells to achieve densities of over 1 x 107 cells/mL with minimal cell clumping
• Enables high viral titers of up to 1 x 108 TU/mL (unconcentrated lentiviral vectors–GFP)
• Scalable protocol for 96-deep well blocks, flasks, and bioreactors
• Regulatory-compliant medium manufactured in conformity with cGMP for medical devices, 21 CFR, Part 820, and certified to the ISO 13485. Drug Master File and Regulatory Support File available upon request.

CTS LV-MAX Production Medium is formulated with GlutaMAX-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. The medium contains no human or animal-origin components. It is not recommended for adherent cell cultures. The formulation of this product is identical to research-grade LV-MAX Production Medium, but CTS LV-MAX Production Medium is validated for clinical and commercial therapeutic applications.

Custom configuration and packaging available upon request.

Essential 8™ Adaptation Kit Gibco™

The Essential 8™ Adaptation Kit includes Essential 8™ Medium and recombinant human Laminin 521 (rhLaminin-521) for optimal survival during transition from feeder-dependent to feeder-free culture. rhLaminin-521 has been proven to promote cellular survival under stressful conditions in the absence of small molecule inhibitors. In addition, rhLaminin-521 has been shown to support PSC growth for >20 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. rhLaminin-521 can also be purchased separately.

The Essential 8 Adaptation Kit is:
• Robust–transition cells between feeder-dependent and feeder-free culture systems with minimal effort
• Physiologically relevant–expand cells with minimal differentiation
• Scalable–passage stem cell lines as single cells with no impact to genetic stability

Trouble-free transition
When used with Essential 8 Medium, rhLaminin-521 provides a robust culture matrix that supports pluripotent stem cell adherence and expansion during the transition from feeder-dependent culture systems.

Confident expansion
rhLaminin-521 is expressed in blastocysts and therefore provides an environment mimicking the human stem cell niche.

Easy scale-up
rhLaminin-521 promotes stable pluripotent stem cell maintenance as single cells in the absence of ROCK inhibitors.

MarrowMAX™ Bone Marrow Medium Gibco™

Gibco® MarrowMAX™ Bone Marrow Medium is a fully-supplemented medium developed for the short term culture of bone marrow and other hematopoietic cells for cytogenetic studies and in vitro diagnostic procedures.

Gibco® MarrowMAX™ Bone Marrow Medium features:

• Superior performance
• Ready-to-use format
• Quality and performance testing
• Unique, optimized formulation

Superior performance
Gibco® MarrowMAX™ Bone Marrow Medium outperforms other commercially available media containing Giant Cell Tumor (GCT) conditioned media, providing consistent performance with a higher mitotic index and superior chromosomal morphology (learn more about the superior performance of MarrowMAX™ medium here).

Ready-to-use format
Gibco® MarrowMAX™ Bone Marrow Medium is a frozen, 1X medium, ready to use upon thawing. No supplementation is required. Thawed medium can be stored at 2–8°C for up to 60 days.

Quality and performance testing
Every lot of Gibco® MarrowMAX™ Bone Marrow Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Bone marrow cells from the aspirate of a normal adult donor are cultured for 24 hours in Gibco® MarrowMAX™ Bone Marrow Medium before measuring the mitotic index and chromosome banding resolution. In addition, each lot is tested for sterility, pH, osmolality, mycoplasma and endotoxin.

Unique, optimized formulation
Gibco® MarrowMAX™ Bone Marrow Medium is an optimized RPMI 1640 medium, supplemented with Fetal Bovine Serum (FBS), gentamicin, and L-glutamine. It also contains a novel human stromal cell conditioned medium that consists of a unique blend of hematopoietic growth factors. Gibco® MarrowMAX™ Bone Marrow Medium does not contain GCT conditioned medium.

Product Use
For in vitro diagnostic use.

cGMP Manufacturing and Quality System
Gibco® MarrowMAX™ Bone Marrow Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

iPSC Lab Starter Kit with Reprogramming Gibco™

The Gibco™ iPSC Lab Starter Kit with Reprogramming provides researchers initiating pluripotent stem cell research with the required reagents at a significant discount over purchasing them separately. This package solution is appropriate for labs that plan to perform reprogramming of donor-derived fibroblasts . For labs that are starting from cryopreserved pluripotent stem cells, please see the Gibco™ iPSC Lab Starter Kit, which does not include reprogramming reagents.

The iPSC Lab Starter Kit with Reprogramming includes the following products:

Reprogramming:
• CytoTune-iPS 2.0 Sendai Reprogramming Kit, Cat. No. A16517, 1 kit
• High glucose DMEM with GlutaMAX™-I, Cat. No. 10569-044, 40 x 500 mL
• Gibco™ Mouse Embryonic Fibroblasts (Irradiated), Cat. No. A24903, 11 vials
• MEM Non-Essential Amino Acids Solution (100X), Cat. No. 11140-050, 100 mL
• Fetal Bovine Serum, ES Cell-Qualified, Cat. No. 16141-061, 2 x 100 mL
• Attachment Factor, Cat. No. S-006-100, 2 x 100 mL
• 2-Mercaptoethanol (55 mM), Cat. No. 21985-023, 50 mL
• DMEM/F-12 with GlutaMAX™, Cat. No. 10565-018, 5 x 500 mL
• KnockOut™ Serum Replacement, Cat. No. 10828-010, 3 x 100 mL
• FGF-basic (AA 1-155) Recombinant Human Protein, Cat. No. PHG0264, 4 vials
• Collagenase Type IV, Cat. No. 17104-019, 1 vial
• Tra-1-60 AlexaFluor™ 488 Conjugate for Live Staining, Cat. No. A25618, 1 kit

PSC feeder-free transition and expansion:
• Essential 8™ Adaptation Kit, Cat. No. A25953, 1 kit
• Essential 8™ Medium, Cat. No. A1517001, 4 x 500 mL
• Geltrex™ hESC-qualified Basement Membrane Extract, Cat. No. A1413302, 2 x 5 mL
• Vitronectin (VTN-N), Cat. No. A14700, 3 x 1 mL
• Versene Solution, Cat. No. 15040066, 100 mL
• Dulbecco’s Phosphate Buffered Saline without Ca2+/Mg2+, Cat. No 14190250, 20 x 500 mL

CTS™ KnockOut™ SR XenoFree Medium Gibco™

GIBCO® CTS™ KnockOut™ SR XenoFree Medium, when formulated as a complete medium, enables the growth and expansion of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) in a cell culture medium containing only human-derived or human recombinant proteins, to facilitate the transition of hESC and hiPSC research from the bench to the clinic.

The CTS™ product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications.

• Defined, xenofree medium for hESC⁄iPSC stem cell culture.
• First-to-market xenofree hESC⁄iPSC product intended for Ex Vivo tissue and cell culture processing applications.

CTS™ KnockOut™ SR XenoFree Medium does not contain bovine or other non-human, animal-derived components. Besides pluripotent stem cell culture expansion and maintenance, CTS™ KnockOut™ SR XenoFree Medium can be used for hESC⁄hiPSC cryopreservation, derivation, and differentiation studies.

GIBCO® CTS™ products are of high quality, and are supplied with harmonized documentation such as Certificates of Analysis, Certificates of Origin, and access to authorization letters for our Drug Master Files, as appropriate.

For human ex vivo tissue and cell culture processing applications. CAUTION: Not intended for direct administration to humans or animals.

Hibernate™-E Medium Gibco™

Hibernate Medium is a CO2-independent nutrient medium for the maintenance of neural cells, tissue, and tissue slices. Hibernate-E Medium is optimized for embryonic cells or tissue in ambient carbon dioxide levels.

Hibernate Medium enables neurons to be maintained in ambient CO2 levels for up to 48 hours. This provides scientists with greater convenience and control while running bench-top live cell experiments, such as microscopy, flow cytometry, and other physiology studies. Hibernate Medium also allows for the preservation of viable brain tissue before processing for up to a month with refrigeration and maintenance of tissue slices.

Hibernate Medium may be combined with B-27 Supplements to yield a complete medium.

For other applications, we provide alternative formulations of Hibernate Medium and B-27 Supplements.

For established protocols using Hibernate Medium, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

StemPro™-34 SFM (1X) Gibco™

StemPro®-34 SFM is a serum-free medium specifically formulated to support the development of human hematopoietic cells in culture (1). StemPro®-34 SFM can be used to study hematopoietic cells from bone marrow, peripheral blood, or neonatal cord blood. StemPro®-34 SFM is performance-tested on normal human bone marrow. It is manufactured with components of human, recombinant or synthetic origin.

StemPro®-34 SFM consists of a basal liquid medium and frozen supplement (StemPro®-Nutrient Supplement) which is added at the time of use.

Neurobasal™-A Medium, minus phenol red Gibco™

Neurobasal Medium minus phenol red is a basal medium designed for long-term maintenance and maturation of pure post-natal and adult brain neurons without the need for an astrocyte feeder layer when used with Gibco B-27 supplements. Use this modified formulation of Neurobasal Medium when the presence of phenol red will interfere with your results.

Neurobasal Medium is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of Neurobasal media and B-27 supplements.

For established protocols using Neurobasal media, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

CTS™ Essential 6 Medium

CTS Essential 6 Medium is a cGMP-manufactured, xeno-free, feeder-free medium that supports the reprogramming of somatic cells and the spontaneous or directed differentiation of human pluripotent stem cells (PSCs). The formulation is based on a medium originally developed by Guokai Chen, et al., in the laboratory of James Thomson (1). With only six essential components, CTS Essential 6 Medium helps minimize variability. To complete your PSC workflow with a matching culture medium for the growth and expansion of PSCs, use CTS Essential 8 Medium.

CTS Essential 6 Medium:
• Provides a flexible format to suit different applications
• Facilitates regulatory filings through cGMP manufacturing and the availability of documentation
• Simplifies the transition from bench to clinic through the direct replacement of RUO Essential 6 Medium

Provides a flexible format
CTS Essential 6 Medium does not contain bFGF or TGFβ and so can support the formation of embryoid bodies. This medium can support the directed differentiation of various cell types and somatic cell reprogramming using a variety of methods.

Facilitates regulatory filings
CTS Essential 6 Medium is manufactured at a site that uses methods and controls that conform with cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing site is ISO 13485-certified. Full documentation traceability and convenient access to our Drug Master File (DMF) are available for CTS Essential 6 Medium.

Simplifies transition from bench to clinic
CTS Essential 6 Medium contains the same six essential components as RUO Essential 6 Medium, and with its cGMP manufacturing process, this fully-defined formulation helps minimize variability. Like RUO Essential 6 Medium, CTS Essential 6 Medium does not contain bFGF or TGF-beta, which inhibits differentiation and negatively affects reprogramming efficiency, respectively. With its additional safety testing, including adventitious agents, CTS Essential 6 Medium enables a smooth transition from RUO Essential 6 Medium.

Commercialized in partnership with Cellular Dynamics International.

Reference
1. Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, Antosiewicz-Bourget J, Teng JM, Thomson JA. Chemically defined conditions for human iPSC derivation and culture. Nat Methods. 2011 8(5):424-9.

MesenPRO RS™ Medium Gibco™

MesenPRO RS™ Medium is a reduced serum (2%) medium specifically formulated to support the growth of human mesenchymal stem cells (MSCs) in culture. MesenPRO RS™ Medium consistently improves MSC expansion compared with conventional serum-supplemented media. Using MesenPRO RS™ Medium, MSCs can be expanded for multiple passages while maintaining their multipotential phenotype (i.e., differentiation into osteogenic, chondrogenic, and adipogenic lineages).

MesenPRO RS™ Medium consists of:

• Basal liquid medium (MesenPRO RS™ Basal Medium)
• Frozen supplement (MesenPRO RS™ Growth Supplement), which is added at the time of use.

Applications:
Formulated for the growth and expansion of mesenchymal stem cells.
Note: MesenPRO RS™ Medium has been developed for culturing MSCs at greater than clonal densities.

Recommended storage conditions:
• MesenPRO RS™ Basal Medium: Store in the dark at 2 to 8°C
• MesenPRO RS™ Growth Supplement: Store in the dark at -5 to -20°C

Both the Basal Medium and Growth Supplement are guaranteed stable for 24 months when properly stored.

Note: MesenPRO RS™ complete medium (basal, supplement and glutamine) is stable for 15 days when stored in the dark at 2 to 8°C. We do not recommend using the complete medium beyond 15 days.

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